Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	4,512,506	C→A	33.3%	G9V (GGT→GTT)	lolA ←	outer membrane lipoprotein carrier protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	4,512,506	0	C	A	33.3%	14.5 / 8.1	15	G9V (GGT→GTT)	lolA	outer membrane lipoprotein carrier protein
Reads supporting (aligned to +/- strand):  ref base C (5/5);  new base A (2/3);  total (7/8)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.36e-01

TCTGCGACTTTTCCAGCAACTGGGTCAGGCGTTGTACGTCTTGCTCACCGGCATAAGCAGTAACCGAGCCCAGGGTCAGGGCAGAAACCAACAGCATGCGAATCGCGCGCATGGGAATCCTCATTGAGCGTTGAAAAGGCCTGGCGCCACCGTTGGCGCCAGGCAGGGTGTTCATCAGTCGCGCGGGCCGCCCGGGGCTATCACTTCCCGCGAGCCGTTGCTGTTCATCGGGGTGACAACGCCGGCCATCTCCATCGACTCGATCATGCGCGCGGCACGGTTGTAGCCGA  >  NC_002947/4512357‑4512646                                                                                                                                                      |                                                                                                                                             tCTGCGACTTTTCCAGCAACTGGGTCAGGCGTTGTACGTCTTGCTCACCGGCATAAGCAGTAACCGAGCCCAGGGTCAGGGCAGAAACCAACAGCATGCGAATCGCGCGCATGGGAATCCTCATTGAGCGTTGAAAAGGCCTGGCGCCAc                                                                                                                                              >  2:459684/1‑150 (MQ=255)             ccAGCAACTGGGTCAGGCGTTGTACGTCTTGCTCACCGGCATAAGCAGTAACCGAGCCCAGGGTCAGGGCAGAAACCAACAGCATGCGAATCGCGCGCATGGGAATCCTCATTGAGCGTTGAAAAGGCCTGGCGCCAACGGTGGCGCcag                                                                                                                                  <  1:268585/150‑1 (MQ=255)             ccAGCAACTGGGTCAGGCGTTGTACGTCTTGCTCACCGGCATAAGCAGTAACCGAGCCCAGGGTCAGGGCAGAAACCAACAGCATGCGAATCGCGCGCATGGGAATCCTCATTGAGCGTTGAAAAGGCCTGGCGCCAACGGTGGCGCcag                                                                                                                                  <  1:428810/150‑1 (MQ=255)                  aaCTGGGTCAGGCGTTGTACGTCTTGCTCACCGGCATAAGCAGTAACCGAGCCCAGGGTCAGGGCAGAAACCAACAGCATGCGAATCGCGCGCATGGGAATCCTCATTGAGCGTTGAAAAGGCCTGGCGCCACCGTTGGCGCcaggcagg                                                                                                                             <  1:406360/150‑1 (MQ=255)                                                                ccGAGCCCAGGGTCAGGGCAGAAACCAACAGCATGCGAATCGCGCGCATGGGAATCCTCATTGAGCGTTGAAAAGGCCTGGCGCCACCGTTGGCGCCAGGCAGGGTGTTCATCAGTCGCGCGGGCCGCCCGGGGCTATCACTTCCCGCGa                                                                               <  1:459684/150‑1 (MQ=255)                                                                ccGAGCCCAGGGTCAGGGCAGAAACCAACAGCATGCGAATCGCGCGCATGGGAATCCTCATTGAGCGTTGAAAAGGCCTGGCGCCACCGTTGGCGCCAGGCAGGGTGTTCATCAGTCGCGCGGGCCGCCCGGGGCTATCACTTCCCGCGa                                                                               <  2:394287/150‑1 (MQ=255)                                                                                          aaCAGCATGCGAATCGCGCGCATGGGAATCCTCATTGAGCGTTGAAAAGGCCTGGCGCCAACGGTGGCGCcaggcctt                                                                                                                             <  1:110251/78‑4 (MQ=37)                                                                                          aaCAGCATGCGAATCGCGCGCATGGGAATCCTCATTGAGCGTTGAAAAGGCCTGGCGCCAACGGTGGCGCcaggcctt                                                                                                                             >  2:110251/1‑75 (MQ=37)                                                                                                        cgcgcgCATGGGAATCCTCATTGAGCGTTGAAAAGGCCTGGCGCCACCGTTGGCGCCAGGCAGGGTGTTCATCAGTCGCGCGGGCCGCCCGGGGCTATCACTTCCCGCGAGCCGTTGCTGTTCATCGGGGTGACAACGCCGGCCATCTcc                                       >  1:326408/1‑150 (MQ=255)                                                                                                             gcATGGGAATCCTCATTGAGCGTTGAAAAGGCCTGGCGCCACCGTTGGCGCCAGGCAGGGTGTTCATCAGTCGCGCGGGCCGCCCGGGGCTATCACTTCCCGCGAGCCGTTGCTGTTCATCGGGGTGACAACGCCGGCCATCTCCATCGa                                  >  1:481082/1‑150 (MQ=255)                                                                                                                         tCATTGAGCGTTGAAAAGGCCTGGCGCCACCGTTGGCGCCAGGCAGGGTGTTCATCAGTCGCGCGGGCCGCCCGGGGCTATCACTTCCCGCGAGCCGTTGCTGTTCATCGGGGTGACAACGCCGGCCATCTCCATCGACTCGATCATgcg                      >  1:475897/1‑150 (MQ=255)                                                                                                                                gCGTTGAAAAGGCCTGGCGCCACCGTTGGCGCCAGGCAGGGTGTTCATCAGTCGCGCGGGCCGCCCGGGGCTATCACTTCCCGCGAGCCGTTGCTGTTCATCGGGGTGACAACGCCGGCCATCTCCATCGACTCGATCATGCGCGCGGCa               >  1:393185/1‑150 (MQ=255)                                                                                                                                   ttGAAAAGGCCTGGCGCCACCGTTGGCGCCAGGCAGGGTGTTCATCAGTCGCGCGGGCCGCCCGGGGCTATCACTTCCCGCGAGCCGTTGCTGTTCATCGGGGTGACAACGCCGGCCATCTCCATCGACTCGATCATGCGCGCGGCACgg            <  2:393185/150‑1 (MQ=255)                                                                                                                                         aGGCCTGGCGCCACCGTTGGCGCCAGGCAGGGTGTTCATCAGTCGCGCGGGCCGCCCGGGGCTATCACTTCCCGCGAGCCGTTGCTGTTCATCGGGGTGACAACGCCGGCCATCTCCATCGACTCGATCATGCGCGCGGCACGGtt          >  1:423988/1‑146 (MQ=255)                                                                                                                                         aGGCCTGGCGCCACCGTTGGCGCCAGGCAGGGTGTTCATCAGTCGCGCGGGCCGCCCGGGGCTATCACTTCCCGCGAGCCGTTGCTGTTCATCGGGGTGACAACGCCGGCCATCTCCATCGACTCGATCATGCGCGCGGCACGGtt          <  2:423988/146‑1 (MQ=255)                                                                                                                                             cTGGCGCCAACGGTGGCGCCAGGCAGGGTGTTCATCAGTCGCGCGGGCCGCCCGGGGCTATCACTTCCCGCGAGCCGTTGCTGTTCATCGGGGTGACAACGCCGGCCATCTCCATCGACTCGATCATGCGCGCGGCACGGTTGTAGCCGa  >  2:290230/1‑150 (MQ=255)                                                                                                                                                      |                                                                                                                                             TCTGCGACTTTTCCAGCAACTGGGTCAGGCGTTGTACGTCTTGCTCACCGGCATAAGCAGTAACCGAGCCCAGGGTCAGGGCAGAAACCAACAGCATGCGAATCGCGCGCATGGGAATCCTCATTGAGCGTTGAAAAGGCCTGGCGCCACCGTTGGCGCCAGGCAGGGTGTTCATCAGTCGCGCGGGCCGCCCGGGGCTATCACTTCCCGCGAGCCGTTGCTGTTCATCGGGGTGACAACGCCGGCCATCTCCATCGACTCGATCATGCGCGCGGCACGGTTGTAGCCGA  >  NC_002947/4512357‑4512646

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑