Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,272,817 G→A 12.5% V23I (GTC→ATC)  PP_3743 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,272,8170GA12.5% 70.9 / 4.2 32V23I (GTC→ATC) PP_3743hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (14/14);  new base A (2/2);  total (16/16)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.76e-01

AACTACCCCAACGCCCCCGCCGTCTTAGATACTGAGTGGTTTATTTCCCTGTGCTTCACGCTACTGGAGAACACGACATGCGCCACCTACTGCTCGCCCCTGCGCTGCTGCTCCTGATGCCTGCCGGTGCTGCCCTGGCCCGCGTCGATGCGGGCGACGTCGCCACCTCGGCCGGTATTTCCGCTTCGCTGTATTCGACCTTCAAGGACGACAAACGGATCATCCCTGCCCGTGACGAACTTTCAGCGTTCGTCGCCAGCGGCGGTACCATCCGCGGCGCCTATGTGGA  >  NC_002947/4272674‑4272962
                                                                                                                                               |                                                                                                                                                 
aaCTACCCCAACGCCCCCGCCGTCTTAGATACTGAGTGGTTTATTTCCCTGTGCTTCACGCTACTGGAGAACACGACATGCGCCACCTACTGCTCGCCCCTGCGCTGCTGCTCCTGATGCCTGCCGGTGCTGCCCTGGCCCGCGTCGATg                                                                                                                                             >  1:453220/1‑150 (MQ=255)
 aCTACCCCAACGCCCCCGCCGTCTTAGATACTGAGTGGTTTATTTCCCTGTGCTTCACGCTACTGGAGAACACGACATGCGCCACCTACTGCTCGCCCCTGCGCTGCTGCTCCTGATGCCTGCCGGTGCTGCCCTGGCCCGCGTCGATGc                                                                                                                                            <  2:121621/150‑1 (MQ=255)
   tACCCCAACGCCCCCGCCGTCTTAGATACTGAGTGGTTTATTTCCCTGTGCTTCACGCTACTGGAGAACACGACATGCGCCACCTACTGCTCGCCCCTGCGCTGCTGCTCCTGATGCCTGCCGGTGCTGCCCTGGCCCGCATCGACGCgg                                                                                                                                          >  2:460531/1‑150 (MQ=255)
        cAACGCCCCCGCCGTATTAGATAGTGAGTGGTTTATTTCACTGTGCTTCACGCCACTGGAGAAAACGACATGCGCGACCTAACACTCAGCCCGGCGGTGCTGCTCCGGATGCCTGCCGGTGCTGCCTTGGCCGGGGTCGATGCgtcgccc                                                                                                                                     >  2:236357/1‑144 (MQ=255)
         aaCGCCCCCGCCGTCTTAGATACTGAGTGGTTTATTTCCCTGTGCTTCACGCTACTGGAGAACACGACATGCGCCACCTACTGCTCGCCCCTGCGCTGCTGCTCCTGATGCCTGCCGGTGCTGCCCTGGCCCGCATCGACGCGGGccagg                                                                                                                                    <  1:460531/150‑5 (MQ=255)
           cGCCCCCGCCGTCTTAGATACTGAGTGGTTTATTTCCCTGTGCTTCACGCTACTGGAGAACACGACATGCGCCACCTACTGCTCGCCCCTGCGCTGCTGCTCCTGATGCCTGCCGGTGCTGCCCTGGCCCGCGTCGATGCGGGCGACGTc                                                                                                                                  <  1:345421/150‑1 (MQ=255)
              ccccGCCGTCTTAGATACTGAGTGGTTTATTTCCCTGTGCTTCACGCTACTGGAGAACACGACATGCGCCACCTACTGCTCGCCCCTGCGCTGCTCCTCCTGATGCCTGCCGGTGCTGCCCTGGCCCGCGTCGATGCGGGCGACGTCGcc                                                                                                                               >  2:167121/1‑150 (MQ=255)
                ccgccgTCTTAGATACTGAGTGGTTTATTTCCCTGTGCTTCACGCTACTGGAGAACACGACATGCGCCACCTACTGCTCGCCCCTGCGCTGCTGCTCCTGATGCCTGCCGGTGCTGCCCTGGCCCGCGTCGATGCGGGCGACGTCGCCAc                                                                                                                             >  2:343745/1‑150 (MQ=255)
                ccgccgTCTTAGATACTGAGTGGTTTATTTCCCTGTGCTTCACGCTACTGGAGAACACGACATGCGCCACCTACTGCTCGCCCCTGCGCTGCTGCTCCTGATGCCTGCCGGTGCTGCCCTGGCCCGCGTCGATGCGGGCGACGTCGCCAc                                                                                                                             >  1:83406/1‑150 (MQ=255)
                         tAGATACTGAGTGGTTTATTTCCCTGTGCTTCACGCTACTGGAGAACACGACATGCGCCACCTACTGCTCGCCCCTGCGCTGCTGCTCCTGATGCCTGCCGGTGCTGCCCTGGCCCGCGTCGATGCGGGCGACGTCGCCACCTCGGCCgg                                                                                                                    >  1:348266/1‑150 (MQ=255)
                              aCTGAGTGGTTTATTTCCCTGTGCTTCACGCTACTGGAGAACACGACATGCGCCACCTACTGCTCGCCCCTGCGCTGCTGCTCCTGATGCCTGCCGGTGCTGCCCTGGCCCGCGTCGATGCGGGCGACGTCGCCACCTCGGCCGGTAttt                                                                                                               <  2:348266/150‑1 (MQ=255)
                                tGAGTGGTTTATTTCCCTGTGCTTCACGCTACTGGAGAACACGACATGCGCCACCTACTGCTCGCCCCTGCGCTGCTGCTCCTGATGCCTGCCGGTGCTGCCCTGGCCCGCGTCGATGCGGGCGACGTCGCCACCTCGGCCGGTATTTcc                                                                                                             >  2:301097/1‑150 (MQ=255)
                                       tttATTTCCCTGTGCTTCACGCTACTGGAGAACACGACATGCGCCACCTACTGCTCGCCCCTGCGCTGCTGCTCCTGATGCCTGCCGGTGCTGCCCTGGCCCGCGTCGATGCGGGCGACGTCGCCACCTCGGCCGGTATTTCCGCTTCGc                                                                                                      <  1:487879/150‑1 (MQ=255)
                                            ttCCCTGTGCTTCACGCTCCTGGAGAACACGACATGCGCCCTCTATTGCTCGCCCCTGCGCTGCTGCTCCTGATGCCTGCCGGTGCTGCCCTGGCCCGCGTCGATGCGGGCGACGTCGCCACCTCGGCCGGTATTTCCGCTTCGCTGTAt                                                                                                 <  2:83406/150‑1 (MQ=255)
                                                       tCACGCTACTGGAGAACACGACATGCGCCACCTACTGCTCGCCCCTGCGCTGCTGCTCCTGATGCCTGCCGGTGCTGCCCTGGCCCGCGTCGATGCGGGCGACGTCGCCACCTCGGCCGGTATTTCCGCTTCGCTGTATTCGACCTTCaa                                                                                      <  2:453220/150‑1 (MQ=255)
                                                        cACGCTACTGGAGAACACGACATGCGCCACCTACTGCTCGCCCCTGCGCTGCTGCTCCTGATGCCTGCCGGTGCTGCCCTGGCCCGCGTCGATGCGAGCGACGTCGCCACCTCGGCCGGTATTTCCGCTTCGCTGTATTCGACCTTCAAg                                                                                     >  1:340970/1‑150 (MQ=255)
                                                                  gagaACACGACATGCGCCACCTACTGCTCGCCCCTGCGCTGCTGCTCCTGATGCCTGCCGGTGCTGCCCTGGCCCGCGTCGATGCGGGCGACGTCGCCACCTCGGc                                                                                                                       <  1:96622/106‑1 (MQ=255)
                                                                  gagaACACGACATGCGCCACCTACTGCTCGCCCCTGCGCTGCTGCTCCTGATGCCTGCCGGTGCTGCCCTGGCCCGCGTCGATGCGGGCGACGTCGCCACCTCGGc                                                                                                                       >  2:96622/1‑106 (MQ=255)
                                                                  gagaACACGACATGCGCCACCTACTGCTCGCCCCTGCGCTGCCGCTCCTGATGCCTGCCGGTGCTGCCCTGGCCCGCGTCGATGCGGGCGACGTCGCCACCTCGGCCGGTATTTCCGCTTCGCTGTATTCGACCTTCAAGGACGACAAAc                                                                           <  1:236357/150‑1 (MQ=255)
                                                                            cATGCGCCACCTACTGCTCGCCCCTGCGCTGCTGCTCCTGATGCCTGCCGGTGCTGCCCTGGCCCGCGTCGATGCGGGCGACGTCGCCACCTCGGCCGGTATTTCCGCTTCGCTGTATTCGACCTTCAAGGACGACAAACGGa                                                                        <  1:508192/143‑1 (MQ=255)
                                                                            cATGCGCCACCTACTGCTCGCCCCTGCGCTGCTGCTCCTGATGCCTGCCGGTGCTGCCCTGGCCCGCGTCGATGCGGGCGACGTCGCCACCTCGGCCGGTATTTCCGCTTCGCTGTATTCGACCTTCAAGGACGACAAACGGa                                                                        >  2:508192/1‑143 (MQ=255)
                                                                                   cACCTACTGCTCGCCCCTGCGCTGCTGCTCCTGATGCCTGCCGGTGCTGCCCTGGCCCGCGTCGATGCGGGCGACGTCGCCACCTCGGCCGGTATTTCCGCTTCGCTGTATTCGACCTTCAAGGACGACAAACGGATCATCCCTGCCCGt                                                          >  1:307823/1‑150 (MQ=255)
                                                                                         cTGCTCGCCCCTGCGCTGCTGCTCCTGATGCCTGCCGGTGCTGCCCTGGCCCGCGTCGATGCGGGCGACGTCGCCCCCTCGGCCGGTATTTCCGCTTCGCTGTATTCGACCTTCAAGGACGACAAACGGATCATCCCTGCCCGTGACGaa                                                    <  2:151914/150‑1 (MQ=255)
                                                                                          tGCTCGCCCCTGCGCTGCTGCTCCTGATGCCTGCCGGTGCTGCCCTGGCCCGCGTCGATGCGGGCGACGTCGCCACCTCGGCCGGTATTTCCGCTTCGCTGTATTCGACCTTCAAGGACGACAAACGGATCATCCCTGCCCGTGACGAAc                                                   >  2:226323/1‑150 (MQ=255)
                                                                                                 cccTGCGCTGCTGCTCCTCATGCCTGCCGGTGCTGCCCTGGCCCGCGTCGATGCGGGCGACGTCGCCACCTCGGCCGGTATTTCCGCTTCGCTGTATTCGACCTTCAAGGACGCCAAACGGATCATCCCTGCCCGTGACGAACTTTCAGc                                            <  1:270961/150‑1 (MQ=255)
                                                                                                     gcgcTGCTGCTCCTGATGCCTGCCGGTGCTGCCCTGGCCCGCGTCGATGCGGGCGACGTCGCCACCTCGGCCGGTATTTCCGCTTCGCTGTATTCGACCTTCAAGGACGACAAACGGATCATCCCTGCCCGTGACGAACTTTCAGCGTtc                                        <  1:343745/150‑1 (MQ=255)
                                                                                                                      gcctgccGGTGCTGCCCTGGCCCGCGTCGATGCGGGCGACGTCGCCACCTCGGCCGGTATTTCCGCTTCGCTGTATTCGACCTTCAAGGACGACAAACGGATCATCCCTGCCCGTGACGAACTTTCAGCGTTCGTCGCTACCGGCGGTAc                       >  2:498289/1‑150 (MQ=255)
                                                                                                                                      gtcgCCCGCATCGACGCGGGCGACGTCGCCACCTCGGCCGGTATTTCCGCTTCGCTGTATTCGACCTTCAAGGACGACAAACGGATCATCCCTGCCCGTGACGAACTTTCa                                              <  1:497638/108‑1 (MQ=255)
                                                                                                                                      gtcgCCCGCATCGACGCGGGCGACGTCGCCACCTCGGCCGGTATTTCCGCTTCGCTGTATTCGACCTTCAAGGACGACAAACGGATCATCCCTGCCCGTGACGAACTTTCa                                              >  2:497638/4‑111 (MQ=255)
                                                                                                                                         gCCCGCGTCGATGCGGGCGACGTCGCCACCTCGGCCGGTATTTCCGCTTCGCTGTATTCGACCTTCAAGGACGACAAACGGATCATCCCTGCCCGTGACGAACTTTCAGCGTTCGTCGCCAGCGGCGGTACCATCCGCGGCGCCTAtgtg    <  2:200372/150‑1 (MQ=255)
                                                                                                                                           ccGCGTCGATGCGGGCGACGTCGCCACCTCGGCCGGTATTTCCGCTTCGCTGTATTCGACCTTCAAGGACGACAAACGGATCATCCCTGCCCGTGACGAACTTTCAGCGTTCGTCGCCAGCGGCGGTACCATCCGCGGCGCCTATGTGGa  >  2:48795/1‑150 (MQ=255)
                                                                                                                                           ccGCGTCGATGCGGGCGACGTCGCCACCTCGGCCGGTATTTCCGCTTCGCTGTATTCGACCTTCAAGGACGACAAACGGATCATCCCTGCCCGTGACGAACTTTCAGCGTTCGTCGCCAGCGGCGGTACCATCCGCGGCGCCTATGTGGa  <  1:226323/150‑1 (MQ=255)
                                                                                                                                               gTCGATGCGGGCGACGTCGCCACCTCGGCCGGTATTTCCGCTTCGCTGTATTCGACCTTCAAGGACGACAAACGGATCATCCCTGCCCGTGACGAACTTTCAGCGTTCGTCGCCAGCGGCGGTACCATCCGCGGCGCCTAt       >  1:41326/1‑141 (MQ=255)
                                                                                                                                               gTCGATGCGGGCGACGTCGCCACCTCGGCCGGTATTTCCGCTTCGCTGTATTCGACCTTCAAGGACGACAAACGGATCATCCCTGCCCGTGACGAACTTTCAGCGTTCGTCGCCAGCGGCGGTACCATCCGCGGCGCCTAt       <  2:41326/141‑1 (MQ=255)
                                                                                                                                               |                                                                                                                                                 
AACTACCCCAACGCCCCCGCCGTCTTAGATACTGAGTGGTTTATTTCCCTGTGCTTCACGCTACTGGAGAACACGACATGCGCCACCTACTGCTCGCCCCTGCGCTGCTGCTCCTGATGCCTGCCGGTGCTGCCCTGGCCCGCGTCGATGCGGGCGACGTCGCCACCTCGGCCGGTATTTCCGCTTCGCTGTATTCGACCTTCAAGGACGACAAACGGATCATCCCTGCCCGTGACGAACTTTCAGCGTTCGTCGCCAGCGGCGGTACCATCCGCGGCGCCTATGTGGA  >  NC_002947/4272674‑4272962

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: