Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_002947 1,851,555 T→A L200Q (CTG→CAG)  relA → ATP:GTP 3'‑pyrophosphotransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,851,5550TA95.5% 136.1 / ‑2.6 44L200Q (CTG→CAG) relAATP:GTP 3'‑pyrophosphotransferase
Reads supporting (aligned to +/- strand):  ref base T (1/1);  new base A (15/27);  total (16/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CATGGTCGACGACGTGCGCGTGGCGCTGATCAAGCTGGCCGAACGCACGTGCGCAATCCGGGCGGTCAAGTCCGCCGATGACGAGAAACGCCTGCGTGTCGCGCGTGAAGTGTTCGACATCTACGCGCCGCTCGCGCACCGCCTGGGTATCGATCACATCAAGTGGGAGCTGGAAGACCTGTCCTTCCGCTACCTGGAGCCCGACCAGTACAAGCAGATCGCCAAGCTGTTGCATGAGCGGCGGCTGGACCGCGAGCGCTTCATCAGCGACGTGATGAAC  >  NC_002947/1851412‑1851691
                                                                                                                                               |                                                                                                                                        
cATGGTCGACGACGTGCGCGTGGCGCTGATCAAGCTGGCCGAACGCACGTGCGCAATCCGGGCGGTCAAGTCCGCCGATGACGAGAAACGCATGCGTTGCACGCCGAAAGTGTTCGACATCTACGCGCCGCTCGCGCACCGCCAGGGTAt                                                                                                                                    >  2:36019/1‑150 (MQ=255)
  tGGTCGACGACGTGCGCGTGGCGCTGATCAAGCTGGCCGAACGCACGTGCGCAATCCGGGCGGTCAAGTCCGCCGATGACGAGAAACGCCTGCGTGTCGCGCGTGAAGTGTTCGACATCTACGCGCCGCTCGCGCACCGCCAGGGTATCg                                                                                                                                  <  2:56491/150‑1 (MQ=255)
     tCGACGACGTGCGCGTGGCGCTGATCAAGCTGGCCGAACGCACGTGCGCAATCCGGGCGGTCAAGTCCGCCGATGACGAGAAACGCCTGCGTGTCGCGCGTGAAGTGTTCGACATCTACGCGCCGCTCGCGCACCGCCAGGGTATCGATc                                                                                                                               <  1:138492/150‑1 (MQ=255)
        acgacgTGCGCGTGGCGCTGATCAAGCTGGCCGAACGCACGTGCGCAATCCGGGCGGTCAAGTCCGCCGATGACGAGAAACGCCTGCGTGTCGCGCGTGAAGTGTTCGACATCTACGCCCGCCACGCGCACCGCCAGGGTATCGATcaca                                                                                                                            <  1:36019/150‑1 (MQ=255)
         cgacgTGCGCGTGGCGCTGATCAAGCTGGCCGAACGCACGTGCGCAATCCGGGCGGTCAAGTCCGCCGATGACGAGAAACGCCTGCGTGTCGCGCGTGAAGTGTTCGACATCTACGCGCCGCTCGCGCACCGCCAGGGTATCGATCACAt                                                                                                                           <  2:171441/150‑1 (MQ=255)
              tGCGCGTGGCGCTGATCAAGCTGGCCGAACGCACGTGCGCAATCCGGGCGGTCAAGTCCGCCGATGACGAGAAACGCCTGCGTGTCGCGCGTGAAGTGTTCGACATCTACGCGCCGCTCGCGCACCGCCAGGGTATCGATCACATCAAGt                                                                                                                      <  2:455962/150‑1 (MQ=255)
               gcgcGTGGCGCTGATCAAGCTGGCCGAACGCACGTGCGCAATCCGGGCGGTCAAGTCCGCCGATGACGAGAAACGCCTGCGTGTCGCGCGTGAAGTGTTCGACATCTACGCGCCGCTCGCGCACCGCCAGGGTATCGATCACATCAAGTg                                                                                                                     <  2:1274301/150‑1 (MQ=255)
                cgcgTGGCGCTGATCAAGCCGGCCGAACGCACGTGCGCAATCCGGGCGGTCAAGTCCGCCGATGACGAGAAACGCCTGCGTGTCGCGCGTGAAGTGTTCGACATCTACGCGCCGCTCGCGCACCGCCAGGGTATCGATCACATCAAGTgg                                                                                                                    <  1:962164/150‑1 (MQ=255)
                            aTCAAGCTGGCCGAACGCACGTGCGCAATCCGGGCGGTCAAGTCCGCCGATGACGAGAAACGCCTGCGTGTCGCGCGTGAAGTGTTCGACATCTACGCGCCGCTCGCGCACCGCCAGGGTATCGATCACATCAAGTGGGAGCTGGAAGAc                                                                                                        >  2:560974/1‑150 (MQ=255)
                              cAAGCTGGCCGAACGCACGTGCGCAATCCGGGCGGTCAAGTCCGCCGATGACGAGAAACGCCTGCGTGTCGCGCGTGAAGTGTTCGACATCTACGCGCCGCTCGCGCACCGCCAGGGTATCGATCACATCAAGTGGGAGCTGGAAGACCt                                                                                                      <  2:442704/150‑1 (MQ=255)
                                aGCTGGCCGAACGCACGTGCGCAATCCGGGCGGTCAAGTCCGCCGATGACGAGAAACGCCTGCGTGTCGCGCGTGAAGTGTTCGACATCTACGCGCCGCTCGCGCACCGCCAGGGTATCGATCACATCAAGTGGGAGCTGGAAGACCTGt                                                                                                    <  2:1258109/150‑1 (MQ=255)
                                 gCTGGCCGAACGCACGTGCGCAATCCGGGCGGTCAAGTCCGCCGATGACGAGAAACGCCTGCGTGTCGCGCGTGAAGTGTTCGACATCTACGCGCCGCTCGCGCACCGCCAgg                                                                                                                                        <  1:12679/113‑1 (MQ=255)
                                 gCTGGCCGAACGCACGTGCGCAATCCGGGCGGTCAAGTCCGCCGATGACGAGAAACGCCTGCGTGTCGCGCGTGAAGTGTTCGACATCTACGCGCCGCTCGCGCACCGCCAgg                                                                                                                                        >  2:12679/1‑113 (MQ=255)
                                     gCCGAACGCACGTGCGCAATCCGGGCGGTCAAGTCCGCCGATGACGAGAAACGCCTGCCTGTCGCGCGTGAAGTGTTCGACATCTACGCGCCGCTCGCGCACCGCCAGGGTATCGATCACATCAAGTGGGAGCTGGAAGACCTGTCCTTc                                                                                               >  2:352376/1‑150 (MQ=255)
                                            gCACGTGCGCAATCCGGGCGGTCAAGTCCGCCGATGACGAGAAACGCCTGCGTGTCGCGCGTTAAGTGTTCGACATCTACGCGCCGCTCGCGCACCGCCAGGGTATCGATCACATCAAGTGGGAGCTGGAAGACCTGTCCTTCCGCTAcc                                                                                        <  2:160729/150‑1 (MQ=255)
                                                       aTCCGGGCGGTCAAGTCCGCCGATGACGAGAAACGCCTGCGTGTCGCGCGTGAAGTGTTCGACATCTACGCGCCGCTCGCGCACCGCCAGGGTATCGATCACATCAAGTGGGAGCTGGAAGACCTGTCCTTCCGCTACCTGGAGCCCGAc                                                                             <  2:818007/150‑1 (MQ=255)
                                                        tCCGGGCGGTCAAGTCCGCCGATGACGAGAAACGCCTGCGTGTCGCGCGTGAAGTGTTCGACATCTACGCGCCGCTCGCGCACCGCCTGGGTATCGATCACATCAAGTGGGAGCTGGAAGACCTGTCCTTCCGCTACCTGGAGCCCGAcc                                                                            >  1:389171/1‑150 (MQ=255)
                                                          cGGGCGGTCAGGCCCGCCGATGGCGAGAAACGCCTGCGTGTCGCGCGTGAAGTGTTCGACATCTACGCGCCGCTCGCGCACCGCCAGGGTATCGATCACATCAAGTGGGAGCTGGAAGACCTGTCCTTGCGCTACCTGGAGCCCGACCAg                                                                          >  2:719195/1‑150 (MQ=255)
                                                             gCGGTCAAGTCCGCCGATGACGAGAAACGCCTGCGTGTCGCGCGTGAGGTGTTCGACATCTACGCGCCGCCCGCACACCGCCAGGGGATCGATCACATCAAGTGGGAGCTGGACAACATGTACATCCGCGACCTGAGTCCCGACCAgatc                                                                       >  2:301080/1‑147 (MQ=255)
                                                               ggTCAAGTCCGCCGATGACGAGAAACGCCTGCGTGTCGCGCGTGAAGTGTTCGACATCTACGCGCCGCTCGCGCACCGCCAGGGTATCGATCACATCAAGTGGGAGCTGGAAGACCTGTCCTTCCGCTACCTGGAGCCCGACCAGTACaa                                                                     >  2:1024401/1‑150 (MQ=255)
                                                                gTCAAGTCCGCCGATGACGAGAAACGCCTGCGTGTCGCGCGTGAAGTGTTCGACATCTACGCGCCGCTCGCGCACCGCCAGGGTATCGATCACATCAAGTGGGAGCTGGAAGACCTGTCCTTCCGCTACCTGGAGCCCGACCAGTACAAg                                                                    <  1:560974/150‑1 (MQ=255)
                                                                      tCCGCCGATGACGAGAAACGCCTGCGTGTCGCGCGTGAAGTGTTCGACATCTACGCGCCGCTCGCGCACCGCCAGGGTATCGATCACATCAAGTGGGAGCTg                                                                                                              >  2:518346/1‑102 (MQ=255)
                                                                      tCCGCCGATGACGAGAAACGCCTGCGTGTCGCGCGTGAAGTGTTCGACATCTACGCGCCGCTCGCGCACCGCCAGGGTATCGATCACATCAAGTGGGAGCTg                                                                                                              <  1:518346/102‑1 (MQ=255)
                                                                      tCCGCCGATGACGAGAAACGCCTGCGTGTCGCGCGTGAAGTGTTCGACATCTACGCGCCGCTCGCGCACCGCCAGGGTATCGATCACATCAAGTGGGAGCTGGaa                                                                                                           >  2:543512/1‑105 (MQ=255)
                                                                      tCCGCCGATGACGAGAAACGCCTGCGTGTCGCGCGTGAAGTGTTCGACATCTACGCGCCGCTCGCGCACCGCCAGGGTATCGATCACATCAAGTGGGAGCTGGaa                                                                                                           <  1:543512/105‑1 (MQ=255)
                                                                                  gagaAACGCCTGCGTGTCGCGCGTGAAGTGTTCGACATCTACGCGCCGCTCGCGCACCGCCAGGGTATCGATCACATCAAGTGGGAGCTGGAAGACCTGTCCTTCCGCTACCTGGAGCCCGACCAGTACAAGCAGATCGCCAAGCTGTTg                                                  >  2:137188/1‑150 (MQ=255)
                                                                                   agaAACGCCTGCGTGTCGCGCGTGAAGTGTTCGACATCTACGCGCCGCTCGCGCACCGCCAGGGTATCGATCACATCAAGTGGGAGCTGGAAGACCTGTCCTTCCGCTACCTGGAGCCCGACCAGTACAAGCAGATCGCCAAGCTGTTGc                                                 <  2:446801/150‑1 (MQ=255)
                                                                                    gaAACGCCTTCGTGTCGCGCGTGAAGTGTTCGACATCTACGCGCCGCTCGCGCACCGCCAGGGTATCGATCACATCAAGTGGGAGCTGGAAGACCTGTCCTTCCGCTACCTGGAGCCCGACCAGTACAAGCAGATCGCCAAGCTGTTGCa                                                >  2:202246/1‑150 (MQ=255)
                                                                                    gaAACGCCTGCGTGTCGCGCGTGAAGTGTTCGACATCTACGCGCCGCTCGCGCACCGCCAGGGTATCGATCACATCAAGTGGGAGCTGGAAGACCTGTCCTTCCGCTACCTGGAGCCCGACCAGTACAAGCAGATCGCCAAGCTGTTGCa                                                <  2:673719/150‑1 (MQ=255)
                                                                                         gCCTGCGTGTCGCGCGTGAAGTGTTCGACATCTACGCGCCGCTCGCGCACCGCCAGGGTATCGATCACATCAAGTGGGAGCTGGAAGACCTGTc                                                                                                   >  2:596868/1‑94 (MQ=255)
                                                                                         gCCTGCGTGTCGCGCGTGAAGTGTTCGACATCTACGCGCCGCTCGCGCACCGCCAGGGTATCGATCACATCAAGTGGGAGCTGGAAGACCTGTc                                                                                                   <  1:596868/94‑1 (MQ=255)
                                                                                                     cgcgTGAAGTGTTCGACATCTACGCGCCGCTCGCGCACCGCCAGGGTATCGATCACATCAAGTGGGAGCTGGAAGACCTGTCCTTCCGCTACCTGGAGCCCGACCAGTACAAGCAGATCGCCAAGCTGTTGCATGAGCGGCGGCTGGAcc                               >  1:586651/1‑150 (MQ=255)
                                                                                                             gtgtTCGACATCTACGCGCCGCTCGCGCACCGCCAGGGTATCGATCACATCAAGTGGGAGCTGGAAGACCTGTCCATCCCCTACCTGGAGCCCGACCAGTACAAGCAGATCGCCAAGCTGTTGCATGAGCGGCGGCTGGACCGCGAgcgc                       <  1:523252/150‑1 (MQ=255)
                                                                                                              tgtTCGACATCTACGCGCCGCTCGCGCACCGCCAGGGTATCGATCACATCAAGTGGGAGCTGGAAGACCTGTCCTTCCGCTACCTGGAGCCCGACCAGTACAAGCAGATCGCCAAGCTGTTGCATGAGCGGCGGCTGGACCGCGAGCGCt                      <  1:1024401/150‑1 (MQ=255)
                                                                                                                ttCGACATCTACGCGCCGCTCGCGCACCGCCAGGGTATCGATCACATCAAGTGGGAGCTGGAAGACCTGTCCTTCCGCTACCTGGAGCCCGACCAGTACAAGCAGATCGCCAAGCTGTTGCATGAGCGGCGGCTGGACCGCGAGCGCTtc                    >  2:67388/1‑150 (MQ=255)
                                                                                                                ttCGACATCTACGCGCCGCTCGCGCACCGCCAGGGTATCGATCACATCAAGTGGGAGCTGGAAGACCTGTCCTTCCGCTACCTGGAGCCCGACCAGTACAAGCAGATCGCCAAGCTGTTGCATGAGCGGCGGCTGGACCGCGAGCGCTtc                    <  2:737594/150‑1 (MQ=255)
                                                                                                                ttCGACATCTACGCGCCGCTCGCGCACCGCCAGGGTATCGATCACATCAAGTGGGAGCTGGAAGACCTGTCCTTCCGCTACCTGGAGCCCGACCAGTACAAGCAGATCGCCAAGCTGTTGCACGAGCGGCGGCTGGACCGCGAGCGCTtc                    <  1:352376/150‑1 (MQ=255)
                                                                                                                   gACATCTACGCGCCGCTCGCGCACCGCCAGGGTATCGATCACATCAAGTGGGAGCTGGAAGACCTGTCCTTCCGCTACCTGGAGCCCGACCAGTACAAGCAGATCGCCAAGCTGTTGCATGAGCGGCGGCTGGACCGCGAGCGCTtcatc                 <  1:67388/150‑1 (MQ=255)
                                                                                                                     cATCTACGCGCCGCTCGCGCACCGCCAGGGTATCGATCACATCAAGTGGGAGCTGGAAGACCTGTCCTTCCGCTACCTGGAGCCCGACCAGTACAAGCAGATCGCCAAGCTGTTGCATGAGCGGCGGCTGGACCGCGAGCGCTTCATCAg               <  1:301080/150‑1 (MQ=255)
                                                                                                                           cgcgCCGCTCGCGCACCGCCTGGGTATCGATCACATCAAGTGGGAGCTGGAAGACCTGTCCTTCCGCTACCTGGAGCCCGACCAGTACAAGCAGATCGCCAAGCTGTTGCATGAGCGGCGGCTGGACCGCGAGCGCTTCATCAGCGACGt         <  2:389171/150‑1 (MQ=255)
                                                                                                                             cgccgcTCGCGCACCGCCAGGGTATCGATCACATCAAGTGGGAGCTGGAAGACCTGTCCTTCCGCTACCTGGAGCCCGACCAGTACAAGCAGATCGCCAAGCTGTTGCATGAGCGGCGGCTGGACCGCGAGCGCTTCATCAGCGACGtga       <  2:123285/150‑1 (MQ=255)
                                                                                                                                  cTCGCGCACCGCCAGGGTATCGATCACATCAAGTGGGAGCTGGAAGACCTGTCCTTCCGCTACCTGGAGCCCGACCAGTACAAGCAGATCGACAAGCTGTTGCATGAGCGGCGGCTGGACCGCGAGCGCTTCATCAGCGACGTGATGAAc  <  2:295751/150‑1 (MQ=255)
                                                                                                                                           cGCCAGGGTATCGATCACATCAAGTGGGAGCTGGAAGACCTGTCCTTCCGCTACCTGGAGCCCGACCAGTACAAGCAGATCGCCAAGCTGTTGCATGAGCGGCGGCTGGACCGCGAGCGCTtc                    <  1:419607/123‑1 (MQ=255)
                                                                                                                                           cGCCAGGGTATCGATCACATCAAGTGGGAGCTGGAAGACCTGTCCTTCCGCTACCTGGAGCCCGACCAGTACAAGCAGATCGCCAAGCTGTTGCATGAGCGGCGGCTGGACCGCGAGCGCTtc                    >  2:419607/1‑123 (MQ=255)
                                                                                                                                               |                                                                                                                                        
CATGGTCGACGACGTGCGCGTGGCGCTGATCAAGCTGGCCGAACGCACGTGCGCAATCCGGGCGGTCAAGTCCGCCGATGACGAGAAACGCCTGCGTGTCGCGCGTGAAGTGTTCGACATCTACGCGCCGCTCGCGCACCGCCTGGGTATCGATCACATCAAGTGGGAGCTGGAAGACCTGTCCTTCCGCTACCTGGAGCCCGACCAGTACAAGCAGATCGCCAAGCTGTTGCATGAGCGGCGGCTGGACCGCGAGCGCTTCATCAGCGACGTGATGAAC  >  NC_002947/1851412‑1851691

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑