| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 4,896,422 | C→T | 10.8% | intergenic (+10/‑198) | PP_4304 → / → sbp‑I | VIC family cation transporter/sulfate ABC transporter substrate‑binding protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 4,896,422 | 0 | C | T | 10.8% | 94.2 / 3.7 | 37 | intergenic (+10/‑198) | PP_4304/sbp‑I | VIC family cation transporter/sulfate ABC transporter substrate‑binding protein |
| Reads supporting (aligned to +/- strand): ref base C (17/16); new base T (2/2); total (19/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.25e-01 | |||||||||||
TCTTTACCGCCGAGCTGGCCAATGCCATGCGTGGGGAGCAGCTGCAGCATGATTGCCCGACCTGCCACAAGAAGACCCACGAGCAGGGGGCGGCGTTCTGTTCTCGCTGTGGGAATGGGTTGTTTCCCAAAGCGTGATAGCGCCTGCGCCGGCCTCTTCGCGGGTAAATCCGCTCCTACAGGCGCGGACCGTGCGCGGTCCCCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAGAAGCATAACCAAAGCATTTTTTGTTCTTTAACCCGTATAAACCCACCCGTTA > NC_002947/4896276‑4896571 | tCTTTACCGCCGAGCTGGCCAATGCCATGCGTGGGGAGCAGCTGCAGCATGATTGCCCGACCTGCCACAAGAAGACCCACGAGCAGGGGGCGGCGTTCTGTTCTCGCTGTGGGAATGGGTTGTTTCCCAAAGCGTGATAGCGCCTGCGCC > 1:45730‑M1/1‑150 (MQ=255) tctGGCCAATGCCATGCGTGGGGAGCGGCTGCAGCATGATTGCACGACCTGCCACAAGAAGACCCACGCGCAGGGGGCGGCGTTCTGTTCTCGCTGTGGGAATGGGTTGTTTCGCAAAGCGTGATAGCGCCTGCGCCGACCTCGGCGCGG < 2:67073‑M1/149‑1 (MQ=255) gCCAATGCCATGCGTGGGGAGCAGCTGCAGCATGATTGCCCGACCTGCCACAAGAAGACCCACGAGCAGGGGGCGGCGTTCTGTTCTCGCTGTGGGAATGGGTTGTTTCCCAAAGCGTGATAGCGCCTGCGCCGGCCTCTTCGCGGGTAA > 1:299051‑M1/1‑150 (MQ=255) gTGGGGAGCAGCTGCAGCATGATTGCCCGACCTGCCACAAGAAGACCCACGAGCAGGGGGCGGCGTTCTGTTCTCGCTGTGGGAATGGGTTGTTTCCCAAAGCGTGATAGCGCCTGCGCCGGCCTCTTCGCGGGTAAATCCGCTCCTACa > 1:456969/1‑150 (MQ=255) gcagcaTGATTGCCCGACCTGCCACAAGAAGACCCACGAGCAGGGGGCGGCGTTCTGTTCTCGCTGTGGGAATGGGTTGTTTCCCAAAGCGTGATAGCGCCTGCGCCGGCCTCTTCGCGGGTAAATCCGCTCCTACAGGCGCGGACCGTg > 2:135693/1‑150 (MQ=255) gCCCGACCTGCCACAAGAAGACCCACGAGCAGGGGGCGGCGTTCTGTTCTCGCTGTGGGAATGGGTTGTTTCCCAAAGCGTGATAGCGCCTGCGCCGGCCTCTTCGCGGGTAAATCCGCTCCTACAGGCGCGGACCGTGCGCGGTccccc < 2:45730/150‑1 (MQ=255) tGCCACAAGAAGACCCACGCGCAGGGGGCGGCGTTCTGTTCTCGCTGTGGGAATGGGTTGTTTCCCAAAGCGTGATAGCGCCTGCGCCGGCCTCTTCGCGGGTAAATCCGCTCCTACAGGCGCGGACCGTGCGCGGTCCCCCGTAGGAGc > 1:144782/1‑150 (MQ=255) tGCCACAAGAAGACCCACGAGCAGGGGGCGGCGTTCTGTTCTCGCTGTGGGAATGGGTTGTTTCCCAAAGCGTGATAGCGCCTGCGCCGGCCTCTTCGCGGGTAAATCCGCTCCTACAGGCGCGGACCGTGCGCGGTCCCCCGTAGGAGc > 1:353246/1‑150 (MQ=255) ccACAAGAAGACCCACGAGCAGGGGGCGGCGTTCTGTTCTCGCTGTGGGAATGGGTTGTTTCCCAAAGCGTGATAGCGCCTGCGCCGGCCTCTTCGCGGGTAAATCCGCTCCTACAGGCGCGGACCGTGCGCGGTCCCCCGTAGGAGCgg < 2:166565/150‑1 (MQ=255) cacaAGAAGACCCACGAGCAGGGGGCGGCGTTCTGTTCTCGCTGTGGGAATGGGTTGTTTCCCAAAGCGTGATAGCGCCTGCGCCGGCCTCTTCGCGGGTAAAtccgctcctacgggggaccgcgcacggtccgcgcctgtaggagcgga < 2:299051‑M1/150‑48 (MQ=255) gaCCCACGAGCAGGGGGCGGCGTTCTGTTCTCGCTGTGGGAATGGGTTGTTTCCCAAAGCGTGATAGCGCCTGCGCCGGCCTCTTCGCGGGTAAATCCGCTCCTACAGGCGCGGACCGTGCGCGGTCCCCCGTAGGAGCGGGtttacccg < 2:173499‑M2/150‑9 (MQ=255) ccACGAGCAGGGGGCGGCGTTCTGTTCTCGCTGTGGGAATGGGTTGTTTCCCAAAGCGTGATAGCGCCTGCGCCGGCCTCTTCGCGGGTAAATCCGCTc < 2:427080/99‑1 (MQ=255) ccACGAGCAGGGGGCGGCGTTCTGTTCTCGCTGTGGGAATGGGTTGTTTCCCAAAGCGTGATAGCGCCTGCGCCGGCCTCTTCGCGGGTAAATCCGCTc > 1:427080/1‑99 (MQ=255) ccACGAGCAGGGGGCGGCGTTCTGTTCTCGCTGTGGGAATGGGTTGTTTCCCAAAGCGTGATAGCGCCTGCGCCGGCCTCTTCGCGGGTAAATCCGCTCCTACAGGCGCGGACAGTGCGCGGTCCCCCGTAGGAGCgg > 2:338770/1‑138 (MQ=255) ccACGAGCAGGGGGCGGCGTTCTGTTCTCGCTGTGGGAATGGGTTGTTTCCCAAAGCGTGATAGCGCCTGCGCCGGCCTCTTCGCGGGTAAATCCGCTCCTACAGGCGCGGACAGTGCGCGGTCCCCCGTAGGAGCgg < 1:338770/138‑1 (MQ=255) ccACGAGCAGGGGGCGGCGTTCTGTTCTCGCTGTGGGAATGGGTTGTTTCCCAAAGCGTGATAGCGCCTGCGCCGGCCTCTTCGCGGGTAA < 1:223216‑M1/91‑1 (MQ=255) ccACGAGCAGGGGGCGGCGTTCTGTTCTCGCTGTGGGAATGGGTTGTTTCCCAAAGCGTGATAGCGCCTGCGCCGGCCTCTTCGCGGGTAA > 2:223216‑M1/1‑91 (MQ=255) cACGAGCAGGGGGCGGCGTTCTGTTCTCGCTGTGGGAATGGGTTGTTTCCCAAAGCGTGATAGCGCCTGCGCCGGCCTCTTCGCGGGTAAATCCGCTCCTACAGGCGCGGACCGCGCACGGTccg < 2:145466/125‑2 (MQ=255) cACGAGCAGGGGGCGGCGTTCTGTTCTCGCTGTGGGAATGGGTTGTTTCCCAAAGCGTGATAGCGCCTGCGCCGGCCTCTTCGCGGGTAAATCCGCTCCTACAGGCGCGGACCGCGCACGGTccg > 1:145466/1‑124 (MQ=255) aCGAGCAGGGGGCGGCGTTCTGTTCTCGCTGTGGGAATGGGTTGTTTCCCAAAGCGTGATAGCGCCTGCGCCGGCCTCTTCGCGGGTAAAcccgctcctacgggggaccgcgcacggtccgcgcctgtaggagcggatttacccgcgaa < 2:366562‑M1/149‑60 (MQ=255) aCGAGCAGGGGGCGGCGTTCTGTTCTCGCTGTGGGAATGGGTTGTTTCCCAAAGCGTGATAGCGCCTGCGCCGGCCTCTTCGCGGGTAAAcccgctcctacgggggaccgcgcacggtccgcgcctgtaggagcggatttacccgcgaa > 1:366562‑M1/1‑90 (MQ=255) gggggCGGCGTTCTGTTCTCGCTGTGGGAATGGGTTGTTTCCCAAAGCGTGATAGCGCCTGCGCCGGCCTCTTCGCGGGTAAATCCGCTCCTACAGGCGCGGACCGTGCGCGGTCCCCCGTAGGAGCGGGtttacccgcgaagaggccgg > 1:135693‑M2/1‑130 (MQ=255) ggggCGGCGTTCTGTTCTCGCTGTGGGAATGGGTTGTTTCCCAAAGCGTGATAGCGCCTGTGCCGGCCTCTTCGCGGGTAAAcccgctcctacgggggaccgcgcacggtccgcgcctgtaggagcggatttacccgcgaagaggccggc > 2:452999‑M1/1‑82 (MQ=255) gttctgttctCGCTGTGGGAATGGGTTGTTTCCCAAAGCGTGATAGCGCCTGCGCCGGCCTCTTCGCGGGTAAATCCGCTCCTACAGGCGCGGACCGTGCGCGGTCCCCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAg > 1:158673/1‑150 (MQ=255) gttctCGCTGTGGGAATGGGTTGTTTCCCAAAGCGTGATAGCGCCTGCGCCGGCCTCTTCGCGGGTAAATCCGCTCCTACAGCCGCGGACCGGGCGCGGTCCCCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAGAAGCa < 2:250841/150‑1 (MQ=255) tgtgGGAATGGGTTGTTTCCCAAAGCGTGATAGCGCCTGTGCCGGCCTCTTCGCGGGTAAAcccgctcctacgggggaccgcgcacggtccgcgcctgtaggagcggatttacccgcgaagaggccggcgcaggcgctatcacgctttgg < 1:452999‑M1/150‑90 (MQ=255) tgtgGGAATGGGTTGTTTCCCAAAGCGTGATAGCGCCTGCGCCGGCCTCTTCGCGGGTAAAcccgctcctacgggggaccgcgcacggtccgcgcctgtaggagcgga < 1:408324‑M1/108‑48 (MQ=255) tgtgGGAATGGGTTGTTTCCCAAAGCGTGATAGCGCCTGCGCCGGCCTCTTCGCGGGTAAAcccgctcctacgggggaccgcgcacggtccgcgcctgtaggagcgga > 2:408324‑M1/1‑61 (MQ=255) gAATGGGTTGTTTCCCAAAGCGTGATAGCGCCTGCGCCGGCCTCTTCGCGGGTAAAcccgctcctacgggggaccgcgcacggtccgcgcctgtaggagcggatttacc > 2:124205‑M1/1‑56 (MQ=255) gAATGGGTTGTTTCCCAAAGCGTGATAGCGCCTGCGCCGGCCTCTTCGCGGGTAAAcccgctcctacgggggaccgcgcacggtccgcgcctgtaggagcggatttacc < 1:124205‑M1/109‑54 (MQ=255) ccAAAGCGTGATAGCGCCTGCGCCGGCCTCTTCGCGGGTAAATCCGCTCCTACAGGCGCGGACCGTGCGCGGTCCCCCGTAGGAGCGGGtttacccgcgaagaggccggcacaggcgctatcacgctttgggaaacaacccattcccaca < 1:452999‑M2/150‑62 (MQ=255) cAAAGCGTGATAGCGCCTGCGCCGGCCTCTTCGCGGGTAAATCCGCTCCTACAGGCGCGGACCGTGCGCGGTCCCCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAGAAGCATAACCAAAGCATTTTTTGTTCTTTAAcc > 1:269647/1‑150 (MQ=255) aaaGCGTGATAGCGCCTGCGCCGGCCTCTTCGCGGGTAAATCCGCTCCTACAGGCGCGGACCGTGCGCGGTCCCCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAGAAGCATAACCAAAGCATTTTTTGTTCTTTAAccc < 2:265573/150‑1 (MQ=255) gTGATAGCGCCTGTGCCGGCCTCTTCGCGGGTAAAcccgctcctacgggggaccgcgcacggtccgcgcctgtaggagcggat > 2:175848‑M1/1‑35 (MQ=255) gTGATAGCGCCTGTGCCGGCCTCTTCGCGGGTAAAcccgctcctacgggggaccgcgcacggtccgcgcctgtaggagcggat < 1:175848‑M1/83‑49 (MQ=255) aTAGCGCCTGCGCCGGCCTCTTCGCGGGTAAATCCGCTCCTACAGGCGCGGACCGTGCGCGGTCCCCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAGAAGCATAACCAAAGCATTTTTTGTTCTTTAACCCGTATAAac < 1:173499/150‑1 (MQ=255) ccTGCGCCGGCCTCTTCGCGGGTAAATCCGCTCCTACAGGCGCGGACCGTGCGCGGTCCCCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAGAAGCATAACCAAAGCATTTTTTGTTCTTTAACCCGTATAAacccaccc > 2:501834/1‑150 (MQ=255) cgcCGGCCTCTTCGCGGGTAAATCCGCTCCTACAGGCGCGGACCGTGCGCGGTCCCCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAGAAGCATAACCAAAGCATTTTTTGTTCTTTAACCCGTATAAACCCACCCGTta < 1:501834/150‑1 (MQ=255) | TCTTTACCGCCGAGCTGGCCAATGCCATGCGTGGGGAGCAGCTGCAGCATGATTGCCCGACCTGCCACAAGAAGACCCACGAGCAGGGGGCGGCGTTCTGTTCTCGCTGTGGGAATGGGTTGTTTCCCAAAGCGTGATAGCGCCTGCGCCGGCCTCTTCGCGGGTAAATCCGCTCCTACAGGCGCGGACCGTGCGCGGTCCCCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAGAAGCATAACCAAAGCATTTTTTGTTCTTTAACCCGTATAAACCCACCCGTTA > NC_002947/4896276‑4896571 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |