| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 4,002,532 | G→A | 17.4% | V224V (GTG→GTA) | allE → | S‑ureidoglycine aminohydrolase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 4,002,532 | 0 | G | A | 17.4% | 45.1 / 4.6 | 23 | V224V (GTG→GTA) | allE | S‑ureidoglycine aminohydrolase |
| Reads supporting (aligned to +/- strand): ref base G (8/11); new base A (2/2); total (10/13) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.08e-01 | |||||||||||
ACCCGTTTTGTCGACATGGCCGACATGCGCCACGACATGCACGTGAACATCGTCACCTTCCAGCCGGGCGGTGTGATTCCGTTCGCAGAAACCCACGTGATGGAACACGGCCTGTACGTGCTGGAAGGCAAGGCGGTGTACCGCCTGAACCAGGACTGGGTCGAAGTGGAAGCCGGCGACTTCATGTGGCTGCGCGCCTTCTGCCCGCAAGCCTGCTACGCCGGCGGCCCAGGCAACTTTAGCTACCTGCTGTACAAGGACGTGAACCGTCACGTACACCTGAC > NC_002947/4002395‑4002678 | accCGTTTTGTCGACATGGCCGACATGCGCCACGACATGCACGTGAACATCGTCACCTTCCAGCCGGGCGGTGTGATTCCGTTCGCAGAAACCCACGTGATGGAACACGGCCTGTACGTGCTGGAAGGCAAGGCGGTGTACCGCCTGAAc > 1:300382/1‑150 (MQ=255) cccGTTTTGTCGACATGGCCGACATGCGCCACGACATGCACGTGAACATCGTCACCTTCCAGCCGGGCGGTGTGATTCCGTTCGCAGAAACCCACGTGATGGAACACGGCCTGTACGTGCTGGAAGGCAAGGCGGTACACCGCCtt < 2:448601/146‑2 (MQ=255) cccGTTTTGTCGACATGGCCGACATGCGCCACGACATGCACGTGAACATCGTCACCTTCCAGCCGGGCGGTGTGATTCCGTTCGCAGAAACCCACGTGATGGAACACGGCCTGTACGTGCTGGAAGGCAAGGCGGTACACCGCCtt > 1:448601/1‑145 (MQ=255) cGTTTTGTCGACATGGCCGACATGCGCCACGACATGCACGTGAACATCGTCACCTTCCAGCCGGGCGGTGTGATTCCGTTCGCAGAAACCCACGTGATGGAACACGGCCTGTACGTGCTGGAAGGCAAGGCGGTGTACCGCCTGAACCAg < 2:509300/150‑1 (MQ=255) gACATGCGCCACGACATGCACGTGAACATCGTCACCTTCCAGCCGGGCGGTGTGATTCCGTTCGCAGAAACCCACGTGATGGAACACGGTCTGTACGTGCTGGAAGGCAAGGCGGTGTACCGCCTGAACCAGGACTGGGTCGAAGTGGaa < 1:359667/150‑1 (MQ=255) gcCACGACATGCACGTGAACATCGTCACCTTCCAGCCGGGCGGTGTGATTCCGTTCGCAGAAACCCACGTGATGGAACACGGCCTGTACGTGCTGGAAGGCAAGGCGGTGTACCGCCTGAACCAGGACTGGGTCGAAGTGGAAGCCGGCg > 1:377179/1‑150 (MQ=255) aTGCACGTGAACATCGTCACCTTCCATCCTGGCGGTGTGATTCCGTTCGCAGAAACCCACGTGATGGAACACGGCCTGTACGTGCTGGAAGGCAAGGCGGTGTACCGCCTGAACCAGGACTGGGTCGAAGTGGAAGCCGGCGACTTCAtg > 1:68765/1‑150 (MQ=255) aCGTGAACATCGTCACCTTCCAGCCGGGCGGTGTGATTCCGTTCGCAGAAACCCACGTGATGGAACACGGCCTGTACGTGCTGGAAGGCAAGGCGGTACACCGCCtt > 1:425267/1‑106 (MQ=255) aCGTGAACATCGTCACCTTCCAGCCGGGCGGTGTGATTCCGTTCGCAGAAACCCACGTGATGGAACACGGCCTGTACGTGCTGGAAGGCAAGGCGGTACACCGCCtt < 2:425267/107‑2 (MQ=255) cAGCCGGGCGGTGTGATTCCGTTCGCAGAAACCCACGTGATGGAACACGGCCTGTACGTGCTGGAAGGCAAGGCGGTGTACCGCCTGAACCAGGACTGGGTCGAAGTGGAAGCCGGCGACTTCATGTGGCTGCGCGCCTTCTGCCCGCaa < 2:130050/150‑1 (MQ=255) gCGGTGTGATTCCGTTCGCAGAAACCCACGTGATGGAACACGGCCTGTACGTGCTGGAAGGCAAGGCGGTGTACCGCCTGAACCAGGACTGGGTCGAAGTGGAAGCCGGCGACTTCATGTGGCTGCGCGCCTTCTGCCCGCAAGCCTGCt < 1:226359/150‑1 (MQ=255) ttCCGTTCGCAGAAACCCACGTGATGGAACACGGCCTGTACGTGCTGGAAGGCAAGGCGGTGTACCGCCTGAACCAGGACTGGGTCGAAGTGGAAGCCGGCGACTTCATGTGGCTGCGCGCCTTCTGCCCGCAAGCCTGCTACGCcggcg > 2:34037/1‑150 (MQ=255) cAGAAACCCACGTGATGGAACACGGCCTGTACGTGCTGGAAGGCAAGGCGGTGTACCGCCTGAACCAGGACTGGGTCGAAGTGGAAGCCGGCGACTTCATGTGGCTGCGCGCCTTCTGCCCGCAAGCATGATACGCCGGCGGCCCAGGCa > 2:93219/1‑150 (MQ=255) cccACGTGATGGAACACGGCCTGTACGTGCTGGAAGGCAAGGCGGTGTACCGCCTGAACCAGGACTGGGTCGAAGTGGAAGCCGGCGACTTCATGTGGCTGCGCGCCTTCTGCCCGCAAGCCTGCTACGCCGGCGGCCCAGGCAACTTTa < 2:377179/150‑1 (MQ=255) aCGTGATGGAACACGGCCTGTACGTGCTGGAAGGCAAGGCGGTGTACCGCCTGAACCAGGACTGGGTCGAAGTGGAAGCCGGCGACTTCATGTGGCTGCGCGCCTTCTGCCCGCAAGCCTGCTACGCcggcg > 2:17946/1‑132 (MQ=255) aCGTGATGGAACACGGCCTGTACGTGCTGGAAGGCAAGGCGGTGTACCGCCTGAACCAGGACTGGGTCGAAGTGGAAGCCGGCGACTTCATGTGGCTGCGCGCCTTCTGCCCGCAAGCCTGCTACGCcggcg < 1:17946/132‑1 (MQ=255) cGGCCTGTACGTGCTGGAAGGCAAGGCGGTGTACCGCCTGAACCAGGACTGGGTCGAAGTGGAAGCCGGCGACTTCATGTGGCTGCGCGCCTTCTGCCCGCAGGCCTGCTACGCCGGCGGCCCAGGCAACTTTAGCTACCTGCTGTACac < 1:93219/150‑2 (MQ=255) ccTGTACGTGCTGGAAGGCAAGGCGGTGTACCGCCTGAACCAGGACTGGGTCGAAGTGGAAGCCGGCGACTTCATGTGGCTGCGCGCCTTCTGCCCGCAAGCCTGCTACGCCGGCGGCCCAGGCAACTTTAGCTACCTGCTGTACAAGGa < 1:34037/150‑1 (MQ=255) cTGTACGTGCTGGAAGGCAAGGCGGTGTACCGCCTGAACCAGGACTGGGTCGAAGTGGAAGCCGGCGACTTCATGTGGCTGCGCGCCTTCTGCCCGCAAGCCTGCTACGCcgg > 1:64473/1‑113 (MQ=255) cTGTACGTGCTGGAAGGCAAGGCGGTGTACCGCCTGAACCAGGACTGGGTCGAAGTGGAAGCCGGCGACTTCATGTGGCTGCGCGCCTTCTGCCCGCAAGCCTGCTACGCcgg < 2:64473/113‑1 (MQ=255) gTGCTGGAAGGCAAGGCGGTGTACCGCCTGAACCAGGACTGGGTCGAAGTGGAAGCCGGCGACTTCATGTGGCTGCGCGCCTTCTGCCCGCAAGCCTGCTACGCCGGCGGCCCAGGCAACTTTAGCTACCTGCTGTACAAGGACGTGAAc > 2:341625/1‑150 (MQ=255) gAAGGCAAGGCGGTGTACCGCCTGAACCAGGACTGGGTCGAAGTGGAAGCCGGCGACTTCATGTGGCTGCGCGCCTTCTGCCCGCAAGCCTGCTACGCCGGCGGCCCAGGCAACTTTAGCTACCTGCTGTACAAGGACGTGAACCGTCAc < 1:341625/150‑1 (MQ=255) ggTGTACCGCCTGAACCAGGACTGGGTCGAAGTGGAAGCCGGCGACTTCATGCGGCTGCGCGCCTTCTGCCCGCAAGCCTGCTACGCCGGCGGCCCAGGCAACTTTAGCTACCTGCTGTACAAGGACGTGAACCGTCACGTACACCTGAc < 2:68765/150‑1 (MQ=255) | ACCCGTTTTGTCGACATGGCCGACATGCGCCACGACATGCACGTGAACATCGTCACCTTCCAGCCGGGCGGTGTGATTCCGTTCGCAGAAACCCACGTGATGGAACACGGCCTGTACGTGCTGGAAGGCAAGGCGGTGTACCGCCTGAACCAGGACTGGGTCGAAGTGGAAGCCGGCGACTTCATGTGGCTGCGCGCCTTCTGCCCGCAAGCCTGCTACGCCGGCGGCCCAGGCAACTTTAGCTACCTGCTGTACAAGGACGTGAACCGTCACGTACACCTGAC > NC_002947/4002395‑4002678 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |