Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	4,741,298:1	+T	53.8%	intergenic (+489/+263)	gltA → / ← yeiW	citrate synthase/putative oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	4,741,298	1	.	T	53.8%	18.9 / 17.7	13	intergenic (+489/+263)	gltA/yeiW	citrate synthase/putative oxidoreductase
Reads supporting (aligned to +/- strand):  ref base . (2/4);  new base T (3/4);  total (5/8)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TCAGGCTGCCATCCGCCTCCTTGATGGTCGTAATGTGCAGCACGGAATCACGCGGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAG‑AA‑CCTCC‑GCCAAC‑TGCATTCGTGAA‑CCAAGCAACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCAC  >  NC_002947/4741156‑4741404                                                                                                                                                    |                                                                                                           tCAGGCTGCCATCCGCCTCCTTGATGGTCGTAATGTTCAGCACGGAATCCCGCGGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGTTCACCTTCGTGCTTGAGCAG‑CTTCT‑GCGACT‑TGCATTCGTGaatccagg                                                                                                       >  2:38604/1‑144 (MQ=255)                    ctTGATGGTCGTAATGTGCAGCACGGAATCACGCGGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCCCTTGTCACCTTCGTGCTTGCGCAG‑GCCAC‑GCAGCA‑TGCAGTCGGGAA‑TCCAGGCACAGGCCCCGGACACGtc                                                                                   >  1:494678/1‑150 (MQ=255)                       gATGGTCGTAATGTGCAGCACGGAATCACGCGGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAG‑CTTCT‑GCGACT‑TGCATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGtcctc                                                                                <  1:256443/150‑1 (MQ=255)                                      cagcaCGGAATCACGCGGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAG‑CAGCTTCT‑GCGACT‑TGCATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGAc                                                                 <  2:23743/150‑1 (MQ=255)                                       agcaCGGAATCACGCGGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAG‑CTTCT‑GCGACT‑TGCATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGAcc                                                                <  1:45481/150‑1 (MQ=255)                                           cGGAATCACGCGGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAG‑TAGCTTCT‑GCGACT‑TGCATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTgg                                                            >  1:262173/1‑150 (MQ=255)                                                        cgTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGTTCACCTTCGTGCTTGAG‑CAGCTTCT‑GCGACT‑TGCATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCtt                                               <  1:38604/150‑1 (MQ=255)                                                            aCCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAG‑CTTCT‑GCGACT‑TGCATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATc                                           >  2:151220/1‑150 (MQ=255)                                                                 ccATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGTAG‑CTTCT‑GCGACT‑TGCATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGt                                      <  2:262173/150‑1 (MQ=255)                                                                   ttGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAG‑CTTCT‑GCGACT‑TGCATTCGTGAA‑GCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCGCGTCCTTGGCCAGCGGCGCCTTGATCACGGTca                                    <  2:317587/149‑1 (MQ=255)                                                                                   ggTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAG‑CTTCT‑GCGACT‑TGCATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCtt                    <  2:494678/150‑1 (MQ=255)                                                                                             cAGCTCTGGTCACCTTCGTGCTTGAGCAG‑CTTCT‑GCGACT‑TGCATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGCCCTTATCCTTGCAc          >  2:28746/1‑150 (MQ=255)                                                                                              aGCTCTGGTCACCTTCGTGCTTGAG‑CA‑GCTTC‑TGCGACTTGCATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGAcc         >  1:253162/1‑150 (MQ=255)                                                                                                     gTCACCTTCGTGCTTGAG‑CA‑GCTTCTGCGACT‑TGCATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCAc  <  1:151220/150‑1 (MQ=255)                                                                                                                                                    |                                                                                                           TCAGGCTGCCATCCGCCTCCTTGATGGTCGTAATGTGCAGCACGGAATCACGCGGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAG‑AA‑CCTCC‑GCCAAC‑TGCATTCGTGAA‑CCAAGCAACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCAC  >  NC_002947/4741156‑4741404

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑