| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 4,936,588 | C→T | 21.1% | G16E (GGG→GAG) | flhA ← | flagellar export pore protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 4,936,588 | 0 | C | T | 21.1% | 34.1 / 3.7 | 19 | G16E (GGG→GAG) | flhA | flagellar export pore protein |
| Reads supporting (aligned to +/- strand): ref base C (9/6); new base T (2/2); total (11/8) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.94e-01 | |||||||||||
GCGTATACACAGACCAGCAGCACCACGATCGACAGGGCGATGTTGAACGTGAAGAACACGTCGAGCAGGAACGGCGGTATCGGCAACATCATCATTGCCAACATCACCAGCAGCAACAGCGGCACCCCCAGGTTGCCCCGGCCGAGCCCGGCCAGGTTGTTGCGGGCGTTGCTGATTAACTGAGTGCGATCCACCGCGAGTCCTCTTGATGCAAAACTTTGACGCCCAGGGGGCGCCTGGGCGTGGCTTTGCAAGAAGCTTTCCAACTTTGGCAGACACCCACACG > NC_002947/4936441‑4936726 | gCGTATACACAGACCAGCAGCACCACGATCGACAGGGCGATGTTGAACGTGAAGAACACGTCGAGCAGGAACGGCGGTATCGGCAACATCATCATTGCCAACATCACCAGCAGCAACAGCGGCACCCCCAGGTTGCCCCGGCCGAGCCCg > 2:323107/1‑150 (MQ=39) cacaGACCAGCAGCACCACGATCGACAGGGCGATGTTGAACGTGAAGAACACGTCGAGCAGGAACGGCGGTATCGGCAACATCATCATTGCCAACATCACCAGCAGCAACAGCGGCACCCCCAGGTTGCCCCGGCCGAGCCCGGCCAGgt < 1:332534/150‑1 (MQ=39) acaGACCAGCAGCACCACGATCGACAGGGCGATGTTGAACGTGAAGAACACGTCGAGCAGGAACGGCGGTATCGGCAACATCATCATTGCCAACATCACCAGCAGCAACAGCGGCACCCCCAGGTTGCCCCGGCCGAGCCCGGCCAGgtt > 1:372516/1‑150 (MQ=37) cGACAGGGCGATGTTGAACGTGAAGAACACGTCGAGCAGGAACGGCGGTATCGGCAACATCATCATTGCCAACATCACCAGCAGCAACAGCGGCACCCCCAGGTTGCCCCGGCCGAGCCCGGCCAGGTTGTTGCGGGCGTTGCTGATTaa < 1:323107/150‑1 (MQ=39) ggCGATGTTGAACGTGAAGAACACGTCGAGCAGGAACGGCGGTATCGGCAACATCATCATTGCCAACATCACCAGCAGCAACAGCGGCACCCCCAGGTTGCCCCGGCCGAGCCCGGCCAGGTTGTTGCGGGCGTTGCTGATTAACTGAGt < 1:461837/150‑1 (MQ=38) gaagaaCACGTCGAGCAGGAACGGCGGTATCGGCAACATCATCATTGCCAACATCACCAGCAGCAACAGCGGCACCCCCAGGTTGCCCCGGCCGGGCTCGGccggg > 2:128713/1‑103 (MQ=14) gaagaaCACGTCGAGCAGGAACGGCGGTATCGGCAACATCATCATTGCCAACATCACCAGCAGCAACAGCGGCACCCCCAGGTTGCCCCGGCCGGGCTCGGccggg > 2:128709/1‑103 (MQ=255) gaagaaCACGTCGAGCAGGAACGGCGGTATCGGCAACATCATCATTGCCAACATCACCAGCAGCAACAGCGGCACCCCCAGGTTGCCCCGGCCGGGCTCGGCCggg < 1:128713/106‑1 (MQ=14) gaagaaCACGTCGAGCAGGAACGGCGGTATCGGCAACATCATCATTGCCAACATCACCAGCAGCAACAGCGGCACCCCCAGGTTGCCCCGGCCGGGCTCGGCCggg < 1:128709/106‑1 (MQ=14) cacGTCGAGCAGGAACGGCGGTATCGGCAACATCATCATTGCCAACATCACCAGCAGCAACAGCGGCACCCCCAGGTTGCCCCGGCCGAGCCCGGCCAGGTTGTTGCGGGCGTTGCTGATTAACTGAGTGCGATCCACCGCGAGTCctct > 2:361248/1‑150 (MQ=35) aCGGCGGTATCGGCAACATCATCATTGCCAACATCACCAGCAGCAACAGCGGCACCCCCAGGTTGCCCCGGCCGAGCCCGGCCAGGTTGTTGCGGGCGTTGCTGATTAACTGAGTGCGATCCACCGCGAGTCCTCTTGATGCAAAACttt < 2:372516/150‑1 (MQ=35) aaCATCACCAGCAGCAACAGCGGCACCCCCAGGTTGCCCCGGCCGAGCCCGGCCAGGTTGTTGCGGGCGTTGCTGATTAACTGAGTGCGATCCACCGCGAGTCCTCTTGATGCAAAACTTTGACGCCCAGGGGGCGCCTGGGCGTGGCtt > 2:369762/1‑150 (MQ=255) cATCACCAGCAGCAACAGCGGCACCCCCAGGTTGCCCCGGCCGAGCCCGGCCAGGTTGTTGCGGGCGTTGCTGATTAACTGAGTGCGATCCACCGCGAGTCCTCTTGATGCAAAACTTTGACGCCCAGGGGGCGCCTGGGCGTGGCTTTg > 2:256478/1‑150 (MQ=255) aTCACCAGCAGCAACAGCGGCACCCCCAGGTTGCCCCGGCCGAGCCCGGCCAGGTTGTTGCGGGCGTTGCTGATTAACTGAGTGCGATCCACCGCGAGTCCTCTTGATGCAAAACTTTGACGCCCAGGGGGCGCCTGGGCGTGGCTTTGc < 1:369762/150‑1 (MQ=255) ccAGCAGCAACAGCGGCACCCCCAGGTTGCCCCGGCCGAGCCCGGCCAGGTTGTTGCGGGCGTTGCTGATTAACTGAGTGCGATCCACCGCGAGTCCTCTTGATGCAAAACTTTGACGCCCAGGGGGCGCCTGGGCGTGGCttt < 1:296029/144‑1 (MQ=255) ccAGCAGCAACAGCGGCACCCCCAGGTTGCCCCGGCCGAGCCCGGCCAGGTTGTTGCGGGCGTTGCTGATTAACTGAGTGCGATCCACCGCGAGTCCTCTTGATGCAAAACTTTGACGCCCAGGGGGCGCCTGGGCGTGGCttt > 2:296029/1‑144 (MQ=255) cagcagCAACAGCGGCACCCCCAGGTTGCCCCGGCCGAGCCCGGCCAGGTTGTTGCGGGCGTTGCTGATTAACTGAGTGCGATACACAGCGAGTCCTCTTGATGCAAAACTTTTACGCCCAGTGGTGGCCTGAGCGTCCCTTTGCaataa > 2:50073/1‑150 (MQ=14) ccAGGTTGCCCCGGCCGAGCCCGGCCAGGTTGTTGCGGGCGTTGCTGATTAACTGAGTGCGATCCACCGCGAGTCCTCTTGATGCAAAACTTTGACGCCCAGGGGGCGCCTGGGCGTGGCTTTGCAAGAAGCTTTCCAACTTTGGCAGac > 1:435080/1‑150 (MQ=255) cccGGCCGAGCCCGGCCAGGTTGTTGCGGGCGTTGCTGATTAACTGAGTGCGATCCACCGCGAGTCCTCTTGATGCAAAACTTTGACGCCCAGGGGGCGCCTGGGCGTGGCTTTGCAAGAAGCTTTCCAACTTTGGCAGACACCCACACg > 1:7903/1‑150 (MQ=255) | GCGTATACACAGACCAGCAGCACCACGATCGACAGGGCGATGTTGAACGTGAAGAACACGTCGAGCAGGAACGGCGGTATCGGCAACATCATCATTGCCAACATCACCAGCAGCAACAGCGGCACCCCCAGGTTGCCCCGGCCGAGCCCGGCCAGGTTGTTGCGGGCGTTGCTGATTAACTGAGTGCGATCCACCGCGAGTCCTCTTGATGCAAAACTTTGACGCCCAGGGGGCGCCTGGGCGTGGCTTTGCAAGAAGCTTTCCAACTTTGGCAGACACCCACACG > NC_002947/4936441‑4936726 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |