Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,169,311 T→C 14.0% I369V (ATC→GTC)  leuA ← 2‑isopropylmalate synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,169,3110TC14.0% 98.5 / 8.8 43I369V (ATC→GTC) leuA2‑isopropylmalate synthase
Reads supporting (aligned to +/- strand):  ref base T (18/19);  new base C (3/3);  total (21/22)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.83e-01

TCACCCTGCACCACCTGGCTGAATTCGATCTGCATGCGACGCGGCAGGCTGATGCCATATTCCTGCTCGAGCAGGTAGGTGATACCGCCTTTGCCCGACTGGCTGTTGACGCGGATTACCGCTTCGTAGCTGCGGCCGATGTCGGCCGGGTCGATCGGCAGGTAAGGCACCTCCCACAGTTCCCCATCCTGCTGCTTGGCGAAGCCCTTGCGGATGGCGTCCTGGTGCGAGCCGGAGAACGCGGTATGGACCAGATCCCCCACATAGGGGTGACGAGGATGTACCG  >  NC_002947/1169172‑1169457
                                                                                                                                           |                                                                                                                                                  
ccACCCTGCACCACCTGGCTGAATTCGATCTGCATGCGACGCGGCAGGCTGATGCCATATTCCTGCTCGAGCAGGTAGGTGATACCGCCTTTGCCCGACTGGCTGTTGACGCGGATTACCGCTTCGTAGCTGCGGCCGACATCGGCCgca                                                                                                                                          <  2:316451/149‑3 (MQ=255)
    ccTGCACCACCTGGCTGAATTCGATCTGCATGCGACGCGGCAGGCTGATGCCATATTCCTGCTCGAGCAGGTAGGTGATACCGCCTTTGCCCGACTGGCTGTTGACGCGGATTACCGCTTCGTAGCTGCGGCCGATGTCGGCCGGGTCGa                                                                                                                                      >  2:103064/1‑150 (MQ=255)
    ccTGCACCACCTGGCTGAATTCGATCTGCATGCGACGCGGCAGGCTGATGCCATATTCCTGCTCGAGCAGGTAGGTGATACCGCCTTTGCCCGACTGGCTGCTGACGCGGATTACCGCTTCGTAGCTGCGGCCGATGTCGGCCGGGTCGa                                                                                                                                      >  2:341546/1‑150 (MQ=255)
      tGCACCACCTGGCTGAATTCGATCTGCATGCGACGCGGCAGGCTGATGCCATATTCCTGCTCGAGCAGGTAGGTGATACCGCCTTTGCCCGACTGGCTGTTGACGCGGATTACCGCTTCGTAGCTGCGGCCGATGTCGGCCGGGTCGATc                                                                                                                                    <  1:22660/150‑1 (MQ=255)
              cTGGCTGAATTCGATCTGCATGCGACGCGGCAGGCTGATGCCATATTCCTGCTCGAGCAGGTAGGTGATACCGCCTTTGCCCGACTGGCTGTTGACGCGGATTACCGCTTCGTAGCTGCGGCCGATGTCGGCCGGGTCGATCGGCAGGTa                                                                                                                            >  2:389977/1‑150 (MQ=255)
                 gCTGAATTCGACCTGCATGCGACGCGGCAGGCTGATGCCATATTCCTGCTCGAGCAGGTAGGTGATACCGCCTTTGCCCGACTGGCTGTTGACGCGGATTACCGCTTCGTAGCTGCGGCCGATGTCGGCCGGGTCGATCGGCAGGTAAgg                                                                                                                         <  1:389977/150‑1 (MQ=255)
                 gCTGAATGCGATTCTTATGCGACTCGGCAGGCTGATGCCATATTCCTGCTCGAGCAGGTAGGTGATACCGCCTTTGCCCGACTGGCTGTTGACGCGGATTACCGCTTCGTAGCTGCGGCCGATGTCGGCCGGGTCGATCGGCAGGTAAgg                                                                                                                         <  2:157907/150‑1 (MQ=255)
                    gAATTCGATCTGCATGCGACGCGGCAGGCTGATGCCATATTCCTGCTCGAGCAGGTAGGTGATACCGCCTTTGCCCGACTGGCTGTTGACGCGGATTACCGCTTCGTAGCTGCGGCCGATGTCGGCCGGGTCGATCGGCAGGTAAGGCAc                                                                                                                      <  1:103064/150‑1 (MQ=255)
                      aTTCGATCTGCATGCGACGCGGCAGGCTGATGCCATATTCCTGCTCGAGCAGGTAGGTGATACCGCCTTTGCCCGACTGGCTGTTGACGCGGATTACCGCTTCGTAGCTGCGGCCGATGTCGGCCGGGTCGATCg                                                                                                                                   >  1:77239/1‑135 (MQ=255)
                      aTTCGATCTGCATGCGACGCGGCAGGCTGATGCCATATTCCTGCTCGAGCAGGTAGGTGATACCGCCTTTGCCCGACTGGCTGTTGACGCGGATTACCGCTTCGTAGCTGCGGCCGATGTCGGCCGGGTCGATCg                                                                                                                                   <  2:77239/135‑1 (MQ=255)
                      aTTCGATCTGCATGCGACGCGGCAGGCTGATGCCATATTCCTGCTCGAGCAGGTAGGTGATACCGCCTTTGCCCGACTGGCTGTTGACGCGGATTACCGCTTCGTAGCTGCGGCCGATGTCGGCCGGGTCGATCGGCAGGTAAGGCACCt                                                                                                                    >  2:269352/1‑150 (MQ=255)
                        tCGATCTGCATGCGACGCGGCAGGCTGATGCCATATTCCTGCTCGAGCAGGTAGGTGATACCGCCTTTGCCCGACTGGCTGTTGACGCGGATTACCGCTTCGTAGCTGCGGCCGATGTCGGCCGGGTCGATCGGCAGGTAAGGCACCTcc                                                                                                                  <  1:341546/150‑1 (MQ=255)
                                 aTGCGACGCGGCAGGCTGATGCCATATTCCTGCTCGAGCAGGTAGGTGATACCGCCTTTGCCCGACTGGCTGTTGCCGCGGATTACCGCTTCGTAGCTGCGGCCGATGTCGGCCGGGTCGATCGGCAGGTAAGGCACCTCCCACAGTTcc                                                                                                         <  2:230535/150‑1 (MQ=255)
                                 aTGCGACGCGGCAGGCTGATGCCATATTCCTGCTCGAGCAGGTAGGTGATACCGCCTTTGCCCGACTGGCTGTTGACGCGGATTACCGCTTCGTAGCTGCGGCCGACATCGGCCgca                                                                                                                                          <  2:263303/117‑3 (MQ=255)
                                 aTGCGACGCGGCAGGCTGATGCCATATTCCTGCTCGAGCAGGTAGGTGATACCGCCTTTGCCCGACTGGCTGTTGACGCGGATTACCGCTTCGTAGCTGCGGCCGACATCGGCCgca                                                                                                                                          >  1:263303/1‑115 (MQ=255)
                                  tGCGACGCGGCAGGCTGATGCCATATTCCTGCTCGAGCAGGTAGGTGATACCGCCTTTGCCCGACTGGCTGTTGACGCGGATTACCGCTTCGTAGCTGCGGCCGATGTCGGCCGGGTCGATCGGCAGGTAAGGCACCTCCCACAGTTccc                                                                                                        >  1:213602/1‑150 (MQ=255)
                                             aGGCTGATGCCATATTCCTGCTCGAGCAGGTAGGTGATACCGCCTTTGCCCGACTGGCTGTTGACGCGGATTACCGCTTCGTAGCTGCGGCCGATGTCGGCCGGGTCGATCGGCAGGTAAGGCACCTCCCACAGTTCCCCATCCtgctgc                                                                                             <  1:269352/150‑1 (MQ=255)
                                                              cTGCTCGAGCAGGTAGGTGATACCGCCTTTGCCCGACTGGCTGTTGACGCGGATTACCGCTTCGTAGCTGCGGCCGATGTCGGCCGGGTCGATCGGCAGGTAAGGCACCTCCCACAGTTCCCCATCCTGCTGCTTGGCGAAGCCCTTGCg                                                                            >  1:350893/1‑150 (MQ=255)
                                                              cTGCTCGAGCAGGTAGGTGATACCGCCTTTGCCCGACTGGCTGTTGACGCGGATTACCGCTTCGTAGCTGCGGCCGATGTCGGCCGGGTCGATCGGCAGGTAAGGCACCTCCCACAGTTCCCCATCCTGCTGCTTGGCGAAGCCCTTGCg                                                                            >  2:39983/1‑150 (MQ=255)
                                                                                    ccGCCTTTGCCCGACTGGCTGTTGACGCGGATTACCGCTTCGTAGCTGCGGCCGATGTCGGCCGGGTCGATc                                                                                                                                    >  2:334491/1‑72 (MQ=255)
                                                                                    ccGCCTTTGCCCGACTGGCTGTTGACGCGGATTACCGCTTCGTAGCTGCGGCCGATGTCGGCCGGGTCGATc                                                                                                                                    <  1:334491/72‑1 (MQ=255)
                                                                                       ccTTTGCCCGACTGGCTGTTGACGCGGATTACCGCTTCGTAGCTGCGGCCGATGTCGGCCGGGTCGATCGGCAGGTAAGGCACCTCCCACAGTTCCCCATCCTGCTGCTTGGCGAAGCCCTTGCGGATGGCGTCCTGGTGCGAGCCGgag                                                   >  1:406654/1‑150 (MQ=255)
                                                                                           tGCCCGACTGGCTGTTGACGCGGATTACCGCTTCGTAGCTGCGGCCGATGTCGGCCGGGTCGATCGGCAGGTAAGGCACCTCCCACAGTTCCCCATCCTGCTGCTTGGCGAAGCCCTTGCGGATGGCGTCCTGGTGCGAGCCGGAGAAcg                                               <  2:350893/150‑1 (MQ=255)
                                                                                             cccGACTGGCTGTTGACGCGGATTACCGCTTCGTAGCTGCGGCCGATGTCGGCCGGGTCGATCGGCAGGTAAGGCACCTCCCACAGTTCCCCATCCTGCTGCTTGGCGAAGCCCTTGCGGATGGCGTCCTGGTGCGAGCCGGAGAAcgcg                                             >  1:215063/1‑150 (MQ=255)
                                                                                              ccGACTGGCTGTTGACGCGGATTACCGCTTCGTAGTTGCGGCCGATGTCGGCCGGGTCGATCGGCAGGTAAGGCACCTCCCACAGTTCCCCATCCTGCTGCTTGGCGGAGc                                                                                   <  2:335674/111‑1 (MQ=255)
                                                                                              ccGACTGGCTGTTGACGCGGATTACCGCTTCGTAGTTGCGGCCGATGTCGGCCGGGTCGATCGGCAGGTAAGGCACCTCCCACAGTTCCCCATCCTGCTGCTTGGCGGAGc                                                                                   >  1:335674/1‑111 (MQ=255)
                                                                                                    ggCTGTTGACGCGGATTACCGTTTCGTAGCTGCGGCCGATGTCGGCCGGGTCGATCGGCAGGTAAGGCACCTCCCACAGTTCCCCATCCTGCTGCTTGGCGAAGCCCTTGCGGATGGCGTCCTGGTGCGAGCCGGAGAACGCGGTATGGa                                      <  2:213602/150‑1 (MQ=255)
                                                                                                         ttGACGCGGATTACCGCTTCGTAGCTGCGGCCGATGTCGGCCGGGTCGATCGGCAGGTAAGGCACCTCCCACAGTTCCCCATCCTGCTGCTTGGCGAAGCCCTTGCGGATGGCGTCCTGGTGCGAGCCGGAGAACGCGGTATGGACCTGa                                 <  1:508669/150‑1 (MQ=255)
                                                                                                              gcgGATTACCGCTTCGTAGCTGCGGCCGATGTCGGCCGGGTCGATCGGCAGGTAAGGCACCTCCCACAGTTCCCCATCCTGCTGCTTGGCGAAGCCCTTGCGGATGGCGTCCTg                                                                >  2:474408/1‑114 (MQ=255)
                                                                                                              gcgGATTACCGCTTCGTAGCTGCGGCCGATGTCGGCCGGGTCGATCGGCAGGTAAGGCACCTCCCACAGTTCCCCATCCTGCTGCTTGGCGAAGCCCTTGCGGATGGCGTCCTg                                                                <  1:474408/114‑1 (MQ=255)
                                                                                                              gcgGATTACCGCTTCGTAGCTGCGGCCGATGTCGGCCGGGTCGATCGGCAGGTAAGGCACCTCCCACAGTTCCCCATCCTGCTGCTTGGCGAAGCCCTTGCGGATGGCGTCCTGGTGCGAGCCGGAGAACGCGGTATGGACCAGATcccc                            >  1:252208/1‑150 (MQ=255)
                                                                                                              gcgGATTACCGCTTCGTAGCTGCGGCCGATGTCGGCCGGGTCGATCGGCAGGTAAGGCACCTCCCACAGTTCCCCATCCTGCTGCTTGGCGAAGCCCTTGCGGATGGCGTCCTGGTGCGAGCCGGAGAACGCGGTATGGACCAGATcccc                            <  2:406654/150‑1 (MQ=255)
                                                                                                                      ccGCTTCGTAGCTGCGGCCGATGTCGGCCGGGTCGATCGGCAGGTAAGGCACCTCCCACAGTTCCCCATCCTGCTGCTTGGCGAAGCCCTTGCGGATGGCGTCCTGGTGCGAGCCGGAGAACGCGGTATGGACCAGATCCCCCACATAgg                    >  2:58085/1‑150 (MQ=255)
                                                                                                                             gTAGCTGCGGCCGATGTCGGCCGGGTCGATCGGCAGGTAAGGCACCTCCCACAGTTCCCCATCCTGCTGCTTGGCGAAGCCCTTGCGGATGGCGTCCTGGTGCGa                                                          >  1:164349/1‑105 (MQ=255)
                                                                                                                             gTAGCTGCGGCCGATGTCGGCCGGGTCGATCGGCAGGTAAGGCACCTCCCACAGTTCCCCATCCTGCTGCTTGGCGAAGCCCTTGCGGATGGCGTCCTGGTGCGa                                                          <  2:164349/105‑1 (MQ=255)
                                                                                                                                gCTGCGGCCGATGTCGGCCGGGTCGATCGGCAGGTAAGGCACCTCCCACAGTTCCCCATCCTGCTGCTTGGCGAAGCCCTTGCGGATg                                                                        >  2:322955/1‑88 (MQ=255)
                                                                                                                                gCTGCGGCCGATGTCGGCCGGGTCGATCGGCAGGTAAGGCACCTCCCACAGTTCCCCATCCTGCTGCTTGGCGAAGCCCTTGCGGATg                                                                        <  1:322955/88‑1 (MQ=255)
                                                                                                                                 acccGGCCGACATCGGCCGGGTCGATCGGCAGGTAAGGCACCTCCCACAGTTCCCCATCCTGCTGCTTGGCGAAGCCCTTGCGGATGGCGTCCTGGTGCGAGCCGGAGAACGCGGTATGGACCAGATCCCCCACATAGGGGTGACGAGGa         >  1:48277/4‑150 (MQ=255)
                                                                                                                                   ccGGCCGACATCGGCCGGGTCGATCGGCAGGTAAGGCACCTCCCACAGTTCCCCATCCTGCTGCTTGGCGAAGCCCTTGCGGATGGCGTCCTGGTGCGAGCCGGAGAACGCGGTATGGa                                      <  1:229063/118‑1 (MQ=255)
                                                                                                                                   ccGGCCGACATCGGCCGGGTCGATCGGCAGGTAAGGCACCTCCCACAGTTCCCCATCCTGCTGCTTGGCGAAGCCCTTGCGGATGGCGTCCTGGTGCGAGCCGGAGAACGCGGTATGGa                                      >  2:229063/2‑119 (MQ=255)
                                                                                                                                       ccGATGTCGGCCGGGTCGATCGGCAGGTAAGGCACCTCCCACAGTTCCCCATCCTGCTGCTTGGCGAAGCCCTTGCGGATGGCGTCCTGGTGCGAGCCGGAGAACGCGGTATGGACCAGATCCCCCACATAggg                   <  2:354038/134‑1 (MQ=255)
                                                                                                                                       ccGATGTCGGCCGGGTCGATCGGCAGGTAAGGCACCTCCCACAGTTCCCCATCCTGCTGCTTGGCGAAGCCCTTGCGGATGGCGTCCTGGTGCGAGCCGGAGAACGCGGTATGGACCAGATCCCCCACATAggg                   >  1:354038/1‑134 (MQ=255)
                                                                                                                                        cGATGTCGGCCGGGTCGATCGGCAGGTAAGGCACCTCCCACAGTTCCCCATCCTGCTGCTTGGCGAAGCCCTTGCGGATGGCGTCCTGGTGCGAGCCGGAGAACGCGGTATGGACCAGATCCCCCACATAGGGGTGACGAGGATGTACCg  <  1:58085/150‑1 (MQ=255)
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TCACCCTGCACCACCTGGCTGAATTCGATCTGCATGCGACGCGGCAGGCTGATGCCATATTCCTGCTCGAGCAGGTAGGTGATACCGCCTTTGCCCGACTGGCTGTTGACGCGGATTACCGCTTCGTAGCTGCGGCCGATGTCGGCCGGGTCGATCGGCAGGTAAGGCACCTCCCACAGTTCCCCATCCTGCTGCTTGGCGAAGCCCTTGCGGATGGCGTCCTGGTGCGAGCCGGAGAACGCGGTATGGACCAGATCCCCCACATAGGGGTGACGAGGATGTACCG  >  NC_002947/1169172‑1169457

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑