Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_002947	1,851,537	C→T	A194V (GCG→GTG)	relA →	ATP:GTP 3'‑pyrophosphotransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,851,537	0	C	T	100.0%	174.5 / NA	52	A194V (GCG→GTG)	relA	ATP:GTP 3'‑pyrophosphotransferase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (24/28);  total (24/28)

CGCAAGATGCTGGTGGCCATGGTCGACGACGTGCGCGTGGCGCTGATCAAGCTGGCCGAACGCACGTGCGCAATCCGGGCGGTCAAGTCCGCCGATGACGAGAAACGCCTGCGTGTCGCGCGTGAAGTGTTCGACATCTACGCGCCGCTCGCGCACCGCCTGGGTATCGATCACATCAAGTGGGAGCTGGAAGACCTGTCCTTCCGCTACCTGGAGCCCGACCAGTACAAGCAGATCGCCAAGCTGTTGCATGAGCGGCGGCTGGACCGCGAGCGCTTCA  >  NC_002947/1851395‑1851674                                                                                                                                               |                                                                                                                                          cgcAAGATGCTGGTGGCCATGGTCGACGACGTGCGCGTGGCGCTGATCAAGCTGGCCGAACGCACGTGCGCAATCCGGGCGGTCAAGTCCGCCGATGACGAGAAACGCCTGCGTGTCGCGCGTGAAGTGTTCGACATCTACGTGCCGCTc                                                                                                                                    >  2:1030009/1‑150 (MQ=255)         gCTGGTGGCCATGGTCGACGACGTGCGCGTGGCGCTGATCAAGCTGGCCGAACGCACGTGCGCAATCCGGGCGGTCAAGTCCGCCGATGACGAGAAACGCCTGCGTGTCGCGCGTGAAGTGTTCGACATCTACGTGCCGCTCGCGCACCg                                                                                                                            >  2:13710/1‑150 (MQ=255)         gCTGGTGGCCATGGTCGACGACGTGCGCGTGGCGCTGATCAAGCTGGCCGAACGCACGTGCGCAATCCGGGCGGTCAAGTCCGCCGATGACGAGAAACGCCTGCGTGTCGCGCGTGAAGTGTTCGACATCTACGTGCCGCTCGCGCACCg                                                                                                                            >  1:566355/1‑150 (MQ=255)             gtggCCATGGTCGACGACGTGCGCGTGGCGCTGATCAAGCTGGCCGAACGCACGTGCGCAATCCGGGCGGTCAAGTCCGCCGATGACGAGAAACGCCTGCGTGTCGCGCGTGAAGTGTTCGACATCTACGTGCCGCTCGCGCACCGCCTg                                                                                                                        <  2:1240501/150‑1 (MQ=255)              tggCCATGGTCGCCGACGTGAGCGTGGCGCTGATCAAGCTGGCCGAACGCACGTGCGCAATCCGGTCGGTCAAGTCCGCCGATGACGAGAAACGCCTGCGTGTCGCGCGTGAAGTGTTCGACATCTACGTGCCGCTCGCGCACCGCCTgg                                                                                                                       <  2:798178/150‑1 (MQ=255)              tggCCATGGTCGACGACGTGCGCGTGGCGCTGATCAAGCTGGCCGAACGCACGTGCGCAATCCGGGCGGTCAAGTCCGCCGATGACGAGAAACGCCTGCGTGTCGCGCGTGAAGTGTTCGACATCTACGTGCCGCTCGCGCACCGCCTgg                                                                                                                       <  1:1030009/150‑1 (MQ=255)              tggCCATGGTCGACGACGTGCGCGTGGCGCTGATCAAGCTGGCCGAACGCACGTGCGCAATCCGGGCGGTCAAGTCCGCCGATGACGAGAAACGCCTGCGTGTCGCGCGTGAAGTGTTCGACATCTACGTGCCGCTCGCGCACCGCCTgg                                                                                                                       <  2:249424/150‑1 (MQ=255)              tggCCATGGTCGACGACGTGCGCGTGGCGCTGATCAAGCTGGCCGAACGCACGTGCGCAATCCGGGCGGTCAAGTCCGCCGATGACGAGAAACGCCTGCGTGTCGCGCGTGAAGTGTTCGACATCTACGTGCCGCTCGCGCACCGCCTgg                                                                                                                       <  1:13710/150‑1 (MQ=255)                 ccATGGTCGACGACGTGCGCGTGGCGCTGATCAAGCTGGCCGAACGCACGTGCGCAATCCGGGCGGTCAAGTCCGCCGATGACGAGAAACGCCTGCGTGTCGCGCGTGAAGTGTTCGACATCTACGTGCCGCTCGCGCACCGCCTgg                                                                                                                       >  2:1080795/1‑147 (MQ=255)                 ccATGGTCGACGACGTGCGCGTGGCGCTGATCAAGCTGGCCGAACGCACGTGCGCAATCCGGGCGGTCAAGTCCGCCGATGACGAGAAACGCCTGCGTGTCGCGCGTGAAGTGTTCGACATCTACGTGCCGCTCGCGCACCGCCTgg                                                                                                                       <  1:1080795/147‑1 (MQ=255)                  cATGGTCGACGACGTGCGCGTGGCGCTGATCAAGCTGGCCGAACGCACGTGCGCAATCCGGGCGGTCAAGTCCGCCGATGACGAGAAACGCCTGCGTGTCGCGCGTGAAGTGTTCGACATCTACGTGCCGCTCGCGCACCGCCTGGGTAt                                                                                                                   >  1:985969/1‑150 (MQ=255)                        cgacgaCGTGCGCGTGGCGCTGATCAAGCTGGCCGAACGCACGTGCGCAATCCGGGCGGTCAAGTCCGCCGATGACGAGAAACGCCTGCGTGTCGCGCGTGAAGTGTTCGACATCTACGTGCCGCTCGCGCACCGCCTGGGTATCGATca                                                                                                             >  1:657885/1‑150 (MQ=255)                            gacgTGCGCGTGGCGCTGATCAAGCTGGCCGAACGCACGTGCGCAATCCGGGCGGTCAAGTCCGCCGATGACGAGAAACGCCTGCGTGTCGCGCGTGAAGTGTTCGACATCTACGTGCCGCTCGCGCACCGCCTGGGTATCGATCACATc                                                                                                         >  1:149928/1‑150 (MQ=255)                              cgTGCGCGTGGCGCTGATCAAGCTGGCCGAACGCACGTGCGCAATCCGGGCGGTCAAGTCCGCCGATGACGAGAAACGCCTGCGTGTCGCGCCTGATCGTCTCGACATCTACGTGCCGCTCGCGCACCGCCTGGGTATCGATCACATCaa                                                                                                       <  2:820732/150‑1 (MQ=255)                                tGCGCGTGGCGCTGATCAAGCTGGCCGAACGCACGTGCGCAATCCGGGCGGTCAAGTCCGCCGATGACGAGAAACGCCTGCGTGTCGCGCGTGAAGTGTTCGACATCTACGTGCCGCTCGCGCACCGCCTGGGTATCGATCACATCAAGt                                                                                                     <  2:867153/150‑1 (MQ=255)                                 gcgcGTGGCGCTGATCAAGCTGGCCGAACGCACGTGCGCAATCCGGGCGGTCAAGTCCGCCGATGACGAGAAACGCCTGCGTGTCGCGCGTGAAGTGTTCGACATCTACGTGCCGCTCGCGCACCGCCTGGGTATCGATCACATCAAGTg                                                                                                    >  1:1306890/1‑150 (MQ=255)                                     gTGGCGCTGATCAAGCTGGCCGAACGCACGTGCGCAATCCGGGCGGTCAAGTCCGCCGATGACGAGAAACGCCTGCGTGTCGCGCGTGAAGTGTTCGACATCTACGTGCCGCTCGCGCACCGCCTGGGTATCGATCACATCAAGTGGGAg                                                                                                <  2:566355/150‑1 (MQ=255)                                           cgaaTCAAGCTGGCCGAACGCACGTGCGCAATCCGGGCGGTCAAGTCCGCCGATGACGAGAAACGCCTGCGTGTCGCGCGTGAAGTGTTCGACATCTACGTGCCGCTCGCGCACCGCCTGGGTATCGATCACATCAAGTGGGAGCTGGaa                                                                                          <  1:65959/147‑1 (MQ=255)                                                 aaGCTGGCCGAACGCACGTGCGCAATCCGGGCGGTCAAGTCCGCCGATGACGAGAAACGCCTGCGTGTCGCGCGTGAAGTGTTCGACATCTACGTGCCGCTCGCGCACCGCCTGGGTATCGATCACATCAAg                                                                                                      <  1:464279/132‑1 (MQ=255)                                                 aaGCTGGCCGAACGCACGTGCGCAATCCGGGCGGTCAAGTCCGCCGATGACGAGAAACGCCTGCGTGTCGCGCGTGAAGTGTTCGACATCTACGTGCCGCTCGCGCACCGCCTGGGTATCGATCACATCAAg                                                                                                      >  2:464279/1‑132 (MQ=255)                                                    cTGGCCGAACGCACGTGCGCAATCCGGGCGGTCAAGTCCGCCGATGACGAGAAACGCCTGCGTGTCGCGCGTGAAGTGTTCGACATCTACGTGCCGCTCGCGCACCGCCTGGGTATCGATCACATCAAGTGGGAGCTGGAAGACCTGTcc                                                                                 >  2:880568/1‑150 (MQ=255)                                                      ggCCGAACGCACGTGCGCAATCCGGGCGGTCAAGTCCGCCGATGACGAGAAACGCCTGCGTGTCGCGCGTGAAGTGTTCGACATCTACGTGCCGCTCGCGCACCGCCTGGGTATCGATCACATCAAGTGGGAGCTGGAAGACCTGTCCtt                                                                               <  2:149928/150‑1 (MQ=255)                                                        ccGAACGCACGTGCGCAATCCGGGCGGTCAAGTCCGCCGATGACGAGAAACGCCTGCGTGTCGCGCGTGAAGTGTTCGACATCTACGTGCCGCTCGCGCACCGCCTGGGTATCGATCACATCAAGTGGGAGCTGGAAGACCTGTCCTTcc                                                                             >  1:457409/1‑150 (MQ=255)                                                           aaCGCACGTGCGCAATCCGGGCGGTCAAGTCCGCCGATGACGAGAAACGCCTGCGTGTCGCGCGTGAAGTGTTCGACATCTACGTGCCGCTCGCGCACAGCCTGGGTATCGATCACATCAAGTGGGAGCTGGAAGACCTGTCCTTCCGCt                                                                          <  1:384235/150‑1 (MQ=255)                                                               cACGTGCGCAATCCGGGCGGTCAAGTCCGCCGATGACGAGAAACGCCTGCGTGTCGCGCGTGAAGTGTTCGACATCTACGTGCCGCTCGCGCACCGCCTGGGTATCGATCACATCAAGTGGGAGCTGGAAGACCTGTCCTTCCGCTACCt                                                                      <  2:938787/150‑1 (MQ=255)                                                                aCGTGTGCAATCCGGGCGGTCAAGTCCGCCGATGACCAGAAACGCCTGCGTGTCGCGCGTGAAGTGTTCGACATCTACGTGCCGCTCGCGCACCCCCTGGGTATCGATCACATCAAGTGGGAGCTGGAAGACATGTCCTTCCGCTACCTg                                                                     >  2:211999/1‑150 (MQ=255)                                                                   tGCGCAATCCGGGCGGTCAAGTCCGCCGATGACGAGAAACGCCTGCGTGTCGCGCGTGAAGTGTTCGACATCTACGTGCCGCTCGCGCACCGCCTGGGTATCGATCACATCAAGTGGGAGCTGGAAGACCTGTCCTTCCGCTACCTGGAg                                                                  <  1:1616208/150‑1 (MQ=255)                                                                        aaTCCGGGCGGTCAAGTCCGCCGATGACGAGAAACGCCTGCGTGTCGCGCGTGAAGTGTTCGACATCTACGTGCCGCTCGCGCACCGCCTGGGTATCGATCACATCAAGt                                                                                                     >  1:1618064/1‑110 (MQ=255)                                                                        aaTCCGGGCGGTCAAGTCCGCCGATGACGAGAAACGCCTGCGTGTCGCGCGTGAAGTGTTCGACATCTACGTGCCGCTCGCGCACCGCCTGGGTATCGATCACATCAAGt                                                                                                     <  2:1618064/110‑1 (MQ=255)                                                                         aTCCGGGCGGTCAAGTCCGCCGATGACGAGAAACGCCTGCGTGTCGCGCGTGAAGTGTTCGACATCTACGTGCCGCTCGCGCACCGCCTGGGTATCGATCACATCAAGTGGGAGCTGGAAGACCTGTCCTTCCGCTACCTGGAGCCCGAc                                                            <  2:985969/150‑1 (MQ=255)                                                                           ccGGGCGGTCAAGTCCGCCGATGACGAGAAACGCCTGCGTGTCGCGCGTGAAGTGTTCGACATCTACGTGCCGCTCGCGCACCGCCTGGGTATCGATCACATCAAGTGGGAGCTGGAAGACCTGTCCTTCCGCTACCTGGAGCCCGACCa                                                          <  1:880568/150‑1 (MQ=255)                                                                             gggCGGTCAAGTCCGCCGATGACGAGAAACGCCTGCGTGTCGCGCGTGAAGTGTTCGACATCTACGTGCCGCTCGCGCACCGCCTGGGTATCGATCACATCAAGTGGGAGCTGGAAGACCTGTCCTTCCGCTACCt                                                                      <  2:230848/136‑1 (MQ=255)                                                                             gggCGGTCAAGTCCGCCGATGACGAGAAACGCCTGCGTGTCGCGCGTGAAGTGTTCGACATCTACGTGCCGCTCGCGCACCGCCTGGGTATCGATCACATCAAGTGGGAGCTGGAAGACCTGTCCTTCCGCTACCt                                                                      >  1:230848/1‑136 (MQ=255)                                                                              ggCGGTCAAGTCCGCCGATGACGAGAAACGCCTGCGTGTCGCGCGTGAAGTGTTCGACATCTACGTGCCGCTCGCGCACCGCCTGGGTATCGATCACATCAAGTGGGAGCTGGAAGACCTGTCCTTCCGCTACCTGGAGCCCGACCAGTa                                                       >  1:824733/1‑150 (MQ=255)                                                                               gCGGTCAAGTCCGCCGATGACGAGAAACGCCTGCGTGTCGCGCGTGAAGTGTTCGACATCTACGTGCCGCTCGCGCACCGCCTGGGTATCGATCACATCAAGTGGGAGCTGGAAGACCTGTCCTTCCGCTACCTGGAGCCCGACCAGTAc                                                      <  2:128032/150‑1 (MQ=255)                                                                                   tCAAGTCCGCCGATGACGAGAAACGCCTGCGTGTCGCGCGTGAAGTGTTCGACATCTACGTGCCGCTCGCGCACCGCCTGGGTATCGATCACATCAAGTGGGAGCTGGAAGACCTGTCCTTCCGCTACCTGGAGCCCGACCAGTACAAGc                                                  <  2:657885/150‑1 (MQ=255)                                                                                     aaGTCCGCCGATGACGAGAAACGCCTGCGTGTCGCGCGTGAAGTGTTCGACATCTACGTGCCGCTCGCGCACCg                                                                                                                            >  2:3153/1‑74 (MQ=255)                                                                                     aaGTCCGCCGATGACGAGAAACGCCTGCGTGTCGCGCGTGAAGTGTTCGACATCTACGTGCCGCTCGCGCACCg                                                                                                                            <  1:3153/74‑1 (MQ=255)                                                                                     aaGTCCGCCGATGACGAGAAACGCCTGCGTGTCGCGCGTGAAGTGTTCGACATCTACGTGCCGCTCGCGCACCGCCTGGGTATCGATCACATCAAGTGGGAGCTGGAAGACCTGTCCTTCCGCTACCTGGAGCCCGACCAGTACAAGCAg                                                >  1:795942/1‑150 (MQ=255)                                                                                      agcccgccgATGACGAGAAACGCCTGCGTGTCGCGCGTGAAGTGTTCGACATCTACGTGCCGCTCGCGCACCGCCTGGGTATCGATCACATCAAGTGGGAGCTGGAAGACCTGTCCTTCCGCTACCTGGAGCCCGACCAGTACAAGCAGa                                               <  1:435135/147‑1 (MQ=255)                                                                                      aGTCCGCCGATGACGAGAAACGCCTGCGTGTCGCGCGTGAAGTGTTCGACATCTACGTGCCGCTCGCGCACCGCCTGGGTATCGATCACATCAAGTGGGAGCTGGAAGACCTGTCCTTCCGCTACCTGGAGCCCGACCAGTACAAGCAGa                                               <  2:457409/150‑1 (MQ=255)                                                                                             taaTGACGAGAAACGCCTGCGTGTCGCGCGTGAAGTGTTCGACATCTACGTGCCGCTCGCGCACCGCCTGGGTATCGATCACATCAAGTGGGAGCTGGAAGACCTGTCCTTCCGCTACCTGGAGCCCGACCAGTACAAGCAGATCGCCaa                                        >  1:58497/3‑150 (MQ=255)                                                                                                         aCGCCTGCGTGTCGCGCGTGAAGTGTTCGACATCTACGTGCCGCTCGCGCACCGCCTGGGTATCGATCACATCAAGTGGGAGCTGGAAGACCTGTCCTTCCGCTACCt                                                                      <  2:1247557/108‑1 (MQ=255)                                                                                                         aCGCCTGCGTGTCGCGCGTGAAGTGTTCGACATCTACGTGCCGCTCGCGCACCGCCTGGGTATCGATCACATCAAGTGGGAGCTGGAAGACCTGTCCTTCCGCTACCt                                                                      >  1:1247557/1‑108 (MQ=255)                                                                                                         aCGCCTGCGTGTCGCGCGTGAAGTGTTCGACATCTACGTGCCGCTCGCGCACCGCCTGGGTATCGATCACATCAAGTGGGAGCTGGAAGACCTGTCCTTCCGCTACCTGGAGCCCGACCAGTACAAGCAGATCGCCAAGCTGTTGCATGa                            >  2:1105331/1‑150 (MQ=255)                                                                                                            ccTGCGTGTCGCGCGTGAAGTGTTCGACATCTACGTGCCGCTCGCGCACCGCCTGGGTATCGATCACATCAAGTGGGAGCTGGAAGACCTGTCCTTCCGCTACCTGGAGCCCGACCAGTACAAGCAGTTCGCCAAGCTGTTGCATGAgcg                         <  2:824733/150‑1 (MQ=255)                                                                                                              tGCGTGTCGCGCGTGAAGTGTTCGACATCTCCGTGCCGCTCGCGCACCGCCTGGGTATCGATCACATCAAGTGGGAGCTGGAAGACCTGTCCTTCCGCTACCTGGAGCCCGACCAGTACAAGCAGATCGCCAAGCTGTTGCATGAgcggc                       >  2:311873/1‑150 (MQ=255)                                                                                                                     cgcgcgTGAAGTGTTCGACATCTACGTGCCGCTCGCGCCCCGCCTCTGTATCTATCACATCAAGTGGGAGCTGGCAGACCTGTCCTTCCGCTACCTGGAGCCCGACCAGTACAAGCAGATCGCCAAGCTGTTGCATGAGCGGCGGCTGGa                <  1:373633/150‑1 (MQ=255)                                                                                                                            gAAGTGTTCGACATCTACGTGCCGCTCGCGCACCGCCTGGGTATCGATCACATCAAGTGGGAGCTGGAAGACCTGTCCTTCCGCTACCTGGAGCCCGACCAGTACAAGCAGATCGCCAAGCTGTTGCATGAGCGGCGGCTGGACCGCGAg         >  2:966777/1‑150 (MQ=255)                                                                                                                               gtgtTCGACATCTACGTGCCGCTCGCGCACCGCCTGGGTATCGATCACATCAAGTGGGAGCTGGAAGACCTGTCCTTCCGCTACCTGGAGCCCGACCAGTACAAGCAGATCGCCAAGCTGTTGCATGAGCGGCGGCTGGACCGCGAgcgc      <  1:1064875/150‑1 (MQ=255)                                                                                                                                  ttCGACATCTACGTGCCGCTCGCGCACCGCCTGGGTATCGATCACATCAAGTGGGAGCTGGAAGACCTGTCCTTCCGCTACCTGGAGCCCGACCAGTACAAGCAGATCGCCAAGCTGTTGCATGAGCGGCGGCTGGACCGCGAGCGCTtc   >  2:62151/1‑150 (MQ=255)                                                                                                                                   tCGACATCTACGTGCCGCTCGCGCACCGCCTGGGTATCGATCACATCAAGTGGGAGCTGGAAGACCTGTCCTTCCGCTACCTGGAGCCCGACCAGTACAAGCAGATCGCCAAGCTGTTGCATGAGCGGCGGCTGGACCGCGGGCGCTtca  >  1:71307/1‑150 (MQ=255)                                                                                                                                               |                                                                                                                                          CGCAAGATGCTGGTGGCCATGGTCGACGACGTGCGCGTGGCGCTGATCAAGCTGGCCGAACGCACGTGCGCAATCCGGGCGGTCAAGTCCGCCGATGACGAGAAACGCCTGCGTGTCGCGCGTGAAGTGTTCGACATCTACGCGCCGCTCGCGCACCGCCTGGGTATCGATCACATCAAGTGGGAGCTGGAAGACCTGTCCTTCCGCTACCTGGAGCCCGACCAGTACAAGCAGATCGCCAAGCTGTTGCATGAGCGGCGGCTGGACCGCGAGCGCTTCA  >  NC_002947/1851395‑1851674

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑