| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 3,990,126 | C→T | 10.8% | Q123* (CAG→TAG) | PP_3516 → | AraC family transcriptional regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 3,990,126 | 0 | C | T | 10.8% | 92.9 / 3.1 | 37 | Q123* (CAG→TAG) | PP_3516 | AraC family transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base C (17/16); new base T (2/2); total (19/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.74e-01 | |||||||||||
CGGCCAGGACCATGAAGCGTTCTACCTGGTGACCGTGCCGCATGGCAGCGAGGTGCACTTTGAACAGGACGGCCACCAGATCAGTTGCAGCCCGGGCGGTTTCATCGTCGAGCGCGGCGATGCACCCTACCGCTTCCACTACGGCACGCAGAACGACTTGTGGGTGCTCAAGCTGCCCGAGCGTGCGCTCAAAGCCAACCTGCTGGGGCACAAGCGCTATACCCGGCATTGCTTCGACGCCCGTCAGGGCCTGGGGCGCATCTTCGTCGAGCAACTCGACCTGTGTGCCCGCC > NC_002947/3989978‑3990270 | cGGCCAGGACCATGAAGCGTTCTACCTGGTGACCGTGCCGCATGGCAGCGAGGTGCACTTTGAACAGGACGGCCACCAGATCAGTTGCAGCCCGGGCGGTTTCATCGTCGAGCGCGGCGATGCACCCTACCGCTTCCACTACGGCACGCa < 1:453574/150‑1 (MQ=255) gAAGCGTTCTACCTGGTGACCGTGCCGCATGGCAGCGAGGTGCACTTTGAACAGGACGGCCACCAGATCAGTTGCAGCCCGGGCGGTTTCATCGTCGAGCGCGGCGATGCACCCTACCGCTTCCACTACGGCACGCAGAACGACTTGTgg < 2:116851/150‑1 (MQ=255) gTTCTACCTGGTGACCGTGCCGCATGGCAGCGAGGTGCACTTTGAACAGGACGGCCACCAGATCAGTTGCAGCCCGGGCGGTTTCATCGTCGAGCGCGGCGATGCACCCTACCGCTTCCACTACGGCACGCAGAACGACTTGTGGGTGCt > 2:316182/1‑150 (MQ=255) ccTGGTGACCGTGCCGCATGGCAGCGAGGTGCACTTTGAACAGGACGGCCACCAGATCAGTTGCAGCCCGGGCGGTTTCATCGTCGAGCGCGGCGATGCACCCTACCGCTTCCACTACGGCACGCAGAACGACTTGTGGGTGCTCAAGCt < 1:251330/150‑1 (MQ=255) cGTGCCGCATGGCAGCGAGGTGCACTTTGAACAGGACGGCCACCAGATCAGTTGCAGCCCGGGCGGTTTCATCGTCGAGCGCGGCGATGCACCCTACCGCTTCCACTACGGCACGCAGAACGACTTGTGGGTGCTCAAGCTGCCCGAGCg > 1:462486/1‑150 (MQ=255) aTGGCAGCGAGGTGCACTTTGAACAGGACGGCCACCAGATCAGTTGCAGCCCGGGCGGTTTCATCGTCGAGCGCGGCGATGCACCCTACCGCTTCCACTACGGCACGCAGAACGACTTGTGGGTGCTCAAGCTGCCCGAGCGTGCGCTCa > 2:21789/1‑150 (MQ=255) gCAGCGAGGTGCACTTTGAACAGGACGGCCACCAGATCAGTTGCAGCCCGGGCGGTTTCATCGTCGAGCGCGGCGATGCACCCTACCGCTTCCACTACGGCACGCAGAACGACTTGTGGGTGCTCAAGCTGCCCGAGCGTGCGCTCAAAg > 1:91231/1‑150 (MQ=255) cTTTGAACAGGACGGCCACCAGATCAGTTGCAGCCCGGGCGGTTTCATCGTCGAGCGCGGCGATGCACCCTACCGCTTCCACTACGGCACGCAGAACGACTTGTGGGTGCTCAAGCTGCCCGAGCGTGCGCTCAAAGCCAACCTGCTggg < 2:462486/150‑1 (MQ=255) cTTTGAACAGGACGGCCACCAGATCAGTTGCAGCCCGCGTTGTTTCATCGTAGAGCGCGGCGATGCCGGTTCCTTCTTCCACTACGGCACGCAGAACGACTTGTGGGTGCTCAAGCTGCCCGAGCGTGCGCTCAAAGCCAACCTGCTggg < 1:391529/150‑1 (MQ=255) ggACGGCCACCAGATCAGTTGCAGCCCGGGCGGTTTCATCGTCGAGCGCGGCGATGCACCCTACCGCTTCCACTACGGCACGCAGAACGACtt < 2:364177/93‑1 (MQ=255) ggACGGCCACCAGATCAGTTGCAGCCCGGGCGGTTTCATCGTCGAGCGCGGCGATGCACCCTACCGCTTCCACTACGGCACGCAGAACGACtt > 1:364177/1‑93 (MQ=255) ccaccaGATCAGTTGCAGCCCGGGCGGTTTCATCGTCGAGCGCGGCGATGCACCCTACCGCTTCCACTACGGCACGCAGAACGACTTGTGGGTGCTCAAGCTGCCCGAGCGTGCGCTCAAAGCCAACCTGCTGGGGCACAAGCGCTATAc > 2:384851/1‑150 (MQ=255) caccaGATCAGTTGCAGCCCGGGCGGTTTCATCGTCGAGCGCGGCGATGCACCCTACCGCTTCCACTACGGCACGCAGAACGACTTGTGGGTGCTCAAGCTGCCCGAGCGTGCGCTCAAAGCCACCCTGCTGGGGCACAAGCGCTATAcc > 1:87781/1‑150 (MQ=255) aGATCAGTTGCAGCCCGGGCGGTTTCATCGTCGAGCGCGGCGATGCACCCTACCGCTTCCACTACGGCACGCAGAACGACTTGTGGGTGCTCAAGCTGCCCGAGCGTGCGCTCAAAGCCAACCTGCTGGGGCACAAGCGCTATACCCGGc < 2:392547/150‑1 (MQ=255) gTTGCAGCCCGGGCGGTTTCATCGTCGAGCGCGGCGATGCACCCTACCGCTTCCACTACGGCACGCAGAACGACTTGTgg < 1:206812/80‑1 (MQ=255) gTTGCAGCCCGGGCGGTTTCATCGTCGAGCGCGGCGATGCACCCTACCGCTTCCACTACGGCACGCAGAACGACTTGTgg > 2:206812/1‑80 (MQ=255) ggTTTCATCGTCGAGCGCGGCGATGCACCCTACCGCTTCCACTACGGCACGCAGAACGACTTGTGGGTGCTCAAGCTGCCCGAGCGTGCGCTCAAAGCCAACCTGCTGGGGCACAAGCGCTATACCCGGCATTGCTTCGACGCCCGTCAg < 2:91231/150‑1 (MQ=255) aTCGTCGAGCGCGGCGATGCACCCTACCGCTTCCACTACGGCACGCAGAACGACTTGTGGGTGCTCAAGCTGCCCGAGCGTGCGCTCAAAGCCAACCTGCTGGGGCACAAGCGCTATACCCGGCATTGCTTCGACGCCCGTCAGGGCCTg > 1:219805/1‑150 (MQ=255) aGCGCGGCGATGCACCCTACCGCTTCCACTACGGCACGCAGAACGACTTGTGGGTGCTCAAGCTGCCCGAGCGTGCGCTCAAAGCCAACCTGCTGGGGCCCAAGCGCTATACCCGGCCTTGCTTCGACGCCCAACAAGCCCCGGGGCGCa > 2:411096/1‑150 (MQ=255) cgcgGCGATGCACCCTACCGCTTCCACTACGGCACGCAGAACGACTTGTGGGTGCTCAAGCTGCCCGAGCGTGCGCTCAAAGCCAACCTGCTGGGGCACAAGCGCTATACCCGGCATTGCTTCGACGCCCGTCAGGGCCTGGGGCGCATc < 1:316182/150‑1 (MQ=255) gcggcgATGCACCCTACCGCTTCCACTACGGCACGCAGAACGACTTGTGGGTGCTCAAGCTGCCCGAGCGTGCGCTCAAAGCCAACCTGCTGGGGCACAAGCGCTATACCCGGCATTGCTTCGACGCCCGTCAGGGCCTGGGgc < 1:19009/144‑1 (MQ=255) gcggcgATGCACCCTACCGCTTCCACTACGGCACGCAGAACGACTTGTGGGTGCTCAAGCTGCCCGAGCGTGCGCTCAAAGCCAACCTGCTGGGGCACAAGCGCTATACCCGGCATTGCTTCGACGCCCGTCAGGGCCTGGGgc > 2:19009/1‑144 (MQ=255) tGCACCCTACCGCTTCCACTACGGCACGCAGAACGACTTGTGGGTGCTCAAGCTGCCCGAGCGTGCGCTCAAAGCCAACCTGCTGGGGCACAAGCGCTATACCCGGCATTGCTTCGACGCCCGTCAGGGCCTGGGGCGCATCTTCGTCGa < 1:186379/150‑1 (MQ=255) gCACCCTACCGCTTCCACTACGGCACGCAGAACGACTTGTGGGTGCTCAAGCTGCCCGAGCGTGCGCTCAAAGCCAACCTGCTGGGGCACAAGCGCTATACCCGGCATTGCTTCGACGCCCGTCAGGGCCTGGGGCGCATCTTCGTCGAg < 2:132046/150‑1 (MQ=255) aCCCTACCGCTTCCACTACGGCACGTAGAACGACTTGTGGGTGCTCAAGCTGCCCGAGCGTGCGCTCAAAGCCAACCTGCTGGGGCACAAGCGCTATACCCGGCATTGCTTCGACGCCCGTCAGGGCCTGGGGCGCATCTTCGTCGAGCa > 2:214355/1‑150 (MQ=255) ccTACCGCTTCCACTACGGCACGCAGAACGACTTGTGGGTGCTCAAGCTGCCCGAGCGTGCGCTCAAAGCCAACCTGCTGGGGCACAAGCGCTATACCCGGCATTGCTTCGACGCCCGTCAGGGCCTGGGGCGCATCTTCGTCGAGCAAc > 2:101061/1‑150 (MQ=255) cTACCGCTTCCACTACGGCACGCAGAACGACTTGTGGGTGCTCAAGCTGCCCGAGCGTGCGCTCAAAGCCAACCTGCTGGGGCACAAGCGCTATACCCGGCATTGCTTCGACGCCCGTCAGGGCCTGGGGCGCATCTTCGTCGAGCAACt > 2:10402/1‑150 (MQ=255) tACCGCTTCCACTACGGCACGCAGAACGACTTGTGGGTGCTCAAGCTGCCCGAGCGTGCGCTCAAAGCCAACCTGCTGGGGCACAAGCGCTATACCCGGCATTGCTTCGACGCCCGTCAGGGCCTGGGGCGCATCTTCGTCGAGCAACTc < 1:10402/150‑1 (MQ=255) tACCGCTTCCACTACGGCACGCAGAACGACTGGTGGGTGCTCACGCTCACCGAGCGGGCGCTCAAAGCCAACCTGCTGGGGCACAAGCGCTATACCAGGCATTGCTTCGACGCCCGTCAGGGCCTGGGGCGCATCTTCGTCGAGCAACTc > 1:395174/1‑150 (MQ=255) gCTTCCACTACGGCACGTAGAACGACTTGTGGGTGCTCAAGCTGCCCGAGCGTGCGCTCAAAGCCAACCTGCTGGGGCACAAGCGCTATACCCGGCATTGCTTCGACGCCCGTCAGGGCCTGGGGCGCATCTTCGTCGAGCAACTCGAc > 2:43737/1‑149 (MQ=255) gCTTCCACTACGGCACGTAGAACGACTTGTGGGTGCTCAAGCTGCCCGAGCGTGCGCTCAAAGCCAACCTGCTGGGGCACAAGCGCTATACCCGGCATTGCTTCGACGCCCGTCAGGGCCTGGGGCGCATCTTCGTCGAGCAACTCGAc < 1:43737/149‑1 (MQ=255) cTTCCACTACGGCACGCAGAACGACTTGTGGGTGCTCAAGCTGCCCGAGCGTGCGCTCAAAGCCAACCTGCTGGGGCACAAGCGCTATACCCGGCATTGCTTCGACGCCCGTCAGGGCCTGGGGCGCa > 2:178410/1‑128 (MQ=255) cTTCCACTACGGCACGCAGAACGACTTGTGGGTGCTCAAGCTGCCCGAGCGTGCGCTCAAAGCCAACCTGCTGGGGCACAAGCGCTATACCCGGCATTGCTTCGACGCCCGTCAGGGCCTGGGGCGCa < 1:178410/128‑1 (MQ=255) tCCACTACGGCACGTAGAACGACTTGTGGGTGCTCAAGCTGCCCGAGCGTGCGCTCAAAGCCAACCTGCTGGGGCACAAGCGCTATACCCGGCATTGCTTCGACGCCCGTCAGGGCCTGGGGCGCATCTTCGTCGAGCAACTCGACCtgt < 1:214355/150‑1 (MQ=255) gCACGCAGAACGACTTGTGGGTGCTCAAGCTGCCCGAGCGTGCGCTCAAAGCCGACCTGCTGGGGCACAAGCGCTATACCCGGCATTGCTTCGACGCCCGTCAGGGCCTGGGGCGCATCTTCGTCGAGCAACTCGACCTGTGTGCCCGcc > 1:375752/1‑150 (MQ=255) gCAGAACGACTTGTGGGTGCTCAAGCTGCCCGAGCGTGCGCTCAAAGCCAACCTGCTGGGGCACAAgcgc < 1:368426/70‑1 (MQ=255) gCAGAACGACTTGTGGGTGCTCAAGCTGCCCGAGCGTGCGCTCAAAGCCAACCTGCTGGGGCACAAgcgc > 2:368426/1‑70 (MQ=255) | CGGCCAGGACCATGAAGCGTTCTACCTGGTGACCGTGCCGCATGGCAGCGAGGTGCACTTTGAACAGGACGGCCACCAGATCAGTTGCAGCCCGGGCGGTTTCATCGTCGAGCGCGGCGATGCACCCTACCGCTTCCACTACGGCACGCAGAACGACTTGTGGGTGCTCAAGCTGCCCGAGCGTGCGCTCAAAGCCAACCTGCTGGGGCACAAGCGCTATACCCGGCATTGCTTCGACGCCCGTCAGGGCCTGGGGCGCATCTTCGTCGAGCAACTCGACCTGTGTGCCCGCC > NC_002947/3989978‑3990270 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |