Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_002947	1,844,126	Δ1 bp	coding (710/2754 nt)	gacS ←	sensor protein GacS

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,844,125	0	G	.	100.0%	175.1 / NA	39	coding (711/2754 nt)	gacS	sensor protein GacS
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base . (16/23);  total (16/23)

ACTTGATGCGGCTGGCCTCAAGTGCCTCTTTGCGCGCCATGTCCAGCTCGATGTTCTGGATTTCGATGGTTTCCAGGTTCTGGCGCACGTCTTCGGTGGCCTGGTCGATGCTGTGTTGCAGTTCTTCGTGGGCATTCTGCAGGGTTTCGGCCATGCGGTTGATGCCGTGGGCCAGTTCGTCCAGCTCATGGCTGCCCATGGTCGGCAGGCGTTCTTCGAGGTGGCCGTCCTTGAGCTGATTGACCGCGTGCTTGATGCGTTCGATGGGGTCGTTGATGGTGC  >  NC_002947/1843977‑1844258                                                                                                                                                     |                                                                                                                                      aCTTGATGCGGCTGGCCTCAAGTGCCTCTTTGCGCGCCATGTCCAGCTCGATGTTCTGGATTTCGATGGTTTCCAGGTTCTGGCGCACGTCTTCGGTGGCCTGGTCGATGCTGTGTTGCAGTTCTTCGTGGGCATTCTGCAGGGTTTCgc                                                                                                                                      >  2:946260/1‑149 (MQ=255)          ggCTTGCCTCAAGTGCCTCTTTTCGCGCCATGTCCAGCTCGATGTTCTGGATTTCGATTGTTTCCAGGTTCTTGCGCACGTCTTCGGTCGCCTGGTCGATGCTGTGTTGCAGTTCTTCGTGGGCATTCTGCAGGGTTTC‑GCCATGCGGtt                                                                                                                            <  1:704891/150‑1 (MQ=255)                 cTCAAGTGCCTCTTTGCGCGCCATGTCCAGCTCGATGTTCTGGATTTCGATGGTTTCCAGGTTCTGGCGCACGTAATCGCTTGCCTGGTCGATGCTGTGTTGCAGTTCTTCGTGGGCATTCTGCAGGGTTTC‑GCCATGCGGTTGATGCCg                                                                                                                     >  1:445427/1‑150 (MQ=255)                    aaGTGCCTCTTTGCGCGCCATGTCCAGCTCGATGTTCTGGATTTCGATGGTTTCCAGGTTCTGGCGCACGTCTTCGGTGGCCTGGTCGATGCTGTGTTGCAGTTCTTCGTGGGCATTCTGCAGGGTTTC‑GCCATGCGGTTGATGCCGTgg                                                                                                                  <  2:936580/150‑1 (MQ=255)                    aaGTGCCTCTTTGCGCGCCATGTCCAGCTCGATGTTCTGGATTTCGATGGTTTCCAGGTTCTGGCGCACGTCTTCGGTGGCCTGGTCGATGCTGTGTTGCAGTTCTTCGTGGGCATTCTGCAGGGTTTC‑GCCATGCGGTTGATGCCGTgg                                                                                                                  <  2:580189/150‑1 (MQ=255)                       tGCCTCTTTGCGCGCCATGTCCAGCTCGATGTTCTGGATTTCGATGGTTTCCAGGTTCTGGCGCACGTCTTCGGTGGCCTGGTCGATGCTGTGTTGCAGTTCTTCGTGGGCATTCTGCAGGGTTTC‑GCCATGCGGTTGATGCCGTGGGcc                                                                                                               >  1:732453/1‑150 (MQ=255)                          ctctTTGCGCGCCATGTCCAGCTCGATGTTCTGGATTTCGATGGTTTCCAGGTTCTGGCGCACGTCTTCGGTGGCCTGGTCGATGCTGTGTTGCAGTTCTTCGTGGGCATTCTGCAGGGTTTC‑GCCATGCGGTTGATGCCGTGGGCCAGt                                                                                                            <  2:749985/150‑1 (MQ=255)                            ctTTGCGCGCCATGTCCAGCTCGATGTTCTGGATTTCGATGGTTTCCAGGTTCTGGCGCACGTCTTCGGTGGCCTGGTCGATGCTGTGTTGCAGTTCTTCGTGGGCATTCTGCAGGGTTTC‑GCCATGCGGTTGATGCCGTGGGCCAGTTc                                                                                                          <  1:295579/150‑1 (MQ=255)                            ctTTGCGCGCCATGTCCAGCTCGATGTTCTGGATTTCGATGGTTTCCAGGTTCTGGCGCACGTCTTCGGTGGCCTGGTCGATGCTGTGTTGCAGTTCTTCGTGGGCATTCTGCAGGGTTTC‑GCCATGCGGTTGATGCCGTGGGCCAGTTc                                                                                                          <  1:423646/150‑1 (MQ=255)                                        tGTCCAGCTCGATGTTCTGGATTTCGATGGTTTCCAGGTTCTGGCGCACGTCTTCGGTGGCCTGGTCGATGCTGTGTTGCAGTTCTTCGTGGGCATTCTGCAGGGTTTC‑GCCATGCGGTTGATGCCGTGGGCCAGTTCGTCCAGCTCATg                                                                                              <  1:430220/150‑1 (MQ=255)                                                  gATGTTCTGGATTTCGATGGTTTCCAGGTTCTGGCGCACGTCTTCGGTGGCCTGGTCGATGCTGTGTTGCAGTTCTTCGTGGGCATTCTGCAGGGTTTC‑GCCATGCGGTTGATGCCGTGGGCCAGTTCGTCCAGCTCATGGCTGCCCATg                                                                                    >  1:858963/1‑150 (MQ=255)                                                   aTGTTCTGGATTTCGATGGTTTCCAGGTTCTGGCGCACGTCTTCGGTGGCCTGGTCGATGCTGTGTTGCAGTTCTTCGTGGGCATTCTGCAGGGTTTC‑GCCATGCGGTTGATGCCGTGGGCCCGTTCGTCCAGCTCATGGCTGCCCATgg                                                                                   >  1:343258/1‑150 (MQ=255)                                                   aTGTTCTGGATTTCGATGGTTTCCAGGTTCTGGCGCACGTCTTCGGTGGCCTGGTCGATGCTGTGTTGCAGTTCTTCGTGGGCATTCTGCAGGGTTTC‑GCCATGCGGTTGATGCAGTGGGCCAGTTCGTCCAGCTCATGGCTGCCCATgg                                                                                   <  1:946260/150‑1 (MQ=255)                                                            aTTTCGATGGTTTCCAGGTTCTGGCGCACGTCTTCGGTGGCCTGGTCGATGCTGTGTTGCAGTTCTTCGTGGGCATTCTGCAGGGTTTC‑GCCATGCGGTTGATGCCGTGGGCCAGTTCGTCCAGCTCATGGCTGCCCATGGTCGGCAGGc                                                                          >  1:655744/1‑150 (MQ=255)                                                            aTTTCGATGGTTTCCAGGTTCTGGCGCACGTCTTCGGTGGCCTGGTCGATGCTGTGTTGCAGTTCTTCGTGGGCATTCTGCAGGGTTTC‑GCCATGCGGTTGATGCCGTGGGCCAGTTCGTCCAGCTCATGGCTGCCCATGGTCGGCAGGc                                                                          <  2:956133/150‑1 (MQ=255)                                                                 gATGGTTTCCAGGTTCTGGCGCACGTCTTCGGTGGCCTGGTCGATGCTGTGTTGCAGTTCTTCGTGGGCATTCTGCAGGGTTTC‑GCCATGCGGTTGATGCCGTGGTCCAGTTCGTCCAGCTCCTCGGTGCCCATGGTCGGCAGGCGttct                                                                     >  2:527330/1‑150 (MQ=255)                                                                      tttCCAGGTTCTGGCGCACGTCTTCGGTGGCCTGGTCGATGCTGTGTTGCAGTTCTTCGTGGGCATTCTGCAGGGTTTC‑GCCATGCGGTTGATGCCGTGGGCCAGTTCGTCCAGCTCATGGCTGCCCATGTTCGTCAGGCGTTCTTCGAg                                                                <  2:858963/150‑1 (MQ=255)                                                                      tttCCAGGTTCTGGCGCACGTCTTCGGTGGCCTGGTCGATGCTGTGTTGCAGTTCTTCGTGGGCATTCTGCAGGGTTTC‑GCCATGCGGTTGATGCCGTGGGCCAGTTCGTCCAGCTCATGGCTGCCCATGGTCGGCAGGCGTTCTTCGAg                                                                <  1:943317/150‑1 (MQ=255)                                                                          cAGGTTCTGGCGCACGTCTTCGGTGGCCTGGTCGATGCTGTGTTGCAGTTCTTCGTGGGCATTCTGCAGGGTTTC‑GCCATGCGGTTGATGCCGTGGGCCAGTTCGTCCAGCTCATGGCTGCCCATGGTCGGCAGGCGTTCTTCGAGGTgg                                                            >  2:108022/1‑150 (MQ=255)                                                                           aGGTTCTGGCGCACGTCTTCGGTGGCCTGGTCGATGCTGTGTTGCAGTTCTTCGTGGGCATTCTGCAGGGTTTC‑GCCATGCGGTTGATGCCGTGGGCCAGTTCGTCCAGCTCATGGCTGCCCATGGTCGGCAGGCGTTCTTCGAGGTGGc                                                           >  2:1118962/1‑150 (MQ=255)                                                                                       aCGTCTTCGGTGGCCTGGTCGATGCTGTGTTGCAGTTCTTCGTGGGCATTCTGCAGGGTTTC‑GCCATGCGGTTGATGCCGTGGGCCAGTTCGTCCAGCTCATGGCTGCCCATGGTCGGCAGGCGTTCTTCGAGGTGGCCGTCCTTGAGCt                                               >  1:866255/1‑150 (MQ=255)                                                                                         gTCTTCGGTGGCCTGGTCGATGCTGTGTTGCAGTTCTTCGCGGGCATTTTGCAGGGTTTC‑GCAATGCGGTGGATGCCGTGGGCCAGTTCGTCCAGCTCATGGCTGCCCATGGTCGGCAGGCGTTCTTCGAGGTGGCCGTCCTTGAGCTGa                                             >  1:1076476/1‑150 (MQ=255)                                                                                                           gATGCTGTGTTGCAGTTCTTCGTGGGCATTCTGCAGGGTTTC‑GCCATGCGGTTGATGCCGTGGGCCAGTTCGTCCAGCTCATGGCTGCCCATGGTCGGCAGGCGTTCTTCGAGGTGGCCGTCCTTGAGCTGATTGACCGCGTGCTTGATg                           >  2:729085/1‑150 (MQ=255)                                                                                                            aTGCTGTGTTGCAGTTCTTCGTGGGCATTCTGCAGGGTTTC‑GCCATGCGGTTGATGCCGTGGGCCAGTTCGTCCAGCTCATGGCTGCCCATGGTCGGCAGGCGTTCTTCGAGGTGGCCGTCCTTGAGCTGATTGACCGCGTGCTTGATGc                          >  1:717090/1‑150 (MQ=255)                                                                                                               cTGTGTTGCAGTTCTTCGTGGGCATTCTGCAGGGTTTC‑GCCATGCGGTTGATGCCGTGGGCCAGTTCGTCCAGCTCATGGCTGCCCATGGTCGGCAGGCGTTCTTCGAGGTGGCCGTCCTTGAGCTGATTGACCGCGTGCTTGATGCGtt                       <  1:942324/150‑1 (MQ=255)                                                                                                                   gtTGCAGTTCTTCGTGGGCATTCTGCAGGGTTTC‑GCCATGCGGTTGATGCCGTGGGCCAGTTCGTCCAGCTCATGGCTGCCCATGGTCGGCAGGCGTTCTTCGAGGTGGCCGTCCTTGAGCTGATTGACCGCGTGCTTGATGCGTTCGAt                   <  2:1191708/150‑1 (MQ=255)                                                                                                                      gCAGTTCTTCGTGGGCATTCTGCAGGGTTTC‑GCCATGCGGTTGATGCCGTGGGCCAGTTCGTCCAGCTCATGGCTGCCCATGGTCGGCAGGCGTTCTTCGAGGTGGCCGTCCTTGAGCTGATTGACCGCGTGCTTGa                             >  1:707551/1‑137 (MQ=255)                                                                                                                      gCAGTTCTTCGTGGGCATTCTGCAGGGTTTC‑GCCATGCGGTTGATGCCGTGGGCCAGTTCGTCCAGCTCATGGCTGCCCATGGTCGGCAGGCGTTCTTCGAGGTGGCCGTCCTTGAGCTGATTGACCGCGTGCTTGa                             <  2:707551/137‑1 (MQ=255)                                                                                                                      gCAGTTCTTCGTGGGCATTCTGCAGGGTTTC‑GCCATGCGGTTGATGCCGTGGGCCAGTTCGTCCAGCTCATGGCTGCCCATGGTCGGCAGGCGTTCTTCGAGGTGGCCGTCCTTGAGCTGATTGACCGCGTGCTTGATGCGTTCGATggg                <  1:40582/150‑1 (MQ=255)                                                                                                                      gCAGTTCTTCGTGGGCATTCTGCAGGGTTTC‑GCCATGCGGTTGATGCCGTGGGCCAGTTCGTCCAGCTCATGGCTGCCCATGGTCGGCAGGCGTTCTTCGAGGTGGCCGTCCTTGAGCTGATTGACCGCGTGCTTGATGCGTTCGATggg                <  2:445427/150‑1 (MQ=255)                                                                                                                       cAGTTCTTCGTGGGCATTCTGCAGGGTTTC‑GCCATGCGGTTGATGCCGTGGGCCAGTTCGTCCAGCTCATGGCTGCCCATGGTCGGCAGGCGTTCTTCGAGGTGGCCGTCCTTGAGCTGATTGACCGCGTGCTTGATGCGTTCGATgggg               <  1:108022/150‑1 (MQ=255)                                                                                                                              tcGTGGGCATTCTGCAGGGTTTC‑GCCATGCGGTTGATGCCGTGGGCCAGTTCGTCCAGCTCATGGCTGCCCATGGTCGGCAGGCGTTCTTCGAGGTGGCCGTCCTTGAGCTGATTGACCGCGTGCTTGATGCGTTCGATGGGGTCGTTGa        <  1:732757/150‑1 (MQ=255)                                                                                                                              tcGTGGGCATTCTGCAGGGTTTC‑GCCATGCGGTTGATGCCGTGGGCCAGTTCGTCCAGCTCATGGCTGCCCATGGTCGGCAGGCGTTCTTCGAGGTGGCCGTCCTTGAGCTGATTGACCGCGTGCTTGATGCGTTCGATGGGGTCGTTGa        <  2:732453/150‑1 (MQ=255)                                                                                                                                gTGGGCATTCTGCAGGGTTTC‑GCCATGCGGTTGATGCCGTGGGCCAGTTCGTCCAGCTCATGGCTGCCCATg                                                                                    >  2:1389652/1‑72 (MQ=255)                                                                                                                                gTGGGCATTCTGCAGGGTTTC‑GCCATGCGGTTGATGCCGTGGGCCAGTTCGTCCAGCTCATGGCTGCCCATg                                                                                    <  1:1389652/72‑1 (MQ=255)                                                                                                                                    gCATTCTGCAGGGTTTC‑GCCATGCGGTTGATGCCGTGGGCCAGTTCGTCCAGCTCATGGCTGCCCATGGTCGGCAGGCGTTCTTCGAGGTGGCCGTCCTTGAGCTGATTGACCGCGTGCTTGATGCGTTCGATGGGGTCGTTGATGGTGc  <  2:1076476/150‑1 (MQ=255)                                                                                                                                     cATTCTGCAGGGTTTC‑GCCATGCGGTTGATGCCGTGGGCCAGt                                                                                                            >  2:871806/1‑43 (MQ=255)                                                                                                                                     cATTCTGCAGGGTTTC‑GCCATGCGGTTGATGCCGTGGGCCAGt                                                                                                            <  1:871806/43‑1 (MQ=255)                                                                                                                                      aTTCTGCAGGGTTTC‑GCCATGCGGTTGATGCCGTGGGCCAGTTCGTCCCACTCAGGGCTGCCCATGGTCGGCAGGCGTTCTTCGAGGTGGCCGTCCTTGAGCTGATTGACcgcg                                    >  1:255003/1‑114 (MQ=255)                                                                                                                                      aTTCTGCAGGGTTTC‑GCCATGCGGTTGATGCCGTGGGCCAGTTCGTCCAGCTCATGGCTGCCCATGGTCGGCAGGCGTTCTTCGAGGTGGCCGTCCTTGAGCTGATTGACcgcg                                    <  2:255003/114‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                      ACTTGATGCGGCTGGCCTCAAGTGCCTCTTTGCGCGCCATGTCCAGCTCGATGTTCTGGATTTCGATGGTTTCCAGGTTCTGGCGCACGTCTTCGGTGGCCTGGTCGATGCTGTGTTGCAGTTCTTCGTGGGCATTCTGCAGGGTTTCGGCCATGCGGTTGATGCCGTGGGCCAGTTCGTCCAGCTCATGGCTGCCCATGGTCGGCAGGCGTTCTTCGAGGTGGCCGTCCTTGAGCTGATTGACCGCGTGCTTGATGCGTTCGATGGGGTCGTTGATGGTGC  >  NC_002947/1843977‑1844258

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑