| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 4,804,688 | T→C | 17.4% | D142G (GAC→GGC) | PP_4233 ← | oxidoreductase small subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 4,804,688 | 0 | T | C | 17.4% | 44.1 / 3.7 | 23 | D142G (GAC→GGC) | PP_4233 | oxidoreductase small subunit |
| Reads supporting (aligned to +/- strand): ref base T (10/9); new base C (2/2); total (12/11) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.48e-01 | |||||||||||
GCTACGTACGAAGCCCATGTTCAACCCTCCTTGACCAGGCCGAGATCGTTGAGCACCTTGCGGGTGGCATCGTAGTAACGCACATAGCCTGTGCAGCGGCAGAT‑GTGGTGACCGAGGCTGGCCTCGATGCGGTCCTCCACTTCGCTCTTCTTCAACGGCTGGCGCTGGGCCGTTTCCACCAGCACGGTAGCGGCATTGACGAAGCCTGGTGCGCAGTAGCTGCACTGGAAGGCGAACAGGTCGACGAACTTCTGCTGGATCGGGTTGAGCGTCAGGTTGCCG > NC_002947/4804556‑4804837 | gcTACGTACGAAGCCCATGTTCAACCCTCCTTGACCAGGCCGAGATCGTTGAGCACCTTGCGGGTGGCATCGTAGTAACGCACATAGCCTGTGCAGCGGCAGAT‑GTGGTGACCGAGGCTGGCCTCGATGCGGTCCTCCACTTCGCtcttc < 2:225122/150‑1 (MQ=255) gTTCAACCCTCCTTGACCAGGCCGAGATCGTTGAGCACCTTGCGGGTGGCATCGTAGTAACGCACATAGCCTGTGCAGCGGCAGAT‑GTGGTGACCGAGGCTGGCCTCGATGCGGTCCTCCACTTCGCTCTTCTTCAACGGCTGGCGCTgg < 1:58116/150‑1 (MQ=255) aaCCCTCCTTGACCAGGCCGAGATCGTTGAGCACCTTGCGGGTGGCATCGTAGTCAAGCCCATAGCCTGTGCATAGCGGTAT‑GTGGTGACCGAGGCTGGCCTCGATGCGGTCCTCCACTTCGCTCTTCTTCAACGGCTGGCGCTGGGCCg > 1:316662/1‑150 (MQ=255) ctcctTGACCAGGCCGAGATCGTTGAGCACCTTGCGGGTGGCATCGTAGTAACGCACATAGCCTGTGCAGCGGCAGAT‑GTGGTGACCGAGGCTGGCCTCGATGCGGTCCTCCACTTCGCTCTTCTTCAACGGCTGGCGCTGGGCCGTTTc < 2:288724/150‑1 (MQ=255) aGGCCGAGATCGTTGAGCACCTTGCGGGTGGCATCGTAGTAACGCACATAGCCTGTGCAGCGGCAGAT‑GTGGTGACCGAGGCTGGCCTCGATGCGGTCCTCCACTTCGctc > 1:136623/1‑111 (MQ=255) aGGCCGAGATCGTTGAGCACCTTGCGGGTGGCATCGTAGTAACGCACATAGCCTGTGCAGCGGCAGAT‑GTGGTGACCGAGGCTGGCCTCGATGCGGTCCTCCACTTCGctc < 2:136623/111‑1 (MQ=255) cGAGATCGTTGAGCACCTTGCGGGTGGCATCGTAGTAACGCACATAGCCTGTGCAGCGGCAGAT‑GTGGTGACCGAGGCTGGCCTCGATGCGGTCCTCCACTTCGCTCTTCTTCAACGGCTGGCGCTGGGCCGTTTCCACCAGCACGGTAg > 2:441621/1‑150 (MQ=255) tCGTTGAGCACCTTGCGGGTGGCATCGTAGTAACGCACATAGCCTGTGCAGCGGCAGAT‑GTGGTGACCGAGGCTGGCCTCGATGCGGTCCTCCACTTCGCTCTTCTTCAACGGCTGGCGCTGGGCCGTTTCCACCAGCACGGTAGCGGCa > 1:68133/1‑150 (MQ=255) gTTGAGCACCTTGCGGGTGGCATCGTAGTAACGCACATAGCCTGTGCAGCGGCAGAT‑GTGGTGACCGAGGCTGGCCTCGATGCGGTCCTCCACTTCGCTCTTCTTCAACGGCTGGCGCTGGGCCGTTTCCACCAGCACGGTAGCGGCAtt < 2:403616/150‑1 (MQ=255) cACCTTGCGGGTGGCATCGTAGTAACGCACATAGCCTGTGCAGCGGCGGATCGCAGT‑ACCGAGGCTGGCCTCGATGCGGTCCTCCACTTCGCTCTTCTTCAACGGCTGGCGCTGGGCCGTTTCCACCAGCACGGTAGCGGCATTGACGaa > 1:291141/1‑150 (MQ=255) ggCATCGTAGTAACGCACATAGCCTGTGCAGCGGCAGAT‑GTGGTGACCGAGGCTGGCCTCGATGCGGTCCTCCACTTCGCTCTTCTTCAACGGCTGGCGCTGGGCCGTTTCCACCAGCACGGTAGCGGCATTGACGAAGCCTGGTGCGCa < 2:68133/150‑1 (MQ=255) tCGTAGTAACGCACATAGCCTGTGCAGCGGCAGAT‑GTGGTGACCGAGGCTGGCCTCGATGCGGCCCTCCACTTCGCTCTTCTTCAACGGCTGGCGCTGGGCCGTTTCCACCAGCAc < 1:306337/116‑1 (MQ=255) tCGTAGTAACGCACATAGCCTGTGCAGCGGCAGAT‑GTGGTGACCGAGGCTGGCCTCGATGCGGCCCTCCACTTCGCTCTTCTTCAACGGCTGGCGCTGGGCCGTTTCCACCAGCAc < 1:306290/116‑1 (MQ=255) tCGTAGTAACGCACATAGCCTGTGCAGCGGCAGAT‑GTGGTGACCGAGGCTGGCCTCGATGCGGCCCTCCACTTCGCTCTTCTTCAACGGCTGGCGCTGGGCCGTTTCCACCAGCAc > 2:306290/1‑116 (MQ=255) tCGTAGTAACGCACATAGCCTGTGCAGCGGCAGAT‑GTGGTGACCGAGGCTGGCCTCGATGCGGCCCTCCACTTCGCTCTTCTTCAACGGCTGGCGCTGGGCCGTTTCCACCAGCAc > 2:306337/1‑116 (MQ=255) tagtaACGCACATAGCCTGTGCAGCGGCAGAT‑GTGGTGACCGAGGCTGGCCTCGATGCGGTCCTCCACTTCGCTCTTCTTCAACGGCTGGCGCTGGGCCGTTTCCACCAGCACGGTAGCGGCATTGACGAAGCCTGGTGCGCAGTAGCTg > 2:101196/1‑150 (MQ=255) gCGGCAGAT‑GTGGTGACCGAGGCTGGCCTCGATGCGGTCCTCCACTTCGCTCTTCTTCAACGGCTGGCGCTGGGCCGTTTCCACCAGCACGGTAGCGGCATTGACGAAGCCTGGTGCGCAGTAGCTGCCCTGGAAGAGGAACAgcgctac > 2:267959/1‑144 (MQ=255) gaagctttggtgACCGAGGCTGGCCTCGATGCGGTCCTCCACTTCGCTCTTCTTCAACGGCTGGCGCTGGGCCGTTTCCACCAGCACGGTAGCGGCATTGACGAAGCCTGGTGCGCAGTAGCTGCACTGGAAGGCGAACAGGTCGACGaa < 2:291141/143‑1 (MQ=255) gtgACCGAGGCTGGCCTCGATGCGGTCCTCCACTTCGCTCTTCTTCAACGGCTGGCGCTGGGCCGTTTCCACCAGCACGGTAGCGGCATTGACGAAGCCTGGTGCGCAGTAGCTGCACTGGAAGGCGAACAGGTCGACGAACTTctgctg > 2:81037/1‑150 (MQ=255) aCCGAGGCTGGCCTCGATGCGGTCCTCCACTTCGCTCTTCTTCAACGGCTGGCGCTGGGCCGTTTCCACCAGCACGGTAGCGGCATTGACGAAGCCTGGTGCGCAGTAGCTGCACt < 2:162776/116‑1 (MQ=255) aCCGAGGCTGGCCTCGATGCGGTCCTCCACTTCGCTCTTCTTCAACGGCTGGCGCTGGGCCGTTTCCACCAGCACGGTAGCGGCATTGACGAAGCCTGGTGCGCAGTAGCTGCACt > 1:162776/1‑116 (MQ=255) cGAGGCTGGCCTCGATGCGGTCCTCCACTTCGCTCTTCTTCAACGGCTGGCGCTGGGCCGTTTCCACCAGCACGGTAGCGGCATTGACGAAGCCTGGTGCGCAGTAGCTGCACTGGAAGGCGAACAGGTCGACGAACTTCTGCTGGATCg > 2:464047/1‑150 (MQ=255) ggCTGGCCTCGATGCGGTCCTCCACTTCGCTCTTCTTCAACGGCTGGCGCTGGGCCGTTTCCACCAGCACGGTAGCGGCATTGACGAAGCCTGGTGCGCAGTAGCTGCACTGGAAGGCGAACAGGTCGACGAACTTCTGCTGGATCGGGt < 2:316662/150‑1 (MQ=255) tcctccACTTCGCTCTTCTTCAACGGCTGGCGCTGGGCCGTTTCCACCAGCACGGTAGCGGCATTGACGAAGCCTGGTGCGCAGTAGCTGCACTGGAAGGCGAACAGGTCGACGAACTTCTGCTGGATCGGGTTGAGCGTCAGGTTGCCg < 1:441621/150‑1 (MQ=255) | GCTACGTACGAAGCCCATGTTCAACCCTCCTTGACCAGGCCGAGATCGTTGAGCACCTTGCGGGTGGCATCGTAGTAACGCACATAGCCTGTGCAGCGGCAGAT‑GTGGTGACCGAGGCTGGCCTCGATGCGGTCCTCCACTTCGCTCTTCTTCAACGGCTGGCGCTGGGCCGTTTCCACCAGCACGGTAGCGGCATTGACGAAGCCTGGTGCGCAGTAGCTGCACTGGAAGGCGAACAGGTCGACGAACTTCTGCTGGATCGGGTTGAGCGTCAGGTTGCCG > NC_002947/4804556‑4804837 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |