Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,824,197 C→T 6.0% E2346E (GAG→GAA)  pvdL ← non‑ribosomal peptide synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,824,1970CT 6.0% 295.1 / 5.0 99E2346E (GAG→GAA) pvdLnon‑ribosomal peptide synthetase
Reads supporting (aligned to +/- strand):  ref base C (43/50);  new base T (2/4);  total (45/54)
Fisher's exact test for biased strand distribution p-value = 6.86e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.79e-01

TGGGCGCGCAGTACCACGCGCGCACCACACAACAGCGGCACCCACAGGCGCTCGCTGGCAGCGTCGAAATTGATCGAATAGAAATGCAGCTCGCAATCATCGCTGCGCATGCCGAATGCGGCGATCACCGCCTGGCAGTGCATGGCGAACTCGCCATGGCTGACCACCACGCCCTTGGGTTTGCCGGTGGAGCCTGAGGTGTAGATCAGGTAAGCCTGGTGCTGCGGCAGGCTGAGGTTGTCCAGCGGTGCATCGCTGTAGGCCGCCAGGCTGGCGGCATCGTCTTCCAGGCTCCA  >  NC_002947/4824048‑4824343
                                                                                                                                                     |                                                                                                                                                  
tGGGCGCGCAGTACCACGCGCGCACCACACAACAGCGGCACCCACAGGCGCTCGCTGGCAGCGTCGAAATTGATCGAATAGAAATGCAGCTCGCAATCATCGCTGCGCATGCCGAATGCGGCGATCACCGCCTGGCAGTGCATGGCGAAc                                                                                                                                                    >  1:29378/1‑150 (MQ=255)
        cAGTACCACGCGCGCACCACACAACAGCGGCACCCACAGGCGCTCGCTGGCAGCGTCGAAATTGATCGAATAGAAATGCAGCTCGCAATCATCGCTGCGCATGCCGAATGCGGCGATCACCGCCTGGCAGTGCATGGCGAGTTCGCCATg                                                                                                                                            <  2:323277/150‑1 (MQ=255)
         aGTACCACGCGCGCACCACACAACAGCGGCACCCACAGGCGCTCGCTGGCAGCGTCGAAATTGATCGAATAGAAATGCAGCTCGCAATCATCGCTGCGCATGCCGAATGCGGCGATCACCGCCTGGCAGTGCATGGCGAACTCGCCATgg                                                                                                                                           <  1:1143576/150‑1 (MQ=255)
         aGTACCACGCGCGCACCACACAACAGCGGCACCCACAGGCGCTCGCTGGCAGCGTCGAAATTGATCGAATAGAAATGCAGCTCGCAATCATCGCTGCGCATGCCGAATGCGGCGATCACCGCCTGGCAGTGCATGGCGAACTCGCCATgg                                                                                                                                           <  1:91932/150‑1 (MQ=255)
           tACCACGCGCGCACCACACAACAGCGGCACCCACAGGCGCTCGCTGGCAGCGTCGAAATTGATCGAATAGAAATGCAGCTCGCAATCATCGCTGCGCATGCCGAATGCGGCGATCACCGCCTGGCAGTGCATGGCGAACTCGCCATGGCt                                                                                                                                         <  2:219626/150‑1 (MQ=255)
            aCCACGCGCGCACCACACAACAGCGGCACCCACAGGCGCTCGCTGGCAGCGTCGAAATTGATCGAATAGAAATGCAGCTCGCAATCATCGCTGCGCATGCCGAATGCGGCGATCACCGCCTGGCAGTGCATGGCGAGTTCGCCATgcact                                                                                                                                        <  1:354484/150‑5 (MQ=255)
             ccACGCGCGCACCACACAACAGCGGCACCCACAGGCGCTCGCTGGCAGCGTCGAAATTGATCGAATAGAAATGCAGCTCGCAATCATCGCTGCGCATGCCGAATGCGGCGATCACCGCCTGGCAGTGCATGGCGAACTCGCCATGGCTGa                                                                                                                                       >  2:1089126/1‑150 (MQ=255)
              cACGCGCGCACCACACAACAGCGGCACCCACAGGCGCTCGCTGGCAGCGTCGAAATTGATCGAATAGAAATGCAGCTCGCAATCATCGCTGTGCATGCCGAATGCGGCGATCACCGCCTGGCAGTGCATGGCGAACTCGCCATGGCTGac                                                                                                                                      <  2:986116/150‑1 (MQ=255)
              cACGCGCGCACCACACAACAGCGGCACCCACAGGCGCTCGCTGGCAGCGTCGAAATTGATCGAATAGAAATGCAGCTCGCAATCATCGCTGCGCATGCCGAATGCCGCGATCACCGCCTGGCAGTGCATGGCGAACTCGCCATGGCTGac                                                                                                                                      >  2:116891/1‑150 (MQ=255)
                       accacACAACAGCGGCACCCACAGGCGCTCGCTGGCAGCGTCGAAATTGATCGAATAGAAATGCAGCTCGCAATCATCGCTGCGCATGCCGAATGCGGCGATCACCGCCTGGCAGTGCATGGCGAACTCGCCAt                                                                                                                                             >  2:702515/1‑134 (MQ=255)
                       accacACAACAGCGGCACCCACAGGCGCTCGCTGGCAGCGTCGAAATTGATCGAATAGAAATGCAGCTCGCAATCATCGCTGCGCATGCCGAATGCGGCGATCACCGCCTGGCAGTGCATGGCGAACTCGCCAt                                                                                                                                             <  1:702515/134‑1 (MQ=255)
                         cacacaACAGCGGCACCCACAGGCGCTCGCTGGCAGCGTCGAAATTGATCGAATAGAAATGCAGCTCGCAATCATCGCTGCGCATGCCGAAAGCGGCGATCACCGCCTGGCAGTGCATCGCGAACTCGCCATGGCTGCCCACCCCGCCCt                                                                                                                           >  2:687657/1‑150 (MQ=255)
                         cacacaACAGCGGCACCCACAGGCGCTCGCTGGCAGCATCGAAATTGATCGAATAGAAATGCAGCTCGCAATCATCGCTGCGCATGCCGAATGCGGCGATCACCGCCTGGCAGTGCATAGCGAACTCGCCATGGCTGACCACCACGCCCt                                                                                                                           >  2:140431/1‑150 (MQ=255)
                           cacaACAGCGGCACCCACAGGCGCTCGCTGGCAGCGTCGAAATTGATCGAATAGAAATGCAGCTCGCAATCATCGCTGTGCATGCCGAATGCGGCGATCACCGCCTGGCAGTGCATGGCGAACTCGCCATGGCTGACCACCACGCCCTTg                                                                                                                         <  2:1498947/150‑1 (MQ=255)
                           cacaACAGCGGCACCCACAGGCGCTCGCTGGCAGCGTCGAAATTGATCGAATAGAAATGCAGCTCGCAATCATCGCTGCGCATGCCGAATGCGGCGATCACCGCCTGGCAGTGCATGGCGAACTCGCCATgg                                                                                                                                           >  1:1029753/1‑132 (MQ=255)
                           cacaACAGCGGCACCCACAGGCGCTCGCTGGCAGCGTCGAAATTGATCGAATAGAAATGCAGCTCGCAATCATCGCTGCGCATGCCGAATGCGGCGATCACCGCCTGGCAGTGCATGGCGAACTCGCCATgg                                                                                                                                           <  2:1029753/132‑1 (MQ=255)
                           cacaACAGCGGCACCCACAGGCGCTCGCTGGCAGCGTCGAAATTGATCGAATAGAAATGCAGCTCGCAATCATCGCTGCGCATGCCGAATGCGGCGATCACCGCCTGGCAGTGCATGGCGAACTCGCCATGGCTGACCACCACGCCCTTg                                                                                                                         <  1:1830960/150‑1 (MQ=255)
                           cacaACAGCGGCACCCACAGGCGCTCGCTGGCAGCGTCGAAATTGATCGAATAGAAATGCAGCTCGCAATCATCGCTGCGCATGCCGAATGCGGCGATCACCGCCTGGCAGTGCATGGCGAACTCGCCATGGCTGACCACCACGCCCTTg                                                                                                                         >  1:1920255/1‑150 (MQ=255)
                            acaacaGCGGCACCCACAGGCGCTCGCTGGCAGCGTCGAAATTGATCGAATAGAAATGCAGCTCGCAATCATCGCTGCGCATGCCGAATGCGGCGATCACCGCCTGGCAGTGCATGGCGAACTCGCCATGGCTGACCACCACGCCCTTgg                                                                                                                        <  1:1175040/150‑1 (MQ=255)
                            acaacaGCGGCACCCACAGGCGCTCGCTGGCAGCGTCGAAATTGATCGAATAGAAATGCAGCTCGCAATCATCGCTGCGCATGCCGAATGCGGCGATCACCGCCTGGCAGTGCATGGCGAACTCGCCATGGCTGACCACCACGCCCTTgg                                                                                                                        <  2:370136/150‑1 (MQ=255)
                            acaacaGCGGCACCCACAGGCGCTCCATGGCAGCGTCGATATTGTTCGAATAGAAATGCAGCTCGCAATCATCGCTGCGCATGCCGAATGCGGCGATCACCGCCTGGCAGTGCATGGCGAACTCGCCATGGCTGACCACCACGCCCTTgg                                                                                                                        <  2:1260390/150‑1 (MQ=255)
                             caataGCGGCACCCACAGGCGCTCGCTGGCAGCGTCGAAATTGATCGAATAGAAATGCAGCTCGCAATCATCGCTGCGCATGCCGAATGCGGCGATCACCGCCTGGCAGTGCATGGCGAACTCGCCATGGCTGACCACCACGCCCTTggg                                                                                                                       <  1:1494878/150‑1 (MQ=255)
                             caacaGCGGCACCCACAGGCGCTCGCTGGCAGCGTCGAAATTGATCGAATAGAAATGCAGCTCGCAATCATCGCTGCGCATGCCGAATGCGGCGATCACCGCCTGGCAGTCCATGGCGAACTCGCCATGGCTGACCACCACGcccgatcg                                                                                                                       >  1:1841280/1‑145 (MQ=255)
                                caGCGGCACCCACAGGCGCTCGCTGGCAGCGTCGAAATTGATCGAATAGAAATGCAGCTCGCAATCATCGCTGCGCATGCCGAATGCGGCGATCACCGCCTGGCAGTGCATGGCGAACTCGCCATGGCTGACCTCCACGCCCTTGGGttt                                                                                                                    <  2:465270/150‑1 (MQ=255)
                                caGCGGCACCCACAGGCGCTCGCTGGCAGCGTCGAAATTGATCGAATAGAAATGCAGCTCGCAATCATCGCTGCGCATGCCGAATGCGGCGATCACCGCCTGGCAGTGCATGGCGAACTCGCCATGGCTGACCACCACGCCCTTGGGttt                                                                                                                    >  2:728118/1‑150 (MQ=255)
                                       aCCCACAGGCGCTCGCTGGCAGCGTCGAAATTGTTCGAATAGAAATGCAGCTCGCAATCATCGCTGCGCATGCCGAATGCGGCGATCACCGCCTGGCAGTGCATGGCGAACTCGCCATGGCTGACCACCACGCCCTTGGGTTTGCCGGTg                                                                                                             <  1:595670/150‑1 (MQ=255)
                                       aCCCACAGGCGCTCGCTGGCAGCGTCGAAATTGATCGAATAGAAATGCAGCTCGCAATCATCGCTGCGCATGCCGAATGCGGCGATCACCGCCTGGCAGTGCATGGCGAACTCGCCATGGCTGACCACCACGCCCTTGGGTTTGCCGGTg                                                                                                             <  1:1433704/150‑1 (MQ=255)
                                        cccACAGGCGCTCGCTGGCAGCGTCGAAATTGATCGAATAGAAATGCAGCTCGCAATCATCGCTGCGCATGCCGAATGCGGCGATCACCGCCTGGCAGTGCATGGCGAACTCGCCATGGCTGACCACCACGCCCTTGGGTTTGCCGGTgg                                                                                                            <  2:29378/150‑1 (MQ=255)
                                           acaGGCGCTCGCTGGCAGCGTCGAAATTGATCGAATAGAAATGCAGCTCGCAATCATCGCTGCGCATGCCGAATGCGGCGATCACCGCCTGGCAGTGCATGGCGAACTCGCCATGGCTGACCACCACGCCCTTGGGTTTGCCGGTGGAGc                                                                                                         >  1:652638/1‑150 (MQ=255)
                                              ggCGCTCGCTGGCAGCGTCGAAATTGATCGAATAGAAATGCAGCTCGCAATCATCGCTGCGCATGCCGAATGCGGCGATCACCGCCTGGCAGTGCATGGCGAACTCGCCATGGCTGACCACCACGCCCTTGGGTTTGCCGGTGGAGCCTg                                                                                                      <  2:652638/150‑1 (MQ=255)
                                                cgctcgctGGCAGCGTCGAAATTGATCGAATAGAAATGCAGCTCGCAATCATCGCTGCGCATGCCGAATGCGGCGATCACCGCCTGGCAGTGCATGGCGAACTCGCCATGGCTGACCACCACGCCCTTGGGTTTGCCGGTGGAGCCTGAg                                                                                                    <  2:1737349/150‑1 (MQ=255)
                                                cgctcgctGGCAGCGTCGAAATTGATCGAATAGAAATGCAGCTCGCAATCATCGCTGCGCATGCCGAATGCGGCGATCACCGCCTGGCAGTGCATGGCGAACTCGCCATGGCTGACCACCACGCCCTTGGGTTTGCCGGTGGAGCCTGAg                                                                                                    <  2:1429224/150‑1 (MQ=255)
                                                cgctcgctGGCAGCGTCGAAATTGATCGAATAGAAATGCAGCTCGCAATCATCGCTGCGCATGCCGAATGCGGCGATCACCGCCTGGCAGTGCATGGCGAACTCGCCATGGCTGACCACCACGCCCTTGGGTTTGCCGGTGGAGCCTGAg                                                                                                    <  2:742699/150‑1 (MQ=255)
                                                 gctcgctGGCAGCGTCGAAATTGATCGAATAGAAATGCAGCTCGCAATCATCGCTGCGCATGCCGAATGCGGCGATCACCGCCTGGCAGTGCATGGCGAACTCGCCATGGCTGACCACCACGCCCTTGGGTTTGCCGGTGGAGCCTGAgg                                                                                                   >  1:911061/1‑150 (MQ=255)
                                                  ctcgctGGCAGCGTCGAAATTGATCGAATAGAAATGCAGCTCGCAATCATCGCTGCGCATGCCGAATGCGGCGATCACCGCCTGGCAGTGCATGGCGAACTCGCCATGGCTGACCACCACGCCCTTGGGTTTGCCGGTGGAGCCTGAGgt                                                                                                  >  1:605623/1‑150 (MQ=255)
                                                  ctcgctGGCAGCGACGAAATTGATCGAATAGAAATGCAGCTCGCAATCATCGCTGCGCATGCCGAATGCGGCGATCACCGCCTGGCAGTGCATGGCGAACTCGCCATGGCTGACCACCACGCCCTTGGGTTTGCCGGTGGAGCCTGAGgt                                                                                                  <  2:1788928/150‑1 (MQ=255)
                                                   ccgctGGCAGCGTCGAAATTGATCGAATAGCAATGCAGCTCGCAATCATCGCTGCGCATGCCGAATGCGGCGATCACCGCCTGGCAGTGCATGGCGAACTCGCCATGGCTGACCACCACGCCCTTGGGTTTGCCGGTGGAGCCTGAGgtg                                                                                                 <  2:23110/149‑1 (MQ=255)
                                                      ctGGCAGCGTCGAAATTGATCGAATAGAAATGCAGCTCGCAATCATCGCTGCGCATGCCGAATGCGGCGATCACCGCCTGGCAGTGCATGGCGAACTCGCCATGGCTGACCACCACGCCCTTGGGTTTGCCGGTGGAGCCTGAGGTGTAg                                                                                              <  1:742089/150‑1 (MQ=255)
                                                           aGCGTCGAAATTGATCGAATAGAAATGCAGCTCGCAATCATCGCTGCGCATGCCGAATGCGGCGATCACCGCCTGGCAGTGCATGGCGAGTTAGCACCCACGTATCACCACGCCCTTGGGTTTGCCGGTGGAGCCTGAGGTGTAGATCAg                                                                                         <  2:1911080/150‑1 (MQ=255)
                                                              gTCGAAATTGATCGAATAGAAATGCAGCTCGCAATCATCGCTGCGCATGCCGAATGCGGCGATCACCGCCTGGCAGTGCATGGCGAACTCGCCATGGCTGACCACCACGCCCTTGGGTTTGCCGGTGGAGCCTGAGGTGTAGATCAGGTa                                                                                      >  1:1050809/1‑150 (MQ=255)
                                                                cGAAATTGATCGAATAGAAATGCAGCTCGCAATCATCGCTGCGCATGCCGAATGCGGCGATCACCGCCTGGCAGTGCATGGCGAACTCGCCATGGCTGACCACCACGCCCTTGGGTTTGCCGGTGGAGCCTGAGGTGTAGATCAGGTAAg                                                                                    >  2:1566794/1‑150 (MQ=255)
                                                                cGAAATTGATCGAATAGAAATGCAGCTCGCAATCATCGCTGCGCATGCCGAATGCGGCGATCACCGCCTGGCAGTGCATGGCGAACTCGCCATGGCTGACCACCACGCCCTTGGGTTTGCCGGTGGAGCCTGAGGTGTAGATCAGGTAAg                                                                                    <  1:1089126/150‑1 (MQ=255)
                                                                 gAAATTGATCGAATAGAAATGCAGCTCGCAATCATCGCTGCGCATGCCGAATGCGGCGATCACCGCATGGCAGTGCATGGCGAACTCGCCATGGCTGACCACCACGCCCTTGGGTTTGCCGGTGGAGCCTGAGGTGTAGATCAGGTAAGc                                                                                   >  2:381195/1‑150 (MQ=255)
                                                                  aaaTTGATCGAATAGAAATGCAGCTCGCAATCATCGCTGCGCATGCCGACTGCGGCGATCACCGCCTGGCAGTGCATGGCGAACTCGCCATGGCTGACCACCACGCCCTTGGGTTTGCCGGTGGAGCCTGAGGTGTAGATCAGGTAAGcc                                                                                  >  2:214766/1‑150 (MQ=255)
                                                                   aaTTGATCGAATAGAAATGCAGCTCGCAATCATCGCTGCGCATGCCGAATGCGGCGATCACCGCCTGGCAGTGCATGGCGAACTCGCCATGGCTGACCACCACGCCCTTGGGTTTGCCGGTGGCGCCTGAGGTGTAGATCAGGTAAGCCt                                                                                 >  1:1835945/1‑150 (MQ=255)
                                                                    aTTGATCGAATAGAAATGCAGCTCGCAATCATCGCTGCGCATGCCGAATGCGGCGATCACCGCCTGGCAGTGCATGGCGAACTCGCCATGGCTGACCACCACGCCCTTGGGTTTGCCGGTGGAGCCTGAGGTGTAGATCAGGTAAGCCTg                                                                                >  1:649330/1‑150 (MQ=255)
                                                                    aTTGATCGAATAGAAATGCAGCTCGCAATCATCGCTGCGCATGCCGAATGCGGCGATCACCGCCTGGCAGTGCATGGCGAACTCGCCATGGCTGACCACCACGCCCTTGGGTTTGCCGGTGGAGCCTGAGGTGTAGATCAGGTAAGCCTg                                                                                >  1:1276461/1‑150 (MQ=255)
                                                                     ttGATCGAATAGAAATGCAGCTCGCAATCATCGCTGCGCATGCCGAATGCGGCGATCACCGCCTGGCAGTGCATGGCGAACTCGCCATGGCTGACCACCACGCCCTTg                                                                                                                         >  2:1019114/1‑108 (MQ=255)
                                                                     ttGATCGAATAGAAATGCAGCTCGCAATCATCGCTGCGCATGCCGAATGCGGCGATCACCGCCTGGCAGTGCATGGCGAACTCGCCATGGCTGACCACCACGCCCTTg                                                                                                                         <  1:1019114/108‑1 (MQ=255)
                                                                      tGATCGAATAGAAATGCAGCTCGCAATCATCGCTGCGCATGCCGAATGCTGAGAGCACCGCCTGGCAGTGCATGGCGAACTCGCCATGGCTGACCACCACGCCCTTGGGTTTGCCGGTGGAGCCTGAGGTGTAGATCAGGTAAGCCTGGt                                                                              <  1:116891/150‑1 (MQ=255)
                                                                      tGATCGAATAGAAATGCAGCTCGCAATCATCGCTGCGCATGCCGAATGCGGCGATCACCGCCTGGCAGTGCATGGCGAACTCGCCATGGCTGACCACCACGCCCTTGGGTTTGCCGGTGGAGCCTGAGGTGTAGATCAGGTAAGCCTGGt                                                                              <  1:824659/150‑1 (MQ=255)
                                                                       gATCGAATAGAAATGCAGCTCGCAATCATCGCTGCGCATGCCGAATGCGGCGATCACCGCCTGGCAGTGCATGGCGAACTCGCCATGGCTGACCACCACGCCCTTGGGTTTGCCGGTGGAGCCTGAGGTGTAGATCAGGTAAGCCTGGtg                                                                             >  1:1454788/1‑150 (MQ=255)
                                                                           gAATAGAAATGCAGCTCGCAATCATCGCTGCGCATGCCGAATGCGGCGATCACCGCCTGGCAGTGCATGGCGAACTCGCCATGGCTGACCACCACGCCCTTGGGTTTGCCGGTGGAGCCTGAGGTGTAGATCAGGTAAGCCTg                                                                                >  2:1758323/1‑143 (MQ=255)
                                                                           gAATAGAAATGCAGCTCGCAATCATCGCTGCGCATGCCGAATGCGGCGATCACCGCCTGGCAGTGCATGGCGAACTCGCCATGGCTGACCACCACGCCCTTGGGTTTGCCGGTGGAGCCTGAGGTGTAGATCAGGTAAGCCTg                                                                                <  1:1758323/143‑1 (MQ=255)
                                                                           gAATAGAAATGCAGCTCGCAATCATCGCTGCGCATGCCGAATGCGGCGATCACCGCCTGGCAGTGCATGGCGAACTCGCCATGGCTGACCACCACGCCCTTGGGTTTGCCGGTGGAGCCTGAGGTGTAGATCAGGTAAGCCTGGtgctgc                                                                         >  1:565035/1‑150 (MQ=255)
                                                                           gAATAGAAATGCAGCTCGCAATCATCGCTGCGCATGCCGAATGCGGCGATCACCGCCTGGCAGTGCATGGCGAACTCGCCATGGCTGACCACCACGCCCTTGGGTTTGCCGGTGGAGCCTGAGGTGTAGATCAGGTAAGCCTGGtgctgc                                                                         <  1:1917165/150‑1 (MQ=255)
                                                                            aaTAGAAATGCAGCTCGCAATCATCGCTGCGCATGCCGAATGCGGCGATCACCGCCTGGCAGTGCATGGCGAACTCGCCATGGCTGACCACCACGCCCTTGGGTTTGCCGGTGGAGCCTGAg                                                                                                    <  2:69068/122‑1 (MQ=255)
                                                                            aaTAGAAATGCAGCTCGCAATCATCGCTGCGCATGCCGAATGCGGCGATCACCGCCTGGCAGTGCATGGCGAACTCGCCATGGCTGACCACCACGCCCTTGGGTTTGCCGGTGGAGCCTGAg                                                                                                    >  1:69068/1‑122 (MQ=255)
                                                                              tAGAAATGCAGCTCGCAATCATCGCTGCGCATGCCGAATGCGGCGATCACCGCCTGGCAGTGCATGGCGAACTCGCCATGGCTGACCACCACGCCCTTGGGTTTGCCgg                                                                                                               <  2:620689/109‑1 (MQ=255)
                                                                              tAGAAATGCAGCTCGCAATCATCGCTGCGCATGCCGAATGCGGCGATCACCGCCTGGCAGTGCATGGCGAACTCGCCATGGCTGACCACCACGCCCTTGGGTTTGCCgg                                                                                                               >  1:620689/1‑109 (MQ=255)
                                                                                 aaaTGCAGCTCGCAATCATCGCTGCGGAGGTCGATGGCGGCTATCACCGCCTGGCAGTTCAGGGCGAACTCTTCATGTCTGACCACCACGCCCTGGGGTTTGCCGGTGGAGCTTTATGTGTATAGCAGGTAATCCTGGTGCTTCGGCAgt                                                                   <  2:1341617/150‑2 (MQ=255)
                                                                                 aaaTGCAGCTCGCAATCATCGCTGCGCATGCCGAATGCGGCGATCACCGCCTGGCAGTGCATGGCGAACTCGCCATGGCTGACCACCACGCCCTTGGGTTTGCCGGTGGAGCCTGAGGTGTAGATCAGGTAAGCCTGGTGCTGCGGCAgg                                                                   >  2:1822574/1‑150 (MQ=255)
                                                                                 aaaTGCAGCTCGCAATCATCGCTGCGCATGCCGAATGCGGCGATCACCGCCTGGCAGTGCATGGCGAACTCGCCATGGCTGACCACCACGCCCTTGGGTTTGCCGGTGGAGCCTGAGGTGTAGATCAGGTAAGCCTGGTGCTGCGGCAgg                                                                   >  2:446576/1‑150 (MQ=255)
                                                                                  aaTGCAGCTCGCAATCATCGCTGCGCATGCCGAATGCGGCGATCACCGCCTGGCAGTGCATGGCGAACTCGCCATGGCTGACCACCACGCCCTTGGGTTTGCCGGTGGAGCCTGAg                                                                                                    <  1:273009/116‑1 (MQ=255)
                                                                                  aaTGCAGCTCGCAATCATCGCTGCGCATGCCGAATGCGGCGATCACCGCCTGGCAGTGCATGGCGAACTCGCCATGGCTGACCACCACGCCCTTGGGTTTGCCGGTGGAGCCTGAg                                                                                                    >  2:273009/1‑116 (MQ=255)
                                                                                     gCAGCTCGCAATCATCGCTGCGCATGCCGAATGCGGCGATCACCGCCTGGCAGTGCATGGCGAACTCGCCATGGCTGACCACCACGCCCTTGGGTTTGCCGGTGGAGCCTGAGGTGTAGATCAGGTAAGCCTGGTGCTGCGGCAGGCTGa                                                               <  2:246691/150‑1 (MQ=255)
                                                                                      cAGCTCGCAATCATCGCTGCGCATGCCGAATGCGGCGATCACCGCCTGGCAGTGCATGGCGAACTCGCCATGGCTGACCACCACGCCCTTGGGTTTGCCGGTGGAGCCTGAGGTGTAGATCAGGTAAGCCTGGTGCTGCGGCAGGCTGAg                                                              <  2:1490641/150‑1 (MQ=255)
                                                                                      cAGCTCGCAATCATCGCTGCGCATGCCGAATGCGGCGATCACCGCCTGGCAGTGCATGGCGAACTCGCCATGGCTGACCACCACGCCCTTGGGTTTGCCGGTGGAGCCTGAGGTGTAGATCAGGTAAGCCTGGTGCTGCGGCAGGCTGAg                                                              <  2:843877/150‑1 (MQ=255)
                                                                                       aGCTCGCAATCATCGCTGCGCATGCCGAATGCGGCGATCACCGCCTGGCAGTGCATGGCGAACTCGCCATGGCTGACCACCACGCCCTTGGGTTTGCCGGTGGAGc                                                                                                         <  1:994706/106‑1 (MQ=255)
                                                                                       aGCTCGCAATCATCGCTGCGCATGCCGAATGCGGCGATCACCGCCTGGCAGTGCATGGCGAACTCGCCATGGCTGACCACCACGCCCTTGGGTTTGCCGGTGGAGc                                                                                                         >  2:994706/1‑106 (MQ=255)
                                                                                       aGCTCGCAATCATCGCTGCGCATGCCGAATGCGGCGATCACCGCCTGGCAGTGCATGGCGAACTCGCCATGGCTGACCACCACGCCCTTGGGTTTGCCGGTGGAGCCTGAGGTGTAGATCAGGTAAGCCTGGTGCTGCGGCAGGCTGAgg                                                             <  1:214766/150‑1 (MQ=255)
                                                                                         cTCGCAATCATCGCTGCGCATGCCGAATGCGGCGATCACCGCCTGGCAGTGCATGGCGAACTCGCCATGGCTGACCACCACGCCCTTGGGTTTGCCGGTGGAGCCTGAGGTGTAGATCAGGTAAGCCTGGTGCTGCGGCAGGCTGAGGtt                                                           >  2:196892/1‑150 (MQ=255)
                                                                                                   tcGCTGCGCATGCCGAATGCGGCGATCACCGCCTGGCAGTGCATGGCGAACTCGCCATGGCTGACCACCACGCCCTTGGGTTTGCCGGTGGAGCCTGAGGTGTAGATCAGGTAAGCCTGGTGCTGCGGCAGGCTGAGGTTGTCCAGCGGt                                                 <  2:565035/150‑1 (MQ=255)
                                                                                                         cgcNTGCCGAATGCGGCGATCACCGCCTGGCAGTGCATGGCGAACTCGCCATGGCTGACCACCACGCCCTTGGGTTTGCCGGTGGAGCCTGAGGTGTAGATCAGGTAAGCCTGGGGCTGAGGCAGGCTGAGGTTGTCCAGCGGTGCATCg                                           >  2:1195488/1‑150 (MQ=255)
                                                                                                         cgcATGCCGAATGCGGCGATCACCGCCTGGCAGTGCATTGCGAACTCGCCATGGCTGACCACCACGCCCTTGGGTTTGCCGGTGGAGCCTGAGGTGTAGATCAGGTAAGCCTGGTGCTGCGGCAGGCTGAGGTTCTCCAGCGGTGCATCg                                           >  2:758283/1‑150 (MQ=255)
                                                                                                           cATGCCGAATGCGGCGATCACCGCCTGGCAGTGCATGGCGAACTCGCCATGGCTGACCACCACGCCCTTGGGTTTGCCGGTGGAGCCTGAGGTGTAGATCAGGTAAGCCTGGTGCTGCGGCAGGCTGAGGTTGTCCAGCGGTGCATCGCt                                         <  1:1196065/150‑1 (MQ=255)
                                                                                                               ccGAATGCGGCGATCACCGCCTGGCAGTGCATGGCGAACTCGCCATGGCTGACCACCACGCCCTTGGGTTTGCCGGTGGAGCCTGAGGTGTAGATCAGGTAAGCCTGGTGCTGCGGCAGGCTGAGGTTGTCCAGCGGTGCATCGCTGTAg                                     >  1:1471861/1‑150 (MQ=255)
                                                                                                               ccGAATGCGGCGATCACCGCCTGGCAGTGCATGGCGAACTCGCCATGGCTGACCACCACGCCCTTGGGTTTGCCGGTGGAGCCTGAGGTGTAGATCAGGTAAGCCTGGTGCTGCGGCAGGCTGAGGTTGTCCAGCGGTGCATCGCTGTAg                                     <  1:1410319/150‑1 (MQ=255)
                                                                                                                       ggcgATCACCGCCTGGCAGTGCATGGCGAACTCGCCATGGCTGACCACCACGCCCTTGGGTTTGCCGGTGGAGCCTGAGGTGTAGATCAGGTAAGc                                                                                   <  1:1370778/96‑1 (MQ=255)
                                                                                                                       ggcgATCACCGCCTGGCAGTGCATGGCGAACTCGCCATGGCTGACCACCACGCCCTTGGGTTTGCCGGTGGAGCCTGAGGTGTAGATCAGGTAAGc                                                                                   >  2:1370778/1‑96 (MQ=255)
                                                                                                                       ggcgATCACCGCCTGGCAGTGCATGGCGAACTCGCCATGGCTGACCACCACGCCCTTGGGTTTGCCGGTGGAGCCTGAGGTGTAGATCAGGTAAGCCTGGTGCTGCGGCAgg                                                                   >  1:1213802/1‑112 (MQ=255)
                                                                                                                       ggcgATCACCGCCTGGCAGTGCATGGCGAACTCGCCATGGCTGACCACCACGCCCTTGGGTTTGCCGGTGGAGCCTGAGGTGTAGATCAGGTAAGCCTGGTGCTGCGGCAgg                                                                   <  2:1213802/112‑1 (MQ=255)
                                                                                                                           aTCACCGCCTGGCAGTGCATGGCGAACTCGCCATGGCTGACCACCACGCCCTTGGGTTTGCCGGTGGAGCCTGAGGTGTAGATCAGGGAGGCCTGCTGCTCCGGCAGACTGAGGTTGTCCAGCGGTGCATCGCTGTAGGCCGCCAGGCTg                         >  2:28436/1‑150 (MQ=38)
                                                                                                                            tCACCGCCTGGCAGTGCATGGCGAACTCGCCATGGCTGACCACCACGCCCTTGGGTTTGCCGGTGGAGCCTGAGGTGTAGATCAGGTAAGCCTGGTGCTGCGGCAGGCTGAGGTTGTCCAGCGGTGCATCGCTGTAGGCCGCCAGGCTgg                        >  2:1419405/1‑150 (MQ=255)
                                                                                                                              aCCGCCTGGCAGTGCATGGCGAACTCGCCATGGCTGACCACCACGCCCTTGGGTTTGCCGGTGGa                                                                                                           >  2:1207446/1‑65 (MQ=255)
                                                                                                                              aCCGCCTGGCAGTGCATGGCGAACTCGCCATGGCTGACCACCACGCCCTTGGGTTTGCCGGTGGa                                                                                                           <  1:1207446/65‑1 (MQ=255)
                                                                                                                              aCCGCCTGGCAGTGCATGGCGAACTCGCCATGGCTGACCACCACGCCCTTGGGTTTGCCGGTGGAGCCTGAGGTGTAGATCAGGTAAGCCTGGTGCTGCGGCAGGCTGAGGTTGTCCAGCGGTGCATCGCTGTAGGCCGCCAGGCTggcg                      <  2:1630298/150‑1 (MQ=255)
                                                                                                                                cGCCTGGCAGTGCATGGCGAACTCGCCATGGCTGACCACCACGCCCTTGGGTTTGCCGGTGGAGCCTGAGGTGTAGATCAGGTAAGCCTGGTGCTGCGGCAGGCTGAGGTTGTCCAGCGGTGCATCGCTGTAGGCCGCCAGGCTggcggc                    <  1:1566794/150‑1 (MQ=255)
                                                                                                                                   cTGGCAGTGCATGGCGAACTCGCCATGGCTGACCACCACGCCCTTGGGTTTGCCGGTGGAGCCTGAGGTGTAGATCAGGTAAGCCTGGTGCTGCGGCAGGCTGAGGTTGTCCAGCGGTGCATCGCTGTAGGCCGCCAGCCTggcggccta                 <  1:446576/150‑4 (MQ=255)
                                                                                                                                   cTGGCAGTGCATGGCGAACTCGCCATGGCTGACCACCACGCCCTTGGGTTTGCCGGTGGAGCCTGAGGTGTAGATCAGGTAAGCCTGGTGCTGCGGCAGGCTGAGATTGTGCATCGGTGCGTCGCTGTAGGCCGCCAGGCGGGCGGCATc                 <  2:1454788/150‑1 (MQ=38)
                                                                                                                                      gCAGTGCATGGCGAACTCGCCATGGCTGACCACCACGCCCTTGGGTTTGCCGGTGGAGCCTGAGGTGTAGATCAGGTAAGCCTGGTGCTGCGGCAGGCTGAGGTTGTCCAGCGGTGCATCGCTGTAGGCCGCCAGGCTGGCGGCATCGTc              >  2:885293/1‑150 (MQ=255)
                                                                                                                                          tGCATGGCGAACTCGCCATGGCTGACCACCACGCCCTTGGGTTTGCCGGTGGAGCCTGAGGTGTAGATCAGGTAAGCCTGGTGCTGCGGCAGGCTGAGGtt                                                           >  1:1334983/1‑101 (MQ=255)
                                                                                                                                          tGCATGGCGAACTCGCCATGGCTGACCACCACGCCCTTGGGTTTGCCGGTGGAGCCTGAGGTGTAGATCAGGTAAGCCTGGTGCTGCGGCAGGCTGAGGtt                                                           <  2:1334983/101‑1 (MQ=255)
                                                                                                                                          tGCATGGCGAACTCGCCATGGCTGACCACCACGCCCTTGGGTTTGCCGGTGGAGCCTGAGGTGTAGATCAGGTAAGCCTGGTGCTGCGGCAGGCTGAGGTTGTCCAGCGGTGCATCGCTGTAGGCCGCCAGGCTGGCGGCATCGTCTTcc          >  2:1089837/1‑150 (MQ=255)
                                                                                                                                           ccATGGCGAGTTCGCCATGGCTGACCACCACGCCCTTGGGTTTGCCGGTGGAGCCTGAGGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAATGTCCAGCGGTGCATCGCTGTAGGCCGCCAGGCTGGCGGCATCGTCTTCCa         >  2:672985/2‑150 (MQ=12)
                                                                                                                                           ccATGGCGAGTTCGCCATGGCTGACCACCACGCCCTTGGGTTTGCCGGTGGAGCCTGAGGTGTAGATCAGGTAAGCCTGGTGCTGCGGCAGGCTGAGGTTGTCCAGCGGTGCATCGCTGTa                                      >  2:648467/2‑121 (MQ=255)
                                                                                                                                           ccATGGCGAGTTCGCCATGGCTGACCACCACGCCCTTGGGTTTGCCGGTGGAGCCTGAGGTGTAGATCAGGTAAGCCTGGTGCTGCGGCAGGCTGAGGTTGTCCAGCGGTGCATCGCTGTa                                      <  1:648467/120‑1 (MQ=255)
                                                                                                                                            cATGGCGAACTCGCCATGGCTGACCACCACGCCCTTGGGTTTGCCGGTGGAGCCTGAGGTGTAGATCAGGTAAGCCTGGTGCTGCGGCAGGCTGAGGTTGTCCAGCGGTGCATCGCTGTAGGCCGCCAGGCTGGCGGCATCGTCTTCCAg        >  2:321843/1‑150 (MQ=255)
                                                                                                                                            cATGGCGAACTCGCCATGGCTGACCACCACGCCCTTGGGTTTGCCGGTGGAGCCTGAGGTGTAGATCAGGTAAGCCTGGTGCTGCGGCAAGCTGAGGTTGTCCAGCGGTGCATCGCTGTAGGCCGCCAGGCTGGCGGCATCGTCTTCCAg        >  2:1125472/1‑150 (MQ=255)
                                                                                                                                                  gAACTCGCCATGGCTGACCACCACGCCCTTGGGTTTGCCGGTGGAGCCTGAGGTGTAGATCAGGTAAGCCTGGTGCTGCGGCAGGCTGAGGTTGTCCAGCGGTGCATCGCTGTAGGCCGCCAGGCTGGCGGCATCGTCTTCCAGGCTCCa  <  1:1094798/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                  
TGGGCGCGCAGTACCACGCGCGCACCACACAACAGCGGCACCCACAGGCGCTCGCTGGCAGCGTCGAAATTGATCGAATAGAAATGCAGCTCGCAATCATCGCTGCGCATGCCGAATGCGGCGATCACCGCCTGGCAGTGCATGGCGAACTCGCCATGGCTGACCACCACGCCCTTGGGTTTGCCGGTGGAGCCTGAGGTGTAGATCAGGTAAGCCTGGTGCTGCGGCAGGCTGAGGTTGTCCAGCGGTGCATCGCTGTAGGCCGCCAGGCTGGCGGCATCGTCTTCCAGGCTCCA  >  NC_002947/4824048‑4824343

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑