| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 3,423,575 | C→G | 14.3% | P564A (CCA→GCA) | PP_3038 → | TraC domain‑containing protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 3,423,575 | 0 | C | G | 14.3% | 73.3 / 6.5 | 28 | P564A (CCA→GCA) | PP_3038 | TraC domain‑containing protein |
| Reads supporting (aligned to +/- strand): ref base C (12/12); new base G (2/2); total (14/14) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.98e-01 | |||||||||||
TTCCAAGCCATCCGAGGTAAGTGGATCGTCGAGCTGGGCGAATTGGACAGCTTCAACAAGGCAGAGAGCACCAAGGCCAAGCAATTCTTCTCGGCATCTATCGACACTTACCGCGAAAGCTATGGGCGAAGAACGAGTGACGTGCCACGTCAGTGTGTTTTCGCGGGTACCACCAACCAGGATGAATACCTTAAGGACGCCACCGGTAACCGGCGTTATTGGCCGGTCGCTTGCGTCAAGGTGGACCTTGAAGCATTGCGTCGCGCTCGTGACCAGCTGTGGGCTGAGGCCAT > NC_002947/3423431‑3423723 | ttCCAAGCCATCCGAGGTAAGTGGATCGTCGAGCTGGGCGAATTGGACAGCTTCAACAAGGCAGAGAGCACCAAGGCCAAGCAATTCTTCTCGGCATCTATCGACACTTACCGCGAAAGCTATGGGCGAAGAACGAGTGACGTGGCACGt < 1:368990/150‑1 (MQ=255) ccGAGGTAAGTGGATCGTCGAGCTGGGCGAATTGGACAGCTTCAACAAGGCAGAGAGCACCAAGGCCAAGCAATTCTTCTCGGCATCTATCGACACTTACCGCGAAAGCTATGGGCGAAGAACGAGTGACGTGCCACGTCAGTGTGtttt > 1:358921/1‑150 (MQ=255) tGGGCGAATTGGACAGCTTCAACAAGGCAGAGAGCACCAAGGCCAAGCAATTCTTCTCGGCATCTATCGACACTTACCGCGAAAGCTATGGGCGAAGAACGAGTGACGTGCCACGTCAGTGTGTTTTCGCGGGTACCACCAACCAGGATg < 1:82496/150‑1 (MQ=255) gggCGAATTGGACAGCTTCAACAAGGCAGAGAGCACCAAGGCCAAGCAATTCTTCTCGGCATCTATCGACACTTACCGCGAAAGCTATGGGCGAAGAACGAGTGACGTGCCCAGTCAGTGTGTATTGGCGGGTACCACCAACCAGGATGa > 2:65102/1‑150 (MQ=255) gAATTGGACAGCTTCAACAAGGCAGAGAGCACCAAGGCCAAGCAATTCTTCTCGGCATCTATCGACACTTACCGCGAAAGCTATGGGCGAAGAACGAGTGACGTGCCACGTCAGTGTGTTTTCGCGGGTACCACCAACCAGGATGAATAc < 1:46100/150‑1 (MQ=255) aaTTGGACAGCTTCAACAAGGCAGAGAGCACCAAGGCCAAGCAATTCTTCTCGGCATCTATCGACACTTACCGCGAAAGCTATGGGGGAAGAACGAGTGACGTGCCACGTCAGTGTGTTTTCGCGGGTACCACCAACCAGGATGAATAcc > 1:316221/1‑150 (MQ=255) cTTCAACAAGGCAGAGAGCACCAAGGCCAAGCAATTCTTCTCGGCATCTATCGACACTTACCGCGAAAGCTATGGGCGAAGAACGAGTGACGTGCCACGTCAGTGTGTTTTCGCGGGTACCACCAACCAGGATGAATACCTTAAGGACGc < 2:358921/150‑1 (MQ=255) acaaGGCAGAGAGCACCAAGGCCAAGCAATTCTTCTCGGCATCTATCGACACTTACCGCGAAAGCTATGGGCGAAGAACGAGTGACGTGCCACGTCAGTGTGTTTTCGCGGGTACCACCAACCAGGATGAATACCTTAAGGACGCCACCg > 2:344979/1‑150 (MQ=255) aaGGCAGAGAGCACCAAGGCCAAGCAATTCTTCTCGGCATCTATCGACACTTACCGCGAAAGCTATGGGCGAAGAACGAGTGACGTGCCACGTCAGTGTGTTTTCGCGGGTACCACCAACCAGGATGAATACCTTAAGGACGCCACCGGt > 1:14176/1‑150 (MQ=255) agagagCACCAAGGCCAAGCAATTCTTCTCGGCATCTATCGACACTTACCGCGAAAGCTATGGGCGAAGAACGAGTGACGTGCCACGTCAGTGTGTTTTCGCGGGTACCACCAACCAGGATGAATACCTTAAGGACGCCACCGGTAACCg < 2:12255/150‑1 (MQ=255) agCACCAAGGCCAAGCAATTCTTCTCGGCATCTATCGACACTTACCGCGAAAGCTATGGGCGAAGAACGAGTGACGTGCCACGTCAGTGTGTTTTCGCGGGTACCACCAACCAGGATGAATACCTTAAGGACGCCACCGGTAACCGGCGt < 2:456112/150‑1 (MQ=255) ccAAGGCCAAGCAATTCTTCTCGGCATCTATCGACACTTACCGCGAAAGCTATGGGCGAAGAACGAGTGACGTGCCACGTCAGTGTGTTTTCGCGGGTACCACCAACCAGGATGAATACCTTAAGGACGCCACCGGTAACCGGCGTTAtt < 2:2530/150‑1 (MQ=255) gCCAAGCAATTCTTCTCGGCATCTATCGACACTTACCGCGAAAGCTATGGGCGAAGAACGAGTGACGTGCCACGTCAGTGTGTTTTCGCGGGTACCACCAACCAGGATGAATACCTTAAGGACGCCACCGGTAACCGGCGTTATTGGCCg < 2:222626/150‑1 (MQ=255) aaTTCTTCTCGGCATCTATCGACACTTACCGCGAAAGCTATGGGCGAAGAACGAGTGACGTGCCACGTCAGTGTGTTTTCGCGGGTACCACCAACCAGGATGAATACCTTAAGGACGCCACCGGTAACCGGc > 1:452524/1‑132 (MQ=255) aaTTCTTCTCGGCATCTATCGACACTTACCGCGAAAGCTATGGGCGAAGAACGAGTGACGTGCCACGTCAGTGTGTTTTCGCGGGTACCACCAACCAGGATGAATACCTTAAGGACGCCACCGGTAACCGGc < 2:452524/132‑1 (MQ=255) tcttctCGGCATCTATCGACACTTACCGCGAAAGCTATGGGCGAAGAACGAGTGACGTGCCACGTCAGTGTGTTTTCGCGGGTACCACCAACCAGGATGAATACCTTAAGGACGCCACCGGTAACCGGCGTTATTGGCCGGTCGCTTGCg > 1:348107/1‑150 (MQ=255) ttctCGGCATCTATCGACACTTACCGCGAAAGCTATGGGCGAAGAACGAGTGACGTGCCACGTCAGTGTGTTTTcgc < 2:81933/77‑1 (MQ=255) ttctCGGCATCTATCGACACTTACCGCGAAAGCTATGGGCGAAGAACGAGTGACGTGCCACGTCAGTGTGTTTTcgc > 1:81933/1‑77 (MQ=255) cTATCGACACTTACCGCGAAAGCTATGGGCGAAGAACGAGTGACGTGCCACGTCAGTGTGTTTTCGCGGGTACCACCAACCAGGATGAATACCTTAAGGACGCCACCGGTAACCGGCGTTATTGGCCGGTCGCTTGCGTCAAGGTGGAcc < 2:348107/150‑1 (MQ=255) acacTTACCGCGAAAGCTATGGGCGAAGAACGAGTGACGTGCCACGTCAGTGTGTTTTCGCGGGTACCACCAACCAGGATGAATACCTTAAGGACGCCACCGGTAACCGGCGTTATTGGCCGGTCGCTTGCGTCAAGGTGGACCTTGAAg > 2:203368/1‑150 (MQ=255) tGGGCGAAGAACGAGTGACGTGCCACGTCAGTGTGTTTTCGCGGGTACCACCAACCAGGATGAATACCTTAAGGACGCCACCGGTAACCGGCGTTATTGGCCGGTCGCTTGCGTCAAGGTGGACCTTGAAGCATTGCGTCGCGCTCGTGa > 1:16197/1‑150 (MQ=255) gaagaaCGAGTGACGTGCCACGTCAGTGTGTTTTCGCGGGTaccac < 2:147618/46‑1 (MQ=255) gaagaaCGAGTGACGTGCCACGTCAGTGTGTTTTCGCGGGTaccac > 1:147618/1‑46 (MQ=255) gaagaaCGAGTGACGTGCCACGTCAGTGTGTTTTCGCGGGTACCACCAACCAGGATGAATACCTTAAGGACGCCACCGGTAACCGGCGTTATTGGCCGGTCGCTTGCGTCAAGGTGGACCTTGAAGCATTGCGTCGCGCTCGTGACCAGc > 2:345677/1‑150 (MQ=255) actGACGTGGCACGTCAGTGTGTTTTCGCGGGTACCACCAACCAGGATGAATACCTTAAGGACGCCACCGGTAACCGGCGTTATTGGCCGGTCGCTTGCGTCAAGGTGGACCTTGCAGCATTGAGTCGCGCTCGTGACCAGCTGTggggt > 2:208645/3‑148 (MQ=255) actGACGTGGCACGTCAGTGTGTTTTCGCGGGTACCACCAACCAGGATGAATACCTTAAGGACGCCACCGGTAACCGGCGTTATTGGCCGGTCGCTTGCGTCAAGGTGGACCTTGAAGCATTGCGTCGCGCTCGTGACCAGCTGTGGGCt > 2:76063/3‑150 (MQ=255) tGACGTGGCACGTCAGTGTGTTTTCGCGGGTACCACCAACCAGGATGAATACCTTAAGGACGCCACCGGTAACCGGCGTTATTGGCCGGTCGCTTGCGTCAAGGTGGACCTTGAAGCATTGCGTCGCGCTCGTGACCAGCTGTGGGCTGa < 1:208645/150‑1 (MQ=255) tGCCACGTCAGTGTGTTTTCGCGGGTACCACCAACCAGGATGAATACCTTAAGGACGCCACCGGTAACCGGCGTTATTGGCCGGTCGCTTGCGTCAAGGTGGACC‑TGAAGCATTGCGTCGCGCTCGTGACCAGCTGTGGGCTGAGGCCAt < 1:404981/150‑1 (MQ=255) | TTCCAAGCCATCCGAGGTAAGTGGATCGTCGAGCTGGGCGAATTGGACAGCTTCAACAAGGCAGAGAGCACCAAGGCCAAGCAATTCTTCTCGGCATCTATCGACACTTACCGCGAAAGCTATGGGCGAAGAACGAGTGACGTGCCACGTCAGTGTGTTTTCGCGGGTACCACCAACCAGGATGAATACCTTAAGGACGCCACCGGTAACCGGCGTTATTGGCCGGTCGCTTGCGTCAAGGTGGACCTTGAAGCATTGCGTCGCGCTCGTGACCAGCTGTGGGCTGAGGCCAT > NC_002947/3423431‑3423723 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |