| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 4,635,632 | A→T | 100% | M57K (ATG→AAG) | uvrY ← | BarA/UvrY two‑componenent system response regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 4,635,632 | 0 | A | T | 93.1% | 29.2 / ‑5.9 | 12 | M57K (ATG→AAG) | uvrY | BarA/UvrY two‑componenent system response regulator |
| Reads supporting (aligned to +/- strand): ref base A (0/1); new base T (5/6); total (5/7) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
CACCCTTGGTCAGGTAGCCGGCAGCGCCGGCCTGCAACAAGCGTGTGGGGAACGGGTCTTCCTCGCACACCGTCACCGCCACTACCTTGATGTCAGGGTGGCTGCGCAGCAGTTTGCGGGTGGCCTCCAGGCCGCCGATGCCGGGCATTTTCACATCCATCAGCACAACGTCCGGTTTCAGCTCCCGGGCCAGTTTCAGTGCCGACTCGCCGGAATCTCCCTCACCCACCACCTGCAGGCCGTCGATGTCGGCCAACATCCGTGTAATACCGGT > NC_002947/4635486‑4635759 | cACCCTTGGTCAGGTAGCCGGCAGCGCCGGCCTGCAACAAGCGTGTGGGGAACGGGTCTTCCTCGCACACCGTCACCGCCACTACCTTGATGTCAGGGTGGCTGCGCAGCAGTTTGCGGGTGGCCTCCAGGCCGCCGATGCCGGGCtttt < 1:84175/150‑1 (MQ=255) tGGTCAGGTAGCCGGCAGCGCCGGCCTGCAACAAGCGTGTGGGGAACGGGTCTTCCTCGCACACCGTCACCGCCACTACCTTGATGTCAGGGTGGCTGCGCAGCAGTTTGCGGGTGGCCTCCAGGCCGCCGATGCCGGGCTTTTTCAcat < 2:376464/150‑1 (MQ=255) tCAGGTAGCCGGCAGCGCCGGCCTGCAACAAGCGTGTGGGGAACGGGTCTTCCTCGCACACCGTCACCGCCACTACCTTGATGTCAGGGTGGCTGCGCAGCAGTTTGCGGGTGGCCTCCAGGCCGCCGATGCCGGGCTTTTTCAcatcca > 1:423454/1‑150 (MQ=255) tCACCGCCACTACCTTGATGTCAGGGTGGCTGCGCAGCAGTTTGCGGGTGGCCTCCAGGCCGCCGATGCCGGGCATTTTCACATCCATCAGCACAACGTCCGGTTTCAGCTCCCGGGCCAGTTTCAGTGCCGACTCGCCGGAATCTCCCt < 2:458952/150‑1 (MQ=255) cTTGATGTCAGGGTGGCTGCGCAGCAGTTTGCGGGTGGCCTCCAGGCCGCCGATGCCGGGCTTTTTCACATCCATCAGCACAACGTCCGGTTTCAGCTCCCGGGCCAGTTTCAGt < 1:483129/115‑1 (MQ=255) cTTGATGTCAGGGTGGCTGCGCAGCAGTTTGCGGGTGGCCTCCAGGCCGCCGATGCCGGGCTTTTTCACATCCATCAGCACAACGTCCGGTTTCAGCTCCCGGGCCAGTTTCAGt > 2:483129/1‑115 (MQ=255) cTTGATGTCAGGGTGGCTGCGCAGCAGTTTGCGGGTGGCCTCCAGGCCGCCGATGCCGGGCTTTTTCACATCCATCAGCACAACGTCCGGTTTCAGCTCCCGGGCCAGTTTCAGTGCCGACTCGCCGGAATCTCCCTCACCCACCACCTg > 2:16883/1‑150 (MQ=255) gATGTCAGGGTGGCTGCGCAGCAGTTTGCGGGTGGCCTCCAGGCCGCCGATGCCGGGCTTTTTCACATCCATCAGCACAACGTCCGGTTTCAGCTCCCGGGCCAGTTTCAGTGCCGACTCGCCGGAATCTCCCTCACCCACCACCTGCAg < 2:423454/150‑1 (MQ=255) gTCAGGGTGGCTGCGCAGCAGTTTGCGGGTGGCCTCCAGGCCGCCGATGCCGGGCTTTTTCACATCCATCAGCACAACGTCCGGTTTCAGCTCCCGGGCCAGttt < 1:6637/105‑1 (MQ=255) gTCAGGGTGGCTGCGCAGCAGTTTGCGGGTGGCCTCCAGGCCGCCGATGCCGGGCTTTTTCACATCCATCAGCACAACGTCCGGTTTCAGCTCCCGGGCCAGttt > 2:6637/1‑105 (MQ=255) cTCCAGGCCTCCGATGCCGGGCTTTTTCACATCCATAATGACAACGTCCGGTTTCAGCTCCCGGGCCAGTTTCAGTGCCGACTCGCCGGAATCTCCCTCACCCACCACCTGCAGGCCGTCGATGTCGGCCAACATCCGTGTAATACCGGt < 1:155573/150‑1 (MQ=255) ccgccgATGCCGGGCTTTTTCACATCCATCAGCACAACGTCCGGTTTCAGCTCCCGGGCCAGTTTCAGTGCCGACTCGCCGGAATCTcc > 1:174870/1‑89 (MQ=255) ccgccgATGCCGGGCTTTTTCACATCCATCAGCACAACGTCCGGTTTCAGCTCCCGGGCCAGTTTCAGTGCCGACTCGCCGGAATCTcc < 2:174870/89‑1 (MQ=255) | CACCCTTGGTCAGGTAGCCGGCAGCGCCGGCCTGCAACAAGCGTGTGGGGAACGGGTCTTCCTCGCACACCGTCACCGCCACTACCTTGATGTCAGGGTGGCTGCGCAGCAGTTTGCGGGTGGCCTCCAGGCCGCCGATGCCGGGCATTTTCACATCCATCAGCACAACGTCCGGTTTCAGCTCCCGGGCCAGTTTCAGTGCCGACTCGCCGGAATCTCCCTCACCCACCACCTGCAGGCCGTCGATGTCGGCCAACATCCGTGTAATACCGGT > NC_002947/4635486‑4635759 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |