Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 937,766 A→T 13.2% L2620Q (CTG→CAG)  PP_0806 ← surface adhesion protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947937,7660AT13.2% 100.5 / 8.3 38L2620Q (CTG→CAG) PP_0806surface adhesion protein
Reads supporting (aligned to +/- strand):  ref base A (16/17);  new base T (3/2);  total (19/19)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.77e-01

CGCTGATGCTGCTGCCATCGGCAGCGACGACCAGTTCGCTCGGGCTGGTCGGCGTGACCACCAGTGGCACGTCGTAGGTCAGCGCTTCAGAGGCGTTGCCGCTCGGGTCGGTCTGTACCAGGCTCAGGCGTTCACCCGGCAGCGCAGCCGGGTCGAGGTCGATCAGGAAAGTACCGTTCGCGGTCACTGTGCCGGTACCGACCAGCGTGCCATTGGCATCGCGCACCTCGACGGTAGCACCGGCTTCGCCGCGACCGCCCAAGGTCAGGCCGTCGGCGCCGACGA  >  NC_002947/937626‑937910
                                                                                                                                            |                                                                                                                                                
cGCTGATGCTGCTGCCATCGGCAGCGACGACCAGTTCGCTCGGGCTGGTCGGCGTGACCACCAGTGGCACGTCGTAGGTCAGCGCTTCAGAGGCGTTGCCGCTCGGGTCGGTCTGTACCAGGCTCAGGCGTTCACCCGGCAGCGCAGCCg                                                                                                                                         >  2:275449/1‑150 (MQ=255)
 gCTGATGCTGCTGCCATCGGCACCGACGACCAGTTCGCTCGGGCTGGTCGGCGTGACCACCAGTGGCACGTCGTAGGTCAGCGCTTCAGAGGCGTTGCCGCTCGGGTCGGTCTGTACCAGGCTCAGGCGTTCACCCGGCAGCGCAGCCgg                                                                                                                                        <  1:440593/150‑1 (MQ=255)
    gATGCTGCTGCCATCGGCAGCGACGACCAGTTCGCTCGGGCTGGTCGGCGTGACCACCAGTGGCACGTCGTAGGTCAGCGCTTCAGAGGCGTTGCCGCTCGGGTCGGTCTGTACCAGGCTCAGGCGTTCACCCGGCAGCGCAGCCGggtc                                                                                                                                     >  1:289289/1‑150 (MQ=255)
      tgctgctgcCATCGGCAGCGACGACCAGTTCGCTCGGGCTGGTCGGCGTGACCACCAGTGTCACGTCGTAGGTCAGCGCTTCAGAGGCGTTGCCGCTCGGGTCGGTCTGTACCAGGCTCAGGCGTTCACCCGGCTGCGCTGCCGggtgaa                                                                                                                                   <  1:290036/150‑4 (MQ=255)
      tgctgctgcCATCGGCAGCGACGACCAGTTCGCTCGGGCTGGTCGGCGTGACCACCAGTGGCACGTCGTAGGTCAGCGCTTCAGAGGCGTTGCCGCTCGGGTCGGTCTGTACCAGGCTCAGGCGTTCACCCGGCAGCGCAGCCGggtcga                                                                                                                                   <  1:342307/150‑1 (MQ=255)
         tgctgcCATCGGCAGCGACGACCAGTTCGCTCGGGCTGGTCGGCGTGACCACCAGTGGCACGTCGTAGGTCAGCGCTTCAGAGGCGTTGCCGCTCGGGTCGGTCTGTACCAGGCTCAGGCGTTCACCCGGCAGCGCAGCCGggtcgaggt                                                                                                                                >  2:355207/1‑150 (MQ=255)
          gctgcCATCGGCAGCGACGACCAGTTCGCTCGGGCTGGTCGGCGTGACCACCAGTGGCACGTCGTAGGTCAGCGCTTCAGAGGCGTTGCCGCTCGGGTCGGTCTGTACCAGGCTCAGGCGTTCACCCGGCAGCGCAGCCGggtcgaggtc                                                                                                                               <  2:497578/150‑1 (MQ=255)
               cATCGGCAGCGACGACCAGTTCGCTCGGGCTGGTCGGCGTGACCACCAGTGGCACGTCGTAGGTCAGCGCTTCAGAGGCGTTGCCGCTCGGGTCGGTCTGTACCAGGCTCAGGCGTTCACCCGGCAGCGCAGCCGGGTCGAGGTCGATCa                                                                                                                          >  2:357188/1‑150 (MQ=255)
                 tCGGCAGCGACGACCAGTTCGCTCGGGCTGGTCGGCGTGACCACCAGTGGCACGTCGTAGGTCAGCGCTTCAGAGGCGTTGCCGCTCGGGTCGGTCTGTACCAGGCTCAGGCGTTCACCCGGCAGCGCAGCCGGGTCGAGGTCGATCAgg                                                                                                                        <  2:64538/150‑1 (MQ=255)
                           cgacCAGTTCGCTCGGGCTGGTCGGCGTGACCACCAGTGGCACGTCGTAGGTCAGCGCTTCAGAGGCGTTGCCGCTCGGGTCGGTCTGTACCAGGCTCAGGCGTTCACCCGGCAGCGCAGCCGGGTCGAGGTCGATCAGGAAAGTACCGt                                                                                                              >  2:42275/1‑150 (MQ=255)
                                  ttCGCTCGGGCTGGTCGGCGTGACCACCAGTGGCACGTCGTAGGTCAGCGCTTCAGAGGCGTTGCCGCTCGGGTCGGTCTGTACCAGGCTCAGGCGTTCACCCGGCAGCGCAGCCgg                                                                                                                                        <  1:490679/117‑1 (MQ=255)
                                  ttCGCTCGGGCTGGTCGGCGTGACCACCAGTGGCACGTCGTAGGTCAGCGCTTCAGAGGCGTTGCCGCTCGGGTCGGTCTGTACCAGGCTCAGGCGTTCACCCGGCAGCGCAGCCgg                                                                                                                                        >  2:490679/1‑117 (MQ=255)
                                  ttCGCTCGGGCTGGTCGGCGTGACCACCAGTGGCACGTCGTAGGTCAGCGCTTCAGAGGCGTTGCCGCTCGGGTCGGTCTGTACCAGGCTCAGGCGTTCACCCGGCAGCGCAGCCGGGTCGAGGTCGATCAGGAAAGTACCGTTCGCGGt                                                                                                       <  1:355207/150‑1 (MQ=255)
                                      cTCGGGCTGGTCGGCGTGACCACCAGTGGCACGTCGTAGGTCAGCGGTTCAGAGGCGTTGCCGCTCGGGTCGGTCTGTACCAGGCTCAGGCGTTCACCCGGCAGCGCAGCCGGGTCGAGGTCGATCAGGAAAGTACCGTTCGCGGTCACt                                                                                                   <  1:357188/150‑1 (MQ=255)
                                          ggCTGGTCGGCGTGACCACCAGTGGCACGTCGTAGGTCAGCGCTTCAGAGGCGTTGCCGCTCGGGTCGGTCTGTACCAGGCTCAGGCGTTCACCCGGCAGCGCAGCCGGGTCGAGGTCGATCAGGAAAGTACCGTTCGCGGTCACTGTGc                                                                                               <  1:156534/150‑1 (MQ=255)
                                                      tGACCACCAGTGGCACGTCGTAGGTCAGCGCTTCAGAGGCGTTGCCGCTCGGGTCGGTCTGTACCAGGCTCAGGCGTTCACCCGGCAGCGCAGCCGGGTCGAGGTCGATCAGGAAAGTACCGTTCGCGGTCACTGTGCCGGTACCGACCa                                                                                   <  2:289289/150‑1 (MQ=255)
                                                             caGTGGCACGTCGTAGGTCAGCGCTTCAGAGGCGTTGCCGCTCGGGTCGGTCTGTACCAGGCTCAGGCGTTCACCCGGCAGCGCAGCCGGGTCGAGGTCGATCAGGAAAGTACCGTTCGCGGTCACTGTGCCGGTCCCGCCCAGCGTGcc                                                                            >  2:165053/1‑150 (MQ=255)
                                                                tGGCACGTCGTAGGTCAGCGCTTCAGAGGCGTTGCCGCTCGGGTCGGTCTGTACCAGGCTCAGGCGTTCACCCGGCAGCGCAGCCGGGTCGAGGTCGATCAGGAAAGTACCGTTCGCGGTCACTGTGCCGGTACCGACCAGCGTGCCAtt                                                                         <  1:42275/150‑1 (MQ=255)
                                                                              tCAGCGCTTCAGAGGCGTTGCCGCTCGGGTCGGTCTGTACCAGGCTCAGGCGTTCACCCGGCAGCGCAGCCGGGTCGAGGTCGATCAGGAAAGTACCGTTCGCGGTCACTGTGCCGGTACCGACCAGCGTGCCATTGGCATc                                                                   >  1:348286/1‑142 (MQ=255)
                                                                              tCAGCGCTTCAGAGGCGTTGCCGCTCGGGTCGGTCTGTACCAGGCTCAGGCGTTCACCCGGCAGCGCAGCCGGGTCGAGGTCGATCAGGAAAGTACCGTTCGCGGTCACTGTGCCGGTACCGACCAGCGTGCCATTGGCATc                                                                   <  2:348286/142‑1 (MQ=255)
                                                                              tCAGCGCTTCAGAGGCGTTGCCGCTCGGGTCGGTCTGTACCAGGCTCAGGCGTTCACCCGGCAGCGCAGCCGGGTCGAGGTCGATCAGGAAAGTACCGCTCCCGCAAGATCTGCCCGGACCCACCAGCGCGCCATGTGCATCGCGCACCt                                                           >  2:437618/1‑150 (MQ=255)
                                                                               cAGCGCTTCAGAGGCGTTGCCGCTCGGGTCGGTCTGTACCAGGCTCAGGCGTTCACCCGGCAGCGCAGCCGGGTCGAGGTCGATCAGGAAAGTACCGTTCGCGGTCACTGTGCCGGTACCGACCAGCGTGCCATTgg                                                                       <  2:211824/137‑1 (MQ=255)
                                                                               cAGCGCTTCAGAGGCGTTGCCGCTCGGGTCGGTCTGTACCAGGCTCAGGCGTTCACCCGGCAGCGCAGCCGGGTCGAGGTCGATCAGGAAAGTACCGTTCGCGGTCACTGTGCCGGTACCGACCAGCGTGCCATTgg                                                                       >  1:211824/1‑137 (MQ=255)
                                                                                    cTTCAGAGGCGTTGCCGCTCGGGTCGGTCTGTACCAGGCTCAGGCGTTCACCCGGCAGCGCAGCCGGGTCGAGGTCGATCAGGAAAGTACCGTTCGCGGTCACTGTGCCGGTACCGACCAGCGTGCCATTGGCATCGCGCACCTCGACgg                                                     <  1:463166/150‑1 (MQ=255)
                                                                                                                 tGTACCAGGCTCAGGCGTTCACCCGGCAGCGCAGCCGGGTCGAGGTCGATCAGGAAAGTACCGTTCGCGGTCACTGTGCCGGTACCGACCAGCGTGCCATTGGCATCGCGCACCTCGACGGTAGCACCGGCTTCGCCGCGACCGCCCAAg                        >  1:145952/1‑150 (MQ=255)
                                                                                                                 tGTACCAGGCTCAGGCGTTCACCCGGCAGCGCAGCCGGGTCGAGGTCGATCAGGAAAGTACCGTTCGCGGTCACTGTGCCGGTACCGACCAGCGTGCCATTGGCATCGCGCACCTCGACGGTAGCACCGGCTTCGCCGCGACCGCCCAAg                        <  1:275449/150‑1 (MQ=255)
                                                                                                                     ccAGGCTCAGGCGTTCACCCGGCAGCGCAGCCGGGTCGAGGTCGATCAGGAAAGTACCGTTCGCGGTCACTGTGCCGGTACCGACCAGCGTGCCATTGGCATCGCCCACCTCGACGGTAGCACCGGCTTCGCCGCGACCGCCCAAGGTCa                    <  1:351648/150‑1 (MQ=255)
                                                                                                                     ccAGGCTCAGGCGTTCACCCGGCAGCGCAGCCGGGTCGAGGTCGATCAGGAAAGTACCGTTCGCGGTCACTGTGCCGGTACCGACCAGCGTGCCATTGGCATCACGCACCTCGACGGTAGCACCGGCTTCGCCGCGACCGCCCaa                         <  1:304678/145‑1 (MQ=255)
                                                                                                                     ccAGGCTCAGGCGTTCACCCGGCAGCGCAGCCGGGTCGAGGTCGATCAGGAAAGTACCGTTCGCGGTCACTGTGCCGGTACCGACCAGCGTGCCATTGGCATCACGCACCTCGACGGTAGCACCGGCTTCGCCGCGACCGCCCaa                         >  2:304678/1‑145 (MQ=255)
                                                                                                                           tCAGGCGTTCACCCGGCAGCGCAGCCGGGTCGAGGTCGATCAGGAAAGTACCGTTCGCGGTCACTGTGCCGGTACCGACCAGCGTGCCATTGGCATCGCGCACCTCGACGGTAGCACCGGCTTCGCCGCGACCGCCCAAGGTCAGGCCGt              >  2:98991/1‑150 (MQ=255)
                                                                                                                             aGGCGTTCACCCGGCAGCGCAGCCGGGTCGAGGTCGATCAGGAAAGTACCGTTCGCGGTCACTGTGCCGGTACCGACCAGCGTGCCATTGGCATCGCGCACCTCGACGGt                                                    >  1:80353/1‑110 (MQ=255)
                                                                                                                             aGGCGTTCACCCGGCAGCGCAGCCGGGTCGAGGTCGATCAGGAAAGTACCGTTCGCGGTCACTGTGCCGGTACCGACCAGCGTGCCATTGGCATCGCGCACCTCGACGGt                                                    <  2:80353/110‑1 (MQ=255)
                                                                                                                                 ctcgaCCCGGCTGCGCTGCCGGGTCGAGGTCGATCAGGAAAGTACCGTTCGCGGTCACTGTGCCGGTACCGACCAGCGTGCCATTGGCATCGCGCACCTCGACGGTAGCACCGGc                                           >  2:275739/5‑115 (MQ=255)
                                                                                                                                 ctcgaCCCGGCTGCGCTGCCGGGTCGAGGTCGATCAGGAAAGTACCGTTCGCGGTCACTGTGCCGGTACCGACCAGCGTGCCATTGGCATCGCGCACCTCGACGGTAGCACCGGc                                           <  1:275739/111‑1 (MQ=255)
                                                                                                                                 ctcgaCCCGGCTGCGCTGCCGGGTCGAGGTCGATCAGGAAAGTACCGTTCGCGGTCACTGTGCCGGTACCGACCAGCGTGCCATTGGCATCGCGCACCTCGACGGTAGCACCGGCTTCGCCGCGACCGCCCAAGGTCAGGCCGTCGgcgc        >  1:77949/5‑150 (MQ=255)
                                                                                                                                 ctcgaCCCGGCTGCGCTGCCGGGTCGAGGTCGATCAGGAAAGTACCGTTCGCGGTCACTGTGCCGGTACCGACCAGCGTGCCATTGGCATCGCGCACCTCGACGGTAGCACCGGCTTCGCCGCGACCGCCCAAGGTCAGGCCGTCGgcgc        >  1:77881/5‑150 (MQ=255)
                                                                                                                                     aCCCGTCAGCGCAGCCGGGTCGAGGTCGATCAGGAAAGTACCGTTCGCGGTCACTGTGCCGGTATCGACCAGCGTGCCATTGGCATCGCGCACCTCGACGGTAGCACCGGCTTCGCCGCGACCGCCCAAGGTCAGGCCGTCGTCGCcgac    >  2:213331/1‑150 (MQ=255)
                                                                                                                                       ccGGCAGCGCAGCCGGGTCGAGGTCGATCAGGAAAGTACCGTTCGCGGTCACTGTGCCGGTACCGACCAGCGTGCCATTGGCATCGCGCACCTCGACGGTAGCACCGGCTTCGCCGCGACCGCCCAAGGTCAGGCCGTCGGCGCcgacga  >  1:30184/1‑150 (MQ=255)
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CGCTGATGCTGCTGCCATCGGCAGCGACGACCAGTTCGCTCGGGCTGGTCGGCGTGACCACCAGTGGCACGTCGTAGGTCAGCGCTTCAGAGGCGTTGCCGCTCGGGTCGGTCTGTACCAGGCTCAGGCGTTCACCCGGCAGCGCAGCCGGGTCGAGGTCGATCAGGAAAGTACCGTTCGCGGTCACTGTGCCGGTACCGACCAGCGTGCCATTGGCATCGCGCACCTCGACGGTAGCACCGGCTTCGCCGCGACCGCCCAAGGTCAGGCCGTCGGCGCCGACGA  >  NC_002947/937626‑937910

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑