| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 5,685,956 | G→C | 17.3% | P331P (CCC→CCG) ‡ | PP_4988 ← | signal transduction histidine kinase CheA/CheY‑like receiver |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 5,685,956 | 0 | G | C | 17.3% | 69.9 / 9.8 | 29 | P331P (CCC→CCG) ‡ | PP_4988 | signal transduction histidine kinase CheA/CheY‑like receiver |
| Reads supporting (aligned to +/- strand): ref base G (12/12); new base C (2/3); total (14/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.79e-01 | |||||||||||
GCGTCAGCTCATCAATCGGCGGCTCTTCCTCCAGGTGCTGCAACCAACGCTGCACATAGCTTGCGCAGCCGACAAACAGCTCGGCCACGGCCGGCAGCATCAACATGGCCAATGCCCCGCGGATCTGCTGCAACAGCCCCGGCAAGGGCTGCAGGCGTTGCCGGGGCCAGTCGGACTCCAGGTAATCGCCGATCAGATCCTTGGCCTGCTGCAACACGTTCAGCGATTCATTCAGCACCAGCTGGCGTATCTCGGCCAGGTCCGAGCCCGGCAGGCTGCCTTGGCCGTTT > NC_002947/5685811‑5686100 | gCGTCAGCTCATCAATCGGCGGCTCTTCCTCCAGGTGCTGCAACCAACGCTGCACATAGCTTGCGCAGCCGACAAACAGCTCGGCCACGGCCGGCAGCATCAACATGGCCAATGCCCCGCGGATCTGCTGCAACAGCCCCGGCAAGGGCt < 2:261455/150‑1 (MQ=255) gCTCATCAATCGGCGGCTCTTCCTCCAGGTGCTGCAACCAACGCTGCACATAGCTTGCGCAGCCGACAAACAGCTCGGCCACGGCCGGCAGCATCAACATGGCCAATGCCCCGCGGATCTGCTGCAACAGCCCCGGCAACGCCTGCAgcc > 2:506993/1‑148 (MQ=255) atcaATCGGCGGCTCTTCCTCCAGGTGCTGCAACCAACGCTGCACATAGCTTGCGCAGCCGATAAACAGCTCGGCCACGGCCGGCAGCATCAACATGGCCAATGCCCCGCGGATCTGCTGCAACAGCCCCGGCAAGGGCTGCAGGCGTTg > 2:319288/1‑150 (MQ=255) tCGGCGGCTCTTCCTCCAGGTGCTGCAACCAACGCTGCACATAGCTTGCGCAGCCGACAAACAGCTCGGCCACGGCCGGCAGCATCAACATGGCCAATGCCCCGCGGATCTGCTGCAACAGCCCCGGCAACGCCTGCAGCCCTTGCCggg < 1:371776/150‑1 (MQ=255) ggcggcTCTTCCTCCAGGTGCTGCAACCAACGCTGCACATAGCTTGCGCAGCCGACAAACAGCTCGGCCACGGCCGGCAGCATCAACATGGCCAATGCCCCGCGGATCTGCTGCAACAGCCCCGGCAACGCCTGCAGCCCTTGCCGgcgc < 1:506993/150‑1 (MQ=255) ctctTCCTCCAGGTGCTGCAACCAACGCTGCACATAGCTTGCGCAGCCGACAAACAGCTCGGCCACGGCCGGCAGCATCAACATGGCCAATGCCCCGCGGATCTGCTGCAACAGCCCCGGCAAGGGCTGCAGGCGTTGCCGGGGCCAGTc < 2:426328/150‑1 (MQ=255) ggTGCTGCAACCAACGCTGCACATAGCTTGCGCAGCCGACAAACAGCTCGGCCACGGCCGGCAGCATCAACATGGCCAATGCCCCGCGGATCTGCTGCAACAGCCCCGGCAAGGGCTGCAGGCGTTGCCGGGGCCAGTCGGACTCCAGGt > 2:182214/1‑150 (MQ=255) ctgcAACCAACGCTGCACATAGCTTGCGCAGCCGACAAACAGCTCGGCCACGGCCGGCAGCATCAACATGGCCAATGCCCCGCGGATCTGCTGCAACAGCCCCGGCAAGGGCTGCAGGCGTTGCCGGGGCCAGTCGGACTCCAGGTAATc > 2:475465/1‑150 (MQ=255) ctgcAACCAACGCTGCACATAGCTTGCGCAGCCGACAAACAGCTCGGCCACGACCGGAAGCATCAACATGGCCAATGCCCCGCGGATCTGCTGCAACAGCCCCGGCAAGGGCTGCAGGCGTTGCCGGGGCCAGTCGGACTCCAGGTAATc < 1:449452/150‑1 (MQ=255) accaacGCTGCACATAGCTTGCGCAGCCGATAAACAGCTCGGCCACGGCCGGCAGCATCAACATGGCCAATGCCCCGCGGATCTGCTGCAACAGCCCCGGCAAGGGCTGCAGGCGTTGCCGGGGCCAGTCGGACTCCAGGTAATCGCCGa < 1:319288/150‑1 (MQ=255) aTAGCTTGCGCAGCCGACAAACAGCTCGGCCACGGCCGGCAGCATCAACATGGCCAATGCCCCGCGGATCTGCTGCAACAGCCCCGGCAAGGGCTGCAGGCGTTGCCGGGGCCAGTCGGACTCCAGGTAATCGCCGATCAGATCCTTGGc > 1:304103/1‑150 (MQ=255) aCAGCTCGGCCACGGCCGGCAGCATCAACATGGCCAATGCCCCGCGGATCTGCTGCAACAGCCCCGGCAAGGGCTGCAGGCGTTGCCGGGGCCAGTCGGACTCCAGGTAATCGCCGATCAGATCCTTGGCCTGCTGCAACACGTTCa < 1:117971/147‑1 (MQ=255) aCAGCTCGGCCACGGCCGGCAGCATCAACATGGCCAATGCCCCGCGGATCTGCTGCAACAGCCCCGGCAAGGGCTGCAGGCGTTGCCGGGGCCAGTCGGACTCCAGGTAATCGCCGATCAGATCCTTGGCCTGCTGCAACACGTTCa > 2:117971/1‑147 (MQ=255) cGGCCACGGCCGGCAGCATCAACATGGCCAATGCCCCGCGGATCTGCTGCAACAGCCCCGGCAAGGGCTGCAGGCGTTGCCGGGGCCAGTCGGACTCCAGGTAATCGCCGATCAGATCCTTGGCctgct < 1:481988/129‑1 (MQ=255) cGGCCACGGCCGGCAGCATCAACATGGCCAATGCCCCGCGGATCTGCTGCAACAGCCCCGGCAAGGGCTGCAGGCGTTGCCGGGGCCAGTCGGACTCCAGGTAATCGCCGATCAGATCCTTGGCctgct > 2:481988/1‑129 (MQ=255) cggcAGCATCAACATGGCCAATGCCCCGCGGATCTGCTGCAACAGCCCCGGCAAGGGCTGCAGGCGTTGCCGGGGCCAGTCGGACTCCAGGTAATCGCCGATCAGATCCTTGGCCTGCTGCAACACGTTCAGCGATTCATTCAGCACCAg > 2:428656/1‑150 (MQ=255) aTGGCCAATGCCCCGCGGATCTGCTGCAACAGCCCCGGCAAGGGCTGCAGGCGTTGCCGGGGCCAGTCGGACTCCAGGTAATCGCCGATCAGATCCTTGGCCTGCTGCAACACGTTCAGCGATTCATTAAGCACCAGCTGGCGTATCTCg < 1:50392/150‑1 (MQ=255) aaTGCCCCGCGGATCTGCTGCAACAGCCCCGGCAAGGGCTGCAGGCGTTGCCGGGGCCAGTCGGACTCCAGGTAATCGCCGATCAGATCCTTGGCCTGCTGCAACACGTTCAGCGATTCATTCAGCACCAGCTGGCGTATCTCGGCCAgg > 1:414765/1‑150 (MQ=255) cccGCGGATCTGCTGCAACAGCCCCGGCAAGGGCTGCAGGCGTTGCCGGGGCCAGTCGGACTCCAGGTAATCGCCGATCAGATCCTTGGCCTGCTGCAACACGTTCAGCGATTCATTCAGCACCAGCTGGCGTATCTCGGCCAGGTCCGa > 2:281821/1‑150 (MQ=255) gcgGATCTGCTGCAACAGCCCCGGAAAGGGCTGCAGGCGTTGCCGGGGCCAGTCGGACTCCAGGTAATCGCCGATCAGATCCTTGGCCTGCTGCAACACGTTCAGCGATTCATTCAGCACCAGCTGGCGTATCTCGGCCAGGTCCGAGcc > 1:196876/1‑150 (MQ=255) tCTGCTGCAACAGCCCCGGCAAGGGCTGCAGGCGTTGCCGGGGCCAGTCGGACTCCa < 1:503467/57‑1 (MQ=255) tCTGCTGCAACAGCCCCGGCAAGGGCTGCAGGCGTTGCCGGGGCCAGTCGGACTCCa > 2:503467/1‑57 (MQ=255) tCTGCTGCAACAGCCCCGGCAAGGGCTGCAGGCGTTGCCGGGGCCAGTCGGACTCCAGGTAATCGCCGATCAGATCCTTGGCCTGCTGCAACACGTTCAGCGATTCATTCAGCACCAGCTGGCGTATCTCGGCCAGGTCCGAGCCCGGCa < 1:182214/150‑1 (MQ=255) ctgcAACAGCCCCGGCAAGGGCTGCAGGCGTTGCCGGGGCCAGTCGGACTCCAGGTAATCGCCGATCAGATCCTTGGCCTGCTGCAACACGTTCAGCGATTCATTCAGCACCAGCTGGCGTATCTCGGCCAGGTCCGAGCCCGGCAGGCt < 1:480449/150‑1 (MQ=255) tgcAACAGCCCCGGCAAGGGCTGCAGGCGTTGCCGGGGCCAGTCGGACTCCAGGTAATCGCCGATCAGATCCtt < 1:230766/74‑1 (MQ=255) tgcAACAGCCCCGGCAAGGGCTGCAGGCGTTGCCGGGGCCAGTCGGACTCCAGGTAATCGCCGATCAGATCCtt > 2:230766/1‑74 (MQ=255) cccGGCAACGCCTGCAGCCCTTGCCGGGGCCAGTCGGACTCCAGGTAATCGCCGATCAGATCCTTGGCCTGCTGCAACACGTTCAGCGATTCATTCAGCACCAGCTgg < 2:255952/108‑1 (MQ=38) cccGGCAACGCCTGCAGCCCTTGCCGGGGCCAGTCGGACTCCAGGTAATCGCCGATCAGATCCTTGGCCTGCTGCAACACGTTCAGCGATTCATTCAGCACCAGCTgg > 1:255952/1‑108 (MQ=38) ggCAAGGGCTGCAGGCGTTGCCGGGGCCAGTCGGACTCCAGGTAATCGCCGATCAGATCCTTGGCCTGCTGCAACACGTTCAGCGATTCATTCAGCACCAGCTGGCGTATCTCGGCCAGGTCCGAGCCCGGCAGGCTGCCTTGGCCGttt < 2:304103/150‑1 (MQ=255) | GCGTCAGCTCATCAATCGGCGGCTCTTCCTCCAGGTGCTGCAACCAACGCTGCACATAGCTTGCGCAGCCGACAAACAGCTCGGCCACGGCCGGCAGCATCAACATGGCCAATGCCCCGCGGATCTGCTGCAACAGCCCCGGCAAGGGCTGCAGGCGTTGCCGGGGCCAGTCGGACTCCAGGTAATCGCCGATCAGATCCTTGGCCTGCTGCAACACGTTCAGCGATTCATTCAGCACCAGCTGGCGTATCTCGGCCAGGTCCGAGCCCGGCAGGCTGCCTTGGCCGTTT > NC_002947/5685811‑5686100 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |