Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,282,8180GC57.5% ‑4.2 / 50.5 40intergenic (‑167/+62)PP_2010/PP_2011cytochrome b561/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (8/9);  new base C (11/12);  total (19/21)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.70e-01
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

AATGGCGCTCTTGCTGGCGCTCAGGAGTCATTTTAATGGCTACCGGAAACAGATCTTTGCGCCAGGTCTGTGAAAAATGTGTTGCCAGCCACCGGCCTCTTCGCTGGCACGCCCGCTCCCACAGTCCAACTGCACAGTCTTGGAAGCCTGTGCAGTTGGACTGTGGGAGCGGGCGTGCCCGCGAAGGGGCCGG‑AGCAGGCGACATCAGTTACCGGGCAGATAGGGCCAGTCATCACCCACCAGAAACACCCGTTCTGCCTCCTGCCAGGCACCATCGGCATCA  >  NC_002947/2282676‑2282958
                                                                                                                                              |                                                                                                                                             
aaTGGCGCTCTTGCTGGCGCTCAGGAGTCATTTTAATGGCTACCGGAAACAGATCTTTGCGCCAGGTCTGTGAAAAATGTGTTGCCAGCCACCGGCCCCTTCGCGGGCACGCCCGCTCCCACAGTCCAACTGCACAGTCTTGGAAGCCtg                                                                                                                                        <  2:457529/150‑1 (MQ=255)
   ggCGCTCTTGCTGGCGCTCAGGAGTCATTTTAATGGCTACCGGAAACAGATCTTTGCGCCAGGTCTGTGAAAAATGTGTTGCCAGCCACCGGCCTCTTCGCTGGCACGCCCGCTCCCACAGTCCAACTGCACAGGCTTCCAAGACTGTGc                                                                                                                                     <  1:259841/150‑1 (MQ=37)
      gcTCTTGCTGGCGCTCAGGAGTCATTTTAATGGCTACCGGAAACAGTTCTTTGCGCCAGGTCTGTGAAAAATGTGTTGCCAGCCACCGGCCTCTTCGCTGGCACGCCCGCTCCCACAGTCCAACTGCACAGGCTTCCAAGACTGTGCAGt                                                                                                                                  <  1:62317/150‑1 (MQ=37)
       ctctTGCTGGCGCTCAGGAGTCATTTTAATGGCTACCGGAAACAGATCTTTGCGCCAGGTCTGTGAAAAATGTGTTGCCAGCCACCGGCCTCTTCGCTGGCACGCCCGCTCCCACAGTCCAACTGCACAGTCTTGGAAGCCTGTGCAGtt                                                                                                                                 <  2:49310/150‑1 (MQ=255)
       ctctTGCTGGCGCTCAGGAGTCATTTTAATGGCTACCGGAAACAGATCTTTGCGCCAGGTCTGTGAAAAATGTGTTGCCAGCCACCGGCCTCTTCGCTGGCACGCCCGCTCCCACAGTCCAACTGCACAGTCTTGGAAGCCTGTGCAGtt                                                                                                                                 <  1:791261/150‑1 (MQ=255)
             cTGGCGCTCAGGAGTCATTTTAATGGCTACCGGAAACAGATCTTTGCGCCAGGTCTGTGAAAAATGTGTTGCCAGCCACCGGCCTCTTCGCTGGCACGCCNGNTCCCACNGTCCAACTGCACNGTCTTGGAAGCCTGTGCAGTTGGANtg                                                                                                                           <  1:692719/150‑1 (MQ=255)
                  gcTCAGGAGTCATTTTAATGGCTACCGGAAACAGATCTTTGCGCCAGGTCTGTGAAAAATGTGTTGCCAGCCACCGGCCTCTTCGCTGGCACGCCCGCTCCCACAGTCCAACTGCACAGTCTTGGAAGCCtgtg                                                                                                                                      <  1:929864/134‑1 (MQ=255)
                  gcTCAGGAGTCATTTTAATGGCTACCGGAAACAGATCTTTGCGCCAGGTCTGTGAAAAATGTGTTGCCAGCCACCGGCCTCTTCGCTGGCACGCCCGCTCCCACAGTCCAACTGCACAGGCTTCCAAGACTGTGCAGTTGGACTGTGGGa                                                                                                                      <  2:393756/150‑1 (MQ=255)
                  gcTCAGGAGTAATTTTAATGGCTACCGGAAACAGATCTTTGCGCCAGGTCTGTGAAAAATGTGTTGCCAGCCACCGGCCTCTTCGCTGGCACGCCCGCTCCCACAGTCCAACTGCACAGTCTTGGAAGCCtgtg                                                                                                                                      >  2:929864/1‑134 (MQ=255)
                        gAGTCATTTTAATGGCTACCGGAAACAGATCTTTGCGCCAGGTCTGTGAAAAATGTGTTGCCAGCCACCGGCCTCTTCGCTGGCACGCCCGCTCCCACAGTCCAACTGCACAGTCTTGGAAGCCTGTGCAGTTGGACTGTGGGAGCGGGc                                                                                                                <  2:677324/150‑1 (MQ=255)
                              ttttAATGGCTACCGGAAACAGATCTTTGCGCCAGGTCTGTGAAAAATGTGTTGCCAGCCACCGGCCTCTTCGCTGGCACGCCCGCTCCCACAGTCCAACTGCACAGTCTTGGAAGCCt                                                                                                                                         <  1:596447/119‑1 (MQ=255)
                              ttttAATGGCTACCGGAAACAGATCTTTGCGCCAGGTCTGTGAAAAATGTGTTGCCAGCCACCGGCCTCTTCGCTGGCACGCCCGCTCCCACAGTCCAACTGCACAGTCTTGGAAGCCt                                                                                                                                         >  2:596447/1‑119 (MQ=255)
                              ttttAATGGCTACCGGAAACAGATCTTTGCGCCAGGTCTGTGAAAAATGTGTTGCCAGCCACCGGCCTCTTCGCTGGCACGCCCGCTCCCACAGTCCAACTGCACAGGCTTCCAAGACTGTGCAGTTggc                                                                                                                              <  2:234266/130‑2 (MQ=25)
                              ttttAATGGCTACCGGAAACAGATCTTTGCGCCAGGTCTGTGAAAAATGTGTTGCCAGCCACCGGCCTCTTCGCTGGCACGCCCGCTCCCACAGTCCAACTGCACAGGCTTCCAAGACTGTGCAGTTGGa                                                                                                                              >  1:234266/1‑130 (MQ=25)
                                      gCTACCGGAAACAGATCTTTGCGCCAGGTCTGTGAAAAATGTGTTGCCAGCCACCGGCCTCTTCGCTGGCACGCCCGCTCCCACAGTCCAACTGCACAGTCTTGGAAGCCTGTGCAGTTGGACTGTGGGAGCGGGCGTGCCCGCGAAggg                                                                                                  >  2:503722/1‑150 (MQ=255)
                                                 cAGATCTTTGCGCCAGGTCTGTGAAAAATGTGTTGCCAGCCACCGGCCTCTTCGCTGGCACGCCCGCTCCCACAGTCCAACTGCACAGTCTTGGAAGCCTGTGCAGTTGGACTGTGGGAGCGGGCGTGCCCGCGAAGGGGCCGG‑AGCAgg                                                                                      <  1:503722/150‑1 (MQ=255)
                                                                    tgtgAAAAATGTGTTGCCAGCCACCGGCCTCTTCGCTGGCACGCCCGCTCCCACAGTCCAACTGCACAGGCTTCCAAGACTGTGCAGTTGGACTGTgg                                                                                                                        >  1:800917/1‑98 (MQ=17)
                                                                    tgtgAAAAATGTGTTGCCAGCCACCGGCCTCTTCGCTGGCACGCCCGCTCCCACAGTCCAACTGCACAGGCTTCCAAGACTGTGCAGTTGGACTGTgg                                                                                                                        <  2:800917/98‑1 (MQ=17)
                                                                       gAAAAATGTGTTGCCAGCCACCGGCCTCTTCGCTGGCACGCCCGCTCCCACAGTCCAACTGCACAGGCTTCCAAGACTGTGCAGtt                                                                                                                                 >  2:322399/1‑86 (MQ=14)
                                                                       gAAAAATGTGTTGCCAGCCACCGGCCTCTTCGCTGGCACGCCCGCTCCCACAGTCCAACTGCACAGGCTTCCAAGACTGTGCAGtt                                                                                                                                 <  1:322399/86‑1 (MQ=14)
                                                                                          tccGGCCCCTTCGCGGGCACGCCCGCTCCCACAGTCCAACTGCACAGGCTTCCAAGACTGTGCAGTTGGACTGTGGGAGCGGGCGTGCCAGCGAAGAGGCCGG‑AGCAGGCGACATCAGTTACCGGGCAGATAGGGCCAGTCATcacccac                                             >  1:63110/2‑150 (MQ=14)
                                                                                                              gCCCGCTCCCACAGTCCAACTGCACAGTCTTGGAAGCCTGTGCAGTTGGACTGTGGGAGCGGGCGTGCCCGCGAAGGGGCCGG‑AGCAGGCGACATCAGTTACCGGGCAGATAGGGCCAGTCATCACCCACCAGAAACACCCGTTCTGcct                         >  2:702697/1‑150 (MQ=255)
                                                                                                                      ccACAGTCCAACTGCACAGTCTTGGAAGCCTGTGCAGTTGGACTGTGGGAGCGGGCGTGCCCGCGAAGGGGCCGG‑AGCAGGCGACATCAGTTACCGGGCAGATAGGGCCAGTCATCACCCACCAGAAACACCCGTTCTGCCTCCTGCCAg                 >  2:883270/1‑150 (MQ=255)
                                                                                                                      ccACAGTCCAACTGCACAGTCTTGGAAGCCTGTGCAGTTGGACTGTGGGAGCGGGCGTGCCCGCGAAGGGGCCGG‑AGCAGGCGACATCAGTTACCGGGCAGATAGGGCCAGTCATCACCCACCAGAAACACCCGTTCTGCCTCCTGCCAg                 <  1:702697/150‑1 (MQ=255)
                                                                                                                       cacaGTCCAACTGCACAGGCTTCCAAGACTGTGCAGTTGGACTGTGGGAGCGGGCGTGCCCGCGAAGGGGCCGG‑AGCAGGCGACATCAGTTACCGGGCAGATAGGGCCAGTCATCACCCACCAGAAACACCCGTTCTGCCTCCTGCCAgg                >  1:301029/1‑150 (MQ=25)
                                                                                                                          aGTCCAACTGCACAGGCTTCCAAGACTGTGCAGTTGGACTGTGGGAGCGGGCGTGCCCGCGAAGGGGCCGG‑AGCAGGCGACATCAGTTACCGGGCAGATAGGGCCa                                                         >  1:412063/1‑106 (MQ=18)
                                                                                                                          aGTCCAACTGCACAGGCTTCCAAGACTGTGCAGTTGGACTGTGGGAGCGGGCGTGCCCGCGAAGGGGCCGG‑AGCAGGCGACATCAGTTACCGGGCAGATAGGGCCa                                                         <  2:412063/106‑1 (MQ=18)
                                                                                                                            tCCATCTGCACAGGCTTCCAAGACTGTGCAGTTGGACTGTGGGAGCGGGCGTGCCCGCGAAGGGGCCGG‑AGCAGGCGACATCAGTTACCGGGCAGATAGGGCCAGTCATCACCCACCAGAAACACCCGTTCTGCCTCCTGCCAGGCa              <  1:859113/147‑1 (MQ=37)
                                                                                                                            tCCATCTGCACAGGCTTCCAAGACTGTGCAGTTGGACTGTGGGAGCGGGCGTGCCCGCGAAGGGGCCGG‑AGCAGGCGACATCAGTTACCGGGCAGATAGGGCCAGTCATCACCCACCAGAAACACCCGTTCTGCCTCCTGCCAGGCa              >  2:859113/1‑147 (MQ=37)
                                                                                                                                aCTGCACAGGCTTCCAAGACTGTGCAGTTGGACTGTGGGAGCGGGCGTGCCAGCGAAGAGGCCGGTGGCTGGCAACATCAGTTACCGGGCAGATAGGGCCAGTCATCACCCACCAGAAACAcc                                   <  2:326877/123‑1 (MQ=11)
                                                                                                                                aCTGCACAGGCTTCCAAGACTGTGCAGTTGGACTGTGGGAGCGGGCGTGCCAGCGAAGAGGCCGGTGGCTGGCAACATCAGTTACCGGGCAGATAGGGCCAGTCATCACCCACCAGAAACAcc                                   >  1:326877/1‑123 (MQ=11)
                                                                                                                                 cTGCACAGTCTTGGAAGCCTGTGCAGTTGGACTGTGGGAGCGGGCGTGCCCGCGAAGGGGCCGG‑AGCAGGCGACATCAGTTACCGGGCAGATAGGGCCAGTCATCACCCACCAGAAACACCCGTTCTGCCTCCTGACAGGCACCATCGGc      >  1:418404/1‑150 (MQ=255)
                                                                                                                                 cTGCACAGGCTTCCAAGACTGTGCAGTTGGACTGTGGGAGCGGGCGTGCCCGCGAAGGGGCCGG‑AGCAGGCGACATCAGTTACCGGGCAGATAGGGCCAGTCATCACCCACCAGAAACACCCGTTCTGCCTCCTGCCAGGCACCATCg        >  2:555913/1‑148 (MQ=255)
                                                                                                                                 cTGCACAGGCTTCCAAGACTGTGCAGTTGGACTGTGGGAGCGGGCGTGCCCGCGAAGGGGCCGG‑AGCAGGCGACATCAGTTACCGGGCAGATAGGGCCAGTCATCACCCACCAGAAACACCCGTTCTGCCTCCTGCCAGGCACCATCg        <  1:555913/148‑1 (MQ=255)
                                                                                                                                  tGCACAGTCTTGGAAGCCTGTGCAGTTGGAATGTGGGAGCGGGCGTGCCCGCGAAGGGGCCGG‑AGCAGGCGACATCAGTTACCGGGCAGATAGGGCCAGTCATCACCCACCAGAAACACCCGTTCTGCCTCCTGCCAGGCACCATCGGCa     >  2:231027/1‑150 (MQ=255)
                                                                                                                                  tGCACAGGCTTCCAAGACTGTGCAGTTGGACTGTGGGAGCGGGCGTGCCCGCGAAGGGGCCGG‑AGCAGGCGACATCAGTTACCGGGCAGATAGGGCCAGTCATCACCCACCAGAAACACCCGTTCTGCCTCCTGCCAGGCACCATCGGCa     >  2:44699/1‑150 (MQ=38)
                                                                                                                                  tGCACAGGCTTCCAAGACTGTGCAGTTGGACTGTGGGAGCGGGCGTGCCAGCGAAGAGGCCGG‑AGCAGGCGACATCAGTTACCGGGCAGATAGGGCCAGTCATCACCCACCAGAAACACCCGTTCTGCCTCCTGCCAGGCACCATCGGCa     <  2:63110/150‑1 (MQ=25)
                                                                                                                                  tGCACAGGCTTCCAAGACTGTGCAGTTGGACTGTGGGAGCGGGCGTGCCAGCGAAGAGGCCGG‑AGCAGGCGACATCAGTTACCGGGCAGATAGGGCCAGTCATCACCCACCAGAAACACCCGTTCTGCCTCCTGCCAGGCACCATCGGCa     >  2:330696/1‑150 (MQ=25)
                                                                                                                                     accgTCTTGGAAGCCTGTGCAGTTGGACTGTGGGAGCGGGCGTGCCCGCGAAGGGGCCGG‑AGCAGGCGACATCAGTTACCGGGCAGATAGGGCCAGTCATCACCCACCAGAAACACCCGTTCTGCCTCCTGCCAGGCACCATCGGCATCa  >  2:108552/4‑150 (MQ=255)
                                                                                                                                     acaGGCTTCCAAGACTGTGCAGTTGGACTGTGGGAGCGGGCGTGCCAGCGAAGAGGCCGG‑AGCAGGCGACATCAGTTACCGGGCAGATAGGGCCAGTCATCACCCACCAGAAACACCCGTTCTGCCTCCTGCCAGGCACCATCGGCATCa  <  1:330696/150‑1 (MQ=37)
                                                                                                                                             ggAAGCCTGTGCAGTTGGACTGTGGGAGCGGGCGTGCCCGCGAAGGGGCCGG‑AGCAGGCGACATCAGTTACCGGGCAGATAGGGCCAGTCATCACCCACCAGAAACACCCGTTCTGCCTCCTGCCAGGCACCATCGGCATCa  <  2:356642/142‑1 (MQ=255)
                                                                                                                                             ggAAGCCTGTGCAGTTGGACTGTGGGAGCGGGCGTGCCCGCGAAGGGGCCGG‑AGCAGGCGACATCAGTTACCGGGCAGATAGGGCCAGTCATCACCCACCAGAAACACCCGTTCTGCCTCCTGCCAGGCACCATCGGCATCa  >  1:356642/1‑142 (MQ=255)
                                                                                                                                             ggAAGACTGTGCAGTTGGACTGTGGGAGCGGGCGTGCCAGCGAAGAGGCCGG‑TGCAGGCGACATCAGTTACCGGGCAGATAGGGCCAGTCATCACCCACCAGAAACACCCGTTCt                             >  2:855505/1‑115 (MQ=25)
                                                                                                                                             ggAAGACTGTGCAGTTGGACTGTGGGAGCGGGCGTGCCAGCGAAGAGGCCGG‑TGCAGGCGACATCAGTTACCGGGCAGATAGGGCCAGTCATCACCCACCAGAAACACCCGTTCt                             <  1:855505/115‑1 (MQ=25)
                                                                                                                                              |                                                                                                                                             
AATGGCGCTCTTGCTGGCGCTCAGGAGTCATTTTAATGGCTACCGGAAACAGATCTTTGCGCCAGGTCTGTGAAAAATGTGTTGCCAGCCACCGGCCTCTTCGCTGGCACGCCCGCTCCCACAGTCCAACTGCACAGTCTTGGAAGCCTGTGCAGTTGGACTGTGGGAGCGGGCGTGCCCGCGAAGGGGCCGG‑AGCAGGCGACATCAGTTACCGGGCAGATAGGGCCAGTCATCACCCACCAGAAACACCCGTTCTGCCTCCTGCCAGGCACCATCGGCATCA  >  NC_002947/2282676‑2282958

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑