Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_002947 4,635,632 A→T M57K (ATG→AAG)  uvrY ← BarA/UvrY two‑componenent system response regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,635,6320AT98.2% 180.8 / NA 57M57K (ATG→AAG) uvrYBarA/UvrY two‑componenent system response regulator
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base T (30/26);  minor base G (1/0);  total (31/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ACCCTTGGTCAGGTAGCCGGCAGCGCCGGCCTGCAACAAGCGTGTGGGGAACGGGTCTTCCTCGCACACCGTCACCGCCACTACCTTGATGTCAGGGTGGCTGCGCAGCAGTTTGCGGGTGGCCTCCAGGCCGCCGATGCCGGGCATTTTCACATCCATCAGCACAACGTCCGGTTTCAGCTCCCGGGCCAGTTTCAGTGCCGACTCGCCGGAATCTCCCTCACCCACCACCTGCAGGCCGTCGATGTCGGCCAACATCCGTGTAATACCGGTTCGTACCAGATCGTGATC  >  NC_002947/4635487‑4635777
                                                                                                                                                 |                                                                                                                                                 
aCCCTTGGTCAGGTAGCCGGCAGCGCCGGCCTGCAACAAGCGTGTGGGGAACGGGTCTTCCTCGCACACCGTCACCGCCACTACCTTGATGTCAGGGTGGCTGCGCAGCAGTTTGCGGGTGGCCTCCAGGCCGCCGATGCCGGGCTtttt                                                                                                                                               <  1:22027/150‑1 (MQ=255)
  ccTTGGTCAGGTAGCCGGCAGCGCCGGCCTGCAACAAGCGTGTGGGGAACGGGTCTTCCTCGCACACCGTCACCGCCACTACCTTGATGTCGGGGTGGCTGCGCAGCAGTTTGCGGGTGGCCTCCAGGCCGCCGATGCCGGGCGTTTTca                                                                                                                                             >  1:406775/1‑150 (MQ=255)
    ttGGTCAGGTAGCCGGCAGCGCCGGCCTGCAACAAGCGTGTGGGGAACGGGTCTTCCTCGCACACCGTCACCGCCACTACCTTGATGTCAGGGTGGCTGCGCAGCAGTTTGCGGGTGGCCTCCAGGCCGCCGATGCCGGGCTTTTTcaca                                                                                                                                           >  2:30826/1‑150 (MQ=255)
    ttGGTCAGGTAGCCGGCAGCGCCGGCCTGCAACAAGCGTGTGGGGAACGGGTCTTCCTCGCACACCGTCACCGCCACTACCTTGATGTCAGGGTGGCTGCGCAGCAGTTTGCGGGTGGCCTCCAGGCCGCCGATGCCGGGCTTTTTcaca                                                                                                                                           >  1:212748/1‑150 (MQ=255)
           ggTAGCCGGCAGCGCCGGCCTGCAACAAGCGTGTGGGGAACGGGTCTTCCTCGCACACCGTCACCGCCACTACCTTGATGTCAGGGTGGCTGCGCAGCAGTTTGCGGGTGGCCTCCAGGCCGCCGATGCCGGGCTTTTTCACATCCATCa                                                                                                                                    >  1:139100/1‑150 (MQ=255)
                      gcgcCGGCCTGCAACAAGCGTGTGGGGAACGGGTCTTCCTCGCACACCGTCACCGCCACTACCTTGATGTCAGGGTGGCTGCGCAGCAGTTTGCGGGTGGCCTCCAGGCCGCCGATGCCGGGCTTTTTCACATCCATCAGCACAACGTcc                                                                                                                         <  2:212748/150‑1 (MQ=255)
                        gcCGGCCTGCAACAAGCGTGTGGGGAACGGGTCTTCCTCGCACACCGTCACCGCCACTACCTTGATGTCAGGGTGGCTGCGCAGCAGTTTGCCGTTTGCCTCCAGGCCGCCGATGCCGGGCTTTTTCACATCCATCAGCACAACGTCCgg                                                                                                                       >  1:870775/1‑150 (MQ=255)
                             ccTGCAACAAGCGTGTGGGGAACAGGTCTTCCTCGCACACCGTCACCGCCACTACCTTGATGTCAGGGTGGCTGCGCAGCAGTTTGCGGGTGGCCTCCAGGCCGCCGATGCCGGGCTTTTTCACATCCATCAGCACAACGTCCGGTTTCa                                                                                                                  <  2:560027/150‑1 (MQ=255)
                               tGCAACAAGCGTGTGGGGAACGGGTCTTCCTCGCACACCGTCACCGCCACTACCTTGATGTCAGGGTGGCTGCGCAGCAGTTTGCGGGTGGCCTCCAGGCCGCCGATGCCGGGCTTTTTCACATCCATCAGCACAACGTCCGGTTTCAGc                                                                                                                >  1:195517/1‑150 (MQ=255)
                                  aacaaGCGTGTGGGGAACGGGTCTTCCTCGCACACCGTCACCGCCACTACCTTGATGTCAGGGTGGCTGCGCAGCAGTTTGCGGGTGGCCTCCAGGCCGCCGATGCCGGGCTTTTTCACATCCATCAGCACAACGTCCGGTTTCAGCTcc                                                                                                             >  1:527611/1‑150 (MQ=255)
                                    caaGCGTGTGGGGAACGGGTCTTCCTCGCACACCGTCACCGCCACTACCTTGATGTCAGGGTGGCTGCGCAGCAGTTTGCGGGTGGCCTCCAGGCCGCCGATGCCGGGCttt                                                                                                                                                 <  2:563917/112‑1 (MQ=255)
                                         gtgtGGGGAACGGGTCTTCCTCGCACACCGTCACCGCCACTACCTTGATGTCAGGGTGGCTGCGCAGCAGTTTGCGGGTGGCCTCCAGGCCGCCGATGCCGGGCTTTTTCACATCCATCAGCACAACGTCCGGTTTCAGCTCCCGGGCCa                                                                                                      <  2:195517/150‑1 (MQ=255)
                                              gggAACGGGTCTTCCTCGCACACCGTCACCGCCACTACCTTGATGTCAGGGTGGCTGCGCAGCAGTTTGCGGGTGGCCTCCAGGCCGCCGATGCCGGGCTTTTTCACATCCATCAGCACAACGTCCGGTTTCAGCTCCCGGGCCAGTTTc                                                                                                 >  2:1482179/1‑150 (MQ=255)
                                                gAACGGGTCTTCCTCGCACACCGTCACCGCCACTACCTTGATGTCAGGGTGGCTGCGCAGCAGTTTGCGGGTGGCCTCCAGGCCGCCGATGCCGGGCTTTTTCACATCCATCAGCACAACGTCCGGTTTCAGCTCCCGGGCCAGTTTCAg                                                                                               <  2:246280/150‑1 (MQ=255)
                                                gAACGGGTCTTCCTCGCACACCGTCACCGCCACTACCTTGATGTCAGGGTGGCTGCGCAGCAGTTTGCGGGTGGCCTCCAGGCCGCCGATGCCGGGCTTTTTCACATCCATCAGCACAACGTCCGGTTTCAGCTCCCGGGCCAGTTTCAg                                                                                               >  2:889200/1‑150 (MQ=255)
                                                 aaCGGGTCTTCCTCGCACCCCGTCACCGCCACTACCTTGATGTCAGGGTGGCTGCGCAGCAGTTTGCCGACACCCTCCAGGCCGCCGATGCCGGGCTTTTTCACATCCATCAGCACAACGTCCGGTTTCAGCTCCCGGGCCAGTTTCAGt                                                                                              <  1:30826/150‑1 (MQ=255)
                                                 aaCGGGTCTTCCTCGCACACCGTCACCGCCACTACCTTGATGTCAGGGTGGCTGCGCAGCAGTTTGCGGGTGGCCTCCAGGCCGCCGATGCCGGGCTTTTTCACATCCCACAGCCCTACGTCCGTTGTCAGCTCCCGGGCCAGTTTCAGt                                                                                              <  1:889200/150‑1 (MQ=255)
                                                        cTTCCTCGCACACCGTCACCGCCACTACCTTGATGTCAGGGTGGCTGCGCAGCAGTTTGCGGGTGGCCTCCAGGCCGCCGATGCCGGGCTTTTTCACATCCATCAGCACAACGTCCGGTTTCAGCTCCCGGGCCAGTTTCAGTGCCGACt                                                                                       <  2:1352055/150‑1 (MQ=255)
                                                         ttCCTCGCACACCGTCACCGCCACTACCTTGATGTCAGGGTGGCTGCGCAGCAGTTTGCGGGTGGCCTCCAGGCCGCCGATGCCGGGCTTTTTCACATCCATCAGCACAACGTCCGGTTTCAGCTCCCGGGCCAGTTGCAGTGCCGACTc                                                                                      >  1:775892/1‑150 (MQ=255)
                                                         ttCCTCGCACACCGTCACCGCCACTACCTTGATGTCAGCATCGCCGTCTAGACGTCTGTGGGTGGCCTCCTGGCCGCCGATGCCGGGCTTTTTCACATCGATCAGCACAACGTCCTGTTTCAGCTCCCGGGCCAGTTTCAGTGCCGACTc                                                                                      >  1:31963/1‑150 (MQ=255)
                                                             tCGCACACCGTCACCGCCACTACCTTGATGTCGGGGTGGCTGCGCAGCAGTTTGCGGGTGGCCTCCAGGCCGCCGATGCCGGGCTTTTTCACATCCATCAGCACAACGTCCGGTTTCAGCTCCCGGGCCAGTTTCAGTGCCGACTCGCCg                                                                                  <  2:406775/150‑1 (MQ=255)
                                                             tCGCACACCGTCACCGCCACTACCTTGATGTCAGGGTGGCTGCGCAGCAGTTTGCGGGTGGCCTCCAGGCCGCCGATGCCGGGCTTTTTCACATCCATCa                                                                                                                                    <  1:1005740/100‑1 (MQ=255)
                                                             tCGCACACCGTCACCGCCACTACCTTGATGTCAGGGTGGCTGCGCAGCAGTTTGCGGGTGGCCTCCAGGCCGCCGATGCCGGGCTTTTTCACATCCATCa                                                                                                                                    >  2:1005740/1‑100 (MQ=255)
                                                             tCGCACACCGTCACCGCCACTACCTTGATGTCAGGGTGGCTGCGCAGCAGTTTGCGGGTGGCCTCCAGGCCGCCGATGCCGGGCTTTTTCACATCCATCAGCACAACGTCCGGTTTCAGCTCCCGGGCCAGTTTCAGTGCCGACTCGCCg                                                                                  >  2:263265/1‑150 (MQ=255)
                                                                cacaCCGTCACCGCCACTACCTTGATGTCAGGGTGGCTGCGCAGCAGTTTGCGGGTGGCCTCCAGGCCGCCGATGCCGGGCTTTTTCACATCCATCAGCACAACGTCCGGTTTCAGCTCCCGGGCCAGTTTCAGTGCCGACTCGCCGGaa                                                                               <  1:263265/150‑1 (MQ=255)
                                                                                    cTTGATGTCAGGGTGGCTGCGCAGCAGTTTGCGGGTGGCCTCCAGGCCGCCGATGCCGGGCTTTTTCACATCCATCAGCACAACGTCCGGTTTCAGCTCCCGGGCCAGTTTCAGTTCCGACTCGCCGGGATCTCCCTCACCCACCACCtc                                                           >  2:300886/1‑149 (MQ=255)
                                                                                     ttGATGTCAGGGTGGCTGCGCAGCAGTTTGCGGGTGGCCTCCAGGCCGCCGATGCCGGGCTTTTTCACATCCATCAGCACAACGTCCGGTTTCAGCTCCCGGGCCAGTTTCAGTGCCGa                                                                                         <  2:1665597/119‑1 (MQ=255)
                                                                                     ttGATGTCAGGGTGGCTGCGCAGCAGTTTGCGGGTGGCCTCCAGGCCGCCGATGCCGGGCTTTTTCACATCCATCAGCACAACGTCCGGTTTCAGCTCCCGGGCCAGTTTCAGTGCCGa                                                                                         >  1:1665597/1‑119 (MQ=255)
                                                                                          gTCAGGGTGGCTGCGCAGCAGTTTGCGGGTGGCCTCCAGGCCGCCGATGCCGGGCTTTTTCACATCCATCAGCACAACGTCCGGTTTCAGCTCCCGGGCCAGTTTCAGTGCCGACTCGCCGGAATCTCACTCGCCCACCACCTGCAGGcc                                                     >  1:88654/1‑150 (MQ=255)
                                                                                           tCAGGGTGGCTGCGCAGCAGTTTGCGGGTGGCCTCCAGGCCGCCGATGCCGGGCTTTTTCACATCCATCAGCACAACGTCCGGTTTCAGCTCCCGGGCCAGTTTCAGTGCCGACTCGCCGGAATCTCCCTCACCCACCACCTGCAGGCCg                                                    >  2:922889/1‑150 (MQ=255)
                                                                                                     tGCGCAGCAGTTTGCGGGTGGCCTCCAGGCCGCCGATGCCGGGCTTTTTCACATCCATCAGCACAACGTCCGGTTTCAGCTCCCGGGCCAGTTTCAGTGCCGACTCGCCg                                                                                  >  1:134290/1‑110 (MQ=255)
                                                                                                     tGCGCAGCAGTTTGCGGGTGGCCTCCAGGCCGCCGATGCCGGGCTTTTTCACATCCATCAGCACAACGTCCGGTTTCAGCTCCCGGGCCAGTTTCAGTGCCGACTCGCCg                                                                                  <  2:134290/110‑1 (MQ=255)
                                                                                                         cagcagTTTGCGGGTGGCCTCCAGGCCGCCGATGCCGGGCTTTTTCACATCCATCAGCACAACGTCCGGTTTCAGCTCCCGGGc                                                                                                        <  1:110027/84‑1 (MQ=255)
                                                                                                         cagcagTTTGCGGGTGGCCTCCAGGCCGCCGATGCCGGGCTTTTTCACATCCATCAGCACAACGTCCGGTTTCAGCTCCCGGGc                                                                                                        >  2:110027/1‑84 (MQ=255)
                                                                                                          agcagTTTGCGGGTGGCCTCCAGGCCGCCGATGCCGGGCTTTTTCACATCCATCAGCATAACGTCCGGTTTCAGCTCCCGGGCCAGTTTCAGTGCCGACTCGCCGGAATCTCCCTCACCCACCACCTGCAGGCCGTCGATGTCGGCCAAc                                     >  1:498715/1‑150 (MQ=255)
                                                                                                          agcagTTTGCGGGTGGCCTCCAGGCCGCCGATGCCGGGCTTTTTCACATCCATCAGCACAACGTCCGGTTTCAGCTCCCGGGCCAGTTTCAGTGCCGACTCGCCGGAATCTCCCTCACccacca                                                               >  1:238047/1‑124 (MQ=255)
                                                                                                          agcagTTTGCGGGTGGCCTCCAGGCCGCCGATGCCGGGCTTTTTCACATCCATCAGCACAACGTCCGGTTTCAGCTCCCGGGCCAGTTTCAGTGCCGACTCGCCGGAATCTCCCTCACccacca                                                               <  2:238047/124‑1 (MQ=255)
                                                                                                               tttGCGGGTGGCCTCCAGGCCGCCGATGCCGGGCTTTTTCACATCCATCAGCACAACGTCCGGTTTCAGCTCCCGGGCCAGTTTCAGTGCCGACTCGCCgg                                                                                 <  2:676145/101‑1 (MQ=255)
                                                                                                               tttGCGGGTGGCCTCCAGGCCGCCGATGCCGGGCTTTTTCACATCCATCAGCACAACGTCCGGTTTCAGCTCCCGGGCCAGTTTCAGTGCCGACTCGCCgg                                                                                 >  1:676145/1‑101 (MQ=255)
                                                                                                                    gggTGGCCTCCAGGCCGCCGATGCCGGGCTTTTTCACATCCATCAGCACAACGTCCGGTTTCAGCTCCCGGGCCAGTTTCAGTGCCGACTCGCCGGAATCTCCCTCACCCACCACCTGCAGGCCGTCGAt                                               >  1:1053562/1‑130 (MQ=255)
                                                                                                                    gggTGGCCTCCAGGCCGCCGATGCCGGGCTTTTTCACATCCATCAGCACAACGTCCGGTTTCAGCTCCCGGGCCAGTTTCAGTGCCGACTCGCCGGAATCTCCCTCACCCACCACCTGCAGGCCGTCGAt                                               <  2:1053562/130‑1 (MQ=255)
                                                                                                                        ggCCTCCAGGCCGCCGATGCCGGGCTTTTTCACATCCATCAGCACAACGTCCGGTTTCAGCTCCCGGGCCAGTTTCAGTGCCGACTCGCCGGAATCTCCCTCACCCACCACCTGCAGGCCGTCGATGTCGGCCAACATCCGTGTAATAcc                       <  2:966012/150‑1 (MQ=255)
                                                                                                                           cTCCAGGCCGCCTATGCCGGGCTTTTTCACATCCATCAGCACAACGTCCGGTTTCAGCTCCCGGGCCAGTTTCAGTGCCGACTCGCCGGAATCTCCCTCACCCACCACCTGCAGGCCGTCGATGTCGGCCAACATCCGTGTAATACCGGt                    <  2:527611/150‑1 (MQ=255)
                                                                                                                           cTCCAGGCCGCCGATGCCGGGCTTTTTCACATCCATCAGCACAACGTCCGGTTTCAGCTCCCGGGCCAGTTTCAGTGCCGACTCGCCGGAATCTCCCTCACCCACCACCTGCAGGCCGTCGATGTCGGCCAACATCCGTGTAATACCGGt                    >  2:190964/1‑150 (MQ=255)
                                                                                                                           cTCCAGGCCGCCGATGCCGGGCTTTTTCACATCCATCAGCACAACGTCCGGTTTCAGCTCCCGGGCCAGTTTCAGTGCCGACTCGCCGGAATCTCCCTCACCCACCACCTGCAGGCCGTCGATGTCGGCCAACATCCGTGTAATACCGGt                    >  1:849709/1‑150 (MQ=255)
                                                                                                                               aGGCCGCCGATGCCGGGCTTTTTCACATCCATCAGCACAACGTCCGGTTTCAGCTCCCGGGCCAGTTTCAGTGCCGACTCGCCGGAATCTCCCTCACCCACCACCTGCAGGCCGTCGATGTCGGCCAACATCCgtg                              >  2:1602656/1‑136 (MQ=255)
                                                                                                                               aGGCCGCCGATGCCGGGCTTTTTCACATCCATCAGCACAACGTCCGGTTTCAGCTCCCGGGCCAGTTTCAGTGCCGACTCGCCGGAATCTCCCTCACCCACCACCTGCAGGCCGTCGATGTCGGCCAACATCCgtg                              <  1:1602656/136‑1 (MQ=255)
                                                                                                                                       gATGCCGGGCTTTTTCACATCCATCAGCATAACGTCCGGTTTCAGCTCCCGGGCCAGTTTCAGTGCCGACTCGCCGGAATCTCCCTCACCCACCACCTGCAGGCCGTCGATGTCGGCCAACATCCGTGTAATACCGGTTCGTACCAGATc        <  2:498715/150‑1 (MQ=255)
                                                                                                                                           ccGGGCTTTTTCACATCCATCAGCACAACGTCCGGTTTCAGCCCCCGGGCCAGTTTCAGTGCCGACTCGCCGGAATCTCCCTCACCCACCACCTGCAGGCCGTCGATGTCGGCCAACATCCGTGTAATACCGGTTCGTACCAGATCGTGa    <  2:139100/150‑1 (MQ=255)
                                                                                                                                            cGGGCTTTTTCACATCCATCAGCACAACGTCCGGTTTCAGCTCCCGGGCCAGTTTCAGTGCCGACTCGCCGGAATCTCCCTCACCCACCACCTGCAGGCCGTCGATGTCGGCCAACATCCGTGTAATAc                        >  1:770485/1‑129 (MQ=255)
                                                                                                                                            cGGGCTTTTTCACATCCATCAGCACAACGTCCGGTTTCAGCTCCCGGGCCAGTTTCAGTGCCGACTCGCCGGAATCTCCCTCACCCACCACCTGCAGGCCGTCGATGTCGGCCAACATCCGTGTAATAc                        <  2:283713/129‑1 (MQ=255)
                                                                                                                                            cGGGCTTTTTCACATCCATCAGCACAACGTCCGGTTTCAGCTCCCGGGCCAGTTTCAGTGCCGACTCGCCGGAATCTCCCTCACCCACCACCTGCAGGCCGTCGATGTCGGCCAACATCCGTGTAATAc                        >  1:283713/1‑129 (MQ=255)
                                                                                                                                            cGGGCTTTTTCACATCCATCAGCACAACGTCCGGTTTCAGCTCCCGGGCCAGTTTCAGTGCCGACTCGCCGGAATCTCCCTCACCCACCACCTGCAGGCCGTCGATGTCGGCCAACATCCGTGTAATAc                        <  2:770485/129‑1 (MQ=255)
                                                                                                                                             gggCTTTTTCACATCCATCAGCACAACGTCCGGTTTCAGCTCCCGGGCCAGTTTCAGTGCCGACTCGCCGGAATCTcc                                                                          <  2:406963/78‑1 (MQ=255)
                                                                                                                                             gggCTTTTTCACATCCATCAGCACAACGTCCGGTTTCAGCTCCCGGGCCAGTTTCAGTGCCGACTCGCCGGAATCTcc                                                                          >  1:406963/1‑78 (MQ=255)
                                                                                                                                             gggCTTTTTCACATCCATCAGCACAACGTCCGGTTTCAGCTCCCGGGCCAGTTTCAGTGCCGACTCGCCGGAATCTCCCTCACCcaccac                                                              <  2:1212747/90‑1 (MQ=255)
                                                                                                                                             gggCTTTTTCACATCCATCAGCACAACGTCCGGTTTCAGCTCCCGGGCCAGTTTCAGTGCCGACTCGCCGGAATCTCCCTCACCcaccac                                                              >  1:1212747/1‑90 (MQ=255)
                                                                                                                                             gggCTTTTTCACATCCATCAGCACAACGTCCGGTTTCAGCTCCCGGGCCAGTTTCAGTGCCGACTCGCCGGAATCTCCCTCACCCACCACCTGCAGGCCGTCGATGTCGGCCAACATCCGTGTAATACCGGTTCGTACCAGATCGTGatc  >  1:424737/1‑150 (MQ=255)
                                                                                                                                                 |                                                                                                                                                 
ACCCTTGGTCAGGTAGCCGGCAGCGCCGGCCTGCAACAAGCGTGTGGGGAACGGGTCTTCCTCGCACACCGTCACCGCCACTACCTTGATGTCAGGGTGGCTGCGCAGCAGTTTGCGGGTGGCCTCCAGGCCGCCGATGCCGGGCATTTTCACATCCATCAGCACAACGTCCGGTTTCAGCTCCCGGGCCAGTTTCAGTGCCGACTCGCCGGAATCTCCCTCACCCACCACCTGCAGGCCGTCGATGTCGGCCAACATCCGTGTAATACCGGTTCGTACCAGATCGTGATC  >  NC_002947/4635487‑4635777

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑