| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 4,159,126 | A→G | 16.9% | intergenic (+30/‑179) | PP_3661 → / → PP_3662 | membrane protein/hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 4,159,126 | 0 | A | G | 16.9% | 63.4 / 4.3 | 29 | intergenic (+30/‑179) | PP_3661/PP_3662 | membrane protein/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base A (10/14); new base G (2/3); total (12/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TCAGCCAGATTCGCCAGGCTGGGCCACGGGCGCTGGCCACCGGTGCGATCCTCAACCTGTGGCTGGTTGGCGGGGGGCTGGCGATTACCCTGGGGGTGCAGAAGCTGTTGGGCTGACTGGAGACCGAGTCGGCTGCTTCGCGGGTAAACCCGCTCCTACAGGTGGGTGCTGCCCTGAAGACGGCAAGGCCCCTGTAGGAGCGGGTTCACCCGCGAAGCAGGCAGCTCGGTCTCAAGGTTTTTCACAGCCCCCCTCGTTGCCGTCCACATTTCT > NC_002947/4158981‑4159253 | tCAGCCAGATTCGCCAGGCTGGGCCACGGGCGCTGGCCACCGGTGCGATCCTCAACCTGTGGCTGGTTGGCGGGGGGCTGGCGATTACCCTGGGGGTGCAGAAGCTGTTGGGCTGACTGGAGACCGAGTCGGCTGCTTCGCGGGTAAAcc < 2:163880/150‑1 (MQ=255) aGCCAGATTCGCCAGGCTGGGCCACGGGCGCTGGCCACCGGTGCGATCCTCAACCTGTGGCTGGTTGGCGGGGGGCTGGCGATTACCCTGGGGGTGCAGAAGCTGTTGGGCTGACTGGAGACCGAGTCGGCTGCTTCGCGGGTAAACCCg > 1:405229/1‑150 (MQ=255) gCCAGATTCGCCAGGCTGGGCCACGGGCGCTGGCCACCGGTGCGATCCTCAACCTGTGGCTGGTTGGCGGGGGGCTGGCGATTACCCTGGGGGTGCAGAAGCTGTTGGGCTGGCTGGAGACCGAGTCGGCTGCTTCGCGGGTAAACCCGc < 1:459788/150‑1 (MQ=255) tCGCCAGGCTGGGCCACGGGCGCTGGCCACCGGTGCGATCCTCAACCTGTGGCTGGTTGGCGGGGGGCTGGCGATTACCCTGGGGGTGCAGAAGCTGTTGGGCTGACTGGAGACCGAGTCGGCTGCTTCGCGGGTAAACCCGCTCCTACa < 2:19294/150‑1 (MQ=255) gCCAGGCTGGGCCACGGGCGCTGGCCACCGGTGCGATCCTCAACCTGTGGCTGGTTGGCGGGGGGCTGGCGATTACCCTGGGGGTGCAGAAGCTGTTGGGCTGACTGGAGACCGAGTCGGCTGCTTCGCGGGTAAACCCGCTCCTACAgg > 2:239984/1‑150 (MQ=255) gCTGGGCCACGGGCGCTGGCCACCGGTGCGATCCTCAACCTGTGGCTGGTTGGCGGGGGGCTGGCGATTACCCTGGGGGTGCAGAAGCTGTTGGGCTGACTGGAGACCGAGTCGGCTGCTTCGCGGGTAAACCCGATCCTACAggtgggt < 2:85685/150‑1 (MQ=255) cTGGCCACCGGTGCGATCCTCAACCTGTGGCTGGTTGGCGGGGGGCTGGCGATTACCCTGGGGGTGCAGAAGCTGTTGGGCTGACTGGAGACCGAGTCGGCTGCTTCGCGGGTAAACCCGCTCCTACAGGTGGGTGCTGCCCTGAAGACg < 1:464937/150‑1 (MQ=255) gATCCTCAACCTGTGGCTGGTTGGCGGGGGGCTGGCGATTACCCTGGGGGTGCAGAAGCTGTTGGGCTGACTGGAGACCGAGTCGGCTGCTTCGCGGGTAAACCCGCTCCTACAGGTGGGTGCTGCCCTGAAGACGGCAAGGccc > 2:41378/1‑145 (MQ=255) gATCCTCAACCTGTGGCTGGTTGGCGGGGGGCTGGCGATTACCCTGGGGGTGCAGAAGCTGTTGGGCTGACTGGAGACCGAGTCGGCTGCTTCGCGGGTAAACCCGCTCCTACAGGTGGGTGCTGCCCTGAAGACGGCAAGGccc < 1:41378/145‑1 (MQ=255) ccTGTGGCTGGTTGGCGGGGGGCTGGCGATTACCCTGGGGGTGCAGAAGCTGTTGGGCTGACTGGAGACCGAGTCGGCTGCTTCGCGGGTAAACCCGCTCCTACAGGTGGGTGCTGCCCTGAAGACGGCAAGGCCCCTGTAGGa < 2:111298/144‑1 (MQ=255) ccTGTGGCTGGTTGGCGGGGGGCTGGCGATTACCCTGGGGGTGCAGAAGCTGTTGGGCTGACTGGAGACCGAGTCGGCTGCTTCGCGGGTAAACCCGCTCCTACAGGTGGGTGCTGCCCTGAAGACGGCAAGGCCCCTGTAGGa > 1:111298/1‑144 (MQ=255) gCTGGTTGGCGGGGGGCTGGCGATTACCCTGGGGGTGCAGAAGCTGTTGGGCTGACTGGAGACCGAGTCGGCTGCTTCGCGGGTAAACCCGCTCCTACAGGTGGGTGCTGCCCTGAAGACGGCAAGGCCCCTGTAGGAGCGGGTTCAccc > 2:330951/1‑150 (MQ=255) tGGTTGGCGGGGGGCTGGAGATTAACATCGGGGTGCAGAAGCTGTTTTGCTGACTGGAGACCGAGTCGGCTGCTTCGCGGGTAAACCCGCTCCTACAGGTGGGTGCTGCCCTGAAGACGGCAAGGCCCCTGTAGGAGCGGGTTCACCcgc < 2:387123/150‑1 (MQ=38) gCGATTCCCCTGGGGGTGCAGAAGCTGTTGTGCTGATAGGAGAGCGAGTCGGCTGTTTCGCGGGTGAACCCGCTCCTACAGGTGGGTGCTGCCCTCAAGACGGCAAGGTCCCTGTAGGAGCGGGTTCACCCGCGAAGCAGGCAGCTCGGt < 2:163103/150‑1 (MQ=255) aTTACCCTGGGGGTGCAGAAGCTGTTGGGCTGACTGGAGACCGAGTCGGCTGCTTCGCGGGTAAACCCGCTCCTACAGGTGGGTGCTGCCCTGAAGACGGCAAGGCCCCTGTAGGAGCggg > 2:161134/1‑121 (MQ=255) aTTACCCTGGGGGTGCAGAAGCTGTTGGGCTGACTGGAGACCGAGTCGGCTGCTTCGCGGGTAAACCCGCTCCTACAGGTGGGTGCTGCCCTGAAGACGGCAAGGCCCCTGTAGGAGCggg < 1:161134/121‑1 (MQ=255) ttACCCTGGGGGTGCAGAAGCTGTTGGGCTGACTGGAGACCGAGTCGGCTGCTTCGCGGGTAAACCCGCTCCTACAGGTGGGTGCTGCCCTGAAGACGGCAAGGCCCCTGTAGGAGCGGGTTCACCCGCGAAGCAGGCAGCTCGGTCTCa < 2:508081/150‑1 (MQ=255) gAAGCTGTTGGGCTGACTGGAGACCGAGTCGGCTGCTTCGCGGGTAAACCCGCTCCTACAGGTGGGTGCTGCCCTGAAGACGGCAAGGCCCCTGTAGGAGCGGGTTCACCCGCGAAGCAGGCAGCTCGGTCTCAAGGTTTTTCACAGccc > 1:158675/1‑150 (MQ=255) tGGGCTGACTGGAGACCGAGTCGGCTGCTTCGCGGGTAAACCCGCTCCTACAGGTGGGTGCTGCCCTGAAGACGGCAAGGCCCCTGTAGGAGCGGGTTCACCCGCGAAGCAGGCAGCTCGGTCTCAAGGTTTTTCACAGCCCCCCTCGtt < 2:391819/150‑1 (MQ=255) gtcTGACTGGAGACCGAGTCGGCTGCTTCGCGGGTAAACCAGCTCATACCGGCGGGTGCTGCCCTGAAGACGTCAAGGCCACTGTCGAAACGGATTCATTCTTTAAGCAGGCAGCTCGGTCTCAAGGTTTTTCACAGCCCCCCTCGTTga > 1:391819/3‑149 (MQ=255) ttgagaCCGAGCTGCCTGCTTCGCGGGTGAACCCGCTCCTACAGGTGGGCGCTGCCCTGAAGACGGCAAGGCCCCTGTAGGa > 2:191310/3‑82 (MQ=17) ttgagaCCGAGCTGCCTGCTTCGCGGGTGAACCCGCTCCTACAGGTGGGCGCTGCCCTGAAGACGGCAAGGCCCCTGTAGGa > 2:191311/3‑82 (MQ=17) ttgagaCCGAGCTGCCTGCTTCGCGGGTGAACCCGCTCCTACAGGTGGGCGCTGCCCTGAAGACGGCAAGGCCCCTGTAGGa < 1:191311/80‑1 (MQ=18) ttgagaCCGAGCTGCCTGCTTCGCGGGTGAACCCGCTCCTACAGGTGGGCGCTGCCCTGAAGACGGCAAGGCCCCTGTAGGa < 1:191310/80‑1 (MQ=17) ccGAGTCGGCTGCTTCGCGGGTAAACCCGCTCCTACAGGTGGGTGCTGCCCTGAAGACGGCAAGGCCCCTGTAGGAGCGGGTTCACCCGCGAAGCAGGCAGCTCGGTCTCAAGGTTTTTCACAGCCCCCCTCGTTGCCGTCCACATTtct < 2:405229/150‑1 (MQ=255) aGTCGGCTGCTTCGCGGGTAAACCCGCTCCTACAGGTGGGTGCTGCCCTGAAGACGGCAAGGCCCCTGTAGGAGCGGGTTCACCCGCGAAGCAGGCAGCTCGGTCTCAAGGTTTTTCACAGCCCCCCTCGTTg < 1:76112/133‑1 (MQ=255) aGTCGGCTGCTTCGCGGGTAAACCCGCTCCTACAGGTGGGTGCTGCCCTGAAGACGGCAAGGCCCCTGTAGGAGCGGGTTCACCCGCGAAGCAGGCAGCTCGGTCTCAAGGTTTTTCACAGCCCCCCTCGTTg > 2:76112/1‑133 (MQ=255) ttCGCGGGTAAACCCGCTCCTACAGGTGGGTGCTGCCCTGAAGACGGCAAGGCCCCTGTAGGAGCGGGTTCACCCGCGAAGCAGGCAGCTCGGt > 1:502473/1‑94 (MQ=255) ttCGCGGGTAAACCCGCTCCTACAGGTGGGTGCTGCCCTGAAGACGGCAAGGCCCCTGTAGGAGCGGGTTCACCCGCGAAGCAGGCAGCTCGGt < 2:502473/94‑1 (MQ=255) | TCAGCCAGATTCGCCAGGCTGGGCCACGGGCGCTGGCCACCGGTGCGATCCTCAACCTGTGGCTGGTTGGCGGGGGGCTGGCGATTACCCTGGGGGTGCAGAAGCTGTTGGGCTGACTGGAGACCGAGTCGGCTGCTTCGCGGGTAAACCCGCTCCTACAGGTGGGTGCTGCCCTGAAGACGGCAAGGCCCCTGTAGGAGCGGGTTCACCCGCGAAGCAGGCAGCTCGGTCTCAAGGTTTTTCACAGCCCCCCTCGTTGCCGTCCACATTTCT > NC_002947/4158981‑4159253 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |