| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 4,049,954 | C→G | 25.0% | intergenic (+43/‑58) | quiA → / → oprB‑III | quinate dehydrogenase/carbohydrate‑selective porin |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 4,049,954 | 0 | C | G | 25.0% | 32.4 / 7.5 | 16 | intergenic (+43/‑58) | quiA/oprB‑III | quinate dehydrogenase/carbohydrate‑selective porin |
| Reads supporting (aligned to +/- strand): ref base C (8/4); new base G (2/2); total (10/6) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.04e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.13e-01 | |||||||||||
CCTACGTCTCGCCGAAGACCGGCAAGCAGTACGTGGTGATCACTGCTGGCGGCGCCCGCCAGTCGACTGACCGTGGCGACTATGTGATTTCTTACGCTCTGCCGTAAAACCGCGTCGCCTGCTTCGCGGGCAAGCCCGCTCCTACAAGGCCCCTGTAGGAGCGGGCTTGCCCGCGAATGCCCCTCAAAGACAACCCGAGATTCTGTAATGCCATCCGCAATTCGCATCACCCCCACCCTGCTGCTGGCCCTGGCCAGCACCACTGCCCTTGCCAGCGACGATCTCATGA > NC_002947/4049805‑4050093 | ccTACGTCTCGCCGAAGACCGGCAAGCAGTACGTGGTGATCACTGCTGGCGGCGCCCGCCAGTCGACTGACCGTGGCGACTATGTGATTTCTTACGCTCTGCCGTAAAACCGCGTCGCCTGCTTCGCGGGCAAGCCCGCTCCTACAAGGc > 1:452876/1‑150 (MQ=255) cGGCAAGCAGTACGTGGTGATCACTGCTGGCGGCGCCCGCCAGTCGACTGACCGTGGCGACTATGTGATTTCTTACGCTCTGCCGTAAAACCGCGTCGCCTGCTTCGCGGGCAAGCCCGCTCCTACAAGGCCCCTGTAGGAGCGGGCTTg > 2:326123/1‑150 (MQ=255) gCAGTACGTGGTGATCACTGCTGGCGGCGCCCGCCAGTCGACTGACCGTGGCGACTATGTGATTTCTTACGCTCTGCCGTAAAACCGCGTCGCCTGCTTCGCGGGCAAGCCCGCTCCTACAAGGCCCCTGTAGGAGCGGGCTTGCCcgcg > 2:323235/1‑150 (MQ=255) tgATCACTGCTGGCGGCGCTCGCCAGTCGACTGACCGTGGCGACTATGTGATTTCTTACGCTCTGCCGTAAAACCGCGTCGCCTGCTTCGCGGGCAAGCCCGCTCCTACAAGGCCCCTGTAGGAGCGGGCTTGCCCGCGAATGCCCCTCa > 1:311625/1‑150 (MQ=255) aTCACTGCTGGCGGCGCTCGCCAGTCGACTGACCGTGGCGACTATGTGATTTCTTACGCTCTGCCGTAAAACCGCGTCGCCTGCTTCGCGGGCAAGCCCGCTCCTACAAGGCCCCTGTAGGAGCGGGCTTGCCCGCGAATGCCCCTCaaa < 2:311625/150‑1 (MQ=255) tCGACTGACCGTGGCGACTATGTGATTTCTTACGCTCTGCCGTAAAACCGCGTCGCCTGCTTCGCGGGCAAGCCCGCTCCTACAAGGCCCCTGTAGGAGCGGGCTTGCCCGCGAATGCCCCTCAAAGACAACCCGAGATTCTGTAATGcc < 1:326123/150‑1 (MQ=255) aTTTCTTACGCTCTGCCGTAAAACCGCGTCGCCTGCTTCGCGGGCAAGCCCGCTCCTACAGGGGCCTTGTAGGAGCgg > 2:504379/1‑78 (MQ=21) aTTTCTTACGCTCTGCCGTAAAACCGCGTCGCCTGCTTCGCGGGCAAGCCCGCTCCTACAGGGGCCTTGTAGGAGCgg < 1:504379/78‑1 (MQ=21) gCCTGCTTCGCGGGCAAGCCCGCTCCTACAAGGCCCCTGTAGGAGCGGGCTTGCCCGCGAATGCCCCTCAAAGACAACCCGAGATTCTGTAATGCCATCCGCAATTCGCATCACCCCCACCCTGCTGCTGGCCCTGGCCAGCACCACTGc > 2:430477/1‑150 (MQ=255) ccGCTCCTACAAGGCCCCTGTAGGAGCGGGCTTGCCCGCGAATGCCCCTCAAAGACAACCCGAGATTCTGTAATGCCATCCGCAATTCGCATCACCCCCa > 1:170510/1‑100 (MQ=255) ccGCTCCTACAAGGCCCCTGTAGGAGCGGGCTTGCCCGCGAATGCCCCTCAAAGACAACCCGAGATTCTGTAATGCCATCCGCAATTCGCATCACCCCCa < 2:170510/100‑1 (MQ=255) tCCTACAGGGGCCTTGTAGGAGCGGGCTTGCCCGCGAATGCCCCTCAAAGACAACCCGAGAtt > 1:19137/1‑63 (MQ=17) tCCTACAGGGGCCTTGTAGGAGCGGGCTTGCCCGCGAATGCCCCTCAAAGACAACCCGAGAtt < 2:19137/63‑1 (MQ=17) tCCTACAAGGCCTCTGTAGGAGCGGGCTTGCCCGCGAATGCTCCTCAAAGACAACCCGAGCTTCTGTAATGCCATCCGCAATTCGCATCACCCCCACCCTGCTGCTGGCCCTGGCCAGCCCCACTGCCCTTGCCAGCGACGATCTCATGa > 2:168003/1‑150 (MQ=255) tCCTACAAGGCCCCTGTAGGAGCGGGCTTGCCCGCGAATGCCCCTCAAAGACAACCCGAGATTCTGTAATGCCATCCGCAATTCGCATCACCCCCACCCTGCTGCTGGCCCTGGCCAGCACCACTGCCCTTGCCAGCGACGATCTCATGa > 2:168083/1‑150 (MQ=255) gCCCCTGTAGGAGCGGGCTTGCCCGCGAATGCCCCTCAAAGACa > 2:216630/1‑44 (MQ=255) gCCCCTGTAGGAGCGGGCTTGCCCGCGAATGCCCCTCAAAGACa < 1:216630/44‑1 (MQ=255) | CCTACGTCTCGCCGAAGACCGGCAAGCAGTACGTGGTGATCACTGCTGGCGGCGCCCGCCAGTCGACTGACCGTGGCGACTATGTGATTTCTTACGCTCTGCCGTAAAACCGCGTCGCCTGCTTCGCGGGCAAGCCCGCTCCTACAAGGCCCCTGTAGGAGCGGGCTTGCCCGCGAATGCCCCTCAAAGACAACCCGAGATTCTGTAATGCCATCCGCAATTCGCATCACCCCCACCCTGCTGCTGGCCCTGGCCAGCACCACTGCCCTTGCCAGCGACGATCTCATGA > NC_002947/4049805‑4050093 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |