| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 5,550,720 | T→A | 21.1% | L305Q (CTG→CAG) | PP_4881 → | iron ABC transporter substrate‑binding protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 5,550,720 | 0 | T | A | 21.1% | 31.9 / 6.1 | 19 | L305Q (CTG→CAG) | PP_4881 | iron ABC transporter substrate‑binding protein |
| Reads supporting (aligned to +/- strand): ref base T (7/8); new base A (2/2); total (9/10) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.64e-01 | |||||||||||
CAGGCGTGGTCAAGGCCAGCAAACACCCGAAAGAAGCCCAGGCCCTGCTCAACTGGATGGCCAGCGAAGAAGGCCAGCGCGTGATCACCCAGACCACCGCCGAGTACCCACTGCACAAGGGCATGGTTTCTGACCGCGGCCTGAAACCGTTCGACGAGCTGCGCCCGCCGAAGATCTCGCCAGCTGACCTGGGCAATGCCGAGGAAGCCATCGAACTTGAGCGTGAGGTCGGCCTGCTCTGATGACTGCCGCCCTGTCCGAACCGGTGCCGG > NC_002947/5550579‑5550850 | cAGGCGTGGTCAAGGCCAGCAAACACCCGAAAGAAGCCCAGGCCCTGCTCAACTGGATGGCCAGCGAAGAAGGCCAGCGCGTGATCACCCAGACCACCGCCGAGTACCCACTGCACAAGGGCATGGTTTCTGACCGCGGCCTGAAACCGt < 1:227801/150‑1 (MQ=255) aGGCGTGGTCAAGGCCAGCAAACACCCGAAAGAAGCCCAGGCCCTGCTCAACTGGATGGCCAGCGAAGAAGGCCAGCGCGTGATCACCCAGACCACCGCCGAGTACCCACTGCACAAGGGCATGGTTTCAGGCCGCGGTCAGAAAccatg < 1:77219/150‑4 (MQ=255) ccGAAAGAAGCCCAGGCCCTGCTCAACTGGATGGCCAGCGAAGAAGGCCAGCGCGTGATCACCCAGACCACCGCCGAGTACCCACTGCACAAGGGCATGGTTTCTGACCGCGGCCTGAAACCGTTCGACGAGCTGCGCCCGCCGAAGAtc > 1:156034/1‑150 (MQ=255) cGAAAGAAGCCCAGGCCCTGCTCAACTGTATGGCCAGCGAAGAAGGCCAGCGCGTGATCACCCAGACCACCGCCGAGTACCCACTGCACAAGGGCATGGTTTCAGGCCGCGGTCAGAAAccatgcc < 2:364619/126‑6 (MQ=255) cGAAAGAAGCCCAGGCCCTGCTCAACTGGATGGCCAGCGAAGAAGGCCAGCGCGTGATCACCCAGACCACCGCCGAGTACCCACTGCACAAGGGCATGGTTTCAGGCCGCGGTCAGAAAccatgcc > 1:364619/1‑121 (MQ=255) ccTGCTCAACTGGATGGCCAGCGAAGAAGGCCAGCGCGTGATCACCCAGACCACCGCCGAGTACCCTCTGCACAAGGGCATGGTTTCTGACCGCGGCCTGAAACCGTTCGACGAGCTGCGCCCGCCGAAGATCTCGCCAGCTGACCTggg < 2:270878/150‑1 (MQ=255) aaCTGGATGGCCAGCGAAGAAGGCCAGCGCGTGATCACCCAGACCACCGCCGAGTACCCACTGCACAAGGGCATGGTTTCTGACCGCGGCCTGAAACCGTTCGACGAGCTGCGCCCGCCGAAGATCTCGCCAGCTGACCTGGGCAATGcc < 2:425308/150‑1 (MQ=255) aaCTGGATGGCCAGCGAAGAAGGCCAGCGCGTGATCACCCAGACCACCGCCGAGTACCCACTGCACAAGGGCATGGTTTCTGACCGCGGCCTGAAACCGTTCGACGAGCTGCGCCCGCCGAAGATCTAGCCAGCTGACCTGGGCAATGcc > 2:237680/1‑150 (MQ=255) cTGGATGGCCAGCGAAGAAGGCCAGCGCGTGATCACCCAGACCACCGCCGAGTACCCACTGCACAAGGGCATGGTTTCTGACCGCGGCCTGATACCGTTCGACGAGCTGCGCCCGCCGAAGATCTCGCCAGCTGACCTGGGCAATGCCGa < 1:183174/150‑1 (MQ=255) cTGGATGGCCAGCGAAGAAGGCCAGCGCGTGATCACCCAGACCACCGCCGAGTACCCACTGCACAAGGGCATGGTTTCTGACCGCGGCCTGAAACCGTTCGACGAGCTGCGCCCGCCGAAGATCTCGCCAGCTGACCTGGGCAATGCCGa < 1:368875/150‑1 (MQ=255) ggATGGCCAGCGAAGAAGGCCAGCGCGTGATCACCCAGACCACCGCCGAGTACCCACTGCACAAGGGCATGGTTTCTGACCGCGGCCTGAAACCGTTCGACGAGCTGCGCCCGCCGAAGATCTCGCCAGCTGACCTGGGCAATGCCGAgg > 2:446478/1‑150 (MQ=255) cAGCGAAGAAGGCCAGCGCGTGATCACCCAGACCACCGCCGAGTACCCACTGCACAAGGGCATGGTTTCTGACCGCGGCCTGAAACCGTTCGACGAGCTGCGCCCGCCGAAGATCTCGCCAGCTGACCTGGGCAATGCCGAGGAAGCCAt > 2:289625/1‑150 (MQ=255) gATCACCCAGACCACCGCCGAGTACCCACTGCACAAGGGCATGGTTTCTGACCGCGGCCTGAAACCGTTCGACGAGCTGCGCCCGCCGAAGATCTCGCCAGCTGACCTGGGCAATGCCGAGGAAGCCATCGAACTTGAGCGTGAGGTCgg < 2:156034/150‑1 (MQ=255) aTCACCCAGACCACCGCCGAGTACCCACTGCACAAGGGCATGGTTTCTGACCGCGGCCTGAAACCGTTCGACGAGCTGCGCCCGCCGAAGATCTCGCCAGCTGACCTGGGCAATGCCGAGGAAGCCATCGAACTTGAGCGTGACGTCGGc < 2:429431/150‑1 (MQ=255) aCCCAGACCACCGCCGAGTACCCACTGCACAAGGGCATGGTTTCTGACCGCGGCCTGAAACCGTTCGACGAGCTGCGCCCGCCGAAGATCTCGCCAGCTGACCTGGGCAATGCCGAGGAAGCCATCGAACTTGAGCGTGAGGTCGGCCTg > 2:200919/1‑150 (MQ=255) cACTGCACAAGGGCATGGTTTCTGACCGCGGCCTTAAGCCGTGCGACGAGCTGCGCCCGCCGAACTTGAGACCATCTGACCTGGGCAATGCCGAGGAAGCCATCGAACTTGAGCGTGAGGTCGGCCTGCTCTGATGACTGCCGCCCTGTc < 1:215941/150‑1 (MQ=255) cACTGCACAAGGGCATGGTTTCTGACCGCGGCCTGAAACCGTTCGACGCCGGGTTCCCGCCGAAGATCTCGCCAGCTGACCTGGGCAATGCCGAGGAAGCCATCGAACTTGAGCGTGAGGTCGGCCTGCTCTGATGACTGCCGCCCTGTc > 1:418089/1‑150 (MQ=255) aacggTTTCAGGCCGCGGTCAGAAACCGTTCGACGAGCTGCGCCCGCCGAAGATCTCGCCAGCTGACCTGGGCAATGCCGAGGAAGCCATCGAACTTGAGCGTGAGGTCGGCCTGCTCTGATGACTGCCGCCCTGTCCGAACCGGTGCCg > 2:228501/4‑150 (MQ=255) aTGGTTTCTGACCGCGGCCTGAAACCGTTCGACGAGCTGCGCCCGCCGAAGATCTCGCCAGCTGACCTGGGCAATGCCGAGGAAGCCATCGAACTTGAGCGTGAGGTCGGCCTGCTCTGATGACTGCCGCCCTGTCCGAACCGGTGCCgg > 2:74665/1‑150 (MQ=255) | CAGGCGTGGTCAAGGCCAGCAAACACCCGAAAGAAGCCCAGGCCCTGCTCAACTGGATGGCCAGCGAAGAAGGCCAGCGCGTGATCACCCAGACCACCGCCGAGTACCCACTGCACAAGGGCATGGTTTCTGACCGCGGCCTGAAACCGTTCGACGAGCTGCGCCCGCCGAAGATCTCGCCAGCTGACCTGGGCAATGCCGAGGAAGCCATCGAACTTGAGCGTGAGGTCGGCCTGCTCTGATGACTGCCGCCCTGTCCGAACCGGTGCCGG > NC_002947/5550579‑5550850 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |