| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 865,409 | A→C | 23.5% | V140V (GTT→GTG) | upp ← | uracil phosphoribosyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 865,409 | 0 | A | C | 23.5% | 20.7 / 6.5 | 17 | V140V (GTT→GTG) | upp | uracil phosphoribosyltransferase |
| Reads supporting (aligned to +/- strand): ref base A (8/5); new base C (2/2); total (10/7) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.22e-01 | |||||||||||
GATCTTCACGTCCGGGTGGGCTTTTTCCACCACCTCGATGCCTTCTGGTGCGGCGACCAGCACCATGGCGCGAATCTCTTTGCAGCCGGCCTTTTTCAGCAGGTCGATGGTGGCAACCATCGAGCCGCCGGTGGCCAGCATCGGGTCGATGATCAGGGCCAGGCGCTGGTTGATGTCCGGCGCGAGCTTTTCCAGATAGGTGTGGGCTTCGAGGGTTTCTTCGTTTCGGGCAACGCCGACGGCGCTGACCTTGGCCCCCGGGA > NC_002947/865295‑865557 | gATCTTCACGTCCGGGTGGGCTTTTTCCACCACCTCGATGCCTTCTGGTGCGGCGACCAGCACCATGGCGCGAATCTCTTTGCAGCCGGCCTTTTTCAGCAGGTCGATGGTGGCAACCATCGAGCCGCCGGTGGCCAGCATCGGGTCgat > 2:98739/1‑150 (MQ=255) cTTTTTCCACCACCTCGATGCCTTCTGGTGCGGCGACCAGCACCATGGCGCGAATCTCTTTGCAGCCGGCCTTTTTCAGCAGGTCGATGGTGGCAACCATCGAGCCGCCGGTGGCCAGCATCGGGTCGATGATCAGGGCCAGGCGCTGGt > 2:433317/1‑150 (MQ=255) cccTCGATGCCTTCTGGTGCGGCGACCAGCACCATGGCGCGAATCTCTTTGCAGCCGGCCTTTTTCAGCAGGTCGATGGTGGCAACCATCGAGCCGCCGGTGGCCAGCATCGGGTCGATGATCAGGGCCAGGCGCTGGTTGATGTCCGgc < 2:82211/149‑1 (MQ=255) cTGGTGCGGCGACCAGCACCATGGCGCGAATCTCTTTGCAGCCGGCCTTTTTCAGCAGGTCGATGGTGGCAACCATCGAGCCGCCGGTGGCCAGCATCGGGTCGATGATCAGGGCCAGGCGCTGGTTGATGTCCGGCGCGAGCTTTTCCa < 2:349389/150‑1 (MQ=255) ggcgACCAGCACCATGGCGCGAATCTCTTTGCGGCCCATCTTTTTCAGCAGGTCGATGGTGGCAACCATCGAGCCGCCGGTGGCCAGCATCGGGTCGATGATCAGGGCCAGGCGCTGGTTGATGTCCGGCGCGAGCTTTTCCAGATAGgt < 2:40938/150‑1 (MQ=255) cAGCACCATGGCGCGAATCTCTTTGCAGCCGGCCTTTTTCAGCAGGTCGATGGTGGCAACCATCGAGCCGCCGGTGGCCAGCATCGGGTCGATGATCAGGGCCAGGCGCTGGTTGATGTCCGGCGCGAGCTTTTCCAGATAGGTGTGGGc > 2:179408/1‑150 (MQ=255) aGCACCATGGCGCGAATCTCTTTGCAGCCGGCCTTTTTCAGCAGGTCGATGGTGGCAACCATCGAGCCGCCGGTGGCCAGCATCGGGTCGATGATCAGGGCCAGGCGCTGGTTGATGTCCGGCGCGAGCTTTTCCAGATAGGTGTGGGCt > 2:172397/1‑150 (MQ=255) gAATCTCTTTGCAGCCGGCCTTTTTCAGCAGGTCGATGGTGGCAACCATCGAGCCGCCGGTGGCCAGCATCGGGTCGATGATCAGGGCCAGGCGCTGGTTGATGTCCGGCGCGAGCTTTTCCAGATAGGTGTGGGCTTCGAGGGTttctt > 1:87604/1‑150 (MQ=255) aTCTCTTTGCAGCCGGCCTTTTTCAGCAGGTCGATGGTGGCAACCATCGAGCCGCCGGTGGCCAGCATCGGGTCGATGATCAGGGCCAGGCGCTGGTTGATGTCCGGCGCGAGCTTTTCCAGATAGGTGTGGGCTTCGAGGGTTTCTTCg > 1:159219/1‑150 (MQ=255) aGCCGGCCTTTTTCAGCAGGTCGATGGTGGCAACCATCGAGCCGCCGGTGGCCAGCATCGGGTCGATGATCAGGGCCAGGCGCTGGTTGATGTCCGGCGCGAGCTTTTCCAGATAGGTGTGGGCTTCGAGGGTTTCTTCGTTTCGGGCaa > 1:502309/1‑150 (MQ=255) agGTCGATGGTGGCAACCATCGAGCCGCCGGTGGCCAGCATCGGGTCGATGATCAGGGCCAGGCGCTGGTTGATGTCCGGCGCGAGCTTTTCCAGATAGGTGTGGGCTTCGAGGGTTTCTTCGTTTCGGGCAACGCCGACGGCGCTGAcc < 2:87604/150‑1 (MQ=255) ctCGATGGTTGCCACCATCGAGCCGCCGGTGGCCAGCATCGGGTCGATGATCAGGGCCAGGCGCTGGTTGATGTCCGGCGCGAGCTTTTCCAGATAGGTGTGGGCTTCGAGGGTTTCTTCGTTTCgg > 2:115608/2‑127 (MQ=255) ctCGATGGTTGCCACCATCGAGCCGCCGGTGGCCAGCATCGGGGCGATGATCAGGGCCAGGCGCTGGTTGATGTCCGGCGCGAGCTTTTCCAGATAGGTGTGGGCTTCGAGGGTTTCTTCGTTTCgg < 1:115608/126‑1 (MQ=255) tCGATGGTTGCCACCATCGAGCCGCCGGTGGCCAGCATCGGGTCGATGATCAGGGCCAGGCGCTGGTTGATGTCCGGCGCGAGCTTTTCCAGATAGGTGTGGGCTTCGAgg < 1:71147/111‑1 (MQ=255) tCGATGGTTGCCACCATCGAGCCGCCGGTGGCCAGCATCGGGTCGATGATCAGGGCCAGACGCTGGTTGATGTCCGGCGCGAGCTTTTCCAGATAGGTGTGGGCTTCGAgg > 2:71147/1‑111 (MQ=255) tggCAACCATCGAGCCGCCGGTGGCCAGCATCGGGTCGATGATCAGGGCCAGGCGCTGGTTGATGTCCGGCGCGAGCTTTTCCAGATAGGTGTGGGCTTCGAGGGTTTCTTCGTTTCGGGCAACGCCGACGGCGCTGACCTTGGCCCCCg > 1:137727/1‑150 (MQ=255) cAACCATCGAGCCGCCGGTGGCCAGCATCGGGTCGATGATCAGGGCCAGGCGCTGGTTGATGTCCGGCGCGAGCTTTTCCAGATAGGTGTGGGCTTCGAGGGTTTCTTCGTTTCGGGCAACGCCGACGGCGCTGACCTTGGCCCCCggaa < 1:98739/150‑3 (MQ=255) aaCCATCGAGCCGCCGGTGGCCAGCATCGGGTCGATGATCAGGGCCAGGCGCTGGTTGATGTCCGGCGCGAGCTTTTCCAGATAGGTGTGGGCTTCGAGGGTTTCTTCGTTTCGGGCAACGCCGAc < 1:449025/126‑1 (MQ=255) aaCCATCGAGCCGCCGGTGGCCAGCATCGGGTCGATGATCAGGGCCAGGCGCTGGTTGATGTCCGGCGCGAGCTTTTCCAGATAGGTGTGGGCTTCGAGGGTTTCTTCGTTTCGGGCAACGCCGAc > 2:449025/1‑126 (MQ=255) | GATCTTCACGTCCGGGTGGGCTTTTTCCACCACCTCGATGCCTTCTGGTGCGGCGACCAGCACCATGGCGCGAATCTCTTTGCAGCCGGCCTTTTTCAGCAGGTCGATGGTGGCAACCATCGAGCCGCCGGTGGCCAGCATCGGGTCGATGATCAGGGCCAGGCGCTGGTTGATGTCCGGCGCGAGCTTTTCCAGATAGGTGTGGGCTTCGAGGGTTTCTTCGTTTCGGGCAACGCCGACGGCGCTGACCTTGGCCCCCGGGA > NC_002947/865295‑865557 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |