| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 4,119,525 | T→G | 16.0% | V724G (GTG→GGG) | PP_3622 → | isoquinoline 1‑oxidoreductase subunit beta |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 4,119,525 | 0 | T | G | 16.0% | 50.7 / 6.5 | 25 | V724G (GTG→GGG) | PP_3622 | isoquinoline 1‑oxidoreductase subunit beta |
| Reads supporting (aligned to +/- strand): ref base T (12/9); new base G (2/2); total (14/11) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.72e-01 | |||||||||||
TACGTGGATGGCAAGCCGCGCGCACGCAATTACGACCTGTACCCGATCCTGCCGCCTTCGCGGATGGCGCGGGTGCATGTGCGCATCATCGAAAGCGGGGCGAAGATGGGCGGCATCGGCGAACCACCGCTGCCCGCCGTGGCCCCGGCGGTGGCCAACGCGGTCGCGCAGCTCACTGGCCAGCGCGTGCGCAGTTTGCCATTGAGCCGCCACACCTTCAGCTGATCGCACAAGGGACGCCTCATGACCCATCGTTTCGCAAGAACCGCTGGCTGGC > NC_002947/4119386‑4119662 | tACGTGGATGGCAAGCCGCGCGCACGCAATTACGACCTGTACCCGATCCTGCCGCCTTCGCGGATGGCGCCGGTGCATGTGCGCATCATCGAAAGCGGGGCGAAGATGGGCGGCATCGGCGAACCACCGCTGCCCGCCGTGGCCCcggcg > 1:48300/1‑150 (MQ=255) acgcaATTACGACCTGTACCCGATCCTGCCGCCTTCGCGGATGGCGCGGGTGCATGTGCGCATCATCGAAAGCGGGGCGAAGATGGGCGGCATCGGCGAACCACCGCTGCCCGCCGTGGCCCCGGCGGTGGCCAACGCGGTCgcgctggc > 2:165349/1‑146 (MQ=255) acgcaATTACGACCTGTACCCGATCCTGCCGCCTTCGCGGATGGCGCGGGTGCATGTGCGCATCATCGAAAGCGGGGCGAAGATGGGCGGCATCGGCGAACCACCGCTGCCCGCCGTGGCCCCGGCGGTGGCCAACGCGGTCGCGCAGCt > 2:302828/1‑150 (MQ=255) gcaATTACGACCTGTACCCGATCCTGCCGCCTTCGCGGATGGGGTGGCTGTATGTGCGCATCATCGAAATCGGTTCGAAGATGGGCGGCATCGGCGAACCACCGCTGCCCGCCGTGGCCCCGGCGGTGGCCAACGCGGTCGCGCAGCTCa > 1:138666/1‑150 (MQ=255) gTACCCGATCCTGCCGCCTTCGCGGATGGCCCGTCCGTATTTGCGCATCATCGAAAGCGGGGCGAAGATGGGCGGCATCGGCGAACCACCGCTGCCCGCCGTGGCCCCGGCGGTGGCCAACGCGGTCGCGCAGCTCACTGGCCAGCGCGt < 2:120258/150‑1 (MQ=255) cgccTTCGCGGATGGCGCGGGTGCATGTGCGCATCATCGAAAGCGGGGCGAAGATGGGCGGCATCGGCGAACCACCGCTGCCCGCCGTGGCCCCGGCGGTGGCCAACGCGGTCGCGCAGCTCACTGGCCAgcgc > 2:446900/1‑134 (MQ=255) cgccTTCGCGGATGGCGCGGGTGCATGTGCGCATCATCGAAAGCGGGGCGAAGATGGGCGGCATCGGCGAACCACCGCTGCCCGCCGTGGCCCCGGCGGTGGCCAACGCGGTCGCGCAGCTCACTGGCCAgcgc < 1:446900/134‑1 (MQ=255) gccTTCGCGGATGGCGCGGGTGCATGTGCGCATCATCGAAAGCGGGGCGAAGATGGGCGGCATCGGCGAACCACCGCTGCCCGCCGTGGCCCCGGCGGTGGCCAACGCGGTCGCGCAGCTCACTGGCCAGCGCGTGCGCAGTTTGCCAtt < 2:138666/150‑1 (MQ=255) ttCGCGGATGGCGCGGGTGCATGTGCGCATCATCGAAAGCGGGGCGAAGATGGGCGGCATCGGCGAACCACCGCTGCCCGCCGTGGCCCCGGCGGTGGCCAACGCGGTcgca < 2:404231/112‑2 (MQ=255) ttCGCGGATGGCGCGGGTGCATGTGCGCATCATCGAAAGCGGGGCGAAGATGGGCGGCATCGGCGAACCACCGCTGCCCGCCGTGGCCCCGGCGGTGGCCAACGCGGTcgca > 1:404231/1‑111 (MQ=255) aTGGCGCGGGTGCATGTGCGCATCATCGAAAGCGGGGCGAAGATGGGCGGCATCGGCGAACCACCGCTGCCCGCCGTGGCCCCGGCGGTGGCCAACGCGGTCGCGCAGCTCACTGGCCAGCGCGTGCGCAGTTTGCCAtt > 1:221541/1‑140 (MQ=255) aTGGCGCGGGTGCATGTGCGCATCATCGAAAGCGGGGCGAAGATGGGCGGCATCGGCGAACCACCGCTGCCCGCCGTGGCCCCGGCGGTGGCCAACGCGGTCGCGCAGCTCACTGGCCAGCGCGTGCGCAGTTTGCCAtt < 2:221541/140‑1 (MQ=255) tcatcGAAAGCGGGGCGAAGATGGGCGGCATCGGCGAACCACCGCTGCCCGCCGTGGCCCCGGCGGTGGCCAACGCGGTCGCGCAGCTCACTGGCCAGCGCGTGCGCAGTTTGCCATTGAGCCGCCACACCTTCAGCTGATCGCACAAgg < 2:48300/150‑1 (MQ=255) ggCGAAGATGGGCGGCATCGGCGAACCACCGCTGCCCGCCGTGGCCCCGGCGGTGGCCAACGCGGTCGCGCAGCTCACTGGCCAGCGCGTGCGCAGTTTGCCATTGAGCCGCCACACCTTCAGCTGATCGCACAAGGGACGCCTCATGAc < 1:290260/150‑1 (MQ=255) gAAGATGGGCGGCATCGGCGAACCACCGCTGCCCGCCGTGGCCCCGGCGGTGGCCAACGCGGTCGCGCAGCTCACTGGCCAGCGCGTGCGCAGTTTGCCATTGAGCCGCCACACCTTCAGCTGATCGCACAAGGGa > 1:423477/1‑136 (MQ=255) gAAGATGGGCGGCATCGGCGAACCACCGCTGCCCGCCGTGGCCCCGGCGGTGGCCAACGCGGTCGCGCAGCTCACTGGCCAGCGCGTGCGCAGTTTGCCATTGAGCCGCCACACCTTCAGCTGATCGCACAAGGGa < 2:423477/136‑1 (MQ=255) gAAGATGGGCGGCATCGGCGAACCACCGCTGCCCGCCGTGGCCCCGGCGGTGGCCAACGCGGTCGCGCAGCTCACTGGCCAGCGCGTGCGCAGTTTGCCATTGAGCCGCCACACCTTCAGCTGATCGCACAAGGGACGCCTCATGACCCa > 1:236492/1‑150 (MQ=255) gAACCACCGCTGCCCGCCGTGGCCCCGGCGGTGGCCAACGCGGTCGCGCAGCTCACTGGCCAGCGCGTGCGCAGTTTGCCATTGAGCCGCCACACCTTCAGCTGATCGCACAAGGGACGCCTCATGACCCATCGTTTCGCAAGAACCgct > 2:48114/1‑150 (MQ=255) caccGCTGCCCGCCGTGGCCCCGGCGGTGGCCAACGCGGTCGCGCAGCTCACTGGCCAGCGCGTGCGCAGTTTGCCATTGAGCCGCCACACCTTCAGCTGATCGCACAAGGGACGCCTCATGACCCATCGTTTCGCAAGAACCgctggct > 1:200647/1‑150 (MQ=255) accGCTGCCCGCCGTGGCCCCGGCGGTGGCCAACGCGGTCGCGCAGCTCACTGGCCAGCGCGTGCGCAGTTTGCCATTGAGCCGCCACACCTTCAGCTGATCGCACAAGGGACGCCTCATGACCCATCGTTTCGCAAGAACCgctggctg > 1:162708/1‑150 (MQ=255) cGCTGCCCGCCGTGGCCCCGGCGGTGGCCAACGCGGTCGCGCAGCTCACTGGCCAGCGCGTGCGCAGTTTGCCATTGAGCCGCCACACCTTCAGCTGATCGCACAAGGGACGCCTCATGACCCATCGTTTCGCAAGAACCgctggctggc < 1:302828/150‑1 (MQ=255) gccaccgccgGGGCCACGGCGGTGGCCAACGCGGTCGCGCAGCTCACTGGCCAGCGCGTGCGCAGTTTGCCATTGAGCCGCCACACCTTCAGCTGATCGCACAAGGGACGCCTCATGACCCATCGTTTCGCAAGAAcc < 2:178414/134‑1 (MQ=255) gccaccgccgGGGCCACGGCGGTGGCCAACGCGGTCGCGCAGCTCACTGGCCAGCGCGTGCGCAGTTTGCCATTGAGCCGCCACACCTTCAGCTGATCGCACAAGGGACGCCTCATGACCCATCGTTTCGCAAGAAcc > 1:178414/5‑138 (MQ=255) accgccgGGGCCACGGCGGTGGCCAACGCGGTCGCGCAGCTCACTGGCCAGCGCGTGCGCAGTTTGCCATTGAGCCGCCACACCTTCAGc < 2:187898/89‑1 (MQ=255) accgccgGGGCCACGGCGGTGGCCAACGCGGTCGCGCAGCTCACTGGCCAGCGCGTGCGCAGTTTGCCATTGAGCCGCCACACCTTCAGc > 1:187898/2‑90 (MQ=255) | TACGTGGATGGCAAGCCGCGCGCACGCAATTACGACCTGTACCCGATCCTGCCGCCTTCGCGGATGGCGCGGGTGCATGTGCGCATCATCGAAAGCGGGGCGAAGATGGGCGGCATCGGCGAACCACCGCTGCCCGCCGTGGCCCCGGCGGTGGCCAACGCGGTCGCGCAGCTCACTGGCCAGCGCGTGCGCAGTTTGCCATTGAGCCGCCACACCTTCAGCTGATCGCACAAGGGACGCCTCATGACCCATCGTTTCGCAAGAACCGCTGGCTGGC > NC_002947/4119386‑4119662 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |