Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_002947 1,157,858 +T intergenic (+89/‑16) yihS → / → gtsA aldose‑ketose isomerase/mannose/glucose ABC transporter substrate‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,157,8581.T97.7% 137.5 / ‑4.7 44intergenic (+89/‑16)yihS/gtsAaldose‑ketose isomerase/mannose/glucose ABC transporter substrate‑binding protein
Reads supporting (aligned to +/- strand):  ref base . (1/0);  new base T (19/24);  total (20/24)
Fisher's exact test for biased strand distribution p-value = 4.55e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AGGTTTGTCCCTGGCACCCAGCCTGGCCAGCAACCTGGCCGGCAATGTAACCAAACGATGACATTCACGCATCGCTTCGTTACCTGACGAGGCGGGCACGCTGTTTAGACTTCATGCTACGCAAGCGATCGACTTGCACGGCATAACAAC‑AA‑GAAAGGTGCTCCGATGAATTCCACGCTTCGTCTCGCTGCCGCAATTTCCTTTGCCTCGTTAATCCCCTTGGGTGCCCAGGCTGCTGACGCCAAAGGCAGTGTCGAAGTGGTGCACTGGTGGACCTCCGGTGGTGAAAAAGC  >  NC_002947/1157709‑1158001
                                                                                                                                                      |                                                                                                                                                
aGGTTTGTCCCTGGCACCCAGCCTGGCCAGCAACCTGGCCGGCAATGTAACCAAACGATGACATTCACGCATCGCTTCGTTACCTGACGAGGCGGGCACGCTGTTTAGACTTCATGCTACGCAAGCGATCGACTTGCACGGCATaacaac                                                                                                                                                   >  2:1301416/1‑150 (MQ=255)
   tttGTCCCTGGCACCCAGCCTGGCCAGCAACCTGGCCGGCAATGTAACCAAACGATGACATTCACGCATCGCTTCGTTACCTGACGAGGCGGGCACGCTGTTTAGACTTCATGCTACGCAAGCGATCGACTTGCACGGCATaacaactaa                                                                                                                                                <  2:526944/150‑4 (MQ=255)
   tttGTCCCTGGCACCCAGCCTGGCCAGCAACCTGGCCGGCAATGTAACCAAACGATGACATTCACGCATCGCTTCGTTACCTGACGAGGCGGGCACGCTGTTTAGACTTCATGCTACGCAAGCGATCGACTTGCACGGCATaacaactaa                                                                                                                                                <  2:447449/150‑4 (MQ=255)
    ttGTCCCTGGCACCCAGCCTGGCCAGCAACCTGGCCGGCAATGTAACCAAACGATGACATTCACGCATCGCTTCGTTACCTGACGAGGCGGGCACGCTGTTTAGACTTCATGCTACGCAAGCGATCGACTTGCACGGCATaacaactaag                                                                                                                                               <  2:1150780/150‑5 (MQ=255)
         ccTGGCACCCAGCCTGGCCAGCAACCTGGCCGGCAATGTAACCAACCGATGACATTCACGCATCGCTTCGTTACCTGACGAGGCGGGCACGCTGTTTAGACTTCATGCTACGCAAGCGATCGACTTGCACGGCATAACAACTAA‑GAAAgg                                                                                                                                         <  1:693572/150‑1 (MQ=255)
          cTGGCACCCAGCCTGGCCAGCAACCTGGCCGGCAATGTAACCAAACGATGACATTCACGCATCGCTTCGTTACCTGACGAGGCGGGCACGCTGTTTAGACTTCATGCTACGCAAGCGATCGACTTGCACGGCATAACAACTAA‑GAAAGGt                                                                                                                                        <  1:175540/150‑1 (MQ=255)
              cACCCAGCCTGGCCAGCAACCTTGCCGGCAATGTAACCAAACGATGACATTCACGCATCGCTTCGTTACCTGACGAGGCGGGCACGCTGTTTAGACTTCATGCTACGCAAGCGATCGACTTGCACGGCATAACAACTAA‑GAAAGGTGCTc                                                                                                                                    <  2:526210/150‑1 (MQ=255)
              cACCCAGCCTGGCCAGCAACCTGGCCGGCAATGTAACCAAACGATGACATTCACGCATCGCTTCGTTACCTGACGAGGCGGGCACGCTGTTTAGACTTCATGCTACGCAAGCGATCGACTTGCACGGCATAACAACTAA‑GAAAGGTGCTc                                                                                                                                    >  1:253161/1‑150 (MQ=255)
                 ccAGCCTGGCCAGCAACCTGGCCGGCAATGTAACCAAACGATGACATTCACGCATCGCTTCGTTACCTGACGAGGCGGGCACGCTGTTTAGACTTCATGCTACGCAAGCGATCGACTTGCACGGCATAACAACTAA‑GAAAGGTGCTCCGa                                                                                                                                 >  2:470698/1‑150 (MQ=255)
                  cAGCCTGGCCAGCAACCTGGCCGGCAATGTAACCAAACGATGACATTCACGCATCGCTTCGTTACCTGACGAGGCGGGCACGCTGTTTAGACTTCATGCTACGCAAGCGATCGACTTGCACGGCATAACAACTAA‑GAAAGGTGCTCCGAt                                                                                                                                <  1:201472/150‑1 (MQ=255)
                  cAGCCTGGCCAGCAACCTGGCCGGCAATGTAACCAAACGATGACATTCACGCATCGCTTCGTTACCTGACGAGGCGGGCACGCTGTTTAGACTTCATGCTACGCAAGCGATCGACTTGCACGGCATAACAACTAA‑GAAAGGTGCTCCGAt                                                                                                                                <  1:730364/150‑1 (MQ=255)
                        ggCCAGCAACCTGGCCGGCAATGTAACCAAACGATGACATTCACGCATCGCTTCGTTACCTGACGAGGCGGGCACGCTGTTTAGACTTCATGCTACGCAAGCGATCGACTTGCACGGCATAACAACTAA‑GAAAGGTGCTCCGATGAATTc                                                                                                                          >  1:1392904/1‑150 (MQ=255)
                                 ccTGGCCGGCAATGTAACCAAACGATGACATTCACGCATCGCTTCGTTACCTGACGAGGCGGGCACGCTGTTTAGACTTCATGCTACGCAAGCGATCGACTTGCACGGCATaacaactaa                                                                                                                                                <  1:216517/120‑4 (MQ=255)
                                 ccTGGCCGGCAATGTAACCAAACGATGACATTCACGCATCGCTTCGTTACCTGACGAGGCGGGCACGCTGTTTAGACTTCATGCTACGCAAGCGATCGACTTGCACGGCATaacaactaa                                                                                                                                                >  2:216517/1‑117 (MQ=255)
                                        ggCAATGTAACCAAACGATGACATTCACGCATCGCTTCGTTACCTGACGAGGCGGGCACGCTGTTTAGACTTCATGCTACGCAAGCGATCGACTTGCACGGCATAACAACTAA‑GAAAGGTGCTCCGa                                                                                                                                 <  1:983918/127‑1 (MQ=255)
                                        ggCAATGTAACCAAACGATGACATTCACGCATCGCTTCGTTACCTGACGAGGCGGGCACGCTGTTTAGACTTCATGCTACGCAAGCGATCGACTTGCACGGCATAACAACTAA‑GAAAGGTGCTCCGa                                                                                                                                 >  2:983918/1‑127 (MQ=255)
                                           aaTGTAACCAAACGATGACATTCACGCATCGCTTCGTTACCTGACGAGGCGGGCACGCTGTTTAGACTTCATGCTACGCAAGCGATCGACTTGCACGGCATAACAACTAA‑GAAAGGTGCTCCGATGAATTCCACGCTTCGTCTCGCTGcc                                                                                                       <  2:588570/150‑1 (MQ=255)
                                               tAACCAAACGATGACATTCACGCATCGCTTCGTTACCTGACGAGGCGGGCACGCTGTTTAGACTTCATGCTACGCAAGCGATCGAATTGCACGGAATAACGAT‑AAGGAAATGTGCTCCGATGAATTCCACGCTTCGTCTCGCTGCCGCaa                                                                                                   >  1:313646/1‑150 (MQ=255)
                                                aaCCAAACGATGACATTCACGCATCGCTTCGTTACCTGACGAGGCGGGCACGCTGTTTAGACTTCATGCTACGCAAGCGATCGACTTGCACGGCATAACAACTAA‑GAAAGGTg                                                                                                                                       >  2:280637/1‑113 (MQ=255)
                                                aaCCAAACGATGACATTCACGCATCGCTTCGTTACCTGACGAGGCGGGCACGCTGTTTAGACTTCATGCTACGCAAGCGATCGACTTGCACGGCATAACAACTAA‑GAAAGGTg                                                                                                                                       <  1:280637/113‑1 (MQ=255)
                                                    aaaCGATGACATTCACGCATCGCTTCGTTACCTGACGAGGCGGGCACGCTGTTTAGACTTCATGCTACGCAAGCGATCGACTTGCACGGCATAACAACTAA‑GAAAGGTGCTCCGATGAATTCCACGCTTCGTCTCGCTGCCGCAATTTcc                                                                                              >  1:1206797/1‑150 (MQ=255)
                                                              aTTCACGCATCGCTTCGTTACCTGACGAGGCGGGCACGCTGTTTAGACTTCATGCTACGCAAGCGATCGACTTGCACGGCATAACAACTAA‑GAAAGGTGCTCCGATGAATTCCACGCTTCGTCTCGCTGCCGCAATTTCCTTTGCCTCGt                                                                                    <  1:417716/150‑1 (MQ=255)
                                                                  aCGCATCGCTTCGTTACCTGACGAGGCGGGCACGCTGTTTAGACTTCATGCTACGCAAGCGATCGACTTGCACGGCATAACAACTAA‑GAAAGGTGCTCCGATGAATTCCACGCTTc                                                                                                                  <  2:68065/116‑1 (MQ=255)
                                                                  aCGCATCGCTTCGTTACCTGACGAGGCGGGCACGCTGTTTAGACTTCATGCTACGCAAGCGATCGACTTGCACGGCATAACAACTAA‑GAAAGGTGCTCCGATGAATTCCACGCTTc                                                                                                                  >  1:68065/1‑116 (MQ=255)
                                                                     cATCGCTTCGTTACCTGACGAGGCGGGCACGCTGTTTAGACTTCATGCTACGCAAGCGATCGACTTGCACGGCATAACAACTAA‑GAAAGGTGCTCCGATGAATTCCACGCTTCGTCTCGCTGCCGCAATTTCCTTTGCCTCGTTAATccc                                                                             >  1:1227031/1‑150 (MQ=255)
                                                                        cGCTTCGTTACCTGACGAGGCGGGCACGCTGTTTAGACTTCATGCTACGCAAGCGATCGACTTGCACGGCATAACAACTAA‑GAAAGGTGCTCCGATGAATTCCACGCTTCGTCTCGCTGCCGCAATTTCCTTTGCCTCGTTGCTCACCtt                                                                          <  2:1392904/150‑1 (MQ=255)
                                                                         gCTTCGTTACCTGACGAGGCGGGCACGCTGTTTAGACTTCATGCTACGCAAGCGATCGACTTGCACGGCATAACAACTAA‑GAAAGGTGCTCCGATGAATTCCACGCTTCGTCTCGCTGCCGCAATTTCCTTTGCCTCGTTAATCCCCTTg                                                                         <  1:1257708/150‑1 (MQ=255)
                                                                             cGTTACCTGACGAGGCGGGCACGCTGTTTAGACTTCATGCTACGCAAGCGATCGACTTGCACGGCATAACAACTAA‑GAAAGGTGCTACGATGAATTCCACGCTTCGTCTCGCTGCCGCAATTTCCTTTGCCTCGTTAATCCCCTTGGGTg                                                                     <  1:552187/150‑1 (MQ=255)
                                                                              gTTACCTGACGAGGCGGGCACGCTGTTTAGACTTCATGCTACGCAAGCGATCGACTTGCACGGCATAACAACTAA‑GAAAGGTGCTCCGATGAATTCCACGCTTCGTCTCGCTGCCGCAATTTCCTTTGCCTCGTTAATCCCCTTGGGTGc                                                                    <  1:1317178/150‑1 (MQ=255)
                                                                                   cTGACGAGGCGGGCACGCTGTTTAGACTTCATGCTACGCAAGCGATCGACTTGCACGGCATAACAACTAA‑GAAAGGTGCTCCGATGAATTCCACGCTTCGTCTCGCTGCCGCAATTTCCTTTGCCTCGTTAATCCCCTTGGGTGCCCAgg                                                               >  2:499750/1‑150 (MQ=255)
                                                                                      aCGAGGCGGGCACGCTGTTTAGACTTCATGCTACGCAAGCGATCGACTTGCACGGCATAACAACTAA‑GAAAGGTGCTCCGATGAATTCCACGCTTCGTCTCGCTGCCGCAATTTCCTTTGCCTCGTTAATCCCCTTGGGTGCCCAGgctg                                                            >  2:287273/1‑150 (MQ=255)
                                                                                                   gCTGTTTAGACTTCATGCTACGCAAGCGATCGACTTGCACGGCATAACAACTAA‑GAAAGGTGCTCCGATGAATTCCACGCTTCGTCTCGCTGCCGCAATTTCCTTTGCCTCGTTAATCCCCTTGGGTGCCCAGGCTGCTGACGCCAAAgg                                               >  1:463803/1‑150 (MQ=255)
                                                                                                     tGTTTAGACTTCATGCTACGCAAGCGATCGACTTGCACGGCATAACAACTAA‑GAAAGGTGCTCCGATGAATTCCACGCTTCGTCTCGCTGCCGCAATTTCCTTTGCCTCGTTAATCCCCTTGGGTGc                                                                    >  1:778646/1‑127 (MQ=255)
                                                                                                     tGTTTAGACTTCATGCTACGCAAGCGATCGACTTGCACGGCATAACAACTAA‑GAAAGGTGCTCCGATGAATTCCACGCTTCGTCTCGCTGCCGCAATTTCCTTTGCCTCGTTAATCCCCTTGGGTGc                                                                    <  2:778646/127‑1 (MQ=255)
                                                                                                        ttAGACTTCATGCTACGCACGCGATCGACTTGCAAGGCATAACCACTAA‑GAAAGGTGCTACGATGAATTCCACGCTTCGTCTCGCTGCCGCAATTTCCTTTGCCTCGTTAATCCCCTTGGGTGCCCAGGCTGCTGACGCCAAAGGCAgtg                                          <  1:649669/150‑1 (MQ=255)
                                                                                                             cTTCATGCTACGCAAGCGATCGACTTGCACGGCATAACAACTAA‑GAAAGGTGCTCCGATGAATTCCACGCTTCGTCTCGCTGCCGCAATTTCCTTTGCCTCGTTAATCCCCTTGGGTGCCCAGGCTGCTGACGCCAAAGGCAGTGTCGaa                                     <  2:313646/150‑1 (MQ=255)
                                                                                                              ttCATGCTACGCAAGCGAACGACTTGCACGGAATTACAACTAA‑GAAAGGTGCTCCGATGAATTCCACGCTTCGGCTCGCTGCCGCAATTTCCTTTGCCTCGTTAATCCCCTTGGTTTCCCAGGCTGCTGACGCCAACGGCCGTGTCTAAg                                    >  2:292412/1‑150 (MQ=255)
                                                                                                                cATGCTACGCAAGCGATCGACTTGCACGGCATAACAACTAA‑GAAAGGTGCTCCGATGAATTCCACGCTTCGTCTCGCTGCCGCAATTTCCTTTGCCTCGTTAATCCCCTTGGGTGCCCAGGCTGCTGACGCCAAAGGCAGTGTCGAAgtg                                  <  1:470698/150‑1 (MQ=255)
                                                                                                                  tGCTACGCAAGCGATCGACTTGCACGGCATAACAACTAA‑GAAAGGTGCTCCGATGAATTCCACGCTTCGTCTCGCTGCCGCAATTTCCTTTGCCTCGTTAATCCCCTTGGGTGCCCAGGCTGCTGACGCCAAAGGCAGTGTCGAAgtggt                                <  2:1206797/150‑1 (MQ=255)
                                                                                                                  tGCTACGCAAGCGATCGACTTGCACGGCATAACAACTAA‑GAAAGGTGCTCAGATGAATTCCACGCTTCGTCTCGCTGCCGCAATTTCCTTTGCCTCGTTGATCCTCGTGGGTGCCCAGGCTGCTGACGCCAAAGGCAGTGTCGAAgtggt                                <  1:1301416/150‑1 (MQ=255)
                                                                                                                   gCTACGCAAGCGATCGACTTGCACGGCATAACAACTAA‑GAAAGGTGCTCCGATGAATTCCACGCTTCGTCTCGCTGCCGCAATTTCCTTTGCCTCGTTAATCCCCTTGGGTGCCCAGGCTGCTGACGc                                                     >  2:75930/1‑128 (MQ=255)
                                                                                                                   gCTACGCAAGCGATCGACTTGCACGGCATAACAACTAA‑GAAAGGTGCTCCGATGAATTCCACGCTTCGTCTCGCTGCCGCAATTTCCTTTGCCTCGTTAATCCCCTTGGGTGCCCAGGCTGCTGACGc                                                     <  1:75930/128‑1 (MQ=255)
                                                                                                                             cGATCGACTTGCACGGCATAACAACTAA‑GAAAGGTGCTCCGATGAATTCCACGCTTCGTCTCGCTGCCGCAATTTCCTTTGCCTCGTTAATCCCCTTGGGTGCCCAGGCTGCTGACGCCAAAGGCAGTGTCGAAGTGGTGCACTGGTGGa                     <  2:253161/150‑1 (MQ=255)
                                                                                                                              gATCGACTTGCACGGCATAACAACTAA‑GAAAGGTGCTCCGATGAATTCCACGCTTCGTCTCGCTGCCGCAATTTCCTTTGCCTCGTTAATCCCCTTGGGTGCCCAGGCTGCTGACGCCAAAGGCAGTGTCGAAGTGGTGCACtggt                        >  2:429081/1‑146 (MQ=255)
                                                                                                                              gATCGACTTGCACGGCATAACAACTAA‑GAAAGGTGCTCCGATGAATTCCACGCTTCGTCTCGCTGCCGCAATTTCCTTTGCCTCGTTAATCCCCTTGGGTGCCCAGGCTGCTGACGCCAAAGGCAGTGTCGAAGTGGTGCACtggt                        <  1:429081/146‑1 (MQ=255)
                                                                                                                              gATCGACTTGCACGGCATAACAACTAA‑GAAAGGTGCTCCGATGAATTCCACGCTTCGTCTCGCTGCCGCAATTTCCTTTGCCTCGTTAATCCCCTTGGGTGCCCAGGCTGCTGACGCCAAAGGCAGTGTCGAAGTGGTGCACTGGTGGAc                    >  2:914793/1‑150 (MQ=255)
                                                                                                                                  gACTTGCACGGCATAACAACTAA‑GAAAGGTGCTCCGATGAATTCCACGCTTCGTCTCGCTGCCGCAATTTCCTTTGCCTCGTTAATCCCCTTGGGTGCCCAGGCTGCTGACGCCAAAGGCAGTGTCGAAGTGGTGCACTGGTGGACCTcc                >  2:491610/1‑150 (MQ=255)
                                                                                                                                         aCGGCATAACAACTAA‑GAAAGGTGCTCCGATGAATTCCACGCTTCGTCTCGCTGCCGCAATTTCCTTTGCCTCGTTAATCCCCTTGGGTGCCCAGGCTGCTGACGCCAAAGGCAGTGTCGAAGTGGTGCACt                           >  1:81531/1‑132 (MQ=255)
                                                                                                                                         aCGGCATAACAACTAA‑GAAAGGTGCTCCGATGAATTCCACGCTTCGTCTCGCTGCCGCAATTTCCTTTGCCTCGTTAATCCCCTTGGGTGCCCAGGCTGCTGACGCCAAAGGCAGTGTCGAAGTGGTGCACt                           <  2:81531/132‑1 (MQ=255)
                                                                                                                                               tAACAACTAA‑GAAAGGTGCTCCGATGAATTCCACGCTTCGTCTCGCTGCCGCAATTTCCTTTGCCTCGTTAATCCCCTTGGGTGCCCAGGCTGCTGACGCCAAAGGCAGTGTCGAAGTGGTGCACTGGTGGACCTCCGGTGGTGAAAAAg   >  1:1123699/1‑150 (MQ=255)
                                                                                                                                                aacaactAA‑GAAAGGTGCTCCGATGAATTCCACGCTTCGTCTCGCTGCCGCAATTTCCTTTGCCTCGTTAATCCCCTTGAGTGCCCAGGCTGCTGACGCCAAAGGCAGTGTCGAAGTGGTGCACTGGTGGACCTCCGGGGGTGAAAAAgc  >  2:112959/1‑150 (MQ=255)
                                                                                                                                                      |                                                                                                                                                
AGGTTTGTCCCTGGCACCCAGCCTGGCCAGCAACCTGGCCGGCAATGTAACCAAACGATGACATTCACGCATCGCTTCGTTACCTGACGAGGCGGGCACGCTGTTTAGACTTCATGCTACGCAAGCGATCGACTTGCACGGCATAACAAC‑AA‑GAAAGGTGCTCCGATGAATTCCACGCTTCGTCTCGCTGCCGCAATTTCCTTTGCCTCGTTAATCCCCTTGGGTGCCCAGGCTGCTGACGCCAAAGGCAGTGTCGAAGTGGTGCACTGGTGGACCTCCGGTGGTGAAAAAGC  >  NC_002947/1157709‑1158001

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑