| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 3,853,162 | T→A | 25.7% | I169N (ATT→AAT) | alkB → | alpha‑ketoglutarate‑ and Fe(II)‑dependent dioxygenase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 3,853,162 | 0 | T | A | 25.7% | 48.9 / 13.6 | 27 | I169N (ATT→AAT) | alkB | alpha‑ketoglutarate‑ and Fe(II)‑dependent dioxygenase |
| Reads supporting (aligned to +/- strand): ref base T (11/9); new base A (4/3); total (15/12) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
ATTACCTGCCGGGCACGCGCCTGAGCCTGCACCAGGACCGCGACGAGCAGGATTTCGGTCAGCCGATCGTGTCGGTGTCGCTGGGCTTACCGGCGGTGTTCCTGTTCGGCGGCCTGCAGCGTGCCGACAAGGCTCAGCGCATTGCGCTGAGCCACGGCGATGTGCTGGTCTGGGGTGGCGTAGACCGCTTGCGCTTCCACGGCGTGCTACCGATCAAACCCGGCGTGCATCCGCGCCTGGGCGAACGGCGGATCAACCTGACC > NC_002947/3853021‑3853283 | aTTACCTGCCGGGCACGCGCCTGAGCCTGCACCAGGACCGCGACGAGCAGGATTTCGGTCAGCCGATCGTGTCGGTGTCGCTGGGCTTACCGGCGGTGTTCCTGTTCGGCGGCCTGCAGCGTGCCGACAAGGCTCAGCGCATTGCGCTGa > 1:54232/1‑150 (MQ=255) gCCTGCACCAGGACCGCGACGAGCAGGATTTCGGTCAGCCGATCGTGTCGGTGTCGCTGGGCTTACCGGCGGTGTTCCTGTTCGGCGGCCTGCAGCGTGCCGACAAGGCTCAGCGCATTGCGCTGAGCCACGGCGATGTGCTGGTCTggg < 2:43725/150‑1 (MQ=255) gCCTGCACCAGGACCGCGACGAGCAGGATTTCGGTCAGCCGATCGTGTCGGTGTCGCTGGGCTTACCGGCGGTGTTCCTGTTCGGCGGCCTGCAGCGTGCCGACAAGGCTCAGCGCATTGCGCTGAGCCACGGCGATGTGCTGGTCTggg > 2:394661/1‑150 (MQ=255) aCCAGGACCGCGACGAGCAGGATTTCGGTCAGCCGATCGTGTCGGTGTCGCTGGGCTTACCGGCGGTGTTCCTGTTCGGCGGCCTGCAGCGTGCCGACAAGGCTCAGCGCATTGCGCTGAGCCACGGCGATGTGCTGGTCTGGGGTGGCg > 2:265585/1‑150 (MQ=255) tttCGGTCAGCCGATCGTGTCGGTGTCGCTGGGCTTACCGGCGGTGTTCCTGTTCGGCGGCCTGCAGCGTGCCGACAAGGCTCAGCGCAATACACTGAGCCACGGCGATGTGCAGGTCTGGGGTGGCGTAGACCGCTTGCGCTTCCACgg > 1:260562/1‑150 (MQ=255) gTCAGCCGATCGTGTCGGTGTCGCTGGGCTTACCGGCGGTGTTCCTGTTCGGCGGCCTGCAGCGTGCCGACAAGGCTCAGCGCATTGCGCTGAGCCACGGCGATGTGCTGGTCTGGGGTGGCGTAGACCGCTTGCGCTTCCACGGCGTGc < 1:400114/150‑1 (MQ=255) gTCAGCCGATCGTGTCGGTGTCGCTGGGCTTACCGGCGGTGTTCCTGTTCGGCGGCCTGCAGCGTGCCGACAAGGCTCAGCGCATTGCGCTGAGCCACGGCGATGTGCTGGTCTGGGGTGGCGTAGACCGCTTGCGCTTCCACGGCGTGc > 1:401916/1‑150 (MQ=255) ccGATCGTGTCGGTGTCGCTGGGCTTACCGGCGGTGTTCCTGTTCGGCGGCCTGCAGCGTGCCGACAAGGCTCAGCGCATTGCGCTGAGCCACGGCGATGTGCTGGTCTGGGGTGGCGTAGACCGCTTGCGCTTCCACGGCGTGCTACCg > 2:178484/1‑150 (MQ=255) gtgtcgCTGGGCTTACCGGCGGTGTTCCTGTTCGGCGGCCTGCAGCGTGCCGACAAGGCTCAGCGCATTGCGCTGAGCCACGGCGATGTGCTGGTCTGGGGTGGCGTAGACCGCTTGCGCTTCCACGGCGTGCTACCGATCAAACCCGGc < 2:401916/150‑1 (MQ=255) ggTGTTCCTGTTCGGCGGCCTGCAGCGTGCCGACAAGGCTCAGCGCATTGCGCTGAGCCACGGCGATGTGCTGGTCTGGGGTGGCGTAGACCGCTTGCGCTTCCACGGCGt < 2:405720/111‑1 (MQ=255) ggTGTTCCTGTTCGGCGGCCTGCAGCGTGCCGACAAGGCTCAGCGCATTGCGCTGAGCCACGGCGATGTGCTGGTCTGGGGTGGCGTAGACCGCTTGCGCTTCCACGGCGt > 1:405720/1‑111 (MQ=255) ttCCTGTTCGGCGGCCTGCAGCGTGCCGACAAGGCTCAGCGCATTGCGCTGAGCCACGGCGATGTGCTGGTCTGGGGTGGCGTAGACCGCTTGCGCTTCCACGGCGTGCTACCGATCAAACCCGGCGTGCATCCGCGCCTGGGCGAAcgg > 1:296871/1‑150 (MQ=255) ttCCTGTTCGGCGGCCTGCAGCGTGCCGACAAGGCTCAGCGCATTGCGCTGAGCCACGGCGATGTGCTGGTCTGGGGTGGCGTAGACCGCTTGCGCTTCCACGGCGTGCTACCGATCAAACCCGGCGTGCATCCGCGCCTGGGCGAAcgg > 1:357835/1‑150 (MQ=255) tGTTCGGCGGCCTGCAGCGTGCCGACAAGGCTCAGCGCATTGCGCTGAGCCACGGCGATGTGCTGGTCTGGGGTGGCGTAGACCGCTTGCGCTTCCACGGCGTGCTACCGATCAAACCCGGCGTGCATCCGCGCCTGGGCGAACGGCGGa < 2:357835/150‑1 (MQ=255) gTTCGGCGGCCTGCAGCATGCCGACAAGGCTCAGCGCATTGCGCTGAGCCACGGCGATGTGCTGGTCTGGGGTGGCGTAGACCGCTTGCGCTTCCACGGCGTGCTACCGATCAAACCCGGCGTGCATc < 1:166854/128‑1 (MQ=255) gTTCGGCGGCCTGCAGCATGCCGACAAGGCTCAGCGCATTGCGCTGAGCCACGGCGATGTGCTGGTCTGGGGTGGCGTAGACCGCTTGCGCTTCCACGGCGTGCTACCGATCAAACCCGGCGTGCATc > 2:166854/1‑128 (MQ=255) ggcCTGCAGCGTGCCGACAAGGCTCAGCGCATTGCGCTGAGCCACGGCGATGTGCTGGTCTGGGGTGGCGTAGACCGCTTGCGCTTCCACGGCGTGCTACCGATCAAACCCGGCGTGCATCCGCGCCTGGGCGAACGGCGGATCAACCTg > 1:244837/1‑150 (MQ=255) ccTGCAGCGTGCCGACAAGGCTCAGCGCATTGCGCTGAGCCACGGCGATGTGCTGGTCTGGGGTGGCGTAGACCGCTTGCGCTTCCACGGCGTGCTACCGATCAAACCCGGCGTGCATCCGCGCCTGGGCGAACGGCGGATCAACCTGAc < 1:84871/150‑1 (MQ=255) cTGCAGCGTGCCGACAAGGCTCAGCGCATTGCGCTGAGCCACGGCGATGTGCTGGTCTGGGGTGGCGTAGACCGCTTGCGCTTCCACGGCGTGCTACCGATCAAACCCGGCGTGCATCCGCGCCTGGGCGAACGGCGGATCAACCTGAcc < 2:296871/150‑1 (MQ=255) ccGACAAGGCTCAGCGCATTGCGCTGAGCCACGGCGATGTGCTGGTCTGGGGTGGCGTAGACCGCTTGCGCTTCCACGGCGTGCTACCGATCAAACCCGGc > 2:265472/1‑101 (MQ=255) ccGACAAGGCTCAGCGCATTGCGCTGAGCCACGGCGATGTGCTGGTCTGGGGTGGCGTAGACCGCTTGCGCTTCCACGGCGTGCTACCGATCAAACCCGGc < 1:265472/101‑1 (MQ=255) tCAGCGCAATGCGCTGAGCCACGGCGATGTGCTGGTCTGGGGTGGCGTAGACCGCTTGCGCTTCCACGGCGTGCTAc > 2:153765/1‑77 (MQ=255) tCAGCGCAATGCGCTGAGCCACGGCGATGTGCTGGTCTGGGGTGGCGTAGACCGCTTGCGCTTCCACGGCGTGCTAc < 1:153765/77‑1 (MQ=255) tCAGCGCAATGCGCTGAGCCACGGCGATGTGCTGGTCTGGGGTGGCGTAGACCGCTTGCGCTTCCACGGCGTGCTACCGATCAAACCCGGCGTGCATCCGCGCCTGGGCGAAcgg < 1:416785/115‑1 (MQ=255) tCAGCGCAATGCGCTGAGCCACGGCGATGTGCTGGTCTGGGGTGGCGTAGACCGCTTGCGCTTCCACGGCGTGCTACCGATCAAACCCGGCGTGCATCCGCGCCTGGGCGAAcgg < 1:416708/115‑1 (MQ=255) tCAGCGCAATGCGCTGAGCCACGGCGATGTGCTGGTCTGGGGTGGCGTAGACCGCTTGCGCTTCCACGGCGTGCTACCGATCAAACCCGGCGTGCATCCGCGCCTGGGCGAAcgg > 2:416708/1‑115 (MQ=255) tCAGCGCAATGCGCTGAGCCACGGCGATGTGCTGGTCTGGGGTGGCGTAGACCGCTTGCGCTTCCACGGCGTGCTACCGATCAAACCCGGCGTGCATCCGCGCCTGGGCGAAcgg > 2:416785/1‑115 (MQ=255) | ATTACCTGCCGGGCACGCGCCTGAGCCTGCACCAGGACCGCGACGAGCAGGATTTCGGTCAGCCGATCGTGTCGGTGTCGCTGGGCTTACCGGCGGTGTTCCTGTTCGGCGGCCTGCAGCGTGCCGACAAGGCTCAGCGCATTGCGCTGAGCCACGGCGATGTGCTGGTCTGGGGTGGCGTAGACCGCTTGCGCTTCCACGGCGTGCTACCGATCAAACCCGGCGTGCATCCGCGCCTGGGCGAACGGCGGATCAACCTGACC > NC_002947/3853021‑3853283 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |