| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 5,257,199 | C→T | 20.0% | Q217Q (CAG→CAA) | PP_4634 ← | alpha/beta family hydrolase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 5,257,199 | 0 | C | T | 20.0% | 35.6 / 4.7 | 20 | Q217Q (CAG→CAA) | PP_4634 | alpha/beta family hydrolase |
| Reads supporting (aligned to +/- strand): ref base C (9/7); new base T (2/2); total (11/9) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.75e-01 | |||||||||||
CAATTTCCTCGGCCGAGATATCCACAGGTGCAATGCGCAGGTGCTTTCGCTCGGCACCCTGTAGATAACCCAACAAACGCTCGGTCGCCGCCACGGTCCCGAAAGGATCGTCAGTCAGACTGATGGCCAACGTCGTCGCACGCACCTGGGCGAAAGGTAGCGCCGCCAACGCCCGCCCGCTGGGGCGGTGCTCATAACGTGGGGTACGCGTGGTCCAGTCGTGCACCACGCCGGATGGCGTGTCTTCCAGCCACCCCAGGCGCTTGCCGGGAAAATAGCCGAATACCCGGGTC > NC_002947/5257054‑5257346 | cAATTTCCTCGGCCGAGATATCCACAGGTGCAATGCGCAGGTGCTTTCGCTCGGCACCCTGTAGATAACCCAACAAACGCTCGGTCGCCGCCACGGTCCCGAAAGGATCGTCAGTCAGACTGATGGCCAACGTCGTCGCACGCACTTggg > 1:398130/1‑150 (MQ=255)cAATTTCCTCGGCCGAGATATCCACAGGTGCAATGCGCAGGTGCTTTCGCTCGGCACCCTGTAGATAACCCAACAAACGCTCGGTCGCCGCCACGGTCCCGAAAGGATCGTCAGTCAGACTGATGGCCAACGTCGTCGCACGCACTTggg > 1:398132/1‑150 (MQ=255) gaTATCCACAGGTGCAATGCGCAGGTGCTTTCGCTCGGCACCCTGTAGATAACCCAACAAACGCTCGGTCGCCGCCACGGTCCCGAAAGGATCGTCAGTCAGACTGATGGCCAACGTCGTCGCACGCACCTGGGCGAAAGGTAGcgccgc < 2:131126/150‑1 (MQ=255) atatCCACAGGTGCAATGCGCAGGTGCTTTCGCTCGGCACCCTGTAGATAACCCAACAAACGCTCGGTCGCCGCCACGGTCCCGAAAGGATCGTCAGTCAGACTGATGGCCAACGTCGTCGCACGCACCTGGGCGAAAGGTAGCgccgcc > 2:280713/1‑150 (MQ=255) acaGGTGCAATGCGCAGGTGCTTTCGCTCGGCACCCTGTAGATAACCCAACAAACGGTCGGTCGCCGCCACGGTCCCGAAAGGATCGTCAGTCAGACTGATGGCCAACGTCGTCGCACGCACCTGGGCGAAAGGTAGCGCCGCCAAcgcc > 2:329271/1‑150 (MQ=255) acaGGTGCAATGCGCAGGTGCTTTCGCTCGGCACCCTGTAGATAACACAACAAACGCTCGGTCGCCGCCACGGTCCCGAAAGGATCGTCAGTAAGACTGATGGCCAACGTCGTCGCACGCACCTGGGCGAAAGGTAGCGCCGCCAAcgcc > 1:220492/1‑150 (MQ=255) caGGTGCAATGCGCAGGTGCTTTCGCTCGGCACCCTGTAGATAACCCAACAAACGCTCGGTCGCCGCCACGGTCCCGAAAGGATCGTCAGTCAGACTGATGGCCAACGTCGTCGCACGCACTTGGGCGAAAGGTAGCGCCGCCAAcgccc < 2:398132/150‑1 (MQ=255) caGGTGCAATGCGCAGGTGCTTTCGCTCGGCACCCTGTAGATAACCCAACAAACGCTCGGTCGCCGCCACGGTCCCGAAAGGATCGTCAGTCAGACTGATGGCCAACGTCGTCGCACGCACTTGGGCGAAAGGTAGCGCCGCCAAcgccc < 2:398130/150‑1 (MQ=255) aTGCGCAGGTGCTTTCGCTCGGCACCCTGTAGATAACCCAACAAACGCTCGGTCGCCGCCACGGTCCCGAAAGGATCGTCAGTCAGACTGATGGCCAACGTCGTCGCACGCACCTGGGCGAAAGGTAGCGCCGCCAACGCCCGCCCGCTg < 2:229403/150‑1 (MQ=255) tttCGCTCGGCACCCTGTAGATAACCCAACAAACGCTCGGTCGCCGCCACGGTCCCGAAAGGATCGTCAGTCAGACTGATGGCCAACGTCGTCGCACGCACCTGGGCGAAAGGTAGCGCCGCCAACGCCCGCCCGCTGGGGCGGt < 1:315998/145‑1 (MQ=255) tttCGCTCGGCACCCTGTAGATAACCCAACAAACGCTCGGTCGCCGCCACGGTCCCGAAAGGATCGTCAGTCAGACTGATGGCCAACGTCGTCGCACGCACCTGGGCGAAAGGTAGCGCCGCCAACGCCCGCCCGCTGGGGCGGt > 2:315998/1‑145 (MQ=255) tCGCTCGGCACCCTGTAGATAACCCAACAAACGCTCGGTCGCCGCCACGGTCCCGAAAGGATCGTCAGTCAGACTGATGGCCAACGTCGTCGCACGCACCTGGGCGAAAGGTAGCGCCGCCAACGCCCGCCCGCTGGGGCGGTGCTCATa < 2:37351/150‑1 (MQ=255) cTGTAGATAACCCAACAAACGCTCGGTCGCCGCCACGGTCCCGAAAGGATCGTCAGTCAGACTGATGGCCAACGTCGTCGCACGCACCTGGGCGAAAGGTAGCGCCGCCAACGCCCGCCCGCTGGGGCGGTGCTCATAACGTGGGGTAcg > 1:119792/1‑150 (MQ=255) cgccgcCACGGTCCCGAAAGGATCGTCAGTCAGACTGATGGCCAACGTCGTCGCACGCACCTGGGCGAAAGGTAGCGCCGCCAACGCCCGCCCGCTGGGGCGGTGCTCATAACGTGGGGTACGCGTGGTCCAGTCGTGCACCACGCCGGa < 1:280713/150‑1 (MQ=255) tCCCGAAAGGATCGTCAGTCAGACTGATGGCCAACGTCGTCGCACGCACCTGGGCGAAAGGTAGCGCCGCCAACGCCCGCCCGCTGGGGCGGTGCTCATAACGTGGGGTACGCGTGGTCCAGTCGTGCACCACGCCGGATGGCGTGTCtt > 2:15129/1‑150 (MQ=255) cagtcagACTGATGGCCAACGTCGTCGCACGCACCTGGGCGAAAGGTAGCGCCGCCAACGCCCGCCCGCTGGGGCGGTGCTCATAACGTGGGGTACGCGTGGTCCAGTCGTGCACCACGCCGGATGGCGTGTCTTCCAGCCACCCCAGgc < 1:329271/150‑1 (MQ=255) agACTGATGGCCAACGTCGTCGCACGCACCTGGGCGAAAGGTAGCGCCGCCAACGCCCGCCCGCTGGGGCGGTGCTCATAACGTGGGGTACGCGTGGTCCAGTCGTGCACCACGCCGGATGGCGTGTCTTCCAGCCACCCCAGGCGCTTg > 2:201008/1‑150 (MQ=255) cgtcgCACGCACCTGGGCGAAAGGTAGCGCCGCCAACGCCCGCCCGCTGGGGCGGTGCTCATAACGTGGGGTACGCGTGGTCCAGTCGTGCACCACGCCGGATGGCGTGTCTTCCAGCCACCCCAGGCGCTTGCCGGGAAAATa > 2:258840/1‑144 (MQ=255) cgtcgCACGCACCTGGGCGAAAGGTAGCGCCGCCAACGCCCGCCCGCTGGGGCGGTGCTCATAACGTGGGGTACGCGTGGTCCAGTCGTGCACCACGCCGGATGGCGTGTCTTCCAGCCACCCCAGGCGCTTGCCGGGAAAATa < 1:258840/144‑1 (MQ=255) acCTGGGCGAAAGGTAGCGCCGCCAACGCCCGCCCGCTGGGGCGGTGCTCATAACGTGGGGTACGCGTGGTCCAGTCGTGCACCACGCCGGATGGCGTGTCTTCCAGCCACCCCAGGCGCTTGCCGGGAAAATAGCCGAATACCCGGGTc > 2:285715/1‑150 (MQ=255) | CAATTTCCTCGGCCGAGATATCCACAGGTGCAATGCGCAGGTGCTTTCGCTCGGCACCCTGTAGATAACCCAACAAACGCTCGGTCGCCGCCACGGTCCCGAAAGGATCGTCAGTCAGACTGATGGCCAACGTCGTCGCACGCACCTGGGCGAAAGGTAGCGCCGCCAACGCCCGCCCGCTGGGGCGGTGCTCATAACGTGGGGTACGCGTGGTCCAGTCGTGCACCACGCCGGATGGCGTGTCTTCCAGCCACCCCAGGCGCTTGCCGGGAAAATAGCCGAATACCCGGGTC > NC_002947/5257054‑5257346 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |