| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 4,091,497 | T→A | 20.6% | V21D (GTC→GAC) | garD → | galactarate dehydratase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 4,091,497 | 0 | T | A | 20.6% | 34.0 / 6.8 | 20 | V21D (GTC→GAC) | garD | galactarate dehydratase |
| Reads supporting (aligned to +/- strand): ref base T (11/4); new base A (2/2); total (13/7) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.57e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.38e-01 | |||||||||||
TGAAAAAACACGCCCGCGCCGGGTCGCCCGGCATGGGCGGATGTACAAAAGCCTGAGAACCTGACAAGTAGGGCCCAAACATGCAGTTGATCGAACATTCCGACTCGCCCCGCT‑ACGTCCGCCTGCACGACGACGACAACGTCGTGGTCGTCGTCAACGACGGCGGCTTGGGCGAAGGCGCCCGCTTCGCCGACGGCCTGACCCTGATCGAAGGCGTGCCGCAAAGCCATAAGGTGGCCACCGTGTCCATCGCCAAGGGCGAGCCCGTGCGCCGCTACGGGC > NC_002947/4091356‑4091637 | tGAAAAAACACGCCCGCGCCGGGTCGCCCGGCATGGGCGGATGTACAAAAGCCGGAGAACCTGACAAGTAGGGCCCCAACATGCAGTTGATCGAACATTCCGCCTCGCCCACCG‑ACGTCCGACTGCACGCCGCCGACAACGTCGTGgtcg > 1:133544/1‑150 (MQ=255) tccGCCCGCGCCGGGTCGCCCGGCATGGGCGGATGTACAAAAGCCTGAGAACCTGACAAGTAGGGCCCAAACATGCAGTTGATCGAACATTCCGACTCGCCCCGCT‑ACGTCCGCCTGCACGACGACGACAACGTCGTGGTCGTCGTCAac > 1:439610/3‑150 (MQ=255) aCAAAAGCCTGAGAACCTGACAAGTAGGGCCCAAACATGCAGTTGACAGCACCTCAAGACTAGCCACGCTCAGCGCCACCGGTTTG‑CGACGACAACGGCGGGGTTGTCGTCAACGACGGCTGCTTGGGCGAAGGCGCCCGCTTCGCCGAc < 2:439610/150‑1 (MQ=255) aaaGCCTGAGAACCTGACAAGTAGGGCCCAAACATGCAGTTGATCGAACATTCCGACTCGCCCCGCT‑ACGTCCGCCTGCACGACGACGACAACGTCGTGGTCGTCGTCAACGACGGCGGCTTGGGCGAAGGCGCCCGCTTCGCCGACGGc > 2:400703/1‑150 (MQ=255) aCCTGACAAGTAGGGCCCAAACATGCAGTTGATCGAACATTCCGACTCGCCCCGCT‑ACGTCCGCCTGCACGACGACGACAACGTCGTGGTCGTCGTCAACGACGGCGGCTTGGGCGAAGgcgc > 1:389630/1‑123 (MQ=255) aCCTGACAAGTAGGGCCCAAACATGCAGTTGATCGAACATTCCGACTCGCCCCGCT‑ACGTCCGCCTGCACGACGACGACAACGTCGTGGTCGTCGTCAACGACGGCGGCTTGGGCGAAGgcgc < 2:389630/123‑1 (MQ=255) cTGACAAGTAGGGCCCAAACATGCAGTTGATCGAACATTCCGACTCGCCCCGCT‑ACGTCCGCCTGCACGACGACGACAACGTCGTGGTCGTCGTCAACGACGGCGGCTTGGGCGAAGGCGCCCGCTTCGCCGACGGCCTGACCCTGATCg > 2:35517/1‑150 (MQ=255) gggCCCAAACATGCAGTTGATCGAACATTCCGACTCGCCCCGCT‑ACGTCCGCCTGCACGACGACGACAACGTCGTGGTCGTCGTCAACGACGGCGGCTTGGGCGAAGGCGCCCGCTTCGCCGACGGCCTGACCCTGATCGAAGGCGTGcc > 1:3809/1‑150 (MQ=255) ttGATCGAACATTCCGACTCGCCCCGCT‑ACGTCCGCCTGCACGACGACGACAACGTCGTGGTCGTCGTCAACGACGGCGGCTTGGGCGAAGGCGCCCGCTTCGCCGACGGCCTGACCCTGATCGAAGGCGTGCCGCAAAGCCATa < 1:29553/145‑1 (MQ=255) ttGATCGAACATTCCGACTCGCCCCGCT‑ACGTCCGCCTGCACGACGACGACAACGTCGTGGTCGTCGTCAACGACGGCGGCTTGGGCGAAGGCGCCCGCTTCGCCGACGGCCTGACCCTGATCGAAGGCGTGCCGCAAAGCCATa > 2:29553/1‑145 (MQ=255) gAACATTCCGACTCGCCCCGCT‑ACGTCCGCCTGCACGACGACGACAACGTCGTGGTCGTCGTCAACGACGGCGGCTTGGGCGAAGGCGCCCGCTTCGCCGACGGCCTGACCCTGATCGAAGGCGTGCCGCAAAGCCATAAGGTGGCCAcc < 2:3809/150‑1 (MQ=255) aCATTCCGACTCGCCCCGCT‑ACGTCCGCCTGCACGACGACGACAACGTCGTGGTCGTCGTCAACGACGGCGGCTTGGGCGAAGGCGCCCGCTTCGCCGACGGCCTGACCCTGATCGAAGGCGTGCCGCAAAGCCATAAGGTGGCCACCgt > 2:50419/1‑150 (MQ=255) tCGCCCCGCT‑ACGTCCGCCTGCACGACGACGACAACGTCGTGGTCGTCGTCAACGACGGCGGCTTGGGCGAAGGCGCCCGCTTCGCCGACGGCCTGACCCTGATCGAAGGCGTGCCGCAAAGCCATAAGGTGGCCACCGTGTCCATCGcc > 2:125303/1‑150 (MQ=255) gacgacgacAACGTCGTGGTCGTCGTCAACGACGGCGGCTTGGGCGAAGGCGCCCGCTTCGCCGACGGCCTGACCCTGAt > 1:477620/1‑80 (MQ=255) gacgacgacAACGTCGTGGTCGTCGTCAACGACGGCGGCTTGGGCGAAGGCGCCCGCTTCGCCGACGGCCTGACCCTGAt < 2:477620/80‑1 (MQ=255) cgacgacCACGACGTTGTCGTCGTCAACGACGGCGGCTTGGGCGAAGGCGCCCGCTTCGCCGACGGCCTGACCCTGATCGAAGGc < 1:477664/85‑1 (MQ=38) cgacgacCACGACGTTGTCGTCGTCAACGACGGCGGCTTGGGCGAAGGCGCCCGCTTCGCCGACGGCCTGACCCTGATCGAAGGc > 2:477664/1‑85 (MQ=38) gacgacCACGACGTTGTCGTCGTCAACGACGGCGGCTTGGGCGAAGGCGCCCGCTTCGCCGACGGCCTGACCCTGATCGAAGGCGTGCCGCAAAGCCATAAGGTGGCCACCGTGTCCATCGCCAAGGGCGAGCCCGTGCGCCGCTACgg < 2:420410/149‑1 (MQ=255) gacgacCACGACGTTGTCGTCGTCAACGACGGCGGCTTGGGCGAAGGCGCCCGCTTCGCCGACGGCCTGACCCTGATCGAAGGCGTGCCGCAAAGCCATAAGGTGGCCACCGTGTCCATCGCCAAGGGCGAGCCCGTGCGCCGCTACgg > 1:420410/1‑149 (MQ=255) acgacAACGTCGTGGTCGTCGTCAACGACGGCGGCTTGGGCGAAGGCGCCCGCTTCGCCGACGGCCTGACCCTGATCGAAGGCGTGCCGCAAAGCCATAAGGTGGCCACCGTGTCCATCGCCAAGGGCGAGCCCGTGCGCCGCTACGGGc > 1:197911/1‑150 (MQ=255) | TGAAAAAACACGCCCGCGCCGGGTCGCCCGGCATGGGCGGATGTACAAAAGCCTGAGAACCTGACAAGTAGGGCCCAAACATGCAGTTGATCGAACATTCCGACTCGCCCCGCT‑ACGTCCGCCTGCACGACGACGACAACGTCGTGGTCGTCGTCAACGACGGCGGCTTGGGCGAAGGCGCCCGCTTCGCCGACGGCCTGACCCTGATCGAAGGCGTGCCGCAAAGCCATAAGGTGGCCACCGTGTCCATCGCCAAGGGCGAGCCCGTGCGCCGCTACGGGC > NC_002947/4091356‑4091637 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |