Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,988,961 T→C 26.5% intergenic (+199/‑347) kefB‑III → / → PP_mr64 glutathione‑regulated potassium/H+ antiporter/C4 ncRNA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,988,9610TC26.5% 58.3 / 13.8 30intergenic (+199/‑347)kefB‑III/PP_mr64glutathione‑regulated potassium/H+ antiporter/C4 ncRNA
Reads supporting (aligned to +/- strand):  ref base T (10/12);  new base C (6/2);  total (16/14)
Fisher's exact test for biased strand distribution p-value = 2.26e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.12e-01

CGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGGGCCAGTTATTCCTGTGGGACGCGCACGCGAGCCCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGCCTGGCACAGGAGGGATTGGCCAGAAACAAACGTAGGAGCGGGTTTACCCGCGAAGCGCCGCGCGGGCGGCGCTCGATCTCATGGGCGCCCAAAAACTATCGCCAGGCACCTGGAAGCCATCCTGCAATTTCCTGACGATCACCTCGCCGCCATGACGCGAACTCA  >  NC_002947/5988821‑5989110
                                                                                                                                            |                                                                                                                                                     
cggcgCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGGGCCAATTATTCCTGCGGGATTTGCGCGCGACCGCCTTGGGGCATGTCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGACTGGTGCAGGAGGGACTGGccgaaa                                                                                                                                              <  1:78289/150‑5 (MQ=9)
     cTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGGGCCAGTTATTCCTGTGGGACGCGCACGCGAGCCCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGCCTGGCACAGGAGGGATTGGCCAGaaacaaa                                                                                                                                         <  1:317745/150‑1 (MQ=255)
      ttCGCGGGTAAACCCGCTCCTACGTTTGTTTCGGGCCAGTTATTCCTGTGGGACGCGCACGCGAGCCCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGCCTGGCACAGGAGGGATTGGCCAGaaacaaac                                                                                                                                        <  1:310852/150‑1 (MQ=255)
      ttCGCGGGTAAACCCGCTCCTAAGTTTGTTTCGGGCCAGTTATTCCTGTGGGACGCGCACGCGAGCCCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGCCTGGCACAGGAGGGATTGGCCAGaaacaaac                                                                                                                                        <  1:330493/150‑1 (MQ=255)
             gTAAACCCGCTCCTACGTTTGTTTCGGGCCAATTATTCCTGCGGGATTTGCGCGCGACCGCCTTGGGGCATGTCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGACTGGTGCAGGAGGGACTGGCC‑GAAACAAACGTAGGAGc                                                                                                                                >  1:185792/1‑150 (MQ=2)
                aaCCCGCTCCTACGTTTGTTTCGGGCCAGTTATTCCTGTGGGACGCGCACGCGAGCCCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGCCTGGCACAGGAGGGATTGGCCAGAAACAAACGTAGGAGCgg                                                                                                                              <  2:138732/150‑1 (MQ=255)
                aaCCCGCTCCTACGTTCCTTTCGGGCCAGTTATTCCTGTGGGACGCGCACGCGAGCCCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGCCTGGCACAGGAGGGATTGGCCAGAAACAAACGTAGGAGCgg                                                                                                                              <  2:245076/150‑1 (MQ=255)
                 aCCCGCTCCTACGTTTGTTTCGGGCCAGTTATTCCTGTGGGACGCGCACGCGAGCCCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGCCTGGCACAGGAGGGATTGGCCAGAAACAAACGTAGGAGCggg                                                                                                                             <  1:66471/150‑1 (MQ=255)
                  cccGCTCCTACGTTTGTTTCGGGCCAGTTATTCCTGTGGGACGCGCACGCGAGCCCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGCCTGGCACAGGAGGGATTGGCCAGAAACAAACGTAGGAGCGGGt                                                                                                                            >  2:215578/1‑150 (MQ=255)
                   ccGCTCCTACGTTTGTTTCGGGCCAATTATTCCTGCGGGATTTGCGCGCGACCGCCTTGGGGCATGTCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGACTGGTGCAGGAGGGACTGGCC‑GAAACAAACGTAGGAGCGGGttt                                                                                                                          >  2:322434/1‑150 (MQ=2)
                             gtttgtttCGGGCCAGTTATTCCTGTGGGACGCGCACGCGAGCCCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGCCTGGCACAGGAGGGATTGGCCAGAAACAAACGTAGGAGCGGGTTTACCCGCGaa                                                                                                                 >  2:407119/1‑150 (MQ=255)
                             gtttgtttCGGGCCAGTTATTCCTGTGGGACGCGCACGCGAGCCCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGCCTGGCACAGGAGGGATTGGCCAGAAACAAACGTAGGAGCGGGTTTACCCGCGaa                                                                                                                 >  1:64325/1‑150 (MQ=255)
                             gtttgtttCGGGCCAATTATTCCTGCGGGATTTGCGCGCGACCGCCTTGGGGCATGTCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGACTGGTGCAGGAGGGACTGGCC‑GAAACAAACGTAGGAGCGGGTTTACCCGCGCAg                                                                                                                >  2:447006/1‑150 (MQ=2)
                               ttgtttCGGGCCAGTTATTCCTGTGGGACGCGCACGCGAGCCCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGCCTGGCACAGGAGGGATTGGCCAGAAACAAACGTAGGAGCGGGTTTACCCGCGAAgc                                                                                                               <  1:184561/150‑1 (MQ=255)
                                  tttCGGGCCAGTTATTCCTGTGGGACGCGCACGCGAGCCCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGCCTGGCACAGGAGGGATTGGCCAGAAACAAACGTAGGAGCGGGTTTACCCGCGAAGcgcc                                                                                                            >  2:292351/1‑150 (MQ=255)
                                  tttCGGGCCAATTATTCCTGCGGGATTTGCGCGTGACCGCCTTGGGGCATGTCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGACTGGTGCAGGAGGGACTGGCC‑GAAACAAACGTAGGAGCGGGTTTACCCGCGAAGcgccg                                                                                                           >  1:64275/1‑150 (MQ=9)
                                          caattATTCCTGCGGGATTTGCGCGCGACCGCCTTGGGGCATGTCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGACTGGTAAAAGAGGGACTGGCC‑GAAACAAACGTAGGAGCGGGTTTACCCGCGAAGCGCCGCgcgggcg                                                                                                   >  2:68592/4‑150 (MQ=2)
                                             ttATTCCTGTGGGACGCGCACGCGAGCCCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGCCTGGCACAGGAGGGATTGGCCAGAAACAAACGTAGGAGCGGGTTTACCCGCGAAGCGCCGCgcgg                                                                                                      <  1:315412/145‑1 (MQ=255)
                                             ttATTCCTGTGGGACGCGCACGCGAGCCCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGCCTGGCACAGGAGGGATTGGCCAGAAACAAACGTAGGAGCGGGTTTACCCGCGAAGCGCCGCgcgg                                                                                                      >  2:315412/1‑145 (MQ=255)
                                                           cgcgCACGCGAGCCCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGCCTGGCACAGGAGGGATTGGCCAGAAACAAACGTAGGAGCGGGTTTACCCGCGAAGCGCCGCGCGGGCGGCGCTCGATCTCATgg                                                                                   <  1:407119/150‑1 (MQ=255)
                                                                         cccTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGCCTGGCACAGGAGGGATTGGCCAGAAACAAACGTAGGAGCGGGTTTACCCGCGAAGCGCCGCGCGGGCGGCGCTCGATCTCATGGGCGCCCAAAAACTa                                                                     >  1:177265/1‑150 (MQ=255)
                                                                                      cTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGCCTGGCACAGGAGGGATTGGCCAGAAACAAACGTAGGAGCGGGTTTACCCGCGAAGCGCCGCGCGGGCGGCGCTCGATCTCATGGGCGCCCAAAAACTATCGCCAGGCACCt                                                        >  1:291807/1‑150 (MQ=255)
                                                                                       tCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGACTGGTGCAGGAGGGACTGGCC‑GAAACAAACGTAGGAGCGGGTTTACCCGCGaa                                                                                                                 <  1:424638/91‑1 (MQ=18)
                                                                                       tCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGACTGGTGCAGGAGGGACTGGCC‑GAAACAAACGTAGGAGCGGGTTTACCCGCGaa                                                                                                                 >  2:424638/1‑91 (MQ=18)
                                                                                          atatCGCGTCGTACAAACAAGGCGGTCGCGCGCGCCTGGCACAGGAGGGATTGGCCAGAAACAAACGTAGGAGCGGGTTTACCCGCGAAGCGCCGCGCGGGCGGCGCTCGATCTCATGGGCGCCCAAAAACTATCGCCAGGCACCTGGaa                                                    <  1:215578/150‑1 (MQ=255)
                                                                                                          acaaGGCGGTCGCGCGCGCCTGGCACAGGAGGGATTGGCCAGAAACAAACGTAGGAGCGGGTTTACCCGCGAAGCGCCGCGCGGGCGGCGCTCGATCTCATGGGCGCCCAAAAACTATCGCCAGGCACCTGGAAGCCATCCTGCAATTTc                                    >  1:165980/1‑150 (MQ=255)
                                                                                                                    ttccagcgcCTGGCACAGGAGGGATTGGCCAGAAACAAACGTAGGAGCGGGTTTACCCGCGAAGCGCCGCGCGGGCGGCGCTCGATCTCATGGGCGCCCAAAAACTATCGCCAGGCACCTGGAAGCCATCCTGCAATTTCCTGACGATCa                          >  2:319833/6‑150 (MQ=255)
                                                                                                                     gcgcgcgcCTGGCACAGGAGGGATTGGGCAGAAACAAACGTAGGAGCGGGTTTACCCGCGAAGCGCCGCGCGGGCGGCGCTCGATCTCATGGGCGCCCAAAAACTATCGCCAGGCACCTGGAAGCCATCCTGCAATTTCCTGACGATCAc                         <  2:165980/150‑1 (MQ=255)
                                                                                                                                      gaggGATTGGCCAGAAACAAACGTAGGAGCGGGTTTACCCGCGAAGCGCCGCGCGGGCGGCGCTCGATCTCATGGGCGCCCAAAAACTATCGCCAg                                                              >  1:31296/1‑96 (MQ=255)
                                                                                                                                      gaggGATTGGCCAGAAACAAACGTAGGAGCGGGTTTACCCGCGAAGCGCCGCGCGGGCGGCGCTCGATCTCATGGGCGCCCAAAAACTATCGCCAg                                                              <  2:31296/96‑1 (MQ=255)
                                                                                                                                            ttGGCCAGAAACAAACGTAGGAGCGGGTTTACCCGCGAAGCGCCGCGCGGGCGGCGCTCGATCTCATGGGCGCCCAAAAACTATCGCCAGGCACCTGGAAGCCATCCTGCAATTTCCTGACGATCACCTCGCCGCCATGACGCGAACTca  <  1:319833/150‑1 (MQ=255)
                                                                                                                                            |                                                                                                                                                     
CGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGGGCCAGTTATTCCTGTGGGACGCGCACGCGAGCCCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGCCTGGCACAGGAGGGATTGGCCAGAAACAAACGTAGGAGCGGGTTTACCCGCGAAGCGCCGCGCGGGCGGCGCTCGATCTCATGGGCGCCCAAAAACTATCGCCAGGCACCTGGAAGCCATCCTGCAATTTCCTGACGATCACCTCGCCGCCATGACGCGAACTCA  >  NC_002947/5988821‑5989110

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑