Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,606,449 A→T 16.1% intergenic (‑157/+54) PP_1407 ← / ← PP_t64 sulfate transporter/tRNA‑Gly

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,606,4490AT16.1% 76.3 / 8.8 31intergenic (‑157/+54)PP_1407/PP_t64sulfate transporter/tRNA‑Gly
Reads supporting (aligned to +/- strand):  ref base A (12/14);  new base T (3/2);  total (15/16)
Fisher's exact test for biased strand distribution p-value = 6.54e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.88e-01

TTTCGAATAGTTTCACGAATCCAGTGAGCATATCCGCCACAAGCCGTTTCGCTTGCGCACGCAAAGGCCCGATGCGCATTGAAGCGGATCGGTGGGTAATTGGCCATGGGCCAGGTCAAGGCAGGATGAAAAACGAAAAAAGGAGCCAAGGCTCCTTTTGACTGCGATGACGTTGCTGGAACGCCATGCGGGACTGCGATGTGGAGCGGGTAGAGGGAATCGAACCCTCAACTAAAGCTTGGGAAGCTTTCGTTTTGCCACTAAACTATACCCGCATCTGAGCTCGACT  >  NC_002947/1606302‑1606590
                                                                                                                                                   |                                                                                                                                             
tttCGAATAGTTTCACGAATCCAGTGAGCATATCCGCCACAAGCCGTTTCGCTTGCGCACGCAAAGGCCCGATGCGCATTGAAGCGGATCGGTGGGTAATTGGCCATGGGCCAGGTCAAGGCAGGATGAAAAACGAAAAAAGGAGCCAAg                                                                                                                                             >  2:171744/1‑150 (MQ=255)
            tCACGAATCCAGTGAGCATATCCGCCACAAGCCGTTTCGCTTGCGCACGCAAAGGCCCGATGCGCATTGAAGCGGATCGGTGGGTAATTGGCCATGGGCCAGGTCAAGGCAGGATGAAAAACGAAAAAAGGAGCCTTGGCTCCtt                                                                                                                                      <  2:340530/145‑1 (MQ=255)
            tCACGAATCCAGTGAGCATATCCGCCACAAGCCGTTTCGCTTGCGCACGCAAAGGCCCGATGCGCATTGAAGCGGATCGGTGGGTAATTGGCCATGGGCCAGGTCAAGGCAGGATGAAAAACGAAAAAAGGAGCCTTGGCTCCtt                                                                                                                                      >  1:340530/1‑145 (MQ=255)
                       gTGAGCATATCCGCCACAAGCCGTTTCGCTTGCGCACGCAAAGGCCCGATGCGCATTGAAGCGGATCGGTGGGTAATTGGCCATGGGCCAGGTCAAGGCAGGATGAAAAACGAAAAAAGGAGTCAAGGCTCCTTTTGACTGCGATGACGt                                                                                                                      >  1:64406/1‑150 (MQ=255)
                              tatCCGCCACAAGCCGTTTCGCTTGCGCACGCAAAGGCCCGATGCGCATTGAAGCGGATCGGTGGGTAATTGGCCATGGGCCAGGTCAAGGCAGGATGAAAAACGAAAAAAGGAGCCAAGGCTCCTTTTGACTGCGATGACGTTGCTGGa                                                                                                               >  1:291450/1‑150 (MQ=255)
                               atCCGCAACAAGCAGTTTCGCTTGCGCCCACAAAGGCAGGACGCGCAGTGAACCGGAGCGGTGGGTAATTGGCCATGGGCCAGGTCAAGGAAGGAAAAAAAAAGAAAAAAGGAGCCAAGGCTCCTTTTGACTGCGATGACGTTGCTGGaa                                                                                                              <  2:55751/150‑1 (MQ=255)
                                tCCGCCACAAGCCGTTTCGCTTGCGCACGCAAAGGCCCGATGCGCATTGAAGCGGATCGGTGGGTAATTGGCCATGGGCCAGGTCAAGGCAGGATGAAAAACGAAAAAAGGAGCCAAGGCTCCTTTTGACTGCGATGACGTTGCTGGAAc                                                                                                             <  1:171744/150‑1 (MQ=255)
                                tCCGCCACAAGCCGTTTCGCTTGCGCACGCAAAGGCCCGATGCGCATTGAAGCGGATCGGTGGGTAATTGGCCATGGGCCAGGTCAAGGCAGGATGAAAAACGAAAAAAGGAGCCAAGGCTCCTTTTGACTGCGATGACGTTGCTGGAAc                                                                                                             <  2:291450/150‑1 (MQ=255)
                                tCCGCCACAAGCCGTTTCGCTTGCGCACGCAAAGGCCCGATGCGCATTGAAGCGGATCGGGGGGTAATTGGCCATGGGCCAGGTCAAGGCAGGATGAAAAACGAAAAAAGAAGCCAAGGCGCCTTTTGACTGCGATGACGTTGCTGGAAc                                                                                                             >  1:360289/1‑150 (MQ=255)
                                                            gcaAAGGCCCGATGCGCATTGAAGCGGATCGGTGGGTAATTGGCCATGGGCCAGGTCAAGGCAGGATGAAAAACGAAAAAAGGAGCCAAGGCTCCTTTTGACTGCGATGACGTTGCTGGAACGCCATGCGGGACTGCGATGTGGAGCggg                                                                                 >  1:189327/1‑150 (MQ=255)
                                                                  gCCCGATGCGCATTGAAGCGGATCGGTGGGTAATTGGCCATGGGCCAGGTCAAGGCAGGATGAAAAACGAAAAAAGGAGCCAAGGCTCCTTTTGACTGCGATGACGTTGCTGGAACGCCATGCGGGACTGCGATGTGGAGCGGGTAGAgg                                                                           <  2:466259/150‑1 (MQ=255)
                                                                     cGATGCGCATTGAAGCGGATCGGTGGGTAATTGGCCATGGGCCAGGTCAAGGCAGGATGAAAAACGAAAAAAGGAGCCAAGGCTCCTTTTGACTGCGATGACGTTGCTGGAACGCCATGCGGGACTGCGATGTGGAGCGGGTAGAGGGaa                                                                        <  2:64406/150‑1 (MQ=255)
                                                                           gcATTGAAGCGGATCGGTGGGTAATTGGCCATGGGCCAGGTCAAGGCAGGATGAAAAACGAAAAAAGGAGCCAAGGCTCCTTTTGACTGCGATGACGTTGCTGGAACGCCATGCGGGACTGCGATGTGGAGCGGGTAGAGGGAATCGAAc                                                                  >  2:219897/1‑150 (MQ=255)
                                                                             aTTGAAGCGGATCGGTGGGTAATTGGCCATGGGCCAGGTCAAGGCAGGATGAAAAACGAAAAAAGGAGCCAAGGCTCCTTTTGACTGCGATGACGTTGCTgg                                                                                                                >  2:283805/1‑102 (MQ=255)
                                                                             aTTGAAGCGGATCGGTGGGTAATTGGCCATGGGCCAGGTCAAGGCAGGATGAAAAACGAAAAAAGGAGCCAAGGCTCCTTTTGACTGCGATGACGTTGCTgg                                                                                                                <  1:283805/102‑1 (MQ=255)
                                                                                 aaGCGGATCGGGGGGTAATTGGCCATGGGCCAGGTCAAGGCAGGATGAAAAACGAAAAAAGGAGCCAAGGCTCCTTTTGACTGCGATGACGTTGCTGGAACGCCATGCGGGACTGCGATGTGGAGCTGGTAGAGGGAATCGAACCCTCaa                                                            <  2:181045/150‑1 (MQ=255)
                                                                                                     ggCCATGGGCCAGGTCAAGGCAGGATGAAAAACGAAAAAAGGAGCCAAGGCTCCTTTTGACTGCGATGACGTTGCTGGAACGCCATGCGGGACTGCGATGTGGAGCGGGTAGAGGGAATCGAACCCTCAACTAAAGCTTGGGAAGCTTTc                                        <  2:189327/150‑1 (MQ=255)
                                                                                                      gCCATGGGCCAGGTCAAGGCAGGATGAAAAACGAAAAAAGGAGCCAAGGCTCCTTTTGACTGCGATGACGTTGCTGGAACGCCATGCGGGACTGCGATGTGGAGCGGGTaga                                                                             >  1:325250/1‑112 (MQ=255)
                                                                                                      gCCATGGGCCAGGTCAAGGCAGGATGAAAAACGAAAAAAGGAGCCAAGGCTCCTTTTGACTGCGATGACGTTGCTGGAACGCCATGCGGGACTGCGATGTGGAGCGGGTaga                                                                             <  2:325250/112‑1 (MQ=255)
                                                                                                             gCCAGGTCAAGGCAGGATGAAAAACGAAAAAAGGAGCCAAGGCTCCTTTTGACTGCGATGACGTTGCTGGAACGCCATGCGGGACTGCGATGTGGAGCGGGTAGAGGGaa                                                                        >  1:434756/1‑110 (MQ=255)
                                                                                                             gCCAGGTCAAGGCAGGATGAAAAACGAAAAAAGGAGCCAAGGCTCCTTTTGACTGCGATGACGTTGCTGGAACGCCATGCGGGACTGCGATGTGGAGCGGGTAGAGGGaa                                                                        <  2:434756/110‑1 (MQ=255)
                                                                                                                     aaGGCAGGATGAAAAACGAAAAAAGGAGCCAAGGCTCCTTTTGACTGCGATGACGTTGCTGGAACGCCATGCGGGACTGCGATGTGGAGCGGGTAGAGGGAATCGAACCCTCAACTAAAGCTTGGGAAGCTTTCGTTTTGCCACTAAACt                        >  1:165753/1‑150 (MQ=255)
                                                                                                                         cAGGATGAAAAACGAAAAAAGGAGCCAAGGCTCCTTTTGACTGCGATGACGTTGCTGGAACGCCATGCGGGACTGCGATGTGGAGCGGGTAGAGGGAATCGAACCCTCAACTAAAGCTTGGGAAGCTTTCGTTTTGCCACTAAACTATAc                    <  2:165753/150‑1 (MQ=255)
                                                                                                                                 aaaaCGAAAAAAGGAGCCAAGGCTCCTTTTGACTGCGATGACGTTGCTGGAACGCCATGCGGGACTGCGATGTGGAGCGGGTAGAGGGAATCGAACCCTCAACTAAAGCTTGGGAAGCTTTCGTTTTGCCACTAAACTATACCCGCATCt            <  1:219897/150‑1 (MQ=255)
                                                                                                                                      gAAAAAAGGAGCCAAGGCTCCTTTTGACTGCGATGACGTTGCTGGAACGCCATGCGGGACTGCGATGTGGAGCGGGTAGAGGGAATCGAACCCTCAACTAAAGCTTGGGAAGCTTTCGTTTTGCCACTAAACTATACCCGCATCTGAGCt       >  2:73950/1‑150 (MQ=255)
                                                                                                                                      gAAAAAAGGAGCCAAGGCTCCTTTTGACTGCGATGACGTTGCTGGAACGCCATGCGGGACTGCGATGTGGAGCGGGTAGAGGGAATCGAACCCTCAACTAAAGCTTGGGAAGCTTTCGTTTTGCCACTAAACTATACCCGCATCTGAGCt       <  1:73950/150‑1 (MQ=255)
                                                                                                                                         aaaaGGAGCCTTGGCTCCTTTTGACTGCGATGACGTTGCTGGAACGCCATGCGGGACTGCGATGTGGAGCGGGTAGAGGGAATCGAACCCTCAACTAAAGCTTGGGAAGCTTTCGTTTTGCCACTAAACTATACCCGCATCt            >  2:479945/1‑142 (MQ=255)
                                                                                                                                         aaaaGGAGCCTTGGCTCCTTTTGACTGCGATGACGTTGCTGGAACGCCATGCGGGACTGCGATGTGGAGCGGGTAGAGGGAATCGAACCCTCAACTAAAGCTTGGGAAGCTTTCGTTTTGCCACTAAACTATACCCGCATCt            <  1:479945/142‑1 (MQ=255)
                                                                                                                                         aaaaGGAGCCAAGGCTCCTTTTGACTGCGATGACGTTGCTGGAACGCCATGCGGGACTGCGATGTGGAGCGGGTAGAGGGAATCGAACCCTCAACTAAAGCTTGGGAAGCTTTCGTTTTGCCACTAAACTATACCCGCATCTGAGc        <  2:278071/146‑1 (MQ=255)
                                                                                                                                         aaaaGGAGCCAAGGCTCCTTTTGACTGCGATGACGTTGCTGGAACGCCATGCGGGACTGCGATGTGGAGCGGGTAGAGGGAATCGAACCCTCAACTAAAGCTTGGGAAGCTTTCGTTTTGCCACTAAACTATACCCGCATCTGAGc        >  1:278071/1‑146 (MQ=255)
                                                                                                                                           aaGGAGCCTTGGCTCCTTTTGACTGCGATGACGTTGCTGGAACGCCATGCGGGACTGCGATGTGGAGCGGGTAGAGGGAATCGAACCCTCAACTAAAGCTTGGGAAGCTTTCGTTTTGCCACTAAACTATACCCGCATCTGAGCTCGACt  >  2:406469/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                             
TTTCGAATAGTTTCACGAATCCAGTGAGCATATCCGCCACAAGCCGTTTCGCTTGCGCACGCAAAGGCCCGATGCGCATTGAAGCGGATCGGTGGGTAATTGGCCATGGGCCAGGTCAAGGCAGGATGAAAAACGAAAAAAGGAGCCAAGGCTCCTTTTGACTGCGATGACGTTGCTGGAACGCCATGCGGGACTGCGATGTGGAGCGGGTAGAGGGAATCGAACCCTCAACTAAAGCTTGGGAAGCTTTCGTTTTGCCACTAAACTATACCCGCATCTGAGCTCGACT  >  NC_002947/1606302‑1606590

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑