| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 3,344,389 | C→G | 23.6% | Q261H (CAG→CAC) | PP_2941 ← | hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 3,344,389 | 0 | C | G | 23.6% | 27.0 / 7.4 | 17 | Q261H (CAG→CAC) | PP_2941 | hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base C (7/6); new base G (2/2); total (9/8) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.65e-01 | |||||||||||
GCCATTCTCGAAGGGAAGGTTATGGCTGAACAGTACGTGGAAAGTCAGAAAAGCGGAGCTTGTGCTGCCAGGTGAGTTCGCCGAGGTCTGATCGGACCCACCGCAACCACGGGCAGGTCGACCACCTGCGAATCTGTGCAGCCGCTTCACTGAAGCGGCTCTATCAGCTCAGCGCCTTGATTGCGAACATTGGCAATGGCTGTGCTCACTCGATACCACTCGAAGGCCTCGGCTGGCTCGCCCAGGTTGAGCGCCATCTGCTCGGCTTGTT > NC_002947/3344240‑3344510 | gCCATTCTCGAAGGGAAGGTTATGGCTGAACAGTACGTGGAAAGTCAGAAAAGCGGAGCTTGTGCTGCCAGGTGAGTTCGCCGAGGTCTGATCGGACCCACCGCAACCACGGGCAGGTCGACCACCTGCGAATCTGTGCAGCCGCTTCAc < 2:38337/150‑1 (MQ=255) aaaaGCGGAGCTTGTTCTGCCAGGTGAGTGCGCCGAGGTCTCATCGGACCCACCGCACCCACGGGCAGGTCGACCACCTGCGAATCTGTGCAGCCGCTTCACTGAAGCGGCTCTATCAGCTCAGCGCCTTGATTGCGAACATTGGCAATg < 2:221504/150‑1 (MQ=255) gAGCTTGTGCTGCCAGGTGAGTTCGCCGAGGTCTGATCGGACCCACCGCAACCACGGGCAGGTCGACCACCTGCGACTCTGTGCCTCCGCTTCACTGAAGCGGTTCTATCACCTCACCGCTTTGATTGCGAACATTGGCAATGGCTGTGc < 2:313034/150‑1 (MQ=255) cAGGTGAGTTCGCCGAGGTCTGATCGGACCCACCGCAACCACGGGCAGGTCGACCACCTGCGAATCTGTGCAGCCGCTTCACTGAAGCGGCTCTATCAGCTCAGCGCCTTGATTGCGat < 1:399947/119‑2 (MQ=255) cAGGTGAGTTCGCCGAGGTCTGATCGGACCCACCGCAACCACGGGCAGGTCGACCACCTGCGAATCTGTGCAGCCGCTTCACTGAAGCGGCTCTATCAGCTCAGCGCCTTGATTGCGaa > 2:399947/1‑119 (MQ=255) ttCGCCGAGGTCTGATCGGACCCACCGCAACCACGGGCAGGTCGACCACCTGCGAATCTGTGCAGCCGCTTCACTGAAGCgg < 1:23181/82‑1 (MQ=255) ttCGCCGAGGTCTGATCGGACCCACCGCAACCACGGGCAGGTCGACCACCTGCGAATCTGTGCAGCCGCTTCACTGAAGCgg > 2:23181/1‑82 (MQ=255) ccGAGGTCTGATCGGACCCACCGCAACCACGGGCAGGTCGACCAGGTTTTAATCTGTGCAGCCGCTTCACTGAAGCGGCTCTATCAGCTCAGCGCCTTGATTGCGAACATTGGCAATGGCTGTGCTCACTCGATACCACTCGAAGGCCTc > 1:118059/1‑150 (MQ=255) gACCCACCGCAACCACGGGCAGGTCGACCACCTGCGAATCTGTGCAGCCGCTTCACTGAAGCGGCTCTATCAGCTCAGCGCCTTGACTGCGAACATTGGCAATGGCTGTGCTCACTCGATACCa > 2:37008/1‑124 (MQ=255) gACCCACCGCAACCACGGGCAGGTCGACCACCTGCGAATCTGTGCAGCCGCTTCACTGAAGCGGCTCTATCAGCTCAGCGCCTTGACTGCGAACATTGGCAATGGCTGTGCTCACTCGATACCa < 1:37008/124‑1 (MQ=255) ccACGGGCAGGTCGACCACCTGCGAATCTGTGCAGCCGCTTCACTGAAGCGGCTCTATCAGCTCAGCGCCTTGATTGCGAACATTGGCAATGGCTGTGCTCACTCGATACCACTCGAAGGCCTCGGCTGGCTCGCCCAGGTTGAGCGCCa > 2:188490/1‑150 (MQ=255) ccACGGGCAGGTCGACCACCTGCGAATATGCGCAGCCGCTTCACTGAAGCGGCTCTATCAGCTCAGCGCCTTGATTGCGAACATTGGCAATGGCTGTGCTCACTCGATACCACTCGAAGGCCTCGGCTGGCTCGCCCAGGTTGAGCGCCa > 1:13550/1‑150 (MQ=255) gTCGACCACCTGCGAATCTGTGCAGCCGCTTCACTGAAGCGGCTCTATCAGCTCAGCGCCTTGATTGCGAACATTGGCAATGGCTGTGCTCACTCGATa > 2:15210/1‑99 (MQ=255) gTCGACCACCTGCGAATCTGTGCAGCCGCTTCACTGAAGCGGCTCTATCAGCTCAGCGCCTTGATTGCGAACATTGGCAATGGCTGTGCTCACTCGATa < 1:15210/99‑1 (MQ=255) gCCGCTTCAGTGAAGCGGCTCTATCAGCTCAGCGCCTTGATTGCGAACATTGGCAATGGCtg > 2:267081/1‑62 (MQ=255) gCCGCTTCAGTGAAGCGGCTCTATCAGCTCAGCGCCTTGATTGCGAACATTGGCAATGGCtg < 1:267081/62‑1 (MQ=255) gCCGCTTCAGTGAAGCGGCTCTATCAGCTCAGCGCCTTGATTGCGAACATTGGCAATGGCTGTGCTCACTCGATACCACTCGAAGGCCTCGGCTGGCTCGCCCAGGTTGAGCGCCATCTGCTCGGCTTGtt < 2:272898/131‑1 (MQ=255) gCCGCTTCAGTGAAGCGGCTCTATCAGCTCAGCGCCTTGATTGCGAACATTGGCAATGGCTGTGCTCACTCGATACCACTCGAAGGCCTCGGCTGGCTCGCCCAGGTTGAGCGCCATCTGCTCGGCTTGtt > 1:272898/1‑131 (MQ=255) | GCCATTCTCGAAGGGAAGGTTATGGCTGAACAGTACGTGGAAAGTCAGAAAAGCGGAGCTTGTGCTGCCAGGTGAGTTCGCCGAGGTCTGATCGGACCCACCGCAACCACGGGCAGGTCGACCACCTGCGAATCTGTGCAGCCGCTTCACTGAAGCGGCTCTATCAGCTCAGCGCCTTGATTGCGAACATTGGCAATGGCTGTGCTCACTCGATACCACTCGAAGGCCTCGGCTGGCTCGCCCAGGTTGAGCGCCATCTGCTCGGCTTGTT > NC_002947/3344240‑3344510 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |