| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 1,362,915 | T→A | 12.1% | D433E (GAT→GAA) | phoQ → | sensor protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,362,915 | 0 | T | A | 12.1% | 79.8 / 5.8 | 33 | D433E (GAT→GAA) | phoQ | sensor protein |
| Reads supporting (aligned to +/- strand): ref base T (10/19); new base A (2/2); total (12/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.10e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.43e-01 | |||||||||||
GACGACGGGCCGGGCGTGCCCGTCGACCAGCGTGAACGCATTCTGGAGCGTGGGGAGCGGCTGGACAGCCAGCACCCGGGGCAGGGCATCGGCTTGGCAGTGGTCAAGGATATCGTCGACAGCTATGACGCCGAGCTGAGCCTTGGCGATTCGCCACTGGGGGGCGCTGCGTTCAGGATCACCTTCCACCTGGATTGATTCTTAGCCTGTGCCAGCCCGTTCGCAGGTCAATCCGCGAATGGGCCGGCACAGGCAATCCACAAAGGGCGGATTTCCGCCATTCCTCCTTGTACAA > NC_002947/1362766‑1363060 | gacgacGGGCCGGGCGTGCCCGTCGACCAGCGTGAACGCATTCTGGAGCGTGGGGAGCGGCTGGACAGCCAGCACCCGGGGCAGGGCATCGGCTTGGCAGTGGTCAAGGATATCGTCGACAGCTATGACGCCGAGCTGAGCCTTGGCGAt > 2:207298/1‑150 (MQ=255) gacgGGCCGGGCGTGCCCGTCGACCAGCGTGAACGCATTCTGGAGCGTGGGGAGCGGCTGGACCGCCAGCACCCGGGGCAGGGCATCGGCTTGGCAGTGGTCAAGGATATCGTCGACAGCTATGACGCCGAGCTGAGCCTTGGCGATTCg > 2:477168/1‑150 (MQ=255) gacgGGCCGGGCGTGCCCGTCGACCAGCGTGAACGCATTCTGGAGCGTGGGGAGCGGCTGGACAGCCAGCACCCGGGGCAGGGCATCGGCTTGGCAGTGGTCAAGGATATCGTCGACAGCTATGACGCCGAGCTGAGCCTTGGCGATTCg > 2:350666/1‑150 (MQ=255) gggcGTGCCCGTCGACCAGCGTGAACGCATTCTGGAGCGTGGGGAGCGGCTGGACAGCCAGCACCCGGGGCAGGGCATCGGCTTGGCAGTGGTCAAGGATATCGTCGACAGCTATGACGCCGAGCTGAGCCTTGGCGATTCGCCACTggg < 1:207298/150‑1 (MQ=255) cGTCGACCAGCGTGAACGCATTCTGGAGCGTGGGGAGCGGCTGGACAGCCAGCACCCGGGGCAGGGCATCGGCTTGGCAGTGGTCAAGGATATCGTCGACAGCTATGACGCCGAGCTGAGCCTTGGCGATTCGCCACTGGGGGGCGCTGc > 2:200876/1‑150 (MQ=255) ttCTGGAGCGTGGGGAGCGGCTGGACAGCCAGCACCCGGGGCAGGGCATCGGCTTGGCAGTGGTCAAGGATATCGTCGACAGCTATGACGCCGAGCTGAGCCTTGGCGATTCGCCACTGGGGGGCGCTGCGTTCAGGATCACCTTCCAcc > 2:285292/1‑150 (MQ=255) ttCTGGAGCGTGGGGAGCGGCTGGACAGCCAGCACCCGGGGCAGGGCATCGGCTTGGCAGTGGTCAAGGATATCGTCGACAGCTATGACGCCGAGCTGAGCCTTGGCGATTCCCAACTGGGGGGCGCTGCGTTCAGGATCACCTTCCAcc < 1:40449/150‑1 (MQ=255) tGGAGCGTGGGGAGCGGCTGGACAGCCAGCACCCGGGGCAGGGCATCGGCTTGGCAGTGGTCAAGGATATCGTCGACAGCTATGACGCCGAGCTGAGCCTTGGCGATTCGCCACTGGGGGGCGCTGCGTTCAGGATCACCTTCCACCTgg > 2:14792/1‑150 (MQ=255) gAGCGTGGGGAGCGGCTGGACAGCCAGCACCCGGGGCAGGGCATCGGCTTGGCAGTGGTCAAGGATATCGTCGACAGCTATGACGCCGAGCTGAGCCTTGGCGATTCGCCACTGGGGGGCGCTGCGTTCAGGATCACCTTCCACCTGgat < 1:63036/150‑1 (MQ=255) gTGGGGAGCGGCTGGACAGCCAGCACCCGGGGCAGGGCATCGGCTTGGCAGTGGTCAAGGATATCGTCGACAGCTATGACGCCGAGCTGAGCCTTGGCGATTCGCCACTGGGGGGCGCTGCGTTCAGGATCACCTTCCACCTGgattgat < 1:205640/150‑1 (MQ=255) gCTGGGCAGCCAGCACCCGGGGCAGGGCATCGGCTTGGCAGTGGTCAAGGATATCGTCGACAGCTATGACGCCGAGCTGAGCCTTGGCGATTCGCCACTGGGGGGCGCTGCGTTCAGGATCACCTTCCACCTGGATTGATTCTTAGCCtg > 2:218572/1‑150 (MQ=255) gCTGGACAGCCAGCACCCGGGGCAGGGCATCGGCTTGGCAGTGGTCAAGGATATCGTCGACAGCTATGACGCCGAGCTGAGCCTTGGCGATTCGCCACTGGGGGGCGCTGCGTTCAGGATCACCTTCCACCTGGATTGATTCTTAGCCtg > 1:101516/1‑150 (MQ=255) cagccagcACCCGGGGCAGGGCATCGGCTTGGCAGTGGTCAAGGATATCGTCGACAGCTATGACGCCGAGCTGAGCCTTGGCGATTCGCCACTGGGGGGCGCTGCGTTCAGGATCACCTTCCACCTGGATTGATTCTTAGCCTGTGCCAg < 1:200876/150‑1 (MQ=255) cgccagcACCCGGGGCAGGGCATCGGCTTGGCAGTGGTCAAGGATATCGTCGACAGCTATGACGCCGAGCTGAGCCTTGGCGATTCGCCACTGGGGGGCGCTGCGTTCAGGATCACCTTCCACCTGGATTGATTCTTAGCCTGTGCCAGc < 1:262901/149‑1 (MQ=255) agccagcACCCGGGGCAGGGCATCGGCTTGGCAGTGGTCAAGGATATCGTCGACAGCTATGACGCCGAGCTGAGCCTTGGCGAATCGCCaagg < 1:136195/93‑4 (MQ=255) agccagcACCCGAGGCAGGGCATCGGCTTGGCAGTGGTCAAGGATATCGTCGACAGCTATGACGCCGAGCTGAGCCTTGGCGAATCGCCaagg > 2:136195/1‑90 (MQ=255) ccagcACCCGGGGCAGGGCATCGGCTTGGCAGTGGTCAAGGATATCGTCGACAGCTATGACGCCGAGCTGAGCCTTGGCGATTCGCCACTGGGGGGAGCTGCGGTCAGGATCACCTTCCACCTGGATTGATTCTTAGCCTGTGCCAGccc > 1:111547/1‑150 (MQ=255) cccGGGGCAGGGCATCGGCTTGGCAGTGGTCAAGGATATCGTCGACAGCTATGACGCCGAGCTGAGCCTTGGCGATTCGCCACTGGGGGGCGCTGCGTTCAGGATCACCTTCCACCTGGATTGATTCTTAGCCTGTGCCAGCCCGTTCGc > 2:81762/1‑150 (MQ=255) cGGGGCAGGGCATCCGCTTGGCAGTGGTCAAGGATATCGACGACAGCTATGACGCCGAGCTGAGCCTTGGCGATTCGCCACTGGGGGGCGCTGCGTTCAGGATCACCTTCCACCTGGATTGATTCTTAGCCGGTCCCAGCCCGTGCGCAg < 2:70000/150‑1 (MQ=255) ggcaTCGGCTTGGCAGTGGTCAAGGATATCGTCGACAGCTATGACGCCGAGCTGAGCCTTGGCGATTCGCCACTGGGGGGCGCTGCGTTCAGGATCACCTTCCACCTGGATTGATTCTTAGCCTGTGCCAGCCCGTTCGCAGGTCAATcc < 1:477168/150‑1 (MQ=255) cGGCTTGGCAGTGGTCAAGGATATCGTCGACAGCTATGACGCCGAGCTGAGCCTTGGCGATTCGCCACTGGGGGGCGCTGCGTTCAGGATCACCTTCCACCTGGATTGATTCTTAGCCTGTGCCAGCCCGTTCGCAGGTCAATCCGCGaa < 1:14792/150‑1 (MQ=255) ggCTTGGCAGTGGTCAAGGATATCGTCGACAGCTATGACGCCGAGCTGAGCCTTGGCGATTCGCCACTGGGGGGCGCTGCGTTCAGGATCACCTTCCACCTGGATTGATTCTTAGCCTGTGCCAGCCCGTTCGCAGGTCAATCCGCGAAt < 1:285292/150‑1 (MQ=255) ggCAGTGGTCAAGGATATCGTCGACAGCTATGACGCCGAGCTGAGCCTTGGCGATTCGCCACTGGGGGGCGCTGCGTTCAGGATCACCTTCCACCTGGATTGATTCTTAGCCTGTGCCAGCCCGTTCGCAGGTCAATCCGCGAATGGGcc < 1:81762/150‑1 (MQ=255) gCAGTGGGGAAGTATAACGTCGACAGCTATGACGCCGAGCTGAGCCTTGGCGATTCGCCACTGGGGGGCGCTGCGTTCAGGATCCCCTTCCACCTGGATTGATTCTTAGCCTGTGCCAGACCGTTCGCAGGTCAATCCGCGAATGGGCCg < 2:101516/150‑1 (MQ=255) gTCAAGGATATCGTCGACAGCTATGACGCCGAGCTGAGCCTTGGCGATTCGCCACTGGGGGGCGCTGCGTTCAGGATCACCTTCCACCTGGATTGATTCTTAGCCTGTGCCAGCCCGTTCGCAGGTCAATCCGCGAATGGGCCGGCACAg > 1:334864/1‑150 (MQ=255) gATATCGTCGACAGCTATGACGCCGAGCTGAGCCTTGGCGATTCGCCACTGGGGGGCGCTGCGTTCAGGATCACCTTCCACCTGGATTGATTCTTAGCCTGTGCCAGCCCGTTCGCAGGTCAATCCGCGAATGGGCCGGCACAGGCAATc < 2:442660/150‑1 (MQ=255) cgACAGCTATGACGCCGAGCTGAGCCTTGGTGATTCGCCACTGGGGGGCGCTGCGTTCAGGATCACCTTCCACCTGGATTGATTCGTAGCCTGTGCCAGCACGTTCGCAGGTTAATCCGCGAATGGGCCGGCACACGCAATCCACAAAgg < 1:132368/150‑1 (MQ=255) cGCCGAGCTGAGCCTTGGCGATTCGCCACTGGGGGGCGCTGCGTTCAGGATCACCTTCCACCTGGATTGATTCTTAGCCTGTGCCAGCCCGTTCGCAGGTCAATCCGCGAATGGGCCGGCACAGGCAATCCACAAAGGGCGGATTTCCGc < 1:225352/150‑1 (MQ=255) gAGCCTTGGCGATTCGCCACTGGGGGGCGCTGCGTTCAGGATCACCTTGCACCTGGATTGATTCTTAGCCTGTGCCAGCCCGTTCGCAGGTCAATCCGCGAATGGGCCGGCACAGGCAATCCACAAAGGGCGGATTTCCGCCATtcctcc < 1:206400/150‑1 (MQ=255) gAGCCTTGGCGATTCGCCACTGGGGGGCGCTGCGTTCAGGATCACCTTCCACCTGGATTGATTCTTAGCCTGTGCCAGCCCGTTCGCAGGTCAATCCGCGAATGGGCCGGCACAGGCAATCCACAAAGGGCGGATTTCCGCCATtcctcc < 1:218572/150‑1 (MQ=255) cccagtGGCGAATCGCCACTGGGGGGCGCTGCGTTCAGGATCACCTTCCACCTGGATTGATTCTTAGCCTGTGCCAGCCCGTTCGCAGGTCAATCCGCGAATGGGCCGGCACAGGCAATCCACAAAGGGCGGATTTCCGCCATTcctcct > 1:174004/6‑150 (MQ=255) cTTGGCGATTCGCCACTGGGGGGCGATGCGTTCAGGATCACCTTCCACCTGGATTGATTCTTAGCCTGTGCCAGCCCGTTCGCAGGTCAATCCGCGAATGGGCCGGCACAGGCAATCCACAAAGGGCGGATTTCCGCCATTCCTCCTTGt < 1:350666/150‑1 (MQ=255) tGGCGATTCCCCACTCGGGGGCGCGGCGGTCAGGATCACCTTCCACCTGGATTGATTCCTAGCCTGTGCCAGCCCGTTCGCAGGTCAATCCGCGAATGGGCNGGCNCAGGCAATCCACAAAGGGCGGATTTCCGCCATTCCTCCTTGTAc < 1:325042/150‑1 (MQ=255) gCGAATCGCCACTGGGGGGCGCTGCGTTCAGGATCACCTTCCACCTGGATTGATTCTTAGCCTGTGCCAGCCCGTTCGCAGGTCAATCCGCGAATGGGCCTGCACAGGCAATCCACAAAGGGCGGATTTCCGCCATTCCTCCTTGTACaa < 2:174004/150‑1 (MQ=255) | GACGACGGGCCGGGCGTGCCCGTCGACCAGCGTGAACGCATTCTGGAGCGTGGGGAGCGGCTGGACAGCCAGCACCCGGGGCAGGGCATCGGCTTGGCAGTGGTCAAGGATATCGTCGACAGCTATGACGCCGAGCTGAGCCTTGGCGATTCGCCACTGGGGGGCGCTGCGTTCAGGATCACCTTCCACCTGGATTGATTCTTAGCCTGTGCCAGCCCGTTCGCAGGTCAATCCGCGAATGGGCCGGCACAGGCAATCCACAAAGGGCGGATTTCCGCCATTCCTCCTTGTACAA > NC_002947/1362766‑1363060 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |