| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 5,027,863 | C→A | 14.2% | A28D (GCT→GAT) | PP_4432 → | Xaa‑Pro aminopeptidase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 5,027,863 | 0 | C | A | 14.2% | 74.8 / 2.7 | 28 | A28D (GCT→GAT) | PP_4432 | Xaa‑Pro aminopeptidase |
| Reads supporting (aligned to +/- strand): ref base C (10/14); new base A (2/2); total (12/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.53e-01 | |||||||||||
AACTGAATCACCAGGCAACACAATAAAACCATACCCCAATCATGAGGGATCGATCATGTCTGAAATTGTTGTGAACCTTCCGTTTACACGGGAGGAGTACGCCCAGCGTATCGCCAAAGTCCGTGCAGAAATGCAGGCTAGGAACATTGAGCTGCTGCTGGTTACTGACCCTTCGAACATGGCTTGGCTGACCGGCTACGACGGTTGGTCGTTCTACGTCCACCAGTGCGTACTGCTGGCCATGGAAGGTGAGCCTGTTTGGTATGGCCGCAACCAAG > NC_002947/5027726‑5028003 | aacTGAATCACCAGGCAACACAATAAAACCATACCCCAATCATGAGGGATCGATCATGTCTGAAATTGTTGTGAACCTTCCGTTTACACGGGAGGAGTACGCCCAGCGTATCGCCAAAGTCCGTGCAGAAATGCAGGCTAGGAACATTGa > 1:385365/1‑150 (MQ=255) tCACCAGGCAACACAATAAAACCATACCCCAATCATGAGGGATCGATCATGTCTGAAATTGTTGTGAACCTTCCGTTTACACGGGAGGAGTACGCCCAGCGTATCGCCAAAGTCCGTGCAGAAATGCAGGCTAGGAACATTGAgctgctg < 2:385365/150‑1 (MQ=255) gCAACACAATAAAACCATACCCCAATCATGAGGGATCGATCATGTCTGAAATTGTTGTGAACCTTCCGTTTACACGGGAGGAGTACGCCCAGCGTATCGTCAAAGTCCGTGCAGAAATGCAGGCTAGGAACATTGAGCTGCTGCTGGTTa < 1:268068/150‑1 (MQ=255) cacaATAAAACCATACCCCAATCATGAGGGATCGATCATGTCTGAAATTGTTGTGAACCTTCCGTTTACACGGGAGGAGTACGCCCAGCGTATCGCCAAAGTCCGTGCAGAAATGCAGGCTAGGAACATTGAGCTGCTGCTGGTTACTGa < 2:277943/150‑1 (MQ=255) cacaATAAAACCATACCCCAATCATGAGGGATCGATCATGTCTGAAATTGTTGTGAACCTTCCGTTTACACGGGAGGAGTACGCCCAGCGTATCGCCAAAGTCCGTGCAGAAATGCAGGCTAGGAACATTGAGCTGCTGCTGGTTACTGa > 1:77889/1‑150 (MQ=255) aaTAAAACCATACCCCAATCATGAGGGATCGATCATGTCCGAAATTGTTGTGAACCTTCCGTTTACACGGGAGGAGTACGCCCAGCGTATCGCCAAAGTCCGTGCAGAAATGCAGGCTAGGAACATTGAGCTGCTGCTGGTTACTGAccc > 2:145517/1‑150 (MQ=255) aaTAAAACCATACCCCAATCATGAGGGATCGATAATGTCCGAAACAATAGTGCACGTTCCTTTTACACGGAAGGAGTACGCCCAGCGTATCGCCAAAGTCCGTGCAGAAATGCAGGATAGGAACATTGAGCTGCTGCTGGTTACTGAccc > 1:9912/1‑150 (MQ=255) aTAAAACCATACCCCAATCATCAGGGATCGATCATGTCTGAAATTGTTGTGAACCTTCCGTTTACACGGGAGGAGTACGCCCAGCGTATCGCCAAAGTCCGTGCAGAAATGCAGGCTAGGAACATTGAGCTGCTGCTGGTTACTGACCCt < 1:501920/150‑1 (MQ=255) aTGTCTGAAATTGTTGTGAACCTTCCGTTTACACGGGAGGAGTACGCCCAGCGTATCGCCAAAGTCCGTGCAGAAATGCAGGCTAGGAACATTGAGCTGCTGCTGGTTACTGACCCTTCGAACATGGCTTGGCTGACCGGCTACGACGGt < 2:475291/150‑1 (MQ=255) tCTGAAATTGTTGTGAACCTTCCGTTTACACGGGAGGAGTACGCCCAGCGTATCGCCAAAGTCCGTGCAGAAATGCAGGCTAGGAACATTGAGCTGCTGCTGGTTACTGACCCTTCGAACATGGCTTGGCTGACCGGCTACGACGGTTgg > 2:63027/1‑150 (MQ=255) cTGAAATTGTTGTGAACCTTCCGTTTACACGGGAGGAGTACGCCCAGCGTATCGCCAAAGTCCGTGCAGAAATGCAGGCTAGGAACATTGAGCTGCTGCTGGTTACTGACCCTTCGAACATGGCTTGGCTGACCGGCTACGACGGTTGGt < 1:63027/150‑1 (MQ=255) aaaTTGTTGTGAACCTTCCGTTTACACGGGAGGAGTACGCCCAGCGTATCGCCAAAGTCCGTGCAGAAATGCAGGCTAGGAACATTGAGCTGCTGCTGGTTACTGACCCTTCGAACATGGCTTGGCTGACCGGCTACGACGGTTGGTCGt < 1:153278/150‑1 (MQ=255) aTTGTTGTGAACCTTCCGTTTACACGGGAGGAGTACGCCCAGCGTATCGCCAAAGTCCGTGCAGAAATGCAGGCTAGGAACATTGAGCTGCTGCTGGTTACTGACCCTTCGAACATGGCTTGGCTGACCGGCTACGACGGTTGGTCGTTc < 2:269391/150‑1 (MQ=255) tgAACCTTCCGTTTACACGGGAGGAGTACGCCCAGCGTATCGCCAAAGTCCGTGCAGAAATGCAGGCTAGGAACATTGAGCTGCTGCTGGTTACTGACCCTTCGAACATGGCTTGGCTGACCGGCTACGACGGTTGGTCGTTCTACGTcc > 1:494166/1‑150 (MQ=255) ttCAGTTTACACGGGAGTAGTACGCCCAGCGTATCGCCAAAGTCCGTGCAGAAATGCAGGATAGGAACATTGAGCTGTTTCTGGTTACTGGCCCTTCGAACATGGCTTGGCTGACCGGCTACGACGGTTGGTCGTTCTACGTCCACCAGt < 2:9912/150‑1 (MQ=255) acGGGAGGAGTACGCCCAGCGTATCGCCAAAGTCCGTGCAGAAATGCAGGCTAGGAACATTGAGCTGCTGCTGGTTACTGACCCTTCGAACATGGCTTGGCTGACCGGCTACGACGGTTgg > 1:368327/1‑121 (MQ=255) acGGGAGGAGTACGCCCAGCGTATCGCCAAAGTCCGTGCAGAAATGCAGGCTAGGAACATTGAGCTGCTGCTGGTTACTGACCCTTCGAACATGGCTTGGCTGACCGGCTACGACGGTTgg < 2:368327/121‑1 (MQ=255) aggagTACGCCCAGCGTATCGCCAAAGTCCGTGCAGAAATGCAGGATAGGAACATTGAGCTGCTGCTGGTTACTGACCCTTCGAACATGGCTTGGCTGACCGGCTACGACGGTTg > 1:507767/1‑115 (MQ=255) aggagTACGCCCAGCGTATCGCCAAAGTCCGTGCAGAAATGCAGGATAGGAACATTGAGCTGCTGCTGGTTACTGACCCTTCGAACATGGCTTGGCTGACCGGCTACGACGGTTg < 2:507767/115‑1 (MQ=255) ccAGCGTATCGCCAAAGTCCGTGCAGAAATGCAGGCTAGGAACATTGAGCTGCTGCTGGTTACTGACCCTTCGAACATGGCTTGGCTGACCGGCTa > 2:165800/1‑96 (MQ=255) ccAGCGTATCGCCAAAGTCCGTGCAGAAATGCAGGCTAGGAACATTGAGCTGCTGCTGGTTACTGACCCTTCGAACATGGCTTGGCTGACCGGCTa < 1:165800/96‑1 (MQ=255) aGCGTATCGCCAAAGTCCGTGCAGAAATGCAGGCTAGGAACATTGAGCTGCTGCTGGTTACTGACCCTTCGAACATGGCTTGGCTGACCGGCTACGACGGTTGGTCGTTCTACGTCCACCAGTGCGTACTGCTGGCCATGGAAGGTGAGc < 2:77889/150‑1 (MQ=255) gTATCGCCAAAGTCCGTGCAGAAATGCAGGCTAGGAACATTGAGCTGCTGCTGGTTACTGAccc < 1:383207/64‑1 (MQ=255) gTATCGCCAAAGTCCGTGCAGAAATGCAGGCTAGGAACATTGAGCTGCTGCTGGTTACTGAccc > 2:383207/1‑64 (MQ=255) tCCGTGCAGAAATGCAGGCTAGGAACATTGAGCTGCTGCTGGTTACTGACCCTTCGAACATGGCTTGGCTGACCGGCTACGACGGTTGGTCGTTCTACGTCCACCAGTGCGTACTGCTGGCCATGGAAGGTGAGc > 2:280172/1‑135 (MQ=255) tCCGTGCAGAAATGCAGGCTAGGAACATTGAGCTGCTGCTGGTTACTGACCCTTCGAACATGGCTTGGCTGACCGGCTACGACGGTTGGTCGTTCTACGTCCACCAGTGCGTACTGCTGGCCATGGAAGGTGAGc < 1:280172/135‑1 (MQ=255) ccGTGCAGAAATGCAGGCTAGGAACATTGAGCTGCTGCTGGTTACTGACCCTTCGAACATGGCTTGGCTGACCGGCTACGACGGTTGGTCGTTCTACGTCCACCAGTGCGTACTGCTGGCCATGGAAGGTGAGCCTGTTTGGTATGGCCg > 1:141683/1‑150 (MQ=255) aaaTGCAGGCTAGGAACATTGAGCTGCTGCTGGTTACTGACCCTTCGAACATGGCTTGGCTGACCGGCTACGACGGTTGGTCGTTCTACGTCCACCAGTGCGTACTGCTGGCCATGGAAGGTGAGCCTGTTTGGTATGGCCGCAACCAAg < 2:494166/150‑1 (MQ=255) | AACTGAATCACCAGGCAACACAATAAAACCATACCCCAATCATGAGGGATCGATCATGTCTGAAATTGTTGTGAACCTTCCGTTTACACGGGAGGAGTACGCCCAGCGTATCGCCAAAGTCCGTGCAGAAATGCAGGCTAGGAACATTGAGCTGCTGCTGGTTACTGACCCTTCGAACATGGCTTGGCTGACCGGCTACGACGGTTGGTCGTTCTACGTCCACCAGTGCGTACTGCTGGCCATGGAAGGTGAGCCTGTTTGGTATGGCCGCAACCAAG > NC_002947/5027726‑5028003 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |