Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	4,159,109	T→C	13.3%	intergenic (+13/‑196)	PP_3661 → / → PP_3662	membrane protein/hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	4,159,109	0	T	C	13.3%	77.4 / 2.8	30	intergenic (+13/‑196)	PP_3661/PP_3662	membrane protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (10/16);  new base C (2/2);  total (12/18)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.90e-01

CATGGAGACCCGCTTCAGCCAGATTCGCCAGGCTGGGCCACGGGCGCTGGCCACCGGTGCGATCCTCAACCTGTGGCTGGTTGGCGGGGGGCTGGCGATTACCCTGGGGGTGCAGAAGCTGTTGGGCTGACTGGAGACCGAGTCGGCTGCTTCGCGGGTAAACCCGCTCCTACAGGTGGGTGCTGCCCTGAAGACGGCAAGGCCCCTGTAGGAGCGGGTTCACCCGCGAAGCAGGCAGCTCGGTCTCAAGGTTTTTCACAGCCCCCCTCGTTGCCGTCCACATTTCT  >  NC_002947/4158967‑4159253                                                                                                                                               |                                                                                                                                                 cATGGAGACCCGCTTCAGCCAGATTCGCCAGGCTGGGCCACGGGAGATAGCGACCTGTGCGATCCTCAACCTGTGGCTGGTTGGCGGGGGGCTGGCGATTACCCTGGGGGTGCAGAAGCTGTTGGGCTGACTGGAGACCGAGTCGgctgc                                                                                                                                           <  1:31253/150‑1 (MQ=255)          ccGCTTCAGCCAGATTCGCCAGGCTGGGCCACGGGCGCTGGCCACCGGTGCGATCCTCAACCTGTGGCTGGTTGGCGGGGGGCTGGCGATTACCCTGGGGGTGCAGAAGCTGTTGGGCTGACTGGAGACCGAGTCGGCTGCTTCGCgg                                                                                                                                    <  1:274731/148‑1 (MQ=255)          ccGCTGCAGCCAGATTCGCCAGGCTGGGCCACGGGCGCTGGCCACCGGTGCGATCCTCAACCTGTGGCTGGTTGGCGGGGGGCTGGCGATTACCCTGGGGGTGCAGAAGCTGTTGGGCTGACTGGAGACCGAGTCGGCTGCTTCGCgg                                                                                                                                    >  2:274731/1‑148 (MQ=255)               tCAGCCAGATTCGCCAGGCTGGGCCACGGGCGCTGGCCACCGGTGCGATCCTCAACCTGTGGCTGGTTGGCGGGGGGCTGGCGATTACCCTGGGGGTGCAGAAGCTGTTGGGCTGACTGGAGACCGAGTCGGCTGCTTCGCGGGTAAAcc                                                                                                                             <  2:163880/150‑1 (MQ=255)                 aGCCAGATTCGCCAGGCTGGGCCACGGGCGCTGGCCACCGGTGCGATCCTCAACCTGTGGCTGGTTGGCGGGGGGCTGGCGATTACCCTGGGGGTGCAGAAGCTGTTGGGCTGACTGGAGACCGAGTCGGCTGCTTCGCGGGTAAACCCg                                                                                                                           >  1:405229/1‑150 (MQ=255)                  gCCAGATTCGCCAGGCTGGGCCACGGGCGCTGGCCACCGGTGCGATCCTCAACCTGTGGCTGGTTGGCGGGGGGCTGGCGATTACCCTGGGGGTGCAGAAGCTGTTGGGCTGGCTGGAGACCGAGTCGGCTGCTTCGCGGGTAAACCCGc                                                                                                                          <  1:459788/150‑1 (MQ=255)                         tCGCCAGGCTGGGCCACGGGCGCTGGCCACCGGTGCGATCCTCAACCTGTGGCTGGTTGGCGGGGGGCTGGCGATTACCCTGGGGGTGCAGAAGCTGTTGGGCTGACTGGAGACCGAGTCGGCTGCTTCGCGGGTAAACCCGCTCCTACa                                                                                                                   <  2:19294/150‑1 (MQ=255)                           gCCAGGCTGGGCCACGGGCGCTGGCCACCGGTGCGATCCTCAACCTGTGGCTGGTTGGCGGGGGGCTGGCGATTACCCTGGGGGTGCAGAAGCTGTTGGGCTGACTGGAGACCGAGTCGGCTGCTTCGCGGGTAAACCCGCTCCTACAgg                                                                                                                 >  2:239984/1‑150 (MQ=255)                                gCTGGGCCACGGGCGCTGGCCACCGGTGCGATCCTCAACCTGTGGCTGGTTGGCGGGGGGCTGGCGATTACCCTGGGGGTGCAGAAGCTGTTGGGCTGACTGGAGACCGAGTCGGCTGCTTCGCGGGTAAACCCGATCCTACAggtgggt                                                                                                            <  2:85685/150‑1 (MQ=255)                                               cTGGCCACCGGTGCGATCCTCAACCTGTGGCTGGTTGGCGGGGGGCTGGCGATTACCCTGGGGGTGCAGAAGCTGTTGGGCTGACTGGAGACCGAGTCGGCTGCTTCGCGGGTAAACCCGCTCCTACAGGTGGGTGCTGCCCTGAAGACg                                                                                             <  1:464937/150‑1 (MQ=255)                                                             gATCCTCAACCTGTGGCTGGTTGGCGGGGGGCTGGCGATTACCCTGGGGGTGCAGAAGCTGTTGGGCTGACTGGAGACCGAGTCGGCTGCTTCGCGGGTAAACCCGCTCCTACAGGTGGGTGCTGCCCTGAAGACGGCAAGGccc                                                                                    >  2:41378/1‑145 (MQ=255)                                                             gATCCTCAACCTGTGGCTGGTTGGCGGGGGGCTGGCGATTACCCTGGGGGTGCAGAAGCTGTTGGGCTGACTGGAGACCGAGTCGGCTGCTTCGCGGGTAAACCCGCTCCTACAGGTGGGTGCTGCCCTGAAGACGGCAAGGccc                                                                                    <  1:41378/145‑1 (MQ=255)                                                                      ccTGTGGCTGGTTGGCGGGGGGCTGGCGATTACCCTGGGGGTGCAGAAGCTGTTGGGCTGACTGGAGACCGAGTCGGCTGCTTCGCGGGTAAACCCGCTCCTACAGGTGGGTGCTGCCCTGAAGACGGCAAGGCCCCTGTAGGa                                                                            <  2:111298/144‑1 (MQ=255)                                                                      ccTGTGGCTGGTTGGCGGGGGGCTGGCGATTACCCTGGGGGTGCAGAAGCTGTTGGGCTGACTGGAGACCGAGTCGGCTGCTTCGCGGGTAAACCCGCTCCTACAGGTGGGTGCTGCCCTGAAGACGGCAAGGCCCCTGTAGGa                                                                            >  1:111298/1‑144 (MQ=255)                                                                            gCTGGTTGGCGGGGGGCTGGCGATTACCCTGGGGGTGCAGAAGCTGTTGGGCTGACTGGAGACCGAGTCGGCTGCTTCGCGGGTAAACCCGCTCCTACAGGTGGGTGCTGCCCTGAAGACGGCAAGGCCCCTGTAGGAGCGGGTTCAccc                                                                >  2:330951/1‑150 (MQ=255)                                                                              tGGTTGGCGGGGGGCTGGAGATTAACATCGGGGTGCAGAAGCTGTTTTGCTGACTGGAGACCGAGTCGGCTGCTTCGCGGGTAAACCCGCTCCTACAGGTGGGTGCTGCCCTGAAGACGGCAAGGCCCCTGTAGGAGCGGGTTCACCcgc                                                              <  2:387123/150‑1 (MQ=38)                                                                                               gCGATTCCCCTGGGGGTGCAGAAGCTGTTGTGCTGATAGGAGAGCGAGTCGGCTGTTTCGCGGGTGAACCCGCTCCTACAGGTGGGTGCTGCCCTCAAGACGGCAAGGTCCCTGTAGGAGCGGGTTCACCCGCGAAGCAGGCAGCTCGGt                                             <  2:163103/150‑1 (MQ=255)                                                                                                  aTTACCCTGGGGGTGCAGAAGCTGTTGGGCTGACTGGAGACCGAGTCGGCTGCTTCGCGGGTAAACCCGCTCCTACAGGTGGGTGCTGCCCTGAAGACGGCAAGGCCCCTGTAGGAGCggg                                                                       >  2:161134/1‑121 (MQ=255)                                                                                                  aTTACCCTGGGGGTGCAGAAGCTGTTGGGCTGACTGGAGACCGAGTCGGCTGCTTCGCGGGTAAACCCGCTCCTACAGGTGGGTGCTGCCCTGAAGACGGCAAGGCCCCTGTAGGAGCggg                                                                       <  1:161134/121‑1 (MQ=255)                                                                                                   ttACCCTGGGGGTGCAGAAGCTGTTGGGCTGACTGGAGACCGAGTCGGCTGCTTCGCGGGTAAACCCGCTCCTACAGGTGGGTGCTGCCCTGAAGACGGCAAGGCCCCTGTAGGAGCGGGTTCACCCGCGAAGCAGGCAGCTCGGTCTCa                                         <  2:508081/150‑1 (MQ=255)                                                                                                                   gAAGCTGTTGGGCTGACTGGAGACCGAGTCGGCTGCTTCGCGGGTAAACCCGCTCCTACAGGTGGGTGCTGCCCTGAAGACGGCAAGGCCCCTGTAGGAGCGGGTTCACCCGCGAAGCAGGCAGCTCGGTCTCAAGGTTTTTCACAGccc                         >  1:158675/1‑150 (MQ=255)                                                                                                                           tGGGCTGACTGGAGACCGAGTCGGCTGCTTCGCGGGTAAACCCGCTCCTACAGGTGGGTGCTGCCCTGAAGACGGCAAGGCCCCTGTAGGAGCGGGTTCACCCGCGAAGCAGGCAGCTCGGTCTCAAGGTTTTTCACAGCCCCCCTCGtt                 <  2:391819/150‑1 (MQ=255)                                                                                                                             gtcTGACTGGAGACCGAGTCGGCTGCTTCGCGGGTAAACCAGCTCATACCGGCGGGTGCTGCCCTGAAGACGTCAAGGCCACTGTCGAAACGGATTCATTCTTTAAGCAGGCAGCTCGGTCTCAAGGTTTTTCACAGCCCCCCTCGTTga               >  1:391819/3‑149 (MQ=255)                                                                                                                                    ttgagaCCGAGCTGCCTGCTTCGCGGGTGAACCCGCTCCTACAGGTGGGCGCTGCCCTGAAGACGGCAAGGCCCCTGTAGGa                                                                            >  2:191310/3‑82 (MQ=17)                                                                                                                                    ttgagaCCGAGCTGCCTGCTTCGCGGGTGAACCCGCTCCTACAGGTGGGCGCTGCCCTGAAGACGGCAAGGCCCCTGTAGGa                                                                            >  2:191311/3‑82 (MQ=17)                                                                                                                                    ttgagaCCGAGCTGCCTGCTTCGCGGGTGAACCCGCTCCTACAGGTGGGCGCTGCCCTGAAGACGGCAAGGCCCCTGTAGGa                                                                            <  1:191311/80‑1 (MQ=18)                                                                                                                                    ttgagaCCGAGCTGCCTGCTTCGCGGGTGAACCCGCTCCTACAGGTGGGCGCTGCCCTGAAGACGGCAAGGCCCCTGTAGGa                                                                            <  1:191310/80‑1 (MQ=17)                                                                                                                                          ccGAGTCGGCTGCTTCGCGGGTAAACCCGCTCCTACAGGTGGGTGCTGCCCTGAAGACGGCAAGGCCCCTGTAGGAGCGGGTTCACCCGCGAAGCAGGCAGCTCGGTCTCAAGGTTTTTCACAGCCCCCCTCGTTGCCGTCCACATTtct  <  2:405229/150‑1 (MQ=255)                                                                                                                                             aGTCGGCTGCTTCGCGGGTAAACCCGCTCCTACAGGTGGGTGCTGCCCTGAAGACGGCAAGGCCCCTGTAGGAGCGGGTTCACCCGCGAAGCAGGCAGCTCGGTCTCAAGGTTTTTCACAGCCCCCCTCGTTg                <  1:76112/133‑1 (MQ=255)                                                                                                                                             aGTCGGCTGCTTCGCGGGTAAACCCGCTCCTACAGGTGGGTGCTGCCCTGAAGACGGCAAGGCCCCTGTAGGAGCGGGTTCACCCGCGAAGCAGGCAGCTCGGTCTCAAGGTTTTTCACAGCCCCCCTCGTTg                >  2:76112/1‑133 (MQ=255)                                                                                                                                               |                                                                                                                                                 CATGGAGACCCGCTTCAGCCAGATTCGCCAGGCTGGGCCACGGGCGCTGGCCACCGGTGCGATCCTCAACCTGTGGCTGGTTGGCGGGGGGCTGGCGATTACCCTGGGGGTGCAGAAGCTGTTGGGCTGACTGGAGACCGAGTCGGCTGCTTCGCGGGTAAACCCGCTCCTACAGGTGGGTGCTGCCCTGAAGACGGCAAGGCCCCTGTAGGAGCGGGTTCACCCGCGAAGCAGGCAGCTCGGTCTCAAGGTTTTTCACAGCCCCCCTCGTTGCCGTCCACATTTCT  >  NC_002947/4158967‑4159253

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑