| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 6,104,109 | T→A | 11.4% | intergenic (+31/‑83) | PP_5353 → / → PP_5354 | hypothetical protein/hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 6,104,109 | 0 | T | A | 11.4% | 94.3 / 4.2 | 35 | intergenic (+31/‑83) | PP_5353/PP_5354 | hypothetical protein/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base T (14/17); new base A (2/2); total (16/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.25e-01 | |||||||||||
GAAGAATTCGCGCTTCGACCAGGGCGAAGTGTTCAGCGAAAGCTGGCACATGGTTCGCGCCCAAGGCGAGAACCAGCCTTGGCTGGTAGCCGGTATCCGTCAAAACAGCTAATCGCTGCGTGCTAAACACAGAACCCCGGGCTTGCCCGGGGTTTTTGCTTTTGCCAAAGTGGGCTACTAGGGTATAAGGCGCAGCGTTGTCATCTAGGTCAGAGGAAGTGAACCGTGGAAGAAGTGATCGAACAACTCCGTGAAGCGAATGAGCCGGTACCGGTGCCCCTGGAGCTTCCC > NC_002947/6103967‑6104257 | gaagaaTTCGCGCTTCGACCAGGGCGAAGTGTTCAGCGAAAGCTGGCACATGGTTCCCGGTCAAGGCGAGAACCAGCCTTGGCTGGTAGCCGGTATCCGTCAAAACAGCTAATCGCTGCGTGCTAAACACAGAACCCCGGGCTTGCCCgg < 1:134360/150‑1 (MQ=255) ttCGCGCTTCGACCAGGGCGAAGTGTTCAGCGAAAGCTGGCACATGGTTCGCGCCCAAGGCGAGAACCAGCCTTGGCTGAATATCTGTATCCGTCAAAACAGCTAATCGCTGCGTGCTAAACACAGAACCCCGGGCTTGCCCGGGGtttt > 1:286409/1‑150 (MQ=255) aCCAGGGCGAAGTGTTCAGCGAAAGCTGGCACATGGTTCGCGCCCAAGGCGAGAACCAGCCTTGGCTGGTAGCCGGTATCCGTCAAAACAGCTAATCGCTGCGTGCTAAACACAGAACCCCGGGCAAGCCCGGGGttctgt > 2:437912/1‑137 (MQ=255) aCCAGGGCGAAGTGTTCAGCGAAAGCTGGCACATGGTTCGCGCCCAAGGCGAGAACCAGCCTTGGCTGGTAGCCGGTATCCGTCAAAACAGCTAATCGCTGCGTGCTAAACACAGAACCCCGGGCAAGCCCGGGGttctgt < 1:437912/141‑5 (MQ=255) ggCGAAGTGTTCAGCGAAAGCTGGCACATGGTTCGCGCCCAAGGCGAGAACCAGCCTTGGCTGGTAGCCGGTATCCGTCAAAACAGCTAATCGCTGCGTGCTAAACACAGAACCCCGGGCTTGCCCGGGGTTTTTGCTTTTGCCAAAGTg > 2:88302/1‑150 (MQ=255) tgcAGCGAAAGCTGGCACATGGTTCGCGCCCAAGGCGAGAACCAGCCTTGGCTGGTAGCCGGTATCCGTCAAAACAGCTAATCGCTGCGTGCTAAACACAGAACCCCGGGCTTGCCCGGGGTTTTTGCTTTTGCCAAAGTGGGCTACTAg > 1:225954/3‑150 (MQ=255) ggCACATGGTTCGCGCCCAAGGCGAGAACCAGCCTTGGCTGGTAGCCGGTATCCGTCAAAACAGCTAATCGCTGCGTGCTAAACACAGAACCCCGGGCTTGCCCGGGGTTTTTGCTTTTGCCAAAGTGGGCTACTAGGGTATAAGGCGCa < 1:199176/150‑1 (MQ=255) aTGGTTCGCGCCCAAGGCGAGAACCAGCCTTGGCTGGTAGCCGGTATCCGTCAAAACAGCTAATCGCTGCGTGCTAAACACAGAACCCCGGGCTTGCCCGGGGTTTTTGCTTTTGCCAAAGTGGGCTACTAGGGTATAAGGCGCAGCGtt < 2:144636/150‑1 (MQ=255) gcCCAAGGCGAGAACCAGCCTTGGCTGGTAGCCGGTATCCGTCAAAACAGCTAATCGCTGCGTGCTAAACACAGAACCCCGGGCTTGCCCGGGGTTTTTGCTTTTGCCAAAGTGGGCTACTAGGGTATAAGGCGCAGCGTTGTCATCTAg < 2:188855/150‑1 (MQ=255) agaACCAGCCTTGGCTGGTAGCCGGTATCCGTCAAAACAGCTAATCGCTGCGTGCTAAACACAGAACCCCGGGCTTGCCCGGGGTTTTTGCTTTTGCCAAAGTGGGCTACTAGGGTATAAGGCGCAGCGTTGTCATCTAGGTCAGAGGaa > 1:75763/1‑150 (MQ=255) ccAGCCTTGGCTGGTAGCCGGTATCCGTCAAAACAGCTAATCGCTGCGTGCTAAACACAGAACCCCGGGCTTGCCCGGGGttttt > 2:61247/1‑85 (MQ=255) ccAGCCTTGGCTGGTAGCCGGTATCCGTCAAAACAGCTAATCGCTGCGTGCTAAACACAGAACCCCGGGCTTGCCCGGGGttttt < 1:61247/85‑1 (MQ=255) cTGGTAGCCGGTATCCGTCAAAACAGCTAATCGCTGCGTGCTAAACACAGAACCCCGGGCTTGCCCGGGGTTTTTGCTTTTGCCAAAGTGGGCTACTAGGGTATAAGGCGCAGCGTTGTCATCTAGGTCAGAGGAAGTGAACCGTGgaag < 2:357371/150‑1 (MQ=255) tAGCCGGTATCCGTCAAAACAGCTAATCGCTGCGTTCTAAACACAGAACCCCGGGCTTGCCCGGGGGTTCTGCTGTTGCCAAAGTGGGCTACTAGGGTATAAGGCGCAGCGTTGTCATCTAGGTCAGAGGAAGTGAACCGTGGAAGAAGt < 1:88302/150‑1 (MQ=255) ccGGTATCCGTCAAATTAGCTAATCGCTGCGTGCTAAACACAGAACCCCGGGCTTGCCCGGTGTTTTTGCTTTTGCCAAAGTGGGCTACTAGGGTATAAGGCGCAGCGTTGTCATCTAGGTCAGAGGAAGTGAACCGTGGAAGAAGTGAt < 2:131195/150‑1 (MQ=255) ccGGTATCCGTCAAAACAGCTAATCGCTGCGTGCTAAACACAGAACCCCGGGCTTGCCCGGGGTTTTTGCTTTTGCCAAAGTGGGCTACTAGGGTATAAGGCGCAGCGTTGTCATCTAGGTCAGAGGAAGTGAACCGTGGAAGAAGTGAt < 2:225954/150‑1 (MQ=255) gTATCCGTCAAAACAGCTAATCGCTGCGTGCTAAACACAGAACCCCGGGCTTGCCCGGGGTTTTTGCTTTTGCCAAAGTTGGCTACTAGGGTATAAGGCGCAGCGTTGTCATCTAGGTCAGAGGAAGTGAACAGTGGAAGAAGTGATCGa > 2:418388/1‑150 (MQ=255) tCCGTCAAAACAGCTAATCGCTGCGTGCTAAACACAGAACCCCGGGCTTGCCCGGGGTTTTTGCTTTTGCCAAAGTGGGCTACTAGGGTATAAGGCGCAGCGTTGTCATCTAGGTCAGAGGAAGTGAACCGTGGAAGAAGTGAt > 2:459082/1‑144 (MQ=255) tCCGTCAAAACAGCTAATCGCTGCGTGCTAAACACAGAACCCCGGGCTTGCCCGGGGTTTTTGCTTTTGCCAAAGTGGGCTACTAGGGTATAAGGCGCAGCGTTGTCATCTAGGTCAGAGGAAGTGAACCGTGGAAGAAGTGAt < 1:459082/144‑1 (MQ=255) cGTCAAAACAGCTAATCGCTGCGTGCTAAACACAGAACCCCGGGCTTGCCCGGGGTTTTTGCTTTTGCCAAAGTGGGCTACTAGGGTATAAGGCGCAGCGTTGTCATCTAGGTCAGAGGAAg > 2:160813/1‑122 (MQ=255) cGTCAAAACAGCTAATCGCTGCGTGCTAAACACAGAACCCCAGGCTTGCCCGGGGTTTTTGCTTTTGCCAAAGTGGGCTACTAGGGTATAAGGCGCAGCGTTGTCATCTAGGTCAGAGGAAg < 1:160813/122‑1 (MQ=255) gTCAAAACAGCTAATCGCTGCGTGCTAAACACAGAACCCCGGGCTTGCCCGGGGTTTTTGCTTTTGCCAAAGTGGGCTACTAGGGTATAAGGCGCAGCGTTGTCATCTAGGTCAGAGGAAGTGAACCGTGGAAGAAGTGATCGAACAACt > 1:168249/1‑150 (MQ=255) tCAAAACAGCTAATCGCTGCGTGCTAAACACAGAACCCCGGGCTTGCCCGGGGTTTTTGCTTTTGCCAAAGTGGGCTACTAGGGTATAAGGCGCAGCGTTGTCATCTAGGTCAGAGGAAGTGAACCGTGGAAGAAGTGATCGAACAACTc > 1:107809/1‑150 (MQ=255) aaCAGCTAATCGCTGCGTGCTAAACACAGAACCCCGGGCTTGCCCGGGGTTTTTGCTTTTGCCAAAGTGGGCTACTAGGGTATAAGGCGCAGCGTTGTCATCTAGGTCAGAGGAAGTGAACCGTGGAAGAAGTGATCGAACAACTCCGTg < 2:252168/150‑1 (MQ=255) aaTCGCTGCGTGCTAAACACAGAACCCCGGGCTTGCCCGGGGTTTTTGCTTTTGCCAAAGTGGGCTACTAGGGTATAAGGCGCAGCGTTGTCATCTAGGTCAGAGGAAGTGAACCGTGGAAGAAGTGATCGAACAACTCCGTGAAGCGaa < 2:107809/150‑1 (MQ=255) tGCGCGCTAAACACAGAACCCCCGGCTTGCCCGGGGTTTTTGCTTTTGCCAAAGTGGGCTACTAGGGTATAAGGCGCAGCGTTGCCATCTAGGTCAGAGGAAGTGAACCGGGGAAGAAGTGATCGAACAACTCCGTGAAGCGAATGAGcc < 1:449686/150‑1 (MQ=255) aaaCACAGAACCCCGGGCTTGCCCGGGGTTTTTGCTTTTGCCAAAGTGGGCTACTAGGGTATAAGGCGCAGCGTTGTCATCTAGGTCAGAGGAAGTGAACCGTGGAAGAAGTGATCGAACAACTCCGTGAAGCGAATGAGCCGGTACCgg > 2:489254/1‑150 (MQ=255) acacAGAACCCCGGGCTTGCCCGGGGTTTTTGCTTTTGCCAAAGTGGGCTACTAGGGTATAAGGCGCAGCGTTGTCATCTAGGt < 2:35524/84‑1 (MQ=255) acacAGAACCCCGGGCTTGCCCGGGGTTTTTGCTTTTGCCAAAGTGGGCTACTAGGGTATAAGGCGCAGCGTTGTCATCTAGGt > 1:35524/1‑84 (MQ=255) acacAGAACCCCGGGCTTGCCCGGGGTTTTTGCTTTTGCCAAAGTGGGCTACTAGGGTATAAGGCGCAGCGTTGTCATCTAGGTCAGAGGAAGTGAACCGTGGAAGAAGTGATCGAACAACTCCGTGAAGCGAATGAGCCGGTACCGGTg < 2:168249/150‑1 (MQ=255) aaCCCCGGGCAAGCCCGGGGTTTTTGCTTTTGCCAAAGTGGGCTACTAGGGTATAAGGCGCAGCGtt > 2:283415/1‑67 (MQ=38) aaCCCCGGGCAAGCCCGGGGTTTTTGCTTTTGCCAAAGTGGGCTACTAGGGTATAAGGCGCAGCGtt < 1:283415/67‑1 (MQ=38) cccGGGCTTGCCCGGGGTTTTTGCTTTTGCCAAAGTGGGCTACTAGGGTATAAGGCGCAGCGTTGTCATCTAGGTCAGAGGAAGTGAACCGTGGAAGAAGTGATCGAACAACTCCGTGAAGCGAATGAGCCGGTACCGGTGCCCCTGGAg > 2:327614/1‑150 (MQ=255) cccGGGCTTGCCCGGGGTTTTTGCTTTTGCCAAAGTGGGCTACTAGGGTATAAGGCGCAGCGTTGTCATCTAGGTCAGAGGAAGTGAACCGTGGAAGAAGTGATCGAACAACTCCGTGAAGCGAATGAGCCGGTACCGGTGCCCCTGGAg > 1:332013/1‑150 (MQ=255) cTTGCCCGGGGTTTTTGCTTTTGCCAAAGTGGGCTACTAGGGTATAAGGCGCAGCGTTGTCATCTAGGTCAGAGGAAGTGAACCGTGGAAGAAGTGATCGAACAACTCCGTGAAGCGAATGAGCCGGTACCGGTGCCCCTGGAGCTTccc < 2:75763/150‑1 (MQ=255) | GAAGAATTCGCGCTTCGACCAGGGCGAAGTGTTCAGCGAAAGCTGGCACATGGTTCGCGCCCAAGGCGAGAACCAGCCTTGGCTGGTAGCCGGTATCCGTCAAAACAGCTAATCGCTGCGTGCTAAACACAGAACCCCGGGCTTGCCCGGGGTTTTTGCTTTTGCCAAAGTGGGCTACTAGGGTATAAGGCGCAGCGTTGTCATCTAGGTCAGAGGAAGTGAACCGTGGAAGAAGTGATCGAACAACTCCGTGAAGCGAATGAGCCGGTACCGGTGCCCCTGGAGCTTCCC > NC_002947/6103967‑6104257 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |