| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 3,533,716 | T→G | 16.6% | I117M (ATT→ATG) | atoA → | 3‑oxoacid CoA‑transferase subunit A |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 3,533,716 | 0 | T | G | 16.6% | 47.7 / 2.5 | 24 | I117M (ATT→ATG) | atoA | 3‑oxoacid CoA‑transferase subunit A |
| Reads supporting (aligned to +/- strand): ref base T (11/9); new base G (2/2); total (13/11) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.83e-01 | |||||||||||
GAAGACCGGCAGATTCGCAAGATGATCGCCTCCTACGTGGGCGAAAACGCCGAGTTCGAACGCCAGTTGCTCAGTGGCGAGCTGGAAGTGGAACTGACCCCACAGGGCACCCTCGCCGAAAAAATGCGCGCAGGTGGTGCCGGCATTCCGGCGTTCTACACCGCCACCGGCTATGGCACCCCGGTTGCCGATGGCAAGGAAGTCCGCGAGTTCAAGGGCCGCAAGTACATCCTGGAAGAATCCATCACCGGTGACTTCGCCATCGTCAAGGGCTGGAAGGCC > NC_002947/3533570‑3533851 | gAAGCCCGGCAGATTCGCAAGATGATCGCCTCCTACGTGGGCGAAAACTCCGAGTTCGAACGCCAGTTGCTCAGTGGCGAGCTGGAAGTGGAACTGACCCCACAGGGCACCCTCGCCGAAAAAATGCGCGCAGGTGGTGCCGGCATTCCg < 1:55414/150‑1 (MQ=255) gatgatCGCCTCCTACGTGGGCGAAAACGCCGAGTTCGAACGCCAGTTGCTCAGTGGCGAGCTGGAAGTGGAACTGACCCCACAGGGCACCCTCGCCGAAAAAATGCGCGCAGGTGGTGCCGGCATTCCGGCGTTCTACACCGCCACCgg > 1:167298/1‑150 (MQ=255) tcctACGTGGGCGAAAACGCCGAGTTCGAACGCCAGTTGCTCAGTGGCGAGCTGGAAGTGGAACTGACCCCACAGGGCACCCTCGCCGAAAAAATGCGCGCAGGTGGTGCCGGCATTCCGGCGTTCTACACCGCCACCGGCTATGGCAcc > 2:302097/1‑150 (MQ=255) gCCGAGTTCGAACGCCAGTTGCTCAGTGGCGAGCTGGAAGTGGAACTGACCCCACAGGGCACCCTCGCCGAAAAAATGCGCGCAGGTGGTGCCGGCATTCCGGCGTTCTACACCGCCACCGGCTATGGCACCCCGGTTGCCGATGGCAAg > 1:174387/1‑150 (MQ=255) gAACGCCAGTTGCTCAGTGGCGAGCTGGAAGTGGAACTGACCCCACAGGGCACCCTCGCCGAAAAAATGCGCGCAGGTGGTGCCGGCATTCCGGCGTTCTACACCGCCACCGGCTATGGCACCCCGGTTGCCGATGGCAAGGAAGTCcgc > 2:356659/1‑150 (MQ=255) cAGTTGCTCAGTGGCGAGCTGGAAGTGGAACTGACCCCACAGGGCACCCTCGCCGAAAAAATGCGCGCAGGTGGTGCCGGCATTCCGGCGTTCTACACCGCCACCGGCTATGGCACCCCGGTTGCCGATGGCAAGGAAGTCCGCGAGTTc > 2:125595/1‑150 (MQ=255) gTGGCGAGCTGGAAGTGGAACTGACCCCACAGGGCACCCTCGCCGAAAAAATGCGCGCAGGTGGTGCCGGCATTCCGGCGTTCTACACCGCCACCGGCTATGGCACCCCGGTTGCCGATGGCAAGGAAGTCCGCGAGTTCAAGGGCCGCa > 1:237638/1‑150 (MQ=255) gAGCTGGAAGTGGAACTGACCCCACAGGGCACCCTCGCCGAAAAAATGCGCGCAGGTGGTGCCGGCATTCCGGCGTTCTACACCGCCACCGGCTATGGCACCCCGGTTGCCGATGGCAAGGAAGTCcgc > 1:24715/1‑129 (MQ=255) gAGCTGGAAGTGGAACTGACCCCACAGGGCACCCTCGCCGAAAAAATGCGCGCAGGTGGTGCCGGCATTCCGGCGTTCTACACCGCCACCGGCTATGGCACCCCGGTTGCCGATGGCAAGGAAGTCcgc < 2:24715/129‑1 (MQ=255) ggAAGTGGAACTGACCCCACAGGGCACCCTCGCCGAAAAAATGCGCGCAGGTGGTGCCGGCATTCCGGCGTTCTACACCGCCACCGGCTATGGCACCCCGGTTGCCGATGGCAAGGAAGTCCGCGAGTTCAAGGGCCGCAAGTACATCCt < 1:257143/150‑1 (MQ=255) ggAACTGACCCCACAGGGCACCCTCGCCGAAAAAATGCGCGCAGGTGGTGCCGGCATTCCGGCGTTCTACACCGCCACCGGCTATGGCACCCCGGTTGCCGATGGCAAGGAAGTCCGCGAGTTCAAGGGCCGCAAGTACATCCTGgaaga > 1:224617/1‑150 (MQ=255) cccACAGGGCACCCTCGCCGAAAAAATGCGCGCAGGTGGTGCCGGCATTCCGGCGTTCTACACCGCCACCGGCTATGGCACCCCGGTTGCCGATGGCAAGGAAGTCCGCGAGTTCAAGGGCCGCAAGTACATCCTg < 1:59151/136‑1 (MQ=255) cccACAGGGCACCCTCGCCGAAAAAATGCGCGCAGGTGGTGCCGGCATTCCGGCGTTCTACACCGCCACCGGCTATGGCACCCCGGTTGCCGATGGCAAGGAAGTCCGCGAGTTCAAGGGCCGCAAGTACATCCTg > 2:59151/1‑136 (MQ=255) ccACAGGTCACCCTCGCCGAAAAAATGCGCGCAGGTGGTGCCGGCATTCCGGCGTTCTACACCGCCACCGGCTATGGCACCCCGGTTGCCGATGGCAAGGAAGTCCGCGAGTTCAAGGGCCGCAAGTACATCCTGGAAGAATCCATCAcc > 2:93143/1‑150 (MQ=255) cacaGGGCACCCTCGCCGAAAAAATGCGCGCAGGTGGTGCCGGCATTCCGGCGTTCTACACCGCCACCGGCTATGGCACCCCGGTTGCCGATGGCAAGGAAGTCCGCGAGTTCAAGGGCCGCAAGTACATCCTGGAAGAATCCATCACCg < 2:237638/150‑1 (MQ=255) acaGGGCACCCTCGCCGAAAAAATGCGCGCAGGTGGTGCCGGCATTCCGGCGTTCTACACCGCCACCGGCTATGGCACCCCGGTTGCCGATGGCAAGGAAGTCCGCGAGTTCAAGGGCCGCAAGTACATCCTGGAAGAATCCATCACCgg < 1:302097/150‑1 (MQ=255) tCGCCGAAAAAATGCGCGCAGGTGGTGCCGGCATTCCGGCGTTCTACACCGCCACCGGCTATGGCACCCCGGTTGCCGATGGCAAGGAAGTCCGCGAGTTCAAGGGCCGCAAGTACATCCTGGAAGAATCCATCACCGGTGACTTCGCCa > 1:163499/1‑150 (MQ=255) cGCCGAAAAAATGCGCGCAGGTGGTGCCGGCATTCCGGCGTTCTACACCGCCACCGGCTATGGCACCCCGGTTGCCGATGGCAAGGAAGTCCGCGAGTTCAAGGGCCGCAAGTACATCCTGGAAGAATCCATCACCGGTGACTTCGCCAt < 1:93143/150‑1 (MQ=255) gCCGAAAAAATGCGCGCAGGTGGTGCCGGCATTCCGGCGTTCTACACCGCCACCGGCTATGGCACCCCGGTTGCCGATGGCAAGGAAGTCCGCGAGTTCAAGGGCCGCAAGTACATCCTGGAAGAATCCATCACCGGTGACTTCGCCATc < 1:356659/150‑1 (MQ=255) ggtggtGCCGGCATTCCGGCGTTCTACACCGCCACCGGCTATGGCACCCCGGTTGCCGATGGCAAGGAAGTCCGCGAGTTCAAGGGCCGCAAGTACATCCTGGAAGAATCCATCACCGGTGACTTCGCCATCGTCAAGGGCTGGAAGGcc < 2:163499/150‑1 (MQ=255) gaacgCCGGAATGCCGGCGTCCTACACCGCCACCGGCTATGGCACCCCGGTTGCCGATGGCAAGGAAGTCCGCGAGTTCAAGGGCCGCAAGTACATCCTGgaa > 2:206479/5‑103 (MQ=255) gaacgCCGGAATGCCGGCGTCCTACACCGCCACCGGCTATGGCACCCCGGTTGCCGATGGCAAGGAAGTCCGCGAGTTCAAGGGCCGCAAGTACATCCTGgaa < 1:206479/99‑1 (MQ=255) aacgCCGGAATGCCGGCGTTCTACACCGCCACCGGCTATGGCACCCCGGTTGCCGATGGCAAGGAAGTCCGCGAGTTCAAGGGCCGCAAGTACATCCTGGAAGAATCCATCACCGGt > 1:346248/4‑117 (MQ=255) aacgCCGGAATGCCGGCGTTCTACACCGCCACCGGCTATGGCACCCCGGTTGCCGATGGCAAGGAAGTCCGCGAGTTCAAGGGCCGCAAGTACATCCTGGAAGAATCCATCACCGGt < 2:346248/114‑1 (MQ=255) | GAAGACCGGCAGATTCGCAAGATGATCGCCTCCTACGTGGGCGAAAACGCCGAGTTCGAACGCCAGTTGCTCAGTGGCGAGCTGGAAGTGGAACTGACCCCACAGGGCACCCTCGCCGAAAAAATGCGCGCAGGTGGTGCCGGCATTCCGGCGTTCTACACCGCCACCGGCTATGGCACCCCGGTTGCCGATGGCAAGGAAGTCCGCGAGTTCAAGGGCCGCAAGTACATCCTGGAAGAATCCATCACCGGTGACTTCGCCATCGTCAAGGGCTGGAAGGCC > NC_002947/3533570‑3533851 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |