| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 1,852,445 | C→T | 18.5% | R497W (CGG→TGG) | relA → | ATP:GTP 3'‑pyrophosphotransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,852,445 | 0 | C | T | 18.5% | 49.5 / 7.3 | 27 | R497W (CGG→TGG) | relA | ATP:GTP 3'‑pyrophosphotransferase |
| Reads supporting (aligned to +/- strand): ref base C (11/11); new base T (2/3); total (13/14) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.64e-01 | |||||||||||
GCGGCGCGAAGATCAACGGCCGTATCGTGCCGCTGAACTACAGCCTGCAGACTGGCGAGCAGGTGGAGATCATCACCAGCAAGCACGGCAACCCCAGCCGTGACTGGTTGAACTCCAACCTGGGCTACGTCACCACCTCGCGGGCGCGGGCCAAGATCGTCCACTGGTTCAAATTGCAGGCCCGCGACCAGAACGTTGCTGCCGGCAAGACCTTGCTTGAGCGCGAGCTCAGTCGTCTGGGCCTGCCGCAGGTTGATTTCGAGCGCCTGGCCGAGAAGACCAACGT > NC_002947/1852305‑1852590 | gcggcgCGAAGATCAACGGCCGTATCGTGCCGCTGAACTACAGCCTGCAGACTGGCGAGCAGGTGGAGATCATCACCAGCAAGCACGGCAACCCCAGCCGTGACTGGTTGAACTCCAACCTGGGCTACGTCACCACCTcgcgggcgcggg > 1:293669/1‑150 (MQ=255) aTCGTGCCGCTGAACTACAGCCTGCAGACTGGCGAGCAGGTGGAGATCATCACCAGCAAGCACGGCAACCCCAGCCGTGACTGGTTGAACTCCAACCTGGGCTACGTCACCACCTCGCGGGCGCGGGCCAAGATCGTCCACTGGTTCaaa > 2:507724/1‑150 (MQ=255) gTGCCGCTGAACTACAGCCTGCAGACTGGCGAGCAGGTGGAGATCATCACCAGCAAGCACAGCAACCCCAGCCGTGACTGATTGAACTCCAACCTGGGCTACGTCACCACCTGGCGGGCGCGGGCCAAGATCGTCCACTGGTTCAAATTg > 2:185547/1‑150 (MQ=255) cTACAGCCTGCAGACTGGCGAGCAGGTGGAGATCATCACCAGCAAGCACGGCAACCCCAGCCGTGACTGGTTGAACTCCAACCTGGGCTACGTCACCACCTCGCGGGCGCGGGCCAAGATCGTCCACTGGTTCAAATTGCAGGCCCGCGa < 2:129567/150‑1 (MQ=255) gCGAGCAGGTGGAGATCATCACCAGCAAGCACGGCAACCCCAGCCGTGACTGGTTGAACTCCAACCTGGGCTACGTCACCACCTCGCGGGCGCGGGCCAAGATCGTCCACTGGTTCAAATTGCAGGCCCGCGACCAGAACGTTGCTGCCg > 1:311103/1‑150 (MQ=255) ggAGATCATCACCAGCAAGCACGGCAACCCCAGCCGTGACTGGTTGAACTCCAACCTGGGCTACGTCACCACCTCGCGGGCGCGGGCCAAGATCGTCCACTGGTTCAAATTGCAGGCCCGCGACCAGAACGTTGCTGCCGGCAAGACctt > 1:384806/1‑150 (MQ=255) gagaTCATCACCAGCAAGCACGGCAACCCCAGCCGTGACTGGTTGAACTCCAACCTGGGCTACGTCACCACCTCGCGGGCGCGGGCCAAGATCGTCCACTGGTTCAAATTGCAGGCCCGCGACCAGAACGTTGCTGCCGGCAAGACcttg > 1:10525/1‑150 (MQ=255) caCCAGCAAGCACGGCAACCCCAGCCGTGACTGGTTGAACTCCAACCTGGGCTACGTCACCACCTCGTGGGCGCGGGCCAAGATCGTCCACTGGTTCAAATTGCAGGCCCGCGACCAGAACGTTGCTGCCGGCAAGACCTTGCTTGAgcg > 1:372848/1‑150 (MQ=255) cAGCAAGCACGGCAACCCCAGCCGTGACTGGTTGAACTCCAACCTGGGCTACGTCACCACCTCGCGGGCGCGGGCCAAGATCGTCCACTGGTTCAAATTGCAGGCCCGCGACCAGAACGTTGCTGCCGGCAAGACCTTGCTTGAGCGCGa > 1:145935/1‑150 (MQ=255) gCAACCCCAGCCGTGACTGGTTGAACTCCAACCTGGGCTACGTCACCACCTCGCGGGCGCGGGCCAAGATCGTCCACTGGTTCAAATTGc > 2:98986/1‑90 (MQ=255) gCAACCCCAGCCGTGACTGGTTGAACTCCAACCTGGGCTACGTCACCACCTCGCGGGCGCGGGCCAAGATCGTCCACTGGTTCAAATTGc < 1:98986/90‑1 (MQ=255) cAACCCCAGCCGTGACTGGTTGAACTCCAACCTGGGCTACGTCACCACCTCGCGGGCGCGGGCCAAGATCGTCCACTGGTTCAAATTGCAGGcc < 1:465668/94‑1 (MQ=255) cAACCCCAGCCGTGACTGGTTGAACTCCAACCTGGGCTACGTCACCACCTCGCGGGCGCGGGCCAAGATCGTCCACTGGTTCAAATTGCAGGcc > 2:465668/1‑94 (MQ=255) aaCCCCAGCCGTGACTGGTTGAACTCCAACCTGGGCTACGTCACCACCTCGCGGGCGCGGGCCAAGATCGTCCACTGGTTCAAATTGCAGGCCCGCGACCAGAACGTTGCTGCCGGCAAGACCTTGCTTGAGCGCGAGCTCAGTCGTCTg < 2:326912/150‑1 (MQ=255) tGACTGGTTGAACTCCAACCTGGGCTACGTCACCACCTCGCGGGCGCGGGCCAAGATCGTCCACTGGTTCAAATTGCAGGCCCGCGACCAGAACGTTGCTGCCGGCAAGACCTTGCTTGAGCGCGAGCTCAGTCGTCTGGGCCTGCCGCa < 2:293669/150‑1 (MQ=255) gACTGGTTGAACTCCAACCTGGGCTACGTCACCACCTCGTGGGCGCGGGCCAAGATCGTCCACTGGTTCAAATTGCAGGCCCGCGACCAGAACGTTGCTGCCGGCAAGACCTTGCTTGAGCGCGAGCTCAGTCGTCTGGACCTGCCGCAg < 2:372848/150‑1 (MQ=255) cTGGTTGAACTCCAACCTGGGCTACGTCACCACCTCGTGGGCGCGGGCCAAGATCGTCCACTGGTTCAAATTGCAGGCCCGCGACCAGAACGTTGCTGCCGGCAAGACCTTGCTTGAGCGCGAGCTCAGTCGTCTGGGCCTGCCGCAGGt < 2:160066/150‑1 (MQ=255) tGGTTGAACTCCAACCTGGGCTACGTCACCACCTCGCGGGCGCGGGCCAAGATCGTCCACTGGTTCAAATTGCAAGATTGGCAAGCTAACGTTGCTGCCGGCAAGACCTTGCTTGAACGCGAGCTCAGTCGTCTGGGCCTGCCGCAGGtt > 1:342668/1‑150 (MQ=255) ggTTGAACTCCAACCTGGGCTACGTCACCACCTCGCGGGCGCGGGCCAAGATCGTCCACTGGTTCAAATTGCAGGCCCGCGACCAGAACGTTGCTGCCGGCAAGACCTTGCTTGAGCGCGAGCTCAGTCGTCTGGGCCTGCCGCAGGTTg < 2:10525/150‑1 (MQ=255) gTTGAACTCCAACCTGGGCTACGTCACCACCTCGTGGGCGCGGGCCAAGATCGTCCACTg < 1:402536/60‑1 (MQ=255) gTTGAACTCCAACCTGGGCTACGTCACCACCTCGTGGGCGCGGGCCAAGATCGTCCACTg > 2:402536/1‑60 (MQ=255) ttGAACTCCAACCTGGGCTACGTCACCACCTCGCGGGCGCGGGCCAAGATCGTCCACTGGTTCAAATTGCAGGCCCGCGACCAGAACGTTGCTGCCGGCAAGACCTTGCTTGAGCGCGAGCTCAGTCGTCTGGGCCTGCCGCAGGTTGAt > 1:119376/1‑150 (MQ=255) ttGAACTCCAACCTGGGCTACGTCACCACCTCGCGGGCGCGGGCCAAGATCGTCCACTGGTTCAAATTGCAGGCCCGCGACAAGAACGTTGCTGCAGGCAAGACCTTGCTTGAGCGCGAGCTCAGTCGTCTGGGCCTGCCGCAGGTTGAt < 1:55538/150‑1 (MQ=255) cTCCAACCTGGGCTACGTCACCACCTCGCGGGCGCGGGCCAAGATCGTCCACTGGTTCAAATTGCAGGCCCGCGACCAGAACGTTGCTGCCGGCAAGACCTTGCTTGAGCGCGAGCTCAGTCGTCTGGGCCTGCCGCAGGTTGATTTCGa < 2:311103/150‑1 (MQ=255) cTCCAACCTGGGCTACGTCACCACCTCGCGGGCGCGGGCCAAGATCGTCCACTGGTTCAAATTGCAGGCCCGCGACCAGAACGTTGCTGCCGGCAAGACCTTGCTTGAGCGCGAGCTCAGTCGTCTGGGCCTGCCGCAGGTTGATTTCGa < 1:507724/150‑1 (MQ=255) cTCCAACCTGGGCTACGTCACCACCTCGCGGGCGCGGGCCAAGATCGTCCACTGGTTCAAATTGCAGGCCCGCGACCAGAACGTTGCTGCCGGCAAGACCTTGCTTGAGCGCGAGCTCAGTCGTCTGGGCCTGCCGCAGGTTGATTTCGa < 2:86082/150‑1 (MQ=255) aaCCTGGGCTACGTCACCACCTCGCGGGCGCGGGCCAAGATCGTCCACTGGTTCAAATTGCAGGCCCGCGACCAGAACGTTGCTGCCGGCAAGACCTTGCTTGAGCGCGAGCTCAGTCGTCTGGGCCTGCCGCAGGTTGATTTCGAgcgc < 2:384806/150‑1 (MQ=255) cTCGCGGGCGCGGGCCAAGATCGTCCACTGGTTCAAATTGCAGGCCCGCGACCAGAACGTTGCTGCCGGCAAGACCTTGCTTGAGCGCGAGCTCAGTCGTCTGGGCCTGCCGCAGGTTGATTTCGAGCGCCTGGCCGAGAAGACCAACGt > 2:349204/1‑150 (MQ=255) | GCGGCGCGAAGATCAACGGCCGTATCGTGCCGCTGAACTACAGCCTGCAGACTGGCGAGCAGGTGGAGATCATCACCAGCAAGCACGGCAACCCCAGCCGTGACTGGTTGAACTCCAACCTGGGCTACGTCACCACCTCGCGGGCGCGGGCCAAGATCGTCCACTGGTTCAAATTGCAGGCCCGCGACCAGAACGTTGCTGCCGGCAAGACCTTGCTTGAGCGCGAGCTCAGTCGTCTGGGCCTGCCGCAGGTTGATTTCGAGCGCCTGGCCGAGAAGACCAACGT > NC_002947/1852305‑1852590 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |