Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	4,778,393	G→A	21.0%	Q307* (CAG→TAG)	pvdD ←	non‑ribosomal peptide synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	4,778,393	0	G	A	21.0%	33.2 / 4.3	19	Q307* (CAG→TAG)	pvdD	non‑ribosomal peptide synthetase
Reads supporting (aligned to +/- strand):  ref base G (9/6);  new base A (2/2);  total (11/8)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CTGTTGCAGCACATCGATGAAGCGGCTGTGCAGCGCGAAGTCGGCCTTGAGTACCTGGGTGTTGACGAAGAAACCGATCAACCCTTGCACTTCACTGCGGTTACGGTTGGCGATCGGTACGCCCACGCGCACCTGGGGCTGGCCGCTGTAGCGGTGCAGCAAGGTCTGGAACGATGCCAGCAACAGCATGAACAAGGTTACACCCTGGGCATTGGCCAACGCCTTGAGCGACTGCAGCAACACGGCATCGATCTCGATGCCCACCCGCGCGCCGCGCCCGCTG  >  NC_002947/4778253‑4778535                                                                                                                                             |                                                                                                                                               cTGTTGCAGCACATCGATGAAGCGGCTGTGCAGCGCGAAGTCGGACTAGACTACCTGGGTGTTGACGAAGAAACCGATCAACCCTTGCACTTCACTGCGGTTACGGTTGGCGATCGGTACGCCCACGCGCACCTGGGGCTGGCCGCTGTa                                                                                                                                       >  2:71331/1‑150 (MQ=255)              tCGATGAAGCGGCTGTGCAGCGCGAAGTCGGCCTTGAGTACCTGGGTGTTGACGAAGAAACCGATCAACCCTTGCACTTCACTGCGGTTACGGTTGGCGATCGGTACGCCCACGCGCACCTGGGGCTGGCCGCTGTAGCGGTGCAGCAAg                                                                                                                          >  2:488856/1‑150 (MQ=255)                gATGAAGCGGCTGTGCAGCGCGAAGTCGGCCTTGAGTACCTGGGTGTTGACGAAGAAACCGATCAACCCTTGCACTTCACTGCGGTTACGGTTGGCGATCGGTACGCCCACGCGCACCTGGGGCTGGCCGCTGTAGCGGTGCAGCAAGGt                                                                                                                        >  1:233157/1‑150 (MQ=255)                          cTGTGCAGCGCGAAGTCGGCCTTGAGTACCTGGGTGTTGACGAAGAAACCGATCAACCCTTGCACTTCACTGCGGTTACGGTTGGCGATCGGTACGCCCACGCGCACCTGGGGCTGGCCGCTGTAGCGGTGCAGCAAGGTCTGGAACGAt                                                                                                              <  1:429293/150‑1 (MQ=255)                                       aGTCGGCCTTGAGTACCTGGGTGTTGACGAAGAAACCGATCAACCCTTGCACTTCACTGCGGTTACGGTTGGCGATCGGTACGCCCACGCGCACCTGGGGCTGGCCGCTGTAGCGGTGCAGCAAGGTCTGGAACGATGCCAGCAACAGCa                                                                                                 <  1:488856/150‑1 (MQ=255)                                         tCGGCCTTGAGTACCTGGGTGTTGACGAAGAAACCGATCAACCCTTGCACTTCACTGCGGTTACGGTTGGCGATCGGTACGCCCACGCGCACCTGGGGCTAGCCGCTGTAGCGGTGCAGCAAGGTCTGGAACGATGCCAGCAACAGCATg                                                                                               >  1:118034/1‑150 (MQ=255)                                         tCGGCCTTGAGTACCTGGGTGTTGACGAAGAAACCGATCAACCATTGCACTTCACTGCGGTTACGGTTGGCGATCGGTACGCCCACGCGCACCTGGGGCTAGCCGCTGTAGCGGTGCAGCAAGGTCTGGAACGATGCCAGCAACAGCATg                                                                                               >  1:118040/1‑150 (MQ=255)                                                            tgtTGACGAAGAAACCGATCAACCCTTGCACTTCACTGCGGTTACGGTTGGCGATCGGTACGCCCACGCGCACCTGGGGCTGGCCGCTGTAGCGGTGCAGCAAGGTCTGGAACGATGCCAGCAACAGCATGAACAAGGTTACACCCTggg                                                                            <  1:71331/150‑1 (MQ=255)                                                                 aCGAAGAAACCGATCAACCCTTGCACTTCACTGCGGTTACGGTTGGCGATCGGTACGCCCACGCGCACCTGGGGCTGGCCGCTGTAGCGGTGCAGCAAGGTCTGGAACGATGCCAGCAACAGCATGAACAAGGTTACACCCTGGGCATTg                                                                       <  2:233157/150‑1 (MQ=255)                                                                          ccGATCAACCCTTGCACTTCACTGCGGTTACGGTTGGCGATCGGTACGCCCACGCGCACCTGGGGCTGGCCGCTGTAGCGGTGCAGCAAGGTCTGGAACGATGCCAGCAACAGCATGAACAAGGTTACACCCTGGGCATTGGCCAACGcc                                                              >  1:283982/1‑150 (MQ=255)                                                                              tCAACCCTTGCACTTCACTGCGGTTACGGATGGCGATCGGTACGCCCACGCGCACCTGGGGCTGGCCGCTGTAGCGGTGCAGCAAGGTCTGGAACGATGCCAGCAACAGCATGAACAAGGTTACACCCTGGGCATTGGCCAACGCCTTGa                                                          >  1:142566/1‑150 (MQ=255)                                                                                aaCCCTTGCACTTCACTGCGGTTACGGTTTGCGATCGGTACGCCCACGCGCACCTGGGGCTGGCCGCTGTAGCGGTGCAGCAAGGTCTGGAACGATGCCAGCAACAGCATGAACAAGGTTACACCCTGGGCATTGGCCAACGCCTTGAGc                                                        >  2:334340/1‑150 (MQ=255)                                                                                                  cggttacggttaGCGACCGGGACTTCGACGCGCACCTGGGGCTAGCCGCTGTAGCGGTGCAGCAAGGTCTGGAACGATGCCAGCAACAGCATGAACAAGGTTACACCCTGGGCATTGGCCAACGCCTTGAGCGACTGCAGCAACACGGCa                                      <  2:118040/150‑1 (MQ=255)                                                                                                  cGGTTACGGTTGGCGATCGGTACGTCCACGCGCACCTGGGGCTAGCCGCTGTAGCGGTGCAGCAAGGTCTGGAACGATGCCAGCAACAGCATGAACAAGGTTACACCCTGGGCATTGGCCAACGCCTTGAGCGACTGCAGCAACACGGCa                                      <  2:118034/150‑1 (MQ=255)                                                                                                                           ccACGCGCACCTGGGGCTGGCCGCTGTAGCGGTGCAGCAAGGTCTGGAACGATGCCAGCAACAGCATGAACAAGGTTACACCCTGGGCATTGGCCAACGCCTTGAGc                                                        <  1:460690/107‑1 (MQ=255)                                                                                                                           ccACGCGCACCTGGGGCTGGCCGCTGTAGCGGTGCAGCAAGGTCTGGAACGATGCCAGCAACAGCATGAACAAGGTTACACCCTGGGCATTGGCCAACGCCTTGAGc                                                        >  2:460690/1‑107 (MQ=255)                                                                                                                                      tGGGGCTGGCCGCTGTAGCGGTGCAGCAAGGTCTGGAACGATGCCAGCAACAGCATGAACAAGGTTACACCCTGGGCATTGGCCAACGCCTTGAGCGACTGCAGCAACACGGCATCGATCTCGATGCCCACCCGCGCGCCGCGCCCGCTg  >  1:162143/1‑150 (MQ=255)                                                                                                                                            tGGCCGCTGTAGCAGTGCAGCAAGGTCTGGAACGATGCCAGCAACAGCATGAACAAGGTTACACCCTGGGCATTGGCCAACGCCTTGAGCGa                                                      <  1:499508/92‑1 (MQ=255)                                                                                                                                            tGGCCGCTGTAGCAGTGCAGCAAGGTCTGGAACGATGCCAGCAACAGCATGAACAAGGTTACACCCTGGGCATTGGCCAACGCCTTGAGCGa                                                      >  2:499508/1‑92 (MQ=255)                                                                                                                                             |                                                                                                                                               CTGTTGCAGCACATCGATGAAGCGGCTGTGCAGCGCGAAGTCGGCCTTGAGTACCTGGGTGTTGACGAAGAAACCGATCAACCCTTGCACTTCACTGCGGTTACGGTTGGCGATCGGTACGCCCACGCGCACCTGGGGCTGGCCGCTGTAGCGGTGCAGCAAGGTCTGGAACGATGCCAGCAACAGCATGAACAAGGTTACACCCTGGGCATTGGCCAACGCCTTGAGCGACTGCAGCAACACGGCATCGATCTCGATGCCCACCCGCGCGCCGCGCCCGCTG  >  NC_002947/4778253‑4778535

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑