| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 4,159,187 | C→T | 14.3% | intergenic (+91/‑118) | PP_3661 → / → PP_3662 | membrane protein/hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 4,159,187 | 0 | C | T | 14.3% | 63.2 / 4.0 | 28 | intergenic (+91/‑118) | PP_3661/PP_3662 | membrane protein/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base C (12/12); new base T (2/2); total (14/14) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.43e-01 | |||||||||||
GCTGGTTGGCGGGGGGCTGGCGATTACCCTGGGGGTGCAGAAGCTGTTGGGCTGACTGGAGACCGAGTCGGCTGCTTCGCGGGTAAACCCGCTCCTACAGGTGGGTGCTGCCCTGAAGACGGCAAGGCCCCTGTAGGAGCGGGTTCACCCGCGAAGCAGGCAGCTCGGTCTCAAGGTTTTTCACAGCCCCCCTCGTTGCCGTCCACATTTCTCGGTATGGTGGTGTCAAAGAAGGATGTTTTACCCGCACAGGAAAGACTTAGATGCCTCAACGCCAT > NC_002947/4159042‑4159319 | gCTGGTTGGCGGGGGGCTGGCGATTACCCTGGGGGTGCAGAAGCTGTTGGGCTGACTGGAGACCGAGTCGGCTGCTTCGCGGGTAAACCCGCTCCTACAGGTGGGTGCTGCCCTGAAGACGGCAAGGCCCCTGTAGGAGCGGGTTCAccc > 2:330951/1‑150 (MQ=255) tGGTTGGCGGGGGGCTGGAGATTAACATCGGGGTGCAGAAGCTGTTTTGCTGACTGGAGACCGAGTCGGCTGCTTCGCGGGTAAACCCGCTCCTACAGGTGGGTGCTGCCCTGAAGACGGCAAGGCCCCTGTAGGAGCGGGTTCACCcgc < 2:387123/150‑1 (MQ=38) gCGATTCCCCTGGGGGTGCAGAAGCTGTTGTGCTGATAGGAGAGCGAGTCGGCTGTTTCGCGGGTGAACCCGCTCCTACAGGTGGGTGCTGCCCTCAAGACGGCAAGGTCCCTGTAGGAGCGGGTTCACCCGCGAAGCAGGCAGCTCGGt < 2:163103/150‑1 (MQ=255) ttACCCTGGGGGTGCAGAAGCTGTTGGGCTGACTGGAGACCGAGTCGGCTGCTTCGCGGGTAAACCCGCTCCTACAGGTGGGTGCTGCCCTGAAGACGGCAAGGCCCCTGTAGGAGCGGGTTCACCCGCGAAGCAGGCAGCTCGGTCTCa < 2:508081/150‑1 (MQ=255) gAAGCTGTTGGGCTGACTGGAGACCGAGTCGGCTGCTTCGCGGGTAAACCCGCTCCTACAGGTGGGTGCTGCCCTGAAGACGGCAAGGCCCCTGTAGGAGCGGGTTCACCCGCGAAGCAGGCAGCTCGGTCTCAAGGTTTTTCACAGccc > 1:158675/1‑150 (MQ=255) tGGGCTGACTGGAGACCGAGTCGGCTGCTTCGCGGGTAAACCCGCTCCTACAGGTGGGTGCTGCCCTGAAGACGGCAAGGCCCCTGTAGGAGCGGGTTCACCCGCGAAGCAGGCAGCTCGGTCTCAAGGTTTTTCACAGCCCCCCTCGtt < 2:391819/150‑1 (MQ=255) gtcTGACTGGAGACCGAGTCGGCTGCTTCGCGGGTAAACCAGCTCATACCGGCGGGTGCTGCCCTGAAGACGTCAAGGCCACTGTCGAAACGGATTCATTCTTTAAGCAGGCAGCTCGGTCTCAAGGTTTTTCACAGCCCCCCTCGTTga > 1:391819/3‑149 (MQ=255) ccGAGTCGGCTGCTTCGCGGGTAAACCCGCTCCTACAGGTGGGTGCTGCCCTGAAGACGGCAAGGCCCCTGTAGGAGCGGGTTCACCCGCGAAGCAGGCAGCTCGGTCTCAAGGTTTTTCACAGCCCCCCTCGTTGCCGTCCACATTtct < 2:405229/150‑1 (MQ=255) aGTCGGCTGCTTCGCGGGTAAACCCGCTCCTACAGGTGGGTGCTGCCCTGAAGACGGCAAGGCCCCTGTAGGAGCGGGTTCACCCGCGAAGCAGGCAGCTCGGTCTCAAGGTTTTTCACAGCCCCCCTCGTTg < 1:76112/133‑1 (MQ=255) aGTCGGCTGCTTCGCGGGTAAACCCGCTCCTACAGGTGGGTGCTGCCCTGAAGACGGCAAGGCCCCTGTAGGAGCGGGTTCACCCGCGAAGCAGGCAGCTCGGTCTCAAGGTTTTTCACAGCCCCCCTCGTTg > 2:76112/1‑133 (MQ=255) ttCGCGGGTAAACCCGCTCCTACAGGTGGGTGCTGCCCTGAAGACGGCAAGGCCCCTGTAGGAGCGGGTTCACCCGCGAAGCAGGCAGCTCGGt > 1:502473/1‑94 (MQ=255) ttCGCGGGTAAACCCGCTCCTACAGGTGGGTGCTGCCCTGAAGACGGCAAGGCCCCTGTAGGAGCGGGTTCACCCGCGAAGCAGGCAGCTCGGt < 2:502473/94‑1 (MQ=255) ccGCTCCTACAGGTGGGTGCTGCCCTGAAGACGGCAAGGCCCCTGTAGGAGCGGGTTCACCCGCGAAGCAGGCAGCTCGGTCTCAAGGTTTTTCACAGCCCCCCTCGTTGCCGTCCACATTTCTCGGTATGGTGGTGTCAaagaa < 1:54721/145‑1 (MQ=255) ccGCTCCTACAGGTGGGTGCTGCCCTGAAGACGGCAAGGCCCCTGTAGGAGCGGGTTCACCCGCGAAGCAGGCAGCTCGGTCTCAAGGTTTTTCACAGCCCCCCTCGTTGCCGTCCACATTTCTCGGTATGGTGGTGTCAaagaa > 2:54721/1‑145 (MQ=255) cTACAGGTGGGTGCTGCCCTGAAGACGGCAAGGCCCCTGTAGGAGCGGGTTCACCCGCGAAGCAGGCAGCTCGGTCTCAAGGTTTTTCACAGCCCCCCTCGTTGCCGTCCACATTTCTCGGTAtgg < 2:491035/126‑1 (MQ=255) cTACAGGTGGGTGCTGCCCTGAAGACGGCAAGGCCCCTGTAGGAGCGGGTTCACCCGCGAAGCAGGCAGCTCGGTCTCAAGGTTTTTCACAGCCCCCCTCGTTGCCGTCCACATTTCTCGGTAtgg > 1:491035/1‑126 (MQ=255) gtgggtgCTGCCCTGAAGACGGCAAGGCCCCTGTAGGAGCGGGTTTACCCGCGAAGCAGGCAGCTCGGTCTCAAGGTTTTTCACAGCCCCCCTCGTTGCCGTCCACATTTCTCGGTATGGTGGTGTCAAAGAAGGATGTTTTACCCTcac > 1:21518/1‑150 (MQ=255) gtgggtgCTGCCCTGAAGACGGCAAGGCCCCTGTAGGAGCGGGTTTACCCGCGAAGCAGGCAGCTCGGTCTCAAGGTTTTTCACAGCCCCCCTCGTTGCCGTCCACATTTCTCGGTATGGTGGTGTCAAAGAAGGATGTTTTACCCGcac > 1:21566/1‑150 (MQ=255) gggtgCTGCCCTGAAGACGGCAAGGCCCCTGTAGGAGCGGGTTCACCCGCGAAGCAGGCAGCTCGGTCTCAAGGTTTTTCACAGCCCCCCTCGTTGCCGTCCACATTTCTCGGTATGGTGGTGTCAAAGAAGGATGTTTTACCCGCACAg > 2:376482/1‑150 (MQ=255) gggtgCTGCCCTGAAGACGGCAAGGCCCCTGTAGGAGCGGGTTCACCCGCGAAGCAGGCAGCTCGGTCTCAAGGTTTTTCACAGCCCCCCTCGTTCCCGTCCACATTTCTCGGTATGGTGGTGTCAAAGAAGGATGTTTTACCCGCACAg < 1:239984/150‑1 (MQ=255) tgcCCTGAAGACGGCAAGGCCCCTGTAGGAGCGGGTTCACCCGCGAAGCAGGCAGCTCGGTCTCAAGGTTTTTCACAGCCCCCCTCGTTGCCGTCCACATTTCTCGGTATGGTGGTGTCAAAGAAGGATGTTTTACCCGCACAGGAAAGa > 1:126715/1‑150 (MQ=255) tgcCCTGAAGACGGCAAGGCCCCTGTAGGAGCGGGTTCACCCGCGAAGCAGGCAGCTCGGTCTCAAGGTTTTTCACAGCCCCCCTCGTTGCCGTCCACATTTCTCGGTATGGTGGTGTCAAAGAAGGATGTTTTACCCGCACAGGAAAGa < 1:330951/150‑1 (MQ=255) gcCCTGAAGACGGCAAGGCCCCTGTAGGAGCGGGTTCACCCGCGAAGCAGGCAGCTCGGTCTCAAGGTTTTTCACAGCCCCCCTCGTTGCCGTCCACATTTCTCGGTATGGTGGTGTCAAAGAAGGATGTTTTACCCGCACAGGAAAGAc < 1:376482/150‑1 (MQ=255) ggCAAGGCCCCTGTAGGAGCGGGTTCACCCGCGAAGCAGGCAGCTCGGTCTCAAGGTTTTTCACAGCCCCCCTCGTTGCCGTCCACATTTCTCGGTATGGTGGTGTCAAAGAAGGATGTTTTACCCGCACAGGAAAGACTTAGATGCCTc > 2:487520/1‑150 (MQ=255) gCAAGGCCCCTGTAGGAGCGGGTTCACCCGCGAAGCAGGCAGCTCGGTCTCAAGGTTTTTCACAGCCCCCCTCGTTGCCGTCCACATTTCTCGGTATGGTGGTGTCAAAGAAGGATGTTTTACCCGCACAGGAAAGACTTAGATGCCTCa > 1:7860/1‑150 (MQ=255) aaGGCCCCTGTAGGAGCGGGTTTACCCGCGAAGCAGGCAGCTCGGTCTCAAGGTTTTTCACAGCCCCCCTCGTTGCCGTCCACATTTCTCGGTATGGTGGTGTCAAAGAAGGATGTTTTACCCGCACAGGAAAGACTTAGATGCCTCAAc < 2:21566/150‑1 (MQ=255) aaGGCCCCTGTAGGAGCGGGTTTACCCGCGAAGCAGGCAGCTCGGTCTCAAGGTTTTTCACAGCCCCCCTCGTTGCCGTCCACATTTCTCGGTATGGTGGTGTCAAAGAAGGATGTTTTACCCGCACAGGAAAGACTTAGATGCCTCAAc < 2:21518/150‑1 (MQ=255) cccTGTAGGAGCGGGTTCACCCGCGAAGCAGGCAGCTCGGTCTCAAGGTTTTTCACAGCCCCCCTCGTTGCCGTCCACATTTCTCGGTATGGTGGTGTCAAAGAAGGATGTTTTACCCGCACAGGAAAGACTTAGATGCCTCAACGCCAt > 2:117163/1‑150 (MQ=255) | GCTGGTTGGCGGGGGGCTGGCGATTACCCTGGGGGTGCAGAAGCTGTTGGGCTGACTGGAGACCGAGTCGGCTGCTTCGCGGGTAAACCCGCTCCTACAGGTGGGTGCTGCCCTGAAGACGGCAAGGCCCCTGTAGGAGCGGGTTCACCCGCGAAGCAGGCAGCTCGGTCTCAAGGTTTTTCACAGCCCCCCTCGTTGCCGTCCACATTTCTCGGTATGGTGGTGTCAAAGAAGGATGTTTTACCCGCACAGGAAAGACTTAGATGCCTCAACGCCAT > NC_002947/4159042‑4159319 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |