| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 1,944,672 | A→G | 26.7% | intergenic (+130/+132) | actP‑I → / ← PP_1744 | acetate permease/hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,944,672 | 0 | A | G | 26.7% | 19.0 / 5.1 | 15 | intergenic (+130/+132) | actP‑I/PP_1744 | acetate permease/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base A (5/6); new base G (2/2); total (7/8) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.24e-01 | |||||||||||
TCCCCTGCATTGAAAACGCCCCGCTTGTCGGGGCGTTTTTTATTTCATGCAGTCGGCGATGGCCTCCAGACAGGTTGGTGCTCTGCCCATGCTCCCCTCATGTCGTGAGCGCCTTCATGGAGGACGGCACATTTGGGGGCTGGTCAATGAAGCCATACTGTTTAATCACCTTCGCTATCCAGGATGGCAACCGGGTATTGCGACCCGCTTGCCATTCACCTCACGTGGCTTTTTGAAGTGGCTATCCGCAGCGCGTCATATCATCCC > NC_002947/1944549‑1944815 | tCCCCTGCATTGAAAACGCCCCGCTTGTCGGGGCGTTTTTTATTTCATGCAGTCGGCGATGGCCTCCAGACAGGTTGGTGCTCTGCCCATGCTCCCCTCATGTCGTGAGCGCCTTCATGGAGGGCGGCACATTTggg > 1:383208/1‑137 (MQ=255)tCCCCTGCATTGAAAACGCCCCGCTTGTCGGGGCGTTTTTTATTTCATGCAGTCGGCGATGGCCTCCAGACAGGTTGGTGCTCTGCCCATGCTCCCCTCATGTCGTGAGCGCCTTCATGGAGGGCGGCACATTTggg < 2:383208/137‑1 (MQ=255) cTGCATTGAAAACGCCCCGCTTGTCGGGGCGTTTTTTATTTCATGCAGTCGGCGATGGCCTCCAGACAGGTTTGTGCTCTGCCCATGCTCCCCTCATGTCGTGAGCGCCTTCATGGAGGACGGCACATTTGGGGGCTGGTCAATGAAGcc < 1:366698/150‑1 (MQ=255) aaaCGCCCCGCTTGTCGGGGCGTTTTTTATTTCATGCAGTCGGCGATGGCCTCCAGACAGGTTGGTGCTCTGCCCATGCTCCCCTCATGTCGTGAGCGCCTTCATGGAGGACGGCACATTTg > 1:472234/1‑122 (MQ=255) aaaCGCCCCGCTTGTCGGGGCGTTTTTTATTTCATGCAGTCGGCGATGGCCTCCAGACAGGTTGGTGCTCTGCCCATGCTCCCCTCATGTCGTGAGCGCCTTCATGGAGGACGGCACATTTg < 2:472234/122‑1 (MQ=255) gCTCTGCCCATGCTCCCCTCATGTCGTGAGCGCCTTCATGGAGGACGGCACATTTGGGGGCTGGTCAATGAAGCCATACTGTTTAATCACCTTCGCTATCCAGGATGGCAACCGGGTATTGCGACCCGCTTGCCATTCACCTCACGTGGc > 2:232752/1‑150 (MQ=255) cTCCCCTCATGTCGTGAGCGCCTTCATGGAGGACGGCACATTTGGGGGCTGGTCAATGAAGCCATACTGTTTAATCACCTTCGCTATCCAGGATGGCAACCGGGTATTGCGACCCGCTTGCCATTCACCTCACGTGGCTTTTTGAAGTgg > 2:311708/1‑150 (MQ=255) cTCCCCTCATGTCGTGAGCGCCTTCAGGGAGGACGGCACATTTGGGGGCTGGTCAATGAAGCCATACTGTTTAATCACCTTCGCTATCCAGGATGGCAACCGGGTATTGCGACCCGCTTGCCATTCACCTCACGTGGCTTTTTGAAGTgg < 1:232752/150‑1 (MQ=255) tCCCCTCATGTCGTGAGCGCCTTCATGGAGGACGGCACATTTGGGGGCTGGTCAATGAAGCCATACTGTTTAATCACCTTCGCTATCCAGGATGGCAACCGGGTATTGCGACCCGCTTGCCATTCACCTCACGTGGCTTTTTGAAGTgg > 1:175923/1‑149 (MQ=255) tCCCCTCATGTCGTGAGCGCCTTCATGGAGGACGGCACATTTGGGGGCTGGTCAATGAAGCCATACTGTTTAATCACCTTCGCTATCCAGGATGGCAACCGGGTATTGCGACCCGCTTGCCATTCACCTCACGTGGCTTTTTGAAGTgg < 2:175923/149‑1 (MQ=255) ccTCATGTCGTGAGCGCCTTCATGGAGGGCGGCACATTTGGGGGCTGGTCAATGAAGCCATACTGTTTAATCACCTTCGCTATCCAGGATGGCAACCGGGTATTGCGACCCGCTTGc > 1:318865/1‑117 (MQ=255) ccTCATGTCGTGAGCGCCTTCATGGAGGGCGGCACATTTGGGGGCTGGTCAATGAAGCCATACTGTTTAATCACCTTCGCTATCCAGGATGGCAACCGGGTATTGCGACCCGCTTGc < 2:318865/117‑1 (MQ=255) cTTCATGGAGGACGGCACATTTGGGGGCTGGTCAATGAAGCCATACTGTTTAATCACCTTCGCTATCCAGGa < 1:68716/72‑1 (MQ=255) cTTCATGGAGGACGGCACATTTGGGGGCTGGTCAATGAAGCCATACTGTTTAATCACCTTCGCTATCCAGGa > 2:68716/1‑72 (MQ=255) tGGAGGACGGCACATTTGGGGGCTGGTCAATGAAGCCATACTGTTTAATCACCTTCGCTATCCAGGATGGCAACCGGGTATTGCGACCCGCTTGCCATTCACCTCACGTGGCTTTTTGAAGTGGCTATCCGCAGCGCGTCATATCATccc < 1:311708/150‑1 (MQ=255) | TCCCCTGCATTGAAAACGCCCCGCTTGTCGGGGCGTTTTTTATTTCATGCAGTCGGCGATGGCCTCCAGACAGGTTGGTGCTCTGCCCATGCTCCCCTCATGTCGTGAGCGCCTTCATGGAGGACGGCACATTTGGGGGCTGGTCAATGAAGCCATACTGTTTAATCACCTTCGCTATCCAGGATGGCAACCGGGTATTGCGACCCGCTTGCCATTCACCTCACGTGGCTTTTTGAAGTGGCTATCCGCAGCGCGTCATATCATCCC > NC_002947/1944549‑1944815 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |