| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 5,346,315 | C→A | 14.7% | V555V (GTC→GTA) | acsA‑II → | acetyl‑CoA synthetase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 5,346,315 | 0 | C | A | 14.7% | 84.2 / 6.4 | 34 | V555V (GTC→GTA) | acsA‑II | acetyl‑CoA synthetase |
| Reads supporting (aligned to +/- strand): ref base C (14/15); new base A (2/3); total (16/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.23e-01 | |||||||||||
CTGGATCACCGGGCGCATCGACGATGTGATCAACGTGTCCGGGCACCGCATCGGCACCGCTGAAGTGGAAAGTGCACTGGTGCTGCATGACAGCGTGGCCGAAGCCGCGGTGGTCGGCTACCCCCACGACCTCAAGGGCCAAGGCGTCTACGCCTTCGTCACGACCATGAATGGCGTGACACCAGATGACACGCTCAAGGCCGAGCTGCTGGCCCTGGTCAGCAAGGAAATCGGCAGCTTCGCCAAGCCTGAGCTGATTCAGTGGGCGCCAGCGCTACCCAAGACGCGCTCAG > NC_002947/5346168‑5346460 | ctGGATCACCGGGCGCATCGACGATGTGATCAACGTGTCCGGGCACCGCATCGGCACCGCTGAAGTGGAAAGTGCACTGGTGCTGCATGACAGCGTGGCCGAAGCCGCGGTGGTCGGCTACCCCCACGACCTCAAGGGCCAAGGCGTCTa > 2:242712/1‑150 (MQ=255) tGGATCACCGGGCGCATCGACGATGTGATCAACGTGTCCGGGCACCGCATCGGCACCGCTGAAGTGGAAAGTGCACTGGTGCTGCATGACAGCGTGGCCGAAGCCGCGGTGGTCGGCTACCCCCACGACCTCAAGGGCCAAGGCGTCTAc < 1:117465/150‑1 (MQ=255) tGGATCACCGGGCGCATCGACGATGTGATCAACGTGTCCGGGCACCGCATCGGCACCGCTGAAGTGGAAAGTGCACTGGTGCTGCATGACAGCGTGGCCGAAGCCGCGGTGGTCGGCTACCCCCACGACATCAAGGGCCAAGGCGTCTAc > 2:117465/1‑150 (MQ=255) aCCGGGCGCATCGACGATGTGATCAACGTGTCCGGGCACCGCATCGGCACCGCTGAAGTGGACAGTGAACTGGTGCTGCATGACAGCGTGGCCGAAGCCGCGGTGGTCGGCGACCCCCATGACCTCAAGGGCCAAGGCGTAGACGCCttg < 2:186387/150‑2 (MQ=255) aCCGGGCGCATCGACGATGTGATCAACGTGTCCGGGCACCGCATCGGCACCGCTGAAGTGGAAAGTGCACTGGTGCTGCATGACAGCGTGGCCGAAGCCGCGGTGGTCGGCTACCCCCACGACCTCAAGGGCCAAGGCGTCTACGCCTTc < 1:141894/150‑1 (MQ=255) ccGGGCGCATCGACGATGTGATCAACGTGTCCGGGCACCGCATCGGCACCGCTGAAGTGGAAAGTGCACTGGTGCTGCATGACAGCGTGGCCGAAGCCGCGGTGGTCGGCTACCCCCACGACCTCAAGGGCCAAGGCGTCTACGCCTTCg < 1:242712/150‑1 (MQ=255) gtgATCAACGTGTCCGGGCACCGCATCGGCACCGCTGAAGTGGAAAGTGCACTGGTGCTGCATGACAGCGTGGCCGAAGCCGCGGTGGTCGGCTACCCCCACGACCTCAAGGGCCAAGGCGTCTACGCCTTCGTCACGACCATGAATGGc > 2:268668/1‑150 (MQ=255) tgATCAACGTGTCCGGGCACCGCATCGGCAACGCTGAAGTGGAAAGTGCACTGGTGCTGCATGACAGCGTGGCCGAAGCCGCGGTGGTCGGATACCCCCACGACCTCAAGGGCCAAGGCGTCTACGCCTTCGTCACGACCATGAATGGCg > 1:506286/1‑150 (MQ=255) tCAACGTGTCCGGGCACCGCATCGGCACCGCTGAAGTGGAAAGTGCACTGGTGCTGCATGACAGCGTGGCCGAAGCCGCGGTGGTCGGCTACCCCCACGACCTCAAGGGCCAAGGCGTCTACGCCTTCGTCACGACCATGAATGGCGTGa < 1:408691/150‑1 (MQ=255) aCGTGTCCGGGCACCGCATCGGCACCGCTGAAGTGGAAAGTGCACTGGTGCTGCATGACAGCGTGGCCGAAGCCGCGGTGGTCGGCTACCCCCACGACCTCAAGGGCCAAGGCGTCTACGCCTTCGTCACGACCATGAATGGCGTGacac > 1:147062/1‑150 (MQ=255) tgtCCGGGCACCGCATCGGCACCGCTGAAGTGGAAAGTGCACTGGTGCTGCATGACAGCGTGGCCGAAGCCGCGGTGGTCGGCTACCCCCACGACCTCAAGGGCCAAGGCGTCTACGCCTTCGTCACGACCATGAATGGCGTGACACCAg < 1:121251/150‑1 (MQ=255) tCCGGGCACCGCATCGGCACCGCTGAAGTGGAAAGTGCACTGGTGCTGCATGACAGCGTGGCCGAAGCCGCGGTGGTCGGCTACCCCCACGACCTCAAGGGCCAAGGCGTCTACGCCTTCGTCACGACCATGAATGGCGTGACACCAGAt > 1:28912/1‑150 (MQ=255) aCCGCATCGGCACCGCTGAAGTGGAAAGTGCACTGGTGCTGCATGACAGCGTGGCCGAAGCCGCGGTGGTCGGCTACCCCCACGACCTCAAGGGCCAAGGCGTAGACGCCttg < 1:302274/113‑2 (MQ=255) aCCGCATCGGCACCGCTGAAGTGGAAAGTGCACTGGTGCTGCATGACAGCGTGGCCGAAGCCGCGGTGGTCGGCTACCCCCACGACCTCAAGGGCCAAGGCGTAGACGCCttg > 2:302274/1‑112 (MQ=255) tCGGCACCGCTGAAGTGGAAAGTGCACTGGTGCTGCATGACAGCGTGGCCGAAGCCGCGGTGGTCGGCTACCCCCACGACCTCAAGGGCCAAGGCGTCTACGCCTTCGTCACGACCATGAATGGCGTGACACCAGATGACACGCTCAAgg < 1:127257/150‑1 (MQ=255) gCACCGCTGAAGTGGAAAGTGCACTGGTGCTGCATGACAGCGTGGCCGAAGCCGCGGTGGTCGGCTACCCCCACGACCTCAAGGGCCAAGGCGTCTACGCCTTCGTCACGACCATGAATGGCGTGACACCAGATGACACGCTCAAGGCCg > 2:461632/1‑150 (MQ=255) tGGTGCTGCATGACAGCGTGGCCGAAGCCGCGGTGGTCGGCTACCCCCACGACCTCAAGGGCCAAGGCGTCTACGCCTTc < 2:92129/80‑1 (MQ=255) tGGTGCTGCATGACAGCGTGGCCGAAGCCGCGGTGGTCGGCTACCCCCACGACCTCAAGGGCCAAGGCGTCTACGCCTTc > 1:92129/1‑80 (MQ=255) gctgcATGACAGCGTGGCCGAAGCCGCGGTGGTCGGCTACCCCCACGACCTCAAGGGCCAAGGCGTCTACGCCTTCGTCACGACCATGAATGGCGTGACACCAGATGACACGCTCAAGGCCGAGCTGCTGGCCCTGGTCAGCAAGGAAAt > 1:21093/1‑150 (MQ=255) ctgcATGACAGCGTGGCCGAAGCCGCGGTGGTCGGCTACCCCCACGACCTCAAGGGCCAAGGCGTCTACGCCTTCGTCACGACCATGAATGGCGTGACACCAGATGACACGCTCAAGGCCGAGCTGCTGGCCCTGGTCAGCAAGGAAATc < 2:21093/150‑1 (MQ=255) cGAAGCCGCGGTGGTCGGCTACCCCCACGACCTCAAGGGCCAAGGCGTCTACGCCTTCGTCACGACCATGAATGGCGTGACACCAGATGACACGCTCAAGGCCGAGCTGCTGGCCCTGGTCAGCAAGGAAATCGGCAGCTTCGCCAAGcc > 2:375686/1‑150 (MQ=255) ccGCGGTGGTCGGCTACCCCCACGACCTCAAGGGCCAAGGCGTCTACGCCTTCGTCACGACCATGAATGGCGTGACACCAGATGACACGCTCAAGGCCGAGCTGCTGGCCCTGGTCAGCAAGGAAATCGGCAGCTTCGCCAAGCCTGAGc < 2:315990/150‑1 (MQ=255) cgGTGGTCGGCTACCCCCACGACCTCAAGGGCCAAGGCGTCTACGCCTTCGTCACGACCATGAATGGCGTGACACCAGATGACACGCTCAAGGCCGAGCTGCTGGCCCTGGTCAGCAAGGAAATCGGCAGCTTCGCCAAGCCTGAGCTGa < 1:202082/150‑1 (MQ=255) ggtCGGCTACCCCCACGACCTCAAGGGCCAAGGCGTCTACGCCTTCGTCACGACCATGAATGGCGTGACACCAGATGACACGCTCAAGGCCGAGCTGCTGGCCCTGGTCAGCAAGGAAATCGGCAGCTTCGCCAAGCCTGAGCTGATTCa < 2:350368/150‑1 (MQ=255) tCGGCTACCCCCACGACCTCAAGGGCCAAGGCGTCTACGCCTTCGTCACGACCATGAATGGCGTGACACCAGATGACACGCTCAAGGCCGAGCTGCTGGCCCTGGTCAGCAAGGAAATCGGCAGCTTCGCCAAGCCTGAGCTGATTCAGt > 2:228121/1‑150 (MQ=255) cGGCTACCCCCACGACCTCAAGGGCCAAGGCGTCTACGCCTTCGTCACGACCATGAATGGCGTGACACCAGATGACACGCTCAAGGCCGAGCTGCTGGCCCTGGTCAGCAAGGAAATCGGCAGCGTCGCCAAGCCTGAGCTGATTCAGTg < 1:268668/150‑1 (MQ=255) ccccACGACCTCAAGGGCCAAGGCGTCTACGCCTTCGTCACGACCATGAATGGCGTGACACCAGATGACACGCTCAAGGCCGAGCTGCTGGCCCTGGTCAGCAAGGAAATCGGCAGCTTCGCCAAGCCTGAGCTGATTCAGTGGGCGCCa > 2:112807/1‑150 (MQ=255) cTCAAGGGCCAAGGCGTCTACGCCTTCGTCACGACCATGAATGGCGTGACACCAGATGACACGCTCAAGGCCGAGCTGCTGGCCCTGGTCAGCAAGGAAATCGGCAGCTTCGCCAAGCCTGAGCTGATTCAGTGGGCGCCAGCGCTAccc < 2:366647/150‑1 (MQ=255) tCAAGGGCCAAGGCGTCTACGCCTTCGTCACGACCATGAATGGCGTGACACCAGATGACACGCTCAAGGCCGAGCTGCTGGCCCTGGTCAGCAAGGAAATCGGCAGCTTCGCCAAGCCTGAGCTGa > 1:392687/1‑126 (MQ=255) tCAAGGGCCAAGGCGTCTACGCCTTCGTCACGACCATGAATGGCGTGACACCAGATGACACGCTCAAGGCCGAGCTGCTGGCCCTGGTCAGCAAGGAAATCGGCAGCTTCGCCAAGCCTGAGCTGa < 2:392687/126‑1 (MQ=255) tCAAGGGCCAAGGCGTCTACGCCTTCGTCACGACCATAAATGGCGTGACACCAGATGACACGCTCAAGGCCGAGCTGCTGGCCCTGGTCAGCAAGGAAATCGGCAGCTTCGCCAAGCCTGAGCTGATTCAGTGGGCGCCAGCGCTACCCa > 1:412203/1‑150 (MQ=255) acgaaGGCGTAGACGCCTTCGTCACGACCATGAATGGCGTGACACCAGATGACACGCTCAAGGCCGAGCTGCTGGCCCTGGTCAGCAAGGAAATCGGCAGCTTCGCCAAGCCTGAGCTGATTCAGTGGGCGCCAGCGCTACCCaa > 1:78409/4‑145 (MQ=255) acgaaGGCGTAGACGCCTTCGTCACGACCATGAATGGCGTGACACCAGATGACACGCTCAAGGCCGAGCTGCTGGCCCTGGTCAGCAAGGAAATCGGCAGCTTCGCCAAGCCTGAGCTGATTCAGTGGGCGCCAGCGCTACCCaa < 2:78409/142‑1 (MQ=255) gCGTCTACGCCTTCGTCACGACCATGAATGGCGTGACACCAGATGACACGCTCAAGGCCGAGCTGCTGGCCCTGGTCAGCAAGGAAATCGGCAGCTTCGCCAAGCCTGAGCTGATTCAGTGGGCGCCAGCGCTACCCAAGACGCGCTCAg < 1:464962/150‑1 (MQ=255) | CTGGATCACCGGGCGCATCGACGATGTGATCAACGTGTCCGGGCACCGCATCGGCACCGCTGAAGTGGAAAGTGCACTGGTGCTGCATGACAGCGTGGCCGAAGCCGCGGTGGTCGGCTACCCCCACGACCTCAAGGGCCAAGGCGTCTACGCCTTCGTCACGACCATGAATGGCGTGACACCAGATGACACGCTCAAGGCCGAGCTGCTGGCCCTGGTCAGCAAGGAAATCGGCAGCTTCGCCAAGCCTGAGCTGATTCAGTGGGCGCCAGCGCTACCCAAGACGCGCTCAG > NC_002947/5346168‑5346460 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |