| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 5,445,697 | G→T | 16.7% | R202L (CGC→CTC) | thiD → | phosphomethylpyrimidine kinase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 5,445,697 | 0 | G | T | 16.7% | 50.3 / 4.0 | 24 | R202L (CGC→CTC) | thiD | phosphomethylpyrimidine kinase |
| Reads supporting (aligned to +/- strand): ref base G (11/9); new base T (2/2); total (13/11) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.59e-01 | |||||||||||
TGCCCGAGGGCACCGCTGACGAATGTGCCGAGAAACTGTTGCCGTTCTGTAGACACCTGCTGATTACCGGCGGTCACGGCGACGAAGACGAAATCCACAACCGCCTGTACACCCAGGACGGCCAGCACTTCACTTGGACTTGCCAGCGCCTGCCGGGCAGCTACCATGGCTCGGGCTGCACCCTGGCCAGCGCCCTGGCCGGCCGCCTGGCGCTCGGCGAGCAGTTGGAAAGCGCCGTGCGCACCGCCCTGGACTACACCTGGCGCACCCTGCGTGACGCCGAGCAACTGGGCA > NC_002947/5445550‑5445843 | tGCCCGAGGGCACCGCTGACGAATTTGCCGAGAAACTGTTGCCGTTCTGTAGACACCTGCTGATTACCGGCGGTCACGGCGACGAAGACGAAATCCACAACCGCCTGTACACCCAGGACGGCCAGCACTTCACTTGGACTTGCCAGCGcc < 2:193956/150‑1 (MQ=255)tGCCCGAGGGCACCGCTGACGAATGTGCCGAGAAACTGTTGCCGTTCTGTAGACACCTGCTGATTACCGGCGGTCACGGCGACGAAGACGAAATCCACAACCGCCTGTACACCCAGGACGGCCAGCACTTCACTTGGACTTGCCAGCGcc > 2:8635/1‑150 (MQ=255) aGGGCACCGCTGACGAATGTGCCGAGAAACTGTTGCCGTTCTGTAGACACCTGCTGATTACCGGCGGTCACGGCGACGAAGACGAAATCCACAACCGCCTGTACACCCAGGACGGCCAGCACTTCACTTGGACTTGCCAGCGCCTGCCgg < 1:305937/150‑1 (MQ=255) aCCGCTGACGAATGTGCCGAGAAACTGTTGCCGTTCTGTAGACACCTGCTGATTACCGGCGGTCACGGCGACGAAGACGAAATCCACAACCGCCTGTACACCCAGGACGGCCAGCACTTCACTTGGACTTGCCAGCGCCTGCCGGGCAGc > 1:308405/1‑150 (MQ=255) cGCTGACGAATGTGCCGAGAAACTGTTGCCGTTCTGTAGACACCTGCTGATTACCGGCGGTCACGGCGACGAAGACGAAATCCACAACCGCCTGTACACCCAGGACGGCCAGCACTTCACTTGGACTTGCCAGCTCCTGCCGGGCAGCTa > 2:328780/1‑150 (MQ=255) cGCTGACGAATGTGCCGAGAAACTGTTGCCGTTCTGTAGACACCTGCTGATTACCGGCGGTCACGGCGACGAAGACGAAATCCACAACCGCCTGTACACCCAGGACGGCCAGCACTTCACTTGGACTTGCCAGCTCCTGCCGGGCAGCTa > 2:328758/1‑150 (MQ=255) aCGAAGGTGCCGAGAAACTGTTGCCGTTCTGTAGACACCTGCTGATTACCGGCGGTCACGGCGACGAAGACGAAATCCACAACCGCCTGTACACCCAGGACGGCCAGCACTTCACTTGGACTTGCCAGCGCCTGCCGGGCAGCTACCATg < 1:178988/150‑1 (MQ=255) ctgctgATTAACGGCGGTCACGGCGACGAAGACGAAATCCACAACCGCCTGTACACCCAGGACGGCCAGCACTTCACTTGGACTTGCCAGCGCCTGCCGGGCAGCTACCATGGCTCGGGCTGCACCCTGGCCAGCGCCCTGGCCGgccgc > 2:212723/1‑150 (MQ=255) gATTACCGGCGGTCACGGCGACGAAGACGAAATCCACAACCGCCTGTACACCCAGGACGGCCAGCACTTCACTTGGACTTGCCAGCGCCTGCCGGGCAGCTACCATGGCTCGGGCTGCACCCTGGCCAGCGCCCTGGCCGGCCGCCTGgc < 1:176735/150‑1 (MQ=255) gcggTCACGGCGACGAAGACGAAATCCACAACCGCCTGTACACCCAGGACGGCCAGCACTTCACTTGGACTTGCCAGCGCCTGCCGGGCAGCTACCATGGCTCGGGCTGCACCCTGGCCAGCGCCCTGGCCGGCCGCCTGGCGCTCGGCg > 1:388315/1‑150 (MQ=255) cggTCACGGCGACGAAGACGAAATCCACAACCGCCTGTACACCCAGGACGGCCAGCACTTCACTTGGACTTGCCAGCGCCTGCCGGGCAGCTACCATGGCTCGGGCTGCACCCTGGCCAGCGCCCTGGCCGGCCGCCTGGCGCTCGGCGa < 1:441032/150‑1 (MQ=255) tCACGGCGACGAAGACGAAATCCACAACCGCCTGTACACCCAGGACGGCCAGCACTTCACTTGGACTTGCCAGCGCCTGCCGGGCAGCTACCATGGCTCGGGCTGCACCCTGGCCAGCGCCCTGGCCGGCCGCCTGGCGCTCGGCGAGCa > 1:274807/1‑150 (MQ=255) agacgaaATCCACAACCGCCTGTACACCCAGGACGGCCAGCACTTCACTTGGACTTGCCAGCGCCTGCCGGGCAGCTACCATGGCTCGGGCTGCACCCTGGCCAGCGCCCTggccg > 1:401549/1‑116 (MQ=255) agacgaaATCCACAACCGCCTGTACACCCAGGACGGCCAGCACTTCACTTGGACTTGCCAGCGCCTGCCGGGCAGCTACCATGGCTCGGGCTGCACCCTGGCCAGCGCCCTggccg < 2:401549/116‑1 (MQ=255) gacgaaATCCACAACCGCCTGTACACCCAGGACGGCCAGCACTTCACTTGGACTTGCCAGCGCCTGCCGGGCAGCTACCATGGCTCGGGCTGCACCCTGGCCAGCGCCCTGGCCGGCCGCCTGGCGCTCGGCGAGCAGTTGGAAAGCGcc < 2:308405/150‑1 (MQ=255) acgaaATCCACAACCGCCTGTACACCCAGGACGGCCAGCACTTCACGTGGACTTGCCAGCGCCTGCCGGGCGGCTACCATGGGCCGGCGATCACCCTGGTCAGCTCCCTGGCCGGCCGCCTGGCGCTCGGCGAGCAGTTGGAAAGCGCCg > 1:36199/1‑150 (MQ=255) ccTGTACACCCAGGACGGCCAGCACTTCACTTGGACTTGCCAGCGCCTGCCGGGCAGCTACCATGGCTCGGGCTGCACCCTGGCCAGCGCCCTGGCCGGCCGCCTGGCGCTCGGCGAGCAGTTGGAAAGCGCCGTGCGCACCGCCCTGGa > 1:96521/1‑150 (MQ=255) acCCAGGACGGCCAGCACTTCACTTGGACTTGCCAGCGCCTGCCGGGCAGCTACCATGGCTCGGGCTGCACCCTGTCCAGCGCCCTGGCCGGCCGCCTGGCGCTCGGCGAGCAGTTGGAAAGCGCCGTGCGCACCGCCCTGGACTACAcc > 2:503988/1‑150 (MQ=255) aGCACTTCACTTGGACTTGCCAGCTCCTGCCGGGCAGCTACCATGGCTCGGGCTGCACCCTGGCCAGCGCCCTGGCCGGCCGCCTGGCGCTCGGCGAGCAGTTGGAAAGCGCCGTGCGCACCGCCCTGGACTACACCTGGCGCACCCTGc < 1:328780/150‑1 (MQ=255) aGCACTTCACTTGGACTTGCCAGCTCCTGCCGGGCAGCTACCATGGCTCGGGCTGCACCCTGGCCAGCGCCCTGGCCGGCCGCCTGGCGCTCGGCGAGCAGTTGGAAAGCGCCGTGCGCACCGCCCTGGACTACACCTGGCGCACCCTGc < 1:328758/150‑1 (MQ=255) tcccttGGACTTGCCAGCGCCTGCCGGGCAGCTACCATGGCTCGGGCTGCACCCTGGCCAGCGCCCTGGCCGGCCGCCTGGCGCTCGGCGAGCAGTTg < 2:107062/95‑1 (MQ=255) tcacttGGACTTGCCAGCGCCTGCCGGGCAGCTACCATGGCTCGGGCTGCACCCTGGCCAGCGCCCTGGCCGGCCGCCTGGCGCTCGGCGAGCAGTTg > 1:107062/1‑98 (MQ=255) ttGGACTTGCCAGCGCCTGCCGGGCAGCTACCATGGCTCGGGCTGCACCCTGGCCAGCGCCCTGGCCGGCCGCCTGGCGCTCGGCGAGCAGTTGGAAAGCGCCGTGCGCACCGCCCTGGACTACACCTGGCGCACCCTGCGTGACGCCGa < 1:8635/150‑1 (MQ=255) aGCGCCTGCCGGGCAGCTACCATGGCTCGGGCTGCACCCTGGCCAGCGCCCTGGCCGGCCGCCTGGCGCTCGGCGAGCAGTTGGAAAGCGCCGTGCGCACCGCCCTGGACTACACCTGGCGCACCCTGCGTGACGCCGAGCAACTGGGCa > 1:29792/1‑150 (MQ=255) | TGCCCGAGGGCACCGCTGACGAATGTGCCGAGAAACTGTTGCCGTTCTGTAGACACCTGCTGATTACCGGCGGTCACGGCGACGAAGACGAAATCCACAACCGCCTGTACACCCAGGACGGCCAGCACTTCACTTGGACTTGCCAGCGCCTGCCGGGCAGCTACCATGGCTCGGGCTGCACCCTGGCCAGCGCCCTGGCCGGCCGCCTGGCGCTCGGCGAGCAGTTGGAAAGCGCCGTGCGCACCGCCCTGGACTACACCTGGCGCACCCTGCGTGACGCCGAGCAACTGGGCA > NC_002947/5445550‑5445843 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |