| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 1,435,822 | A→T | 28.1% | L50Q (CTG→CAG) | PP_1256 ← | alpha‑ketoglutarate semialdehyde dehydrogenase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,435,822 | 0 | A | T | 28.1% | 33.2 / 15.9 | 25 | L50Q (CTG→CAG) | PP_1256 | alpha‑ketoglutarate semialdehyde dehydrogenase |
| Reads supporting (aligned to +/- strand): ref base A (9/9); new base T (4/3); total (13/12) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.56e-01 | |||||||||||
GCCTTGGGCAGGCCGGTTTCGATCACGGCGCGGTCGATCAGGGCATCGCCGAGGGCCTCGATCTGTGTGGCGATGGCTTCAAGGAACTCTGCTCGTTGTTCGAGCGATGTTTCGCGGTAGGCATCGAATGCCGCCCAGGCCAGCGCGCAGGCCTGGGCCACGTGTTCGCCTGTGCCGCCGAGGTAGGCCGGCTCCAACGTCTGACCGGTGGTTGGGTCGATGGCACGGATGGCGTCGCGGCTGCCGGTTACCGGCCTCTGGCCGATCAGCAGGTTGCCTGTGAGG > NC_002947/1435681‑1435965 | gcCTTGGGCAGGCCGGTTTCGATCACGGCGCGGTCGATCAGGGCATCGCCGAGGGCCTCGATCTGTGTGGCGATGGCTTCAAGGAACTCTGCTCGGTGACCGAGCGATGTTTCGCGGTAGGCATCGAATGCCGCCCAGGCCAGCGCGCAg > 2:395614/1‑150 (MQ=255) ccTTGGGCAGGCCGGTTTCGATCACGGCGCGGTCGATCAGGGCATCGCCGAGGGCCTCGATCTGTGTGGCGATGGCTTCAAGGAACTCTGCTCGTTGTTCGAGCGATGTTTCGCGGTAAGCATCGAATGCCGCCCAGGCCAGCGCGCAgg > 2:151274/1‑150 (MQ=255) tGGGCAGGCCGGTTTCGATCACGGCGCGGTCGATCAGGGCATCGCCGAGGGCCTCGATCTGTGTGGCGATGGCTTCAAGGAACTCTGCTCGTTGTTCGAGCGATGTTTCGCGGTAGGCATCGAATGCCGCCCGGGCCAGCGCGCAGGCCt < 2:379362/150‑1 (MQ=255) gCAGGCCGGTTTCGATCACGGCGCGGTCGATCAGGGCATCGCCGAGGGCCTCGATCTGTGTGGCGATGGCTTCAAGGAACTCTGCTCGTTGTTCGAGCGATGTTTCGCGGTAGGCATCGAATGCCGCCCAGGCCTGCGCGCTGGCCTggg < 1:2382/150‑1 (MQ=255) gATCAGGGCATCGCCGAGGGCCTCGATCTGTGTGGCGATGGCTTCAAGGAACTCTGCTCGTTGTTCGAGCGATGTTTCGCGGTAGGCATCGAATGCCGCCCAGGCCAGCGCGCAGGCCTGGGCCACGTGTTCGCCTGTGCCGCCGAGGTa > 1:291951/1‑150 (MQ=255) aGGGCCTCGATCTGTGTGGCGATGGCTTCAAGGAACTCTGCTCGTTGTTCGAGCGATGTTTCGCGGTAAGCATCGAATGCCGCCCAGGCCAGCGCGCAGGCCTGGGCCACGTGTTCGCCTGTGCCGCCGAGGTAGGCCGGCTCCAACGTc < 1:151274/150‑1 (MQ=255) gCCTCGATCTGTGTGGCGATGGCTTCAAGGAACTCTGCTCGTTGTTCGAGCGATGTTTCGCGGTAGGCATCGAATGCCGCCCAGGCCAGCGCGCAGGCCTgg > 1:320073/1‑102 (MQ=255) gCCTCGATCTGTGTGGCGATGGCTTCAAGGAACTCTGCTCGTTGTTCGAGCGATGTTTCGCGGTAGGCATCGAATGCCGCCCAGGCCAGCGCGCAGGCCTgg < 2:320073/102‑1 (MQ=255) ccTCGATCTGTGTGGCGATGGCTTCAAGGAACTCTGCTCGTTGTTCGAGCGATGTTTCGCGGTAGGCATCGAATGCCGCCCAGGCCAGCGCGCAGGCCTGGGCCACGTGTTCGCCTGTGCCGCCGAGGTAGGCCGGCTCCAACGTCTGAc < 1:420732/150‑1 (MQ=255) gATCTGTGTGGCGATGGCTTCAAGGAACTCTGCTCGTTGTTCGAGCGATGTTTCGCGGTAGGCATCGAATGCCGCCCAGGCCAGCGCGCAGGCCTGGGCCACGTGTTCGCCTGTGCCGCCGAGGTAGGCCGGCTCCAACGTCTGACCggt < 2:291951/150‑1 (MQ=255) tgtgGCGATGGCTTCCAGCAACTTTGCCCCTAGTACGATCGATGCCTCGCGGTAGGCATCGAATGCCGCCCAGGCCAGCGCGCAGGCCTGGGCCACGTGTTCGCCTGTGCCGCCGAGGTAGGCCGGCTCCAACGTCTGACCGGTGGTTgg < 2:133681/150‑1 (MQ=255) ttCAAGGAACTCTGCTCGTTGTTCGAGCGATGTTTCGCGGTAGGCATCGAATGCCGCCCAGGCCAGCGCGCAGGCCTGGGCCACGTGTTCGCCTGTGCCGCCGAGGTAGGCCGGCTCCAACGTCTGACCGGTGGTTGGGTCGATGGCACg > 1:53165/1‑150 (MQ=255) aaGGAACTCTGCTCGTTGTTCGAGCGATGTTTCGCGGTAGGCATCGAATGCCGCCCAGGCCAGCGCGCAGGCCTGGGCCACGTGTTCGCCTGTGCCGCCGAGGTAGGCCGGCTCCAACGTCTGACCGGTGGTTGGGTCGATGGCACGGAt > 2:468607/1‑150 (MQ=255) aGGAACTCTGCTCGTTGTTCGAGCGATGTTTCGCGGTAGGCATCGAATGCCGCCCAGGCCAGCGCGCAGGCCTGGGCCACGTGTTCGCCTGTGCCGCCGAGGTAGGCCGGCTCCAACGTCTGACCGGTGGTTGGGTCGATGGCACGGATg < 1:410577/150‑1 (MQ=255) aGGAACTCTGCTCGTTGTTCGAGCGATGGTTCGCGGTACGCATCTAATGCCGCCCAGGCCAGCGCGCAGGCCTGTGCCCCGTGTTCCCCTGTACCGCCGAGGTAGTCCGGCTCCCACCTCTGACCGGCGGTTGGGTCCATGGCACGGAtc > 2:64694/1‑149 (MQ=255) gAACTCTGCTCGTTGTTCGAGCGATGTTTCGCGGTAGGCATCGAATGCCGCCCAGGCCAGCGCGCAGGCCTGGGCCACGTGTTCGCCTGTGCCGCCGAGGTAGGCCGGCTCCAACGTCTGACCGGTGGTTGGGTCGATGGCACGGATGGc < 2:497662/150‑1 (MQ=255) tctGCTCGTTGTTCGAGCGATGTTTCGCGGTAGGCATCGAATGCCGCCCAGGCCAGCGCGCAGGCCTGGGCCACGTGTTCGCCTGTGCCGCCGAGGTAGGCCGGCTCCAACGTCTGACCGGTGGTTGGGTCGATGGCACGGATGGCGTcg > 1:225182/1‑150 (MQ=255) cATCGACTGCCGCCCAGGCCAGCGCGCAGGACTGGGCCACGTGTTCGCCTGTGCCGCCGAGGTAGGCCGGCTCCAACGTCTGACCGGTGGTTGGGTCGATGGCACGGATGGCGTCGCGGCTGCCGGTTACCGGCCTCTGGCCGATcagca < 2:491086/150‑1 (MQ=255) aaTGCCGCCCAGGCCAGCGCGCAGGCCTGGGCCACGTGTTCGCCTGTGCCGCCGAGGTAGGCCGGCTCCAACGTCTGACCGGTGGTTGGGTCGATGGCACGGATGGCGTCGCGGCTGCCGGTTACCGGCCTCTGGCCGATCAGCAGGTTg > 2:472069/1‑150 (MQ=255) cccAGGCCTGCGCGCTGGCCTGGGCCACGTGTTCGCCTGTGCCGCCGAGGTAGGCCGGCTCCAACGTCTGACCGGTGGTTgg < 1:453961/82‑1 (MQ=255) cccAGGCCTGCGCGCTGGCCTGGGCCACGTGTTCGCCTGTGCCGCCGAGGTAGGCCGGCTCCAACGTCTGACCGGTGGTTgg > 2:453961/1‑82 (MQ=255) ccAGGCCTGCGCGCTGGCCTGGGCCACGTGTTCGCCTGTGCCGCCGAGGTAGGCCGGCTCCAACGTCTGACCGGTGGTTGGGTCGATGGCACGGATGGCGTCGCGGCTGCCGGTTACCGGCCTCTGGCCGATcagcag < 2:148601/138‑1 (MQ=255) ccAGGCCTGCGCGCTGGCCTGGGCCACGTGTTCGCCTGTGCCGCCGAGGTAGGCCGGCTCCAACGTCTGACCGGTGGTTGGGTCGATGGCACGGATGGCGTCGCGGCTGCCGGTTACCGGCCTCTGGCCGATcagcag > 1:148601/1‑138 (MQ=255) ccAGGCCTGCGCGCTGGCCTGGGCCACGTGTTCGCCTGTGCCGCCGAGGTAGGCCGGCTCCAACGTCTGACCGGTGGTTGGGTCGATGGCACGGATGACGTCGCGGCTGCCGGTTACCGGCCTCTGGCCGATCAGCAGGTTGCCTGTGAg > 1:348724/1‑150 (MQ=255) cAGGCCTGCGCGCTGGCCTGGGCCACGTGTTCGCCTGTGCCGCCGAGGTAGGCCGGCTCCAACGTCTGACCGGTGGTTGGGTCGATGGCACGGATGGCGTCGCGGCTGCCGGTTACCGGCCTCTGGCCGATCAGCAGGTTGCCTGTGAgg > 2:193999/1‑150 (MQ=255) | GCCTTGGGCAGGCCGGTTTCGATCACGGCGCGGTCGATCAGGGCATCGCCGAGGGCCTCGATCTGTGTGGCGATGGCTTCAAGGAACTCTGCTCGTTGTTCGAGCGATGTTTCGCGGTAGGCATCGAATGCCGCCCAGGCCAGCGCGCAGGCCTGGGCCACGTGTTCGCCTGTGCCGCCGAGGTAGGCCGGCTCCAACGTCTGACCGGTGGTTGGGTCGATGGCACGGATGGCGTCGCGGCTGCCGGTTACCGGCCTCTGGCCGATCAGCAGGTTGCCTGTGAGG > NC_002947/1435681‑1435965 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |