| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 4,889,631 | C→A | 7.5% | intergenic (+32/‑121) | hyi → / → glxR | hydroxypyruvate isomerase/2‑hydroxy‑3‑oxopropionate reductase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 4,889,631 | 0 | C | A | 7.5% | 149.4 / 2.1 | 53 | intergenic (+32/‑121) | hyi/glxR | hydroxypyruvate isomerase/2‑hydroxy‑3‑oxopropionate reductase |
| Reads supporting (aligned to +/- strand): ref base C (24/25); new base A (2/2); total (26/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.79e-01 | |||||||||||
CTTCCTGTTCGAACACCTGGACCGCATTGGCTACCAGGGCTGGGTGGGCGCGGAGTACAAGCCGCTGACCACCACCGAAGCGGGCCTGGGCTGGCTGAAAACCCACAACGCAATCTGAGGAACAACTCGGGACCCTGTGGGAGCGGGCTCGCCCG‑CGAACACCGGCAAAGCCGGTGCCATCCACCGAGTTGTCTGCTTCGCGGGCGAGCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCA > NC_002947/4889482‑4889770 | tgaacaggctgtgacctgccttttgcaggttctgagccatgggcttgcccatgatgccggtgccgatgaaaccgattttagccatgagaaattacctctttgtaattgtgatatctcaagcaagctttggccCCCTGTGGGAGCGGGCTc > 2:952345‑M1/133‑150 (MQ=255)tgaacaggctgtgacctgccttttgcaggttctgagccatgggcttgcccatgatgccggtgccgatgaaaccgattttagccatgagaaattacctctttgtaattgtgatatctcaagcaagctttggccCCCTGTGGGAGCGGGCTc > 2:70620‑M1/133‑150 (MQ=255)cTTCCTGTTCGAACACCTGGACCGCATTGGCTACCAGGGCTGGGTGGGCGCGGAGTACAAGCCGCTGACCACCACCGAAGCGGGCCTGGGCTGGCTGAAAACCCACAACGCAATCTGAGGAACAACTCGGGACCCTGTGGGAGCGGGCTc > 2:47817/1‑150 (MQ=255) cTGTTCGAACACCTGGACCGCATTGGCTACCAGGGCTGGGTGGGCGCGGAGTACAAGCCGCTGACCACCACCGAAGCGGGCCTGGGCTGGCTGAAAACCCACAACGCAATCTGAGGAACAACTCGGGACCCTGTGGGAGCGGGCTCGccc > 1:1247206/1‑150 (MQ=255) aggctgtgacctgccttttgcaggttctgagccatgggcttgcccatgatgccggtgccgatgaaaccgattttagccatgagaaattacctctttgtaattgtgatatctcaagcaagctttggccCCCTGTGGGAGCGGGCTCGCCcg > 1:1247529‑M1/128‑150 (MQ=255) aggctgtgacctgccttttgcaggttctgagccatgggcttgcccatgatgccggtgccgatgaaaccgattttagccatgagaaattacctctttgtaattgtgatatctcaagcaagctttggccCCCTGTGGGAGCGGGCTCGCCcg < 2:1085327‑M1/23‑1 (MQ=255) ttCGAACACCTGGACCGCATTGGCTACCAGGGCTGGGTGGGCGCGGAGTACAAGCCGCTGACCACCACCGAAGCGGGCCTGGGCTGGCTGAAAACCCACAACGCAATCTGAGGAACAACTCGGGACCCTGTGGGAGCGGGCTCGCCcg‑cg > 2:841803/1‑150 (MQ=255) ttCGAACACCTGGACCGCATTGGCTACCAGGGCTGGGTGGGCGCGGAGTACAAGCCGCTGACCACCACCGAAGCGGGCCTGGGCTGGCTGAAAACCCACAACGCAATCTGAGGAACAACTCGGGACCCTGTGGGAGCGGGCTCGCCcg‑cg > 2:1597474/1‑150 (MQ=255) ctgtgacctgccttttgcaggttctgagccatgggcttgcccatgatgccggtgccgatgaaaccgattttagccatgagaaattacctctttgtaattgtgatatctcaagcaagctttggccCCCTGTGGGAGCGGGCTCGCCCG‑CGa < 1:1325353‑M1/26‑1 (MQ=255) tgtgacctgccttttgcaggttctgagccatgggcttgcccatgatgccggtgccgatgaaaccgattttagccatgagaaattacctctttgtaattgtgatatctcaagcaagctttggccCCCTGTGGGAGCGGGCTCGCCCG‑CGaa < 1:317215‑M1/27‑1 (MQ=255) gAACACCTGGACCGCATTGGCTACCAGGGCTGGGTGGGCGCGGAGTACAAGCCGCTGACCACCACCGAAGCGGGCCTGGGCTGGCTGAAAACCCACAACGCAATCTGAGGAACAACTCGGGACCCTGTGGGAGCGGGCTCGCCCG‑CGAac > 2:1623757/1‑150 (MQ=255) aaCACCTGGACCGCATTGGCTACCAGGGCTGGGTGGGCGCGGAGTACAAGCCGCTGACCACCACCGAAGCGGGCCTGGGCTGGCTGAAAACCCACAACGCAATCTGAGGAACAACTCGGGACCCTGTGGGAGCGGGCTCGCCCG‑CGAaca > 1:543334/1‑150 (MQ=255) aaCACCTGGACCGCATTGGCTACCAGGGCTGGGTGGGCGCGGAGTACAAGCCGCTGACCACCACCGAAGCGGGCCTGGGCTGGCTGAAAACCCACAACGCAATCTGAGGAACAACTCGGGACCCTGTGGGAGCGGGCGAGCCcg‑ctcc > 2:1787121/1‑145 (MQ=255) aaCACCTGGACCGCATTGGCTACCAGGGCTGGGTGGGCGCGGAGTACAAGCCGCTGACCACCACCGAAGCGGGCCTGGGCTGGCTGAAAACCCACAACGCAATCTGAGGAACAACTCGGGACCCTGTGGGAGCGGGCGAGCCcg‑ctcc < 1:1787121/148‑4 (MQ=255) ccTGGACCGCATTGGCTACCAGGGCTGGGTGGGCGCGGAGTACAAGCCGCTGACCACCACCGAAGCGGGCCTGGGCTGGCTGAAAACCCACAACGCAATCTGAGGAACAACTCGGGACCCTGTGGGAGCGGGCTCGCCCG‑CGAACACCgg < 1:1597474/150‑1 (MQ=255) ccTGGACCGCATTGGCTACCAGGGCTGGGTGGGCGCGGAGTACAAGCCGCTGACCACCACCGAAGCGGGCCTGGGCTGGCTGAAAACCCACAACGCAATCTGAGGAACAACTCGGGACCCTGTGGGAGCGGGCTCGCCCG‑CGAACACCgg < 2:83147/150‑1 (MQ=255) ggACCGCATTGGCTACCAGGGCTGGGTGGGCGCGGAGTACAAGCCGCTGACCACCACCGAAGCGGGCCTGGGCTGGCTGAAAACCCACAACGCAATCTGAGGAACAACTCGGGACCCTGTGGGAGCGGGCTc < 2:225462/132‑1 (MQ=255) ggACCGCATTGGCTACCAGGGCTGGGTGGGCGCGGAGTACAAGCCGCTGACCACCACCGAAGCGGGCCTGGGCTGGCTGAAAACCCACAACGCAATCTGAGGAACAACTCGGGACCCTGTGGGAGCGGGCTc > 1:225462/1‑132 (MQ=255) cGCATTGGCTACCAGGGCTGGGTGGGCGCGGAGTACAAGCCGCTGACCACCACCGAAGCGGGCCTGGGCTGGCTGAAAACCCACAACGCAATCTGAGGAACAACTCGGGACCCTGTGGGAGCGGGCTCGCCCG‑CGAACACCGGCAAAGcc > 2:220652/1‑150 (MQ=255) cGCATTGGCTACCAGGGCTGGGTGGGCGCGGAGTACAAGCCGCTGACCACCACCGAAGCGGGCCTGGGCTGGCTGAAAACCCACAACGCAATCTGAGGAACAACTCGGGACCCTGTGGGAGCGGGCTCGCCCG‑CGAACACCGGCAAAGcc > 1:1159326/1‑150 (MQ=255) tACNAGGGCTGGGTGGGCGCGGAGTACAAGCCGCTGACCACCACCGAAGCGGGCCTGGGCTGGCTGAAAACCCACAACGCAATCTGAGGAACAACTCGGGACCCTGTGGGAGCGGGCAACTCCC‑CCAACACAGGCAAAGCCGGTGCCATc > 2:1185632/1‑150 (MQ=255) tACCAGGGCTGGGTGGGCGCGGAGTACAAGCCGCTGACCACCACCGAAGCGGGCCTGGGCTGGCTGAAAACCCACAACGCAATCTGAGGAACAACTCGGGACCCTGTGGGAGCGGGCTCGCCCG‑CGAACACCGGCAAAGCCGGTGCCATc > 2:60012/1‑150 (MQ=255) catgggcttgcccatgatgccggtgccgatgaaaccgattttagccatgagaaattacctctttgtaattgtgatatctcaagcaagctttggccCCCTGTGGGAGCGGGCTCGCCCG‑CGAACACCGGCAAAGCCGGTGCCATCCACCGa < 2:245585‑M1/55‑1 (MQ=255) gCTGGGTGGGCGCGGAGTACAAGCCGCTGACCACCACCGAAGCGGGCCTGGGCTGGCTGAAAACCCACAAAGCAATCTGAGGAACAACTCGGGACCCTGTGGGAGCGGGCTCGCCCG‑CGAACACCGGCAAAGCCGGTGCCATCCACCGAg < 2:543334/150‑1 (MQ=255) cTGGGTGGGCGCGGAGTACAAGCCGCTGACCACCACCGAAGCGGGCCTGGGCTGGCTGAAAACCCACAACGCAATCGGAGGAACAACTCGGGACCCTGTGGGAGCGGGCTCGCCCG‑CGAACACCGGCAAAGCCGGTGCCATCCACCGAGt < 1:717054/150‑1 (MQ=255) tgggCGCGGAGTACAAGCCGCTGACCACCACCGAAGCGGGCCTGGGCTGGCTGAAAACCCACAACGCAATCTGAGGAACAACTCGGGACCCTGTGGGAGCGGGCTCGCCCG‑CGAACACCGGCAAAGCCGGTGCCATCCACCGAGTTGTCt < 1:60012/150‑1 (MQ=255) tgatgccggtgccgatgaaaccgattttagccatgagaaattacctctttgtaattgtgatatctcaagcaagctttggccCCCTGTGGGAGCGGGCTCGCCCG‑CGAACACCGGCAAAGCCGGTGCCATCCACCGAGTTGTCTGCTTcgc > 1:260889‑M1/82‑150 (MQ=255) atgccggtgccgatgaaaccgattttagccatgagaaattacctctttttaattgtgatatctcaagcaagcttttgccCCCTGTGGGAGCCGGCTCCCCCG‑CGAACCCCGGCCAAGCCGCTCCCCTCCACCGAGTTGTCCgcttctctg > 2:25139‑M1/80‑146 (MQ=255) atgccggtgccgatgaaaccgattttagccatgagaaattacctctttgtaattgtgatatctcaagcaagctttggccCCCTGTGGGAGCGGGCTCGCCCG‑CGAACACCGGCAAAGCCGGTGCCATCCACCGAGTTGTCTGCTTCGCgg > 1:1415111‑M1/80‑150 (MQ=255) atgccggtgccgatgaaaccgattttagccatgagaaattacctctttgtaattgtgatatctcaagcaagctttggccCCCTGTGGGAGCGGGCTCGCCCG‑CGAACACCGGCAAAGCCGGTGCCATCCACCGAGTTGTCTGCTTCGCgg > 1:245585‑M1/80‑150 (MQ=255) aGTACAAGCCGCTGACCACCACCGAAGCGGGCCTGGGCTGGCTGAAAACCCACAACGCAATCTGAGGAACAACTCGGGACCCTGTGGGAGCGGGCTCGCCCG‑CGAACACCGGCAAAGCCGGTGCCATCCACCGAGTTGTCTGCTTCGCgg < 1:617738/150‑1 (MQ=255) aGTACAAGCCGCTGACCACCACCGAAGCGGGCCTGGGCTGGCTGAAAACCCACAACGCAATCTGAGGAACAACTCGGGACCCTGTGGGAGCGGGCTCGCCCG‑CGAACACCGGCAAAGCCGGTGCCATCCACCGAGTTGTCTGCTTCGCgg < 2:1587957/150‑1 (MQ=255) gTACAAGCCGCTGACCACCACCGAAGCGGGCCTGGGCTGGCTGAAAACCCACAACGCAATCTGAGGAACAACTCGGGACCCTGTGGGAGCGGGCTCGCCCG‑CGAACACCGGCAAAGCCGGTGCCATCCACCGAGTTGTCTGCTTCGCggg < 1:52159/150‑1 (MQ=255) cAAGCCGCTGACCACCACCGAAGCGGGCCTGGGCTGGCTGAAAACCCACAACGCAATCTGAGGAACAACTCGGGACCCTGTGGGAGCGGGCTCGCCCG‑CGAACACCGGCAAAGCCGGTGCCATCCACCGAGTTGTCTGCTTCGCGGGCGa > 1:1769332/1‑150 (MQ=255) tGACCACCACCGAAGCGGGCCTGGGCTGGCTGAAAACCCACAACGCAATCTGAGGAACAACTCGGGACCCTGTGGGAGCGGGCTCGCCCG‑CGAACACCGGCAAAGCCGGTGCCATCCACCGAGTTGTCTGCTTCGCGGGCGAGCCCGCTc < 1:370252/150‑1 (MQ=255) accaccaccGAAGCGGGCCTGGGCTGGCTGAAAACCCACAACGCAATCTGAGGAACAACTCGGGACCCTGTGGGAGCGGGCTCGCCCG‑CGAACACCGGCAAAGCCGGTGCCATCCACCGAGTTGTCTGCTTCGCGGGCGAGCCCGCTccc < 2:1769332/150‑1 (MQ=255) ccaccaccGAAGCGGGCCTGGGCTGGCTGAAAACCCACAACGCAATCTGAGGAACGACTCGGGACCCTGTGGGAGCGGGCTCGCCCG‑CGAACACCGGCAAAGCCGGTGCCATCCACCGAGTTGTCTGCTTCGCGGGCGAGCCCGCTCCca < 1:1623757/150‑1 (MQ=255) ccaccaccGAAGCGGGCCTGGGCTGGCTGAAAACCCACAACGCAATCTGAGGAACAACTCGGGACCCTGTGGGAGCGGGCTCGCCCG‑CGAACACCGGCAAAGCCGGTGCCATCCACCGAGTTGTCTGCTTCGCGGGCGAGCCCGCTCCca < 1:183999/150‑1 (MQ=255) caccaccGAAGCGGGCCTGGGCTGGCTGAAAACCCACAACGCAATCTGAGGAACAACTCGGGACCCTGTGGGAGCGGGCTCGCCCG‑CGAACACCGGCAAAGCCGGTGCCATCCACCGAGTTGTCTGCTTCGCGGGCGAGCCCGCTCCcac > 2:104606/1‑150 (MQ=255) ccGAAGCGGGCCTGGGCTGGCTGAAAACCCACAACGCAATCTGAGGAACAACTCGGGACCCTGTGGGAGCGGGCTCGCCCG‑CGAACACCGGCAAAGCCGGTGCCATCCAGGGATTGGTCTGCTTCGCGGGCGAGCCCGCTCCCACAgggg > 1:1534846/1‑150 (MQ=255) ggCCTGGGCTGGCTGAAAACCCACAACGCAATCTGAGGAACAACTCGGGACCCTGTGGGAGCGGGCTCGCCCG‑CGAACACCGGCAAAGCCGGTGCCATCCACCGCGTTGTCTGCTTCGCGGGCGAGCCCGCTCCCACAGGGGGCCAAAgc < 1:903465/150‑1 (MQ=255) tggctgAAAACCCACAACGCAATCTGAGGAACAACTCGGGACCCTGTGGGCGAGGGCTCGCCCG‑CGAACACCGGCAAAGCCGTTGCCATCCACCGAGTTGTCTGCTTCGCGGGCGAGCCCGCTCCCACAGGGGGCCAAAGCTTGCTTgag < 2:1159326/150‑1 (MQ=255) ctctttgtaattgtgatatctcaagcaagctttggccCCCTGTGGGAGCGGGCTCGCCCG‑CGAACACCGGCAAAGCCGGTGCCATCCACCGAGTTGTCTGCTTCGCgg > 2:13991‑M1/38‑108 (MQ=255) ctctttgtaattgtgatatctcaagcaagctttggccCCCTGTGGGAGCGGGCTCGCCCG‑CGAACACCGGCAAAGCCGGTGCCATCCACCGAGTTGTCTGCTTCGCgg < 1:13991‑M1/71‑1 (MQ=255) ccACAACGCAATCTGAGGAACAACTCGGGACCCTGTGGGAGCGGGCTCGccc < 2:1289724/52‑1 (MQ=255) ccACAACGCAATCTGAGGAACAACTCGGGACCCTGTGGGAGCGGGCTCGccc > 1:1289724/1‑52 (MQ=255) ccACAACGCAATCTGAGGAACAACTCGGGACCCTGTGGGAGCGGGCTCGCCCG‑CGAACACCGGCAAAGCCGGTGCCATCCACCGAGTTGTCTGCTTCGCGGGCGAGCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAAtt > 2:1661997/1‑150 (MQ=255) aCGCAATCTGAGGAACAACTCGGGACCCTGTGGGAGCGGGCTCGCCCG‑CGAACACCGGCAAAGCCGGTGCCATCCACCGAGTTGTCTGCTTCGCGGGCGAGCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACaaa < 1:1661997/150‑1 (MQ=255) aaTCTGAGGAACAACTCGGGACCCTGTGGGAGAGGGATCGCCCGTCACATAGCATC‑GATCTGGAGCTAGCCACTCAGAGGTCTGCTTCGCGGGCGAGCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAgg > 1:513578/1‑150 (MQ=255) tGAGGAACAACTCGGGACCCTGTGGGAGCGGGCTCGCCCG‑CGAACACCGGCAAAGCCGGTGCCATCCACCGAGTTGTCTGCTTCGCGGGCGAGCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAAt < 2:1863225/150‑1 (MQ=255) aGGAACAACTCGGGACCCTGTGGGAGCGGGCCCGCACG‑CGAAGACCGGCAAAGCCCGGGCCATCCCCCGACTGGTCTGCTTCGCGGGCGAGCCCGCTCCCACAGGGCGCCACAACTTGCTTGAGATCTCACCATTACAAAGCGGTAAatt > 2:656626/1‑150 (MQ=255) caacTCGGGACCCTATGGGAGCGGGCTCGCCCG‑CGAACACCGGCAAAGCCGGTGCCATCCACCGAGTTGTCTGCTTcgcg < 2:1901488/80‑1 (MQ=255) caacTCGGGACCCTATGGGAGCGGGCTCGCCCG‑CGAACACCGGCAAAGCCGGTGCCATCCACCGAGTTGTCTGCTTcgcg > 1:1901488/1‑80 (MQ=255) tCGGGACCCTGTGGGAGCGGGCTCGCCCG‑CGAACACCGGCAAAGCCGGTGCCATCCACCGAGTTGTCTGCTTCGCGGGCGAGCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCt > 2:596298/1‑150 (MQ=255) ggtgttcGCGGGCGAGCCCG‑CGAACACCGGCAAAGCCGGTGCCATCCACCGAGTTGTCTGCTTCGCGGGCGAGCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGt < 1:1640684‑M1/143‑1 (MQ=255) gGTGGGAGCGGGCTCGCCCG‑CGAACACCGGCAAAGCCGGTGCCATCCACCGAGTTGTCTGCTTCGCGGGCGAGCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGt > 2:1247206‑M1/2‑150 (MQ=255) TGGGAGCGGGCTCGCCCG‑CGAACACCGGCAAAGCCGGTGCCATCCACCGAGTTGTCTGCTTCGCGGGCGAGCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGttt < 2:317215‑M1/150‑1 (MQ=255) GGAGCGGGCTCGCCCG‑CGAACACCGGCAAAGCCGGTGCCATCCACCGAGTTGTCTGCTTCGCGGGCGAGCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCa < 1:1085327‑M1/150‑1 (MQ=255) | CTTCCTGTTCGAACACCTGGACCGCATTGGCTACCAGGGCTGGGTGGGCGCGGAGTACAAGCCGCTGACCACCACCGAAGCGGGCCTGGGCTGGCTGAAAACCCACAACGCAATCTGAGGAACAACTCGGGACCCTGTGGGAGCGGGCTCGCCCG‑CGAACACCGGCAAAGCCGGTGCCATCCACCGAGTTGTCTGCTTCGCGGGCGAGCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCA > NC_002947/4889482‑4889770 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |