| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 6,031,888 | A→T | 19.4% | K229M (AAG→ATG) ‡ | dppA‑IV → | dipeptide transport protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 6,031,888 | 0 | A | T | 19.4% | 66.1 / 12.6 | 31 | K229M (AAG→ATG) ‡ | dppA‑IV | dipeptide transport protein |
| Reads supporting (aligned to +/- strand): ref base A (13/12); new base T (3/3); total (16/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.79e-01 | |||||||||||
CGCAGAATACGCTGACAAGCTGCTCAAGGCCGGCACACCGGAAAAGCTCAACAACCAACCGATCGGCACCGGGCCGTTCGTGTTCCAGCGTTTCCAGAAAGACGCCGTGGTGCGCTACCGCGCCAACCCGGACTACTTCGCCGGCAAGCCGGCGGTCGACCCGCTGATCTTCGCCATCACCACCGATGCCAACGTGCGCCTGCAGAAGCTCAAGCGTGGCGAGTGCCAGGTTGCCCTGTCGCCCAAGCCGTTGGACATTGCCGAAGCGGGCCAGGACGGTAACCTCAAGG > NC_002947/6031742‑6032031 | cGCAGAATACGCTGACAAGCTGCTCAAGGCCGGCACACCGGAAAAGCTCAACAACCAACCGATCGGCACCGGGCCGTTCGTGTTCCAGCGTTTCCAGAAAGACGCCGTGGTGCGCTACCGCGCCAACCCGGACTACTTCGCCGGCAAGcc > 1:359820/1‑150 (MQ=255) tACGCTGACAAGCTGCTCAAGGCCGGCACACCGGAAAAGCTCAACAACCAACCGATCGGCACCGGGCCGTTCGTGTTCCAGCGTTTCCAGAAAGACGCCGTGGTGCGCTACCGCGCCAACCCGGACTACTTCGCCGGCTTGCcggcgaag < 2:179357/150‑4 (MQ=255) gCTGACAAGCTGCTCAAGGCCGGCACACCGGAAAAGCTCAACAACCAACCGATCGGCACCGGGCCGTTCGTGTTCCAGCGTTTCCAGAAAGACGCCGTGGTGCGCTACCGCGCCAACCCGGACTACTTCGCCGGCAAGCCGGCGGTCGAc < 1:79309/150‑1 (MQ=255) tgctCAAGGCCGGCACACCGGAAAAGCTCAACAACCAACCGATCGGCACCGGGCCGTTCGTGTTCCAGCGTTTCCAGAAAGACGCCGTGGTGCGCTACCGCGCCAACCCGGACTACTTCGCCGGCAAGCCGGCGGTCGACCCGCTGATCt > 2:65001/1‑150 (MQ=255) ctCAAGGCCGGCACACCGGAAAAGCTCAACAACCAACCGATCGGCACCGGGCCGTTCGTGTTCCAGCGTTTCCAGAAAGACGCCGTGGTGCGCTACCGCGCCAACCCGGACTACTTCGCCGGCAAGCCGGCGGTCGACCCGCTGATCTTc < 2:392407/150‑1 (MQ=255) tCAAGGCCGGCACACCGGAAAAGCTCAACAACCAACCGATCGGCACCGGGCCGTTCGTGTTCCAGCGTTTCAAGAAAGACGCCGTGGTGCGCTACCGCGCCAACCCGGACTACTTCGCCGGCAAGCCGGCGGTCGAGCCGCTGATCTTCg > 2:484257/1‑150 (MQ=255) acacCGGAAAAGCTCAACAACCAACCGATCGGCACCGGGCCGTTCGTGTTCCAGCGTTTCCAGAAAGACGCCGTGGTGCGCTACCGCGCCAACCCGGACTACTTCGCCGGCTTGCcggcgaa > 2:368358/1‑120 (MQ=255) acacCGGAAAAGCTCAACAACCAACCGATCGGCACCGGGCCGTTCGTGTTCCAGCGTTTCCAGAAAGACGCCGTGGTGCGCTACCGCGCCAACCCGGACTACTTCGCCGGCTTGCcggcgaa < 1:368358/122‑3 (MQ=255) gAAAAGCTCAACAACCAACCGATCGGCACCGGGCCGTTCGTGTTCCAGCGTTTCCAGAAAGACGCCGTGGTGCGCTAACGCGCCAACCCGGACTACATCGCCGGCAAGCCGGCGGTCGAc < 2:293675/120‑1 (MQ=255) gAAAAGCTCAACAACCAACCGATCGGCACCGGGCCGTTCGTGTTCCAGCGTTTCCAGAAAGACGCCGTGGTGCGCTAACGCGCCAACCCGGACTACATCGCCGGCAAGCCGGCGGTCGAc > 1:293675/1‑120 (MQ=255) gATCGGCACCGGGCCGTTCGTGTTCCAGCGTTTCCAGAAAGACGCCGTGGTGCGCTACCGCGCCAACCCGGACTACTTCGCCGGCTTGCcggcgaag > 1:453648/1‑94 (MQ=39) gATCGGCACCGGGCCGTTCGTGTTCCAGCGTTTCCAGAAAGACGCCGTGGTGCGCTACCGCGCCAACCCGGACTACTTCGCCGGCTTGCcggcgaag < 2:453648/97‑4 (MQ=39) aTCGGCACCGGGCCGTTCGTGTTCCAGCGTTTCCAGAAAGACGCCGTGGTGCGCTACCGCGCCAACCCGGACTACTTCGCCGGCAAGCCGGCGGTCGACCCGCTGATCTTCGCCATCACCACCGATGCCAACGTGCGCCTGCAGAAGCTc > 1:188363/1‑150 (MQ=255) gtgtTCCAGCGTTTCCAGAAAGACGCCGTGGTGCGCTACCGCGCCAACCCGGACTACTTCGCCGGCAAGCCGGCGGTCGACCCGCTGATCTTCGCCATCACCACCGATGCTAACGTGCGCCTGCAGAAGCTCAAGCGTGGCGAGAGCCAg > 1:71177/1‑150 (MQ=255) gtgtTCCAGCGTTTCCAGAAAGACGCCGTGGTGCGCTACCGCGCCAACCCGGACTACTTCGCCGGCAAGCCGGCGGTCGACCCGCTGATCTTCGCCATCACCACCGATGCCAACGTGCGCCTGCAGAAGCTCAAGCGTGGCGAGTGCCAg < 1:65001/150‑1 (MQ=255) cAGCGTTTCCAGAAAGACGCCGTGGTGCGCTACCGCGCCAACCCGGACTACTTCGCCGGCAAGCCGGCGGTCGACCCGCTGATCTTCGCCATCACCACCGATGCCAACGTGCGCCTGCAGAAGCTCAAGCGTGGCGAGTGCCAGGTTGcc < 2:295987/150‑1 (MQ=255) gCGTTTCCAGAAAGACGCCGTGGTGCGCTACCGCGCCAACCCGGACTACTTCGCCGGCAAGCCGGCGGTCGACCCGCTGATCTTCGCCATCACCACCGATGCCAACGTGCGCCTGCAGAAGCTCAAGCGTGGCGAGTGCCAGGTTGCCCt < 2:359820/150‑1 (MQ=255) aGAAAGACGCCGTGGTGCGCTACCGCGCCAACCCGGACTACTTCGCCGGCAAGCCGGCGGTCGACCCGCTGATCTTCGCCATCACCACCGATGc > 2:154240/1‑94 (MQ=255) aGAAAGACGCCGTGGTGCGCTACCGCGCCAACCCGGACTACTTCGCCGGCAAGCCGGCGGTCGACCCGCTGATCTTCGCCATCACCACCGATGc < 1:154240/94‑1 (MQ=255) gCCGTGGTGCGCTACTACGCCAACCCGGACTACTTCGCCGGCAAGCCGGCGGTCGACCCGCTGATCTTCGCCATCACCACCGATGCCAACGTGCGCCTGCAGAAGCTCAAGCGTGGCGAGTGCCAGGTTGCCCTGTCGCCCAAGCCGTTg > 2:45309/1‑150 (MQ=255) gCCGTGGTGCGCTACCGCGCCAACCCGGACTACTTCGCCGGCAAGCCGGCGGTCGACCCGCTGATCTTc < 1:83364/69‑1 (MQ=255) gCCGTGGTGCGCTACCGCGCCAACCCGGACTACTTCGCCGGCAAGCCGGCGGTCGACCCGCTGATCTTc > 2:83364/1‑69 (MQ=255) gcgcTACCGCGCCAACCCGGACTACTTCGCCGGCAAGCCGGCGGTCGACCCGCTGATCTTCGCCATCACCACCGATGCCAACGTGCGCCTGCAGAAGCTCAAGCGTGGCGAGTGCCAGGTTGCCCTGTCGCCCAAGCCGTTGGACATTGc < 2:66144/150‑1 (MQ=255) gcTACCGCGCCAACCCGGACTACTTCGCCGGCAAGCCGGCGGTCGACCCGCTGATCTTCGCCATCACCACCGATGCCAACGTGCGCCTGCAGAAGCTCAAGCGTGGCGAGTGCCAGGTTGCCCTGTCGCCCAAGCCGTTg > 2:289558/1‑140 (MQ=255) gcTACCGCGCCAACCCGGACTACTTCGCCGGCAAGCCGGCGGTCGACCCGCTGATCTTCGCCATCACCACCGATGCCAACGTGCGCCTGCAGAAGCTCAAGCGTGGCGAGTGCCAGGTTGCCCTGTCGCCCAAGCCGTTg < 1:289558/140‑1 (MQ=255) tACCGCGCCAACCCGGACTACTTCGCCGGCAAGCCGGCGGTCGACCCGCTGATCTTCGCCATCACCACCGATGCCAACGTGCGCCTGCAGAAGCTCAAGCGTGGCGAGTGCCAGGTTGCCCTGTCGCCCAAGCCGTTGGACATTGCCGaa > 2:236099/1‑150 (MQ=255) ccGGACTACTTCGCCGGCAAGCCGGCGGTCGACCCGCTGATCTTCGCCATCACCACCGATGCCAACGTGCGCCTGCAGAAGCTCAAGCGTGGCGAGTGCCAGGTTGCCCTGTCGCCCAAGCCGTTGGACATTGCCGAAGCGGGCCAGGAc > 1:249034/1‑150 (MQ=255) cGGACTACTTCGCCGGCAAGCCGGCGGTCGACCCGCTGATCTTCGCCATCACCACCGATGCCAACGTGCGCCTGCAGAAGCTCAAGCGTGGCGAGTGCCAGGTTGCCCTGTCGCCCAAGCCGTTGGACATTGCCGAAGCGGGCCAGGACg < 1:236099/150‑1 (MQ=255) ctTCGCCGGCAAGCCGGCGGTCGACCCGCTGATCTTCGCCATCACCACCGATGCCAACGTGCGCCTGCAGAAGCTCAAGCGTGGCGAGTGCCAGGTTGCCCTGTCGCCCAAGCCGTTGGACATTGCCGAAGCGGGCCAGGACGGTAACCt > 1:54165/1‑150 (MQ=255) cGCCGGCAAGCCGGCGGTCGACCCGCTGATCTTCGCCATCACCACCGATGCCAACGTGCGCCTGCAGAAGCTCAAGCGTGGCGAGTGCCAGGTTGCCCTGTCGCCCAAGCCGTTGGACATTGCCGAAGCGGGCCAGGACGGTAACCTCaa < 2:54165/150‑1 (MQ=255) ccGGCTTGCCGGCGGTCGACCCGCTGATCTTCGCCATCACCACCGATGCCAACGTGCGCCTGCAGAAGCTCAAGCGTGGCGAGTGCCAGGTTGCCCTGTCGCCCAAGCCGTTGGACATTGCCGAAGCGGGCCAGGACGGTAACCTCAAgg > 2:108477/1‑150 (MQ=255) | CGCAGAATACGCTGACAAGCTGCTCAAGGCCGGCACACCGGAAAAGCTCAACAACCAACCGATCGGCACCGGGCCGTTCGTGTTCCAGCGTTTCCAGAAAGACGCCGTGGTGCGCTACCGCGCCAACCCGGACTACTTCGCCGGCAAGCCGGCGGTCGACCCGCTGATCTTCGCCATCACCACCGATGCCAACGTGCGCCTGCAGAAGCTCAAGCGTGGCGAGTGCCAGGTTGCCCTGTCGCCCAAGCCGTTGGACATTGCCGAAGCGGGCCAGGACGGTAACCTCAAGG > NC_002947/6031742‑6032031 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |