Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,829,231 C→G 14.8% A272P (GCA→CCA)  ftsX ← cell division protein FtsX

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,829,2310CG14.8% 69.9 / 3.3 26A272P (GCA→CCA) ftsXcell division protein FtsX
Reads supporting (aligned to +/- strand):  ref base C (12/10);  new base G (2/2);  total (14/12)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CTGCTCCGATCAAGAGCGACAGACCATCGGAAGCCGGAACGCCGCCCAAGGCGAAGTCACTGCCATACAGCCCGGAAAGCCCTACCACCGCCTCGTTCAGCCAGTTCAGGCCA‑AACGCCAGGATGCCCCACGCCAGCAGGCCTGCGCCCAGGCCATACAGGGCGCCCATGTACAGGAAAGGCCGGCGCACGTAGGCGTCGGTGCCGCCGACCAGCTTGATCACTTCGATCTCGATACGACGGTTTTCAATGTGTAGACGAATTGTGTTACCGATTACTAACAGCAGGGCAGA  >  NC_002947/5829087‑5829378
                                                                                                                                                 |                                                                                                                                                   
cTGCTCCGATCAAGAGCGACAGACCATCGGAAGCCGGAACGCCGCCCAAGGCGAAGTCACTGCCATACAGCCCGGAAAGCCCTACCACCGCCTCGTTCAGCCAGTTCAGGCCA‑AACGCCAGGATGCCCCACGCCAGCAGGCCTGCGCCCa                                                                                                                                                <  2:423808/150‑1 (MQ=255)
cTGCTCCGATCAAGAGCGACAGACCATCGGAAGCCGGAACGCCGCCCAAGGCGAAGTCACTGCCATACAGCCCGGAAAGCCCTACCACCGCCTCGTTCAGCCAGTTCAGGCCA‑AACGCCAGGATGCCCCACGCCAGCAGGCCTGCGCCCa                                                                                                                                                <  2:271231/150‑1 (MQ=255)
      cGATCAAGAGCGACAGACCATCGGAAGCCGGAACGCCGCCCAAGGCGAAGTCACTGCCATACAGCCCGGAAAGCCCTACCACCGCCTCGTTCAGCCAGTTCAGGCCA‑ACGCCCAGGATGCCCCACGCCAGCAGGCCTGGGCGCAGGcctg                                                                                                                                          >  1:133752/1‑148 (MQ=255)
       gATCAAGAGCGACAGACCATCGGCCGCCGAGACGCCGCCCAAGGCGAAGGCACTGCCATACAGCCCGGAAAGCCCTACCACCGCCTCGTTCAGCCAGTTCAGGCCA‑AACGCCAGGATGCCCCACGCCAGCAGGCCTGGGCGCAGGcctgc                                                                                                                                         <  2:133752/150‑4 (MQ=255)
                         aTCGGAAGCCGGAACGCCGCCCAAGGCGAAGTCACTGCCATACAGCCCGGAAAGCCCTACCGCCGCCTCGTTCAGCCAGTTCAGGCCA‑AACGCCAGGATGCCCCACGCCAGCAGGCCTGCGCCCAGGCCATACAGGgcgc                                                                                                                                 <  2:422838/140‑1 (MQ=255)
                         aTCGGAAGCCGGAACGCCGCCCAAGGCGAAGTCACTGCCATACAGCCCGGAAAGCCCTACCACCGCCTCGTTCAGCCAGTTCAGGCCA‑AACGCCAGGATGCCCCACGCCAGCAGGCCTGCGCCCAGGCCATACAGGgcgc                                                                                                                                 >  1:422838/1‑140 (MQ=255)
                            ggAAGCCGGAACGCCGCCCAAGGCGAAGTCACTGCCATACAGCCCGGAAAGCCCTACCACCGCCTCGTTCAGCCAGTTCAGGCCA‑AACGCCAGGATGCCCCACGCCAGCAGGCCTGCGCCCAGGCCATACAGGGCGCCCATGTACAGGaa                                                                                                                    <  2:228614/150‑1 (MQ=255)
                                                    gAAGTCACTGCCATACGGCCCGGAAAGCCCTACCACCGCCTCGTTCAGCCAGTTCAGGCCA‑AACGCCAGGATGCCCCACGCCAGCAGGCCTGCGCCCAGGCCATACAGGGCGCCCATGTACAGGAAAGGCCGGCGCACGTAGGCGTCGGt                                                                                            <  1:148567/150‑1 (MQ=255)
                                                    gAAGTCACTGCCATACAGCCCGGAAAGCCCTACCACCGCCTCGTTCAGCCAGTTCAGGCCA‑AACGCCAGGATGCCCCACGCCAGCAGGCCTGCGCCCAGGCCATACAGGGCGCCCATGTACAGGAAAGGCCGGCGCACGTAGGCGTCGGt                                                                                            >  1:292580/1‑150 (MQ=255)
                                                            tGCCATACAGCCCGGAAAGCCCTACCACCGCCTCGTTCAGCCAGTTCAGGCCA‑AACGCCAGGATGCCCCACGCCAGCAGGCCTGCGCCCAGGCCATACAGGGCGCTCATGTACAGGAAAGGCCGGCGCACGTAGGCGTCGGTGCCGCCGa                                                                                    >  2:396729/1‑150 (MQ=255)
                                                                   cAGCCCGGAAAGCCCTACCACCGCCTCGTTCAGCGA‑CTCTTCCGATATCTCGAGTAGGCCCCACGCCAGCAGGCCGCGGCCCAGGCCATACAGGGCGCCCATGTACAGGAAAGGCCGGCGCACGTAGGCGTCGGTGCCGCCGACCAGCtt                                                                             <  1:494735/150‑1 (MQ=255)
                                                                          gAAAGCCCTACCACCGCCTCGTTCAGCCAGTTCAAGAAA‑AACTCCATGATGCCCCACGCCAGCAGGCCTTCGCCCAGGAACTATACGGCCCCCATGTACAGGAAAGGCCGGCGCACGTAGGCGTCGGTGCCGCCGACCAGCTTGATCACt                                                                      >  1:369167/1‑150 (MQ=255)
                                                                               cccTACCACCGCCTCGTTCAGCCAGTTCAGGCCA‑AACGCCAGGATGCCCCACGCCAGCAGGCCTgc                                                                                                                                                     >  1:181090/1‑66 (MQ=255)
                                                                               cccTACCACCGCCTCGTTCAGCCAGTTCAGGCCA‑AACGCCAGGATGCCCCACGCCAGCAGGCCTgc                                                                                                                                                     <  2:181090/66‑1 (MQ=255)
                                                                                               atCAGCCAGTTCAGGCCA‑AACGCCAGGATGCCCCACGCCAGCAGGCCTGCGCCCAGGCCATACAGGGCGCCCATGTACAGGAAAGGCCGGCGCACGTAGGCGTCGGTGCCGCCGACCAGCTTGATCACTTCGATCTCGATACGACGGttt                                                 >  2:350092/2‑150 (MQ=255)
                                                                                                    ccAGTTCAGGCCA‑AACGCCAGGATGCCCCACGCCAGCAGGCCTGCGCCCAGGCCATACAGGGCGCCCATGTACAGGAAAGGCCGGCGCACGTAGGCGTCGGTGCCGCCGACCAGCTTGATCACTTCGATCTCGATACGACGGTTTTCAAt                                            >  2:148168/1‑150 (MQ=255)
                                                                                                     cAGTTCAGGCCA‑AACGCCAGGATGCCCCACGCCAGCAGGCCTGCGCCCAGGCCATACAGGGCGCCCATGTACAGGAAAGGCCGGCGCACGTAGGCGTCGGTGCCGCCGACCAGCTTGATCACTTCGATCTCGATACGACGGTTTTCAAtg                                           >  1:308546/1‑150 (MQ=255)
                                                                                                      aGTTCAGGCCA‑AACGCCAGGATGCCCCACGCCAGCAGGCCTGCGCCCAGGCCATACAGGGCGCCCATGTACAGGAAAGGCCGGCGCACGTAGGCGTCGGTGCCGCCGACCAGCTTGATCACTTCGATCTCGATACGACGGTTTTCAAtgt                                          <  1:1600/150‑1 (MQ=255)
                                                                                                           aGGCCA‑AACGCCAGGATGCCCCACGCCAGCAGGCCTGCGCCCAGGCCATACAGGGCGCTCATGTACAGGAAAGGCCGGCGCACGTAGGCGTCGGTGCCGCCGACCAGCTTGATCACTTCGATCTCGATACGACGGTTTTCAATGTGTAGa                                     <  1:396729/150‑1 (MQ=255)
                                                                                                           aGGCCA‑AACGCCAGGATGCCCCACGCCAGCAGGCCTGCGCCCAGGCCATACAGGGCGCCCATGTACAGGAAAGGCCGGCGCACGTAGGCGTCGGTGCCGCCGACCAGCTTGATCACTTCGATCTCGATACGACGGTTTTCAATGTGTAGa                                     <  2:308546/150‑1 (MQ=255)
                                                                                                                       cAGGATGCCCCACGCCAGCAGGCCTGCGCCCAGGCCATACAGGGCGCCCATGTACAGGAAAGGCCGGCGCACGGAGGCGTCGGTGCCGCCGACCAGCTTGATCACTTCGATCTCGATACGACGGTTTTCAATGTGTAGACGAATTGTGtt                          >  1:269406/1‑150 (MQ=255)
                                                                                                                        aGGATGCCCCACGCCAGCAGGCCTGCGCCCAGGCCATACAGGGCGCCCATGTACAGGAAAGGCCGGCGCACGTAGGCGTCGGTGCCGCCGACCAGCTTGATCACTTCGATCTCGATACGACGGTTTTCAATGTTTAGACGAATTGTGTTa                         >  2:245053/1‑150 (MQ=255)
                                                                                                                        aGGATGCCCCACGCCAGCAGGCCTGCGCCCAGGCCATACAGGGCGCCCATGTACAGGAAAGGCCGGCGCACGTAGGCGTCGGTGCCGCCGACCAGCTTGATCACTTCGATCTCGATACGACGGTTTTCAATGTGTAGACGAATTGTGTTa                         <  1:245053/150‑1 (MQ=255)
                                                                                                                         ggATGCCCCACGCCAGCAGGCCTGCGCCCAGGCCATACAGGGCGCCCATGTACAGGAAAGGCCGGCGCACGTAGGCGTCGGTGCCGCCGACCAGCTTGATCACTTCGATCTCGATACGACGGTTTTCAATGTGTAGACGAATTGTGTTAc                        <  2:292580/150‑1 (MQ=255)
                                                                                                                                ccACGCCAGCAGGCCTGCGCCCAGGCCATACAGGGCGCCCATGTACAGGAAAGGCCGGCGCACGTAGGCGTCGGTGCCGCCGACCAGCTTGATCACTTCGATCTCGATACGACGGTTTTCAATGTGTAGACGAATTGTGTTACCGATTAc                 >  2:243233/1‑150 (MQ=255)
                                                                                                                                ccACGCCAGCAGGCCTGCGCCCAGGCCATACAGGGCGCCCATGTACAGGAAAGGCCGGCGCACGTAGCCGTCGGTGCCGCCGACCAGCTTGATCACTTCGATCTCGATACGACGGTTTTCAATGTGTAGACGAATTGTGTTACCGATTAc                 >  1:184470/1‑150 (MQ=255)
                                                                                                                                     ctgtatggCCTGGGCGCAGGCCATACAGGGCGCCCATGTACAGGAAAGGCCGGCGCACGTAGGCGTCGGTGCAGCCGACCAGCTTGATCACTTCGATCTCGATACGACGGTTTTCCATGTGTAGACGAATTGGGTTACCGATTACTAAca            >  2:190561/7‑150 (MQ=255)
                                                                                                                                               tGCGCCCAGGCCATACAGGGCGCCCATGTACAGGAAAGGCCGGCGCACGTAGGCGTCGGTGCCGCCGACCAGCTTGATCACTTCGATCTCGATACGACGGTTTTCAATGTGTAGACGAATTGTGTTACCGATTACTAACAGCAGGGCAGa  >  2:340150/1‑150 (MQ=255)
                                                                                                                                                 |                                                                                                                                                   
CTGCTCCGATCAAGAGCGACAGACCATCGGAAGCCGGAACGCCGCCCAAGGCGAAGTCACTGCCATACAGCCCGGAAAGCCCTACCACCGCCTCGTTCAGCCAGTTCAGGCCA‑AACGCCAGGATGCCCCACGCCAGCAGGCCTGCGCCCAGGCCATACAGGGCGCCCATGTACAGGAAAGGCCGGCGCACGTAGGCGTCGGTGCCGCCGACCAGCTTGATCACTTCGATCTCGATACGACGGTTTTCAATGTGTAGACGAATTGTGTTACCGATTACTAACAGCAGGGCAGA  >  NC_002947/5829087‑5829378

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑