| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 471,070 | G→A | 10.8% | intergenic (‑111/+17) | PP_0386 ← / ← rpoD | sensory box protein/RNA polymerase sigma 70 factor |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 471,070 | 0 | G | A | 10.8% | 93.3 / 3.8 | 37 | intergenic (‑111/+17) | PP_0386/rpoD | sensory box protein/RNA polymerase sigma 70 factor |
| Reads supporting (aligned to +/- strand): ref base G (18/15); new base A (2/2); total (20/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.53e-01 | |||||||||||
TCAGCAACAGAATGGCCGGCTTCAAGGACATGGCAGCCTCGCACTCAGGTGGTCTGAAGTCGAGTGTAGACGGGCATTGCCGCAGGTGTGCAACGGAAGGCGGGCTTTATGTCACGGGCCACTAAAGAAAAACCCCGGCCTGGGCCGGGGTTTTGTCATCACTCGTCGAGGAAGGAGCGCAGGTGCTCGCTGCGAGTCGGGTGGCGCAGCTTGCGCAACGCCTTCGCTTCGATCTGACGGATCCGCTCACGCGTTACGTCAAACTGCTTGCCCACCTCTTCGAGGGTG > NC_002947/470929‑471216 | tCAGCAACAGAATGGCCGGCTTCAAGGACATGGCAGCCTCGCAATCAGGTGGTCTGAAGTCGAGTGTAGACGGGCATTGCCGCAGGTGTGCAACGGAAGGCGGGCTTTATGTCACGGGCCACTAAAGAAAAACCCCGGCCCAGGCCgggg < 2:185488/150‑1 (MQ=255) gCAACAGAATGGCCGGCTTCAAGGACATGGCAGCCTCGCACTCAGGTGGTCTGAAGTCGAGTGTAGACGGGCATTGCCGCAGGTGTGCAACGGAAGGCGGGCTTTATGTCACGGGCCACTAAAGAAAAACCCCGGCCTGGGCCGGGGttt > 1:349217/1‑150 (MQ=255) gCAACAGAATGGCCGGCTTCAAGGACATGGCAGCCTCGCACTCAGGTGGTCTGAAGTCGAGTGTAGACGGGCATTGCCGCAGGTGTGCAACGGAAGGCGGGCTTTATGTCACGGGCCACTAAAGAAAAACCCCGGCCCAGGCCGGGGttt < 1:107264/150‑1 (MQ=255) ccGGCTTCAAGGACATGGCAGCCTCGCACTCAGGTGGTCTGAAGTCGAGTGTAGACGGGCATTGCCGCAGGTGTGCAACGGAAGGCGAGCTTTATGTCACGGGCCACTAAAGAAAAACCCCGGCCTGGGCCGGGGTTTTGTCATCACtcg > 1:392029/1‑150 (MQ=255) gACATGGCAGCCTCGCACTCAGGTGGTCTGAAGTCGAGTGTAGACGGGCATTGCCGCAGGTGTGCAACGGAAGGCGGGCTTTATGTCACGGGCCACTAAAGAAAAACCCCGGCCTGGGCCGGGGTTTTGTCATCACTCGTCGaggaagga < 2:380178/150‑1 (MQ=255) gACATGGCAGCCTCGCACTCAGGTGGTCTGAAGTCGAGTGTAGACGGGCATTGCCGCAGGTGTGCAACGGAAGGCGGGCTTTATGTCACGGGCCACTAAAGAAAAACCCCGGCCTGGGCCGGGGTTTTGTCATCACTCGTCGaggaagga > 2:301806/1‑150 (MQ=255) gCAGCCTCGCACTCAGGTGGTCTGAAGTCGAGTGTAGACGGGCATTGCCGCAGGTGTGCAACGGAAGGCGGGCTTTATGTCACGGGCCACTAAAGAAAAACCCCGGCCTGGGCCGGGGTTTTGTCATCACTCGTCGAGGAAGGAGCGCAg < 2:244661/150‑1 (MQ=255) tCGCACTCAGGTGGTCTGAAGTCGAGTGTAGACGGGCATTGCCGCAGGTGTGCAACGGAAGGCGGGCTTTATGTCACGGGCCACTAAAGAAAAACCCCGGCCTGGGCCGGGGTTTTGTCATCACTCGTCGAGGAAGGAGCGCAGGTGCTc > 1:285102/1‑150 (MQ=255) tCGCACTCAGGTGGTCTGAAGTCGAGTGTAGACGGCCATTGCCGCCGGTGTGCAACAGAAGGCGGGCTTTATTTCACGGGCCACTAAAGAAAAACCCCGGCCTGGGCGGGGGTTTTTTCATCACTCGTCCAGCAAGGAGCGCAGGTGCTc > 2:192491/1‑150 (MQ=255) cGCACTCAGGTGGTCTGAAGTCGAGTGTAGACGGGCATTGCCGCAGGTGTGCAACGGAAGGCGGGCTTTATGTCACGGGCCACTAAAGAAAAACCCCGGCCTGGGCCGGGGTTTTGTCATCACTCGTCGAGGAAGGAGCGCAGGTGCTCg < 1:301806/150‑1 (MQ=255) aCTCAGGTGGTCTGAAGTCGAGTGTAGACGGGCATTGCCGCAGGTGTGCAACGGAAGGCGGGCTTTATGTCACGGGCCACTAAAGAAAAACCCCGGCCTGGGCCGGGGTTTTGTCATCACTCGTCGAGGAAGGAGCGCAGGTGCTCGCTg < 2:350521/150‑1 (MQ=255) cTCAGGTGGTCTGAAGTCGAGTGTAGACGGGCATTGCCGCATGTGTGCAACGGAAGGCGGGCTTTATGTCACGGGCCACTAAAGAAAAACCCCGGCCTGGGCCGGGGTTTTGTCATCACTCGTCGAGGAAGGAGCGCAGGTGCTCGCTGc < 1:338186/150‑1 (MQ=255) tCAGGTGGTCTGAAGTCGAGTGTAGACGGGCATTGCCGCAGGTGTGCAACGGAAGGCGGGCTTTATGTCACGGGCCACTAAAGAAAAACCCCGGCCTGGGCCgggg < 2:469114/106‑1 (MQ=255) tCAGGTGGTCTGAAGTCGAGTGTAGACGGGCATTGCCGCAGGTGTGCAACGGAAGGCGGGCTTTATGTCACGGGCCACTAAAGAAAAACCCCGGCCTGGGCCgggg > 1:469114/1‑106 (MQ=255) aGGTGGTCTGAAGTCGAGTGTAGACGGGCATTGCCGCAGGTGTGCAACGGAAGGCGGGCTTTATGTCACGGGCCACTAAAGAAAAACCCCGGCCTGGGCCGGGGTTTTGTCATCACTCGTCGAGGAAGGAGCGCAGGTGCTCGCTGCGAg > 1:181140/1‑150 (MQ=255) tCTGAAGTCGAGTGTAGACGGGCATTGCCGCAGGTGTGCAACGGAAGGCGGGCTTTATGTCACGGGCCACTAAAGAAAAACCCCGGCCTGGGCCGGGGTTTTGTCATCACTCGTCGAGGAATGAGCGCAGGTGCTCGCTGCGAGTCGGGt > 2:322306/1‑150 (MQ=255) cTGAAGTCGAGTGTAGACGGGCATTGCCGCAGGTGTGCAACGGAAGGCGGGCTTTATGTCACGGGCCACTAAAGAAAAACCCCGGCCTGGGCCGGGGTTTTGtc < 2:486959/104‑1 (MQ=255) cTGAAGTCGAGTGTAGACGGGCATTGCCGCAGGTGTGCAACGGAAGGCGGGCTTTATGTCACGGGCCACTAAAGAAAAACCCCGGCCTGGGCCGGGGTTTTGtc > 1:486959/1‑104 (MQ=255) cTGAAGTCGAGTGTAGACGGGCATTGCCGCAGGTGTGCAACGGAAGGCGGGCTTTATGTCACGGGCCACTAAAGAAAAACCCCGGCCTGGGCCGGGGTTTTGTCATCACTCGTCGAGGAAGGAGCGCAGGTGCTCGCTGCGAGTCGGGTg < 2:276947/150‑1 (MQ=255) cTGAAGTCGAGTGTAGACGGGCATTGCCGCAGGTGTGCAACGGAAGGCGGGCTTTATGTCACGGGCCACTAAAGAAAAACCCCGGCCTGGGCCGGGGTTTTGTCATCACTCGTCGAGGAAGGAGCGCAGGTGCTCGCTGCGAGTCGGGTg < 2:285102/150‑1 (MQ=255) gggCATTGCCGCAGGTGTGCAACGGAAGGCGGGCTTTATGTCACGGGCCACTAAAGAAAAACCCCGGCCTGGGCCGGGGTTTTGTCATCACTCGTCGAGGAAGGAGCGCAGGTGCTCGCTGCGAGTCGGGTGGCGCAGCTTGCGCAACGc > 2:397115/1‑150 (MQ=255) aaCGGAAGGCGGGCTTTATGTCACGGGCCACTAAAGAAAAACCCCGGCCTGGGCCGGGGTTTTGTCATCACTCGTCGAGGAAGGAGCGCAGGTGCTCGCTGCGAGTCGGGTGGCGCAGCTTGCGCAACGCCTTCGCTTCGATCTGACGGa > 1:439754/1‑150 (MQ=255) gggCTTTATGTCACGGGCCACTAAAGAAAAACCCCGGCCTGGGCCGGGGTTTTGTCATCACTCGTCGAGGAAGGAGCGCAGGTGCTCGCTGCGAGTCGGGTGGCGCAGCTTGCGCAACGCCTTCGCTTCGATCTGACGGATCCGCTCAcg > 2:466545/1‑150 (MQ=255) gtcTTTATGTCACGGGCCACTAAAGAAAAACCCCGGCCTGGGCCGGGGTTTTGTCATCACTCGTCGAGGAAGGAGCGCAGGTGCTCGCTGCGAGTCGGGTGGCGCAGCTTGCGCAACGCCTTCGCTTCGATCTGACGGATCCGCTCAcgc < 2:369438/148‑1 (MQ=255) tATGTCACGGGCCACTAAAGAAAAACCCCGGCCTGGGCCGGGGTTTTGTCATCACTCGTCGAGGAAGGAGCGCAGGTGCTCGCTGCGAGTCGGGTGGCGCAGCTTGCGCAACGCCTTCGCTTCGATCTGACGGATCCGCTCACGCGTTAc < 2:181140/150‑1 (MQ=255) tCACGGGCCACTAAAGAAAAACCCCGGCCTGGGCCGGGGTTTTGTCATCACTCGTCGAGGAAGGAGCGCAGGTGCTCGCTGCGAGTCGGGTGGCGCAGCTTGCGCAACGCCTTCGCTTCTATCTGACGGATCCGCTCACGCGTTACGTCa > 2:408561/1‑150 (MQ=255) cACTAAAGAAAAACCCCGGCCTGGGCCGGGGTTTTGTCATCACTCGTCGAGGAAGGAGCGCAGGTGCTCGCTGCGAGTCggg < 1:450094/82‑1 (MQ=255) cACTAAAGAAAAACCCCGGCCTGGGCCGGGGTTTTGTCATCACTCGTCGAGGAAGGAGCGCAGGTGCTCGCTGCGAGTCggg > 2:450094/1‑82 (MQ=255) tgatgacaaaaCCCCGGCCCAGGCCGGGGTTTTGTCATCACTCGTCGAGGAAGGAGCGCAGGTGCTCGCTGCGAGTCGGGTGGCGCAGCTTGCGCAACGCCTTCGCTTCGATCTGACGGATCCGCTCACGCGTTACGTCAAACTGCTTGc > 2:182166/8‑150 (MQ=255) cTAAAGAACGACCCCGGCCTGGGCCGGGGTTTTGTCAGCTCCCGTCGAGGAAGGGGCGCAGGTGCTCGCTGAGAGTCGGGTGCCGCACCTTGCGCTACGCATCCGCTTCGCTCTGACGGATCCGCTCACGCGTTACGCCAAACTGcgggc < 2:92788/150‑5 (MQ=255) cTAAAGAAAAACCCCGGCCTGGGCCGGGGTTTTGTCATCACTCGTCGAGGAAGGAg < 1:52032/56‑1 (MQ=255) cTAAAGAAAAACCCCGGCCTGGGCCGGGGTTTTGTCATCACTCGTCGAGGAAGGAg > 2:52032/1‑56 (MQ=255) tAAAGAAAAACCCCGGCCTGGGCCGGGGTTTTGTCATCACTCGTCGAGGAAGGAGCGCAGGTGCTCGCTGCGAGTCGGGTGGCGCAGCTTGCGCAACGCCTTCGCTTCGATCTGACGGATCCGCTCACGCGTTACGTCAAACTGCTTGcc > 1:28080/1‑150 (MQ=255) aaaaCCCCGGCCCAGGCCGGGGTTTTGTCATCACTCGTCGAGGAAGGAGCGCAGGTGCTGGCTGCGAGTCGGGTGGCGCAGCTTGCGCAACGCCTTCGCTTCGATCTGACGGATCCGCTCACGCGTTACATCGAACTGCTTGCCCACctc > 1:26238/1‑150 (MQ=255) aaaCCCCGGCCTGGGCCGGGGTTTTGTCATCACTCGTCGAGGAAGGAGCGCAGGTGCTCGCTGCGAGTCGGGTGGCGCAGCTTGCGCAACGCCTTCGCTTCGATCTGACGGATCCGCTCAcgc > 2:363582/1‑123 (MQ=255) aaaCCCCGGCCTGGGCCGGGGTTTTGTCATCACTCGTCGAGGAAGGAGCGCAGGTGCTCGCTGCGAGTCGGGTGGCGCAGCTTGCGCAACGCCTTCGCTTCGATCTGACGGATCCGCTCAcgc < 1:363582/123‑1 (MQ=255) ccTGGGCCGGGGTTTTGTCATCACTCGTCGAGGACGGAGCGCAGGTGCTCGCTGCGAGTCGGGTGGCGCAGCTTGCGCAACGCCTTCGCTTCGATCTGACGGCTCCGCTCACGCGTTCCGCCAATCTTCTTGCACACCTCTTCGAGGgtg > 2:110210/1‑150 (MQ=255) | TCAGCAACAGAATGGCCGGCTTCAAGGACATGGCAGCCTCGCACTCAGGTGGTCTGAAGTCGAGTGTAGACGGGCATTGCCGCAGGTGTGCAACGGAAGGCGGGCTTTATGTCACGGGCCACTAAAGAAAAACCCCGGCCTGGGCCGGGGTTTTGTCATCACTCGTCGAGGAAGGAGCGCAGGTGCTCGCTGCGAGTCGGGTGGCGCAGCTTGCGCAACGCCTTCGCTTCGATCTGACGGATCCGCTCACGCGTTACGTCAAACTGCTTGCCCACCTCTTCGAGGGTG > NC_002947/470929‑471216 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |