| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 1,978,751 | C→G | 22.2% | F45L (TTC→TTG) | cmk → | cytidylate kinase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,978,751 | 0 | C | G | 22.2% | 53.0 / 12.9 | 27 | F45L (TTC→TTG) | cmk | cytidylate kinase |
| Reads supporting (aligned to +/- strand): ref base C (11/10); new base G (3/3); total (14/13) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.72e-01 | |||||||||||
GGCAAGTCGTGAATTCAATGGCACCGGTTATCACCATCGACGGACCAAGCGGCTCGGGCAAGGGCACGGTCGCCGGGCTGATTGCCCGCGAGCTGGGCTGGAAGCTGCTGGACTCCGGCGCGCTGTATCGCTTGCTGGCATTCAATGCCAGCAACCACGGCGTCGACCTGACCAACGAAGAGCTGCTGACCAAGCTTGCCGCCCATCTGGATGTGCAGTTCATCGCTGCCGAGCCCGGTAAGCTACAACAGATCATCCTTGAGGGTGAGGACGTCAGCAACGTCATCCGT > NC_002947/1978609‑1978898 | ggCAAGTCGTTAATTCAATGGCACCGGTTATCACCAGCGACGAACCAAGCGGCTCGGGCAAGGGCACGGTCGCCGGGCTGATTGCCCGCGAGCTGGGCTGGAAGCTGCTGGACTCCGGCGCGCTGTATCGCTTGCTGGCATTCAATGCCa < 2:194064/150‑1 (MQ=255) aTTCAATGGCACCGGTTATCACCATCGACGGACCAAGCGGCTCGGGCAAGGGCACGGTCGCCGGGCTGATTGCCCGCGAGCTGGGCTGGAAGCTGCTGGACTCCGGCGCGCTGTATCGCTTGCTGGCATTCAATGCCAGCAACCACGGCg > 1:333026/1‑150 (MQ=255) ttCAATGGCACCGGTTATCACCATCGACGGACCAAGCGGCTCGGGCAAGGGCACGGTCGCCGGGCTGATTGCCCGCGAGCTGGGCTGGAAGCTGCTGGACTCCGGCGCGCTGTATCGCTTGCTGGCATTGAATGCCAGCaa > 2:431621/1‑141 (MQ=255) ttCAATGGCACCGGTTATCACCATCGACGGACCAAGCGGCTCGGGCAAGGGCACGGTCGCCGGGCTGATTGCCCGCGAGCTGGGCTGGAAGCTGCTGGACTCCGGCGCGCTGTATCGCTTGCTGGCATTGAATGCCAGCaa < 1:431621/141‑1 (MQ=255) tGGCACCGGTTATCACCATCGACGGACCAAGCGGCTCGGGCAAGGGCACGGTCGCCGGGCTGATTGCCCGCGAGCTGGGCTGGAAGCTGCTGGACTCCGGCGCGCTGTATCGCTTGCTGGCATTCAATGCCAGCAAc > 2:495583/1‑137 (MQ=255) tGGCACCGGTTATCACCATCGACGGACCAAGCGGCTCGGGCAAGGGCACGGTCGCCGGGCTGATTGCCCGCGAGCTGGGCTGGAAGCTGCTGGACTCCGGCGCGCTGTATCGCTTGCTGGCATTCAATGCCAGCAAc < 1:495583/137‑1 (MQ=255) gCACCGGTTATCACCATCGACGGACCAAGCGGCTCGGGCAAGGGCACGGTCGCCGGGCTGATTGCCCGCGAGCTGGGCTGGAAGCTGCTGGACTCCGGCGCGCTGTATCGCTTGCTGGCATTCAATGCCAGCAACCACGGCGTCGACCTg > 1:166389/1‑150 (MQ=255) aCCGGTTATCACCATCGACGGACCAAGCGGCTCGGGCAAGGGCACGGTCGCCGGGCTGATTGCCCGCGAGCTGGGCTGGAAGCTGCTGGACTCCGGCGCGCTGTATCGCTTGCTGGCATTCa < 2:393594/122‑1 (MQ=255) aCCGGTTATCACCATCGACGGACCAAGCGGCTCGGGCAAGGGCACGGTCGCCGGGCTGATTGCCCGCGAGCTGGGCTGGAAGCTGCTGGACTCCGGCGCGCTGTATCGCTTGCTGGCATTCa > 1:393594/1‑122 (MQ=255) aTCGACGGACCAAGCGGCTCGGGCAAGGGCACGGTCGCCGGGCTGATTGCCCGCGAGCTGGGCTGGAAGCTGCTGGACTCCGGCGCGCTGTATCGCTTGCTGGCATTGAATGCCAGCaagc < 1:504511/121‑3 (MQ=255) aTCGACGGACCAAGCGGCTCGGGCAAGGGCACGGTCGCCGGGCTGATTGCCCGCGAGCTGGGCTGGAAGCTGCTGGACTCCGGCGCGCTGTATCGCTTGCTGGCATTGAATGCCAGCaagc > 2:504511/1‑119 (MQ=255) aTCGACGGACCAAGCGGCTCGGGCAAGGGCACGGTCGCCGGGCTGATTGCCCGCGAGCTGGGCTGGAAGCTGCTGGACTCCGGCGCGCTGTATCGCTTGCTGGCATTCAATGCCAGCAACCACGGCGTCGACCTGACCAACGAAGAgctg > 1:172190/1‑150 (MQ=255) tCGACGGACCAAGCGGCTCGGGCAAGGGCACGGTCGCCGGGCTGATTGCCCGCGAGCTGGGCTCGAAGCCGCTGGACTCCGGCGCGCTGTATCGCTTGCTGGCATTCAATGCCAGCAACCACGGCGTCGACCTGACCAACGAAGAgctgc < 1:61607/150‑1 (MQ=255) cGACGGACCAAGCGGCTCGGGCAAGGGCACGGTCGCCGGGCTGATTGCCCGCGAGCTGGGCTGGAAGCTGCTGGACTCCGGCGCGCTGTATCGCTTGCTGGCATTCAATGCCAGCAACCACGGCGTCGACCTGACCAACGAAGAgctgct < 2:333026/150‑1 (MQ=255) aCCAAGCGGCTCGGGCAAGGGCACGGTCGCCGGGCTGATTGCCCGCGAGCTGGGCTGGAAGCTGCTGGACTCCGGCGCGCTGTATCGCTTGCTGGCATTGAATGCCAGCa < 1:430401/110‑1 (MQ=255) aCCAAGCGGCTCGGGCAAGGGCACGGTCGCCGGGCTGATTGCCCGCGAGCTGGGCTGGAAGCTGCTGGACTCCGGCGCGCTGTATCGCTTGCTGGCATTGAATGCCAGCa > 2:430401/1‑110 (MQ=255) cTCGGGCAAGGGCACGGTCGCCGGGCTGATTGCCCGCGAGCTGGGCTGGAAGCTGCTGGACTCCGGCGCGCTGTATCGCTTGCTGGCATTCAATGCCAGCAACCACGGCGTCGACCTGACCAACGAAGAGCTGCTGACCAAGCTTgccgc < 2:166389/150‑1 (MQ=255) cGGGCTGATTGCCCGCGAGCTGGGCTGGAAGCTGCTGGACTCCGGCGCGCTGTATCGCTTGCTGGCATTCAATGCCAGCAACCACGGCGTCGACCTGACCAACGAAGAGCTGCTGACCAAGCTTGCCGCCCATCTGGATGTGCAGTTCAt > 1:119093/1‑150 (MQ=255) cccGCGAGCTGGGCTGGAAGCTGCTGGACTCCGGCGCGCTGTATCGCTTGCTGGCATTCAATGCCAGCAACCACGGCGTCGACCTGACCAACGAAGAGCTGCTGACCAAGCTTGCCGCCCATCTGGATGTGCAGTTCATCGCTGCCGAGc < 1:475343/150‑1 (MQ=255) aGCTGCTGGACTCCGGCGCGCTGTATCGCTTGCTGGCATTCAATGCCAGCAACCACGGCGTCGACCTGACCAACGAAGAGCTGCTGACCAAGCTTGCCGCCCATCTGGATGTGCAGTTCATCGCTGCCGAGCCCGGTa < 2:373106/138‑1 (MQ=255) aGCTGCTGGACTCCGGCGCGCTGTATCGCTTGCTGGCATTCAATGCCAGCAACCACGGCGTCGACCTGACCAACGAAGAGCTGCTGACCAAGCTTGCCGCCCATCTGGATGTGCAGTTCATCGCTGCCGAGCCCGGTa > 1:373106/1‑138 (MQ=255) gctgGACTCCGGCGCGCTGTATCGCTTGCTGGCATTCAATGCCAGCAACCACGGCGTAGACCTGACCAACGAAGAGCTGCTGACCAAGCTTGCCGCCCATCTGGATGTGCa < 2:379779/111‑1 (MQ=255) gctgGACTCCGGCGCGCTGTATCGCTTGCTGGCATTCAATGCCAGCAACCACGGCGTAGACCTGACCAACGAAGAGCTGCTGACCAAGCTTGCCGCCCATCTGGATGTGCa > 1:379779/1‑111 (MQ=255) ggACTCCGGCGCGCTGTATCGCTTGCTGGCATTCAATGCCAGCAACCACGGCGTCGACCTGACCAACGAAGAGCTGCTGACCAAGCTTGCCGCCCATCTGGATGTGCAGTTCATCGCTGCCGAGCCCGGTAAGCTacaa < 1:413531/139‑1 (MQ=255) ggACTCCGGCGCGCTGTATCGCTTGCTGGCATTCAATGCCAGCAACCACGGCGTCGACCTGACCAACGAAGAGCTGCTGACCAAGCTTGCCGCCCATCTGGATGTGCAGTTCATCGCTGCCGAGCCCGGTAAGCTacaa > 2:413531/1‑139 (MQ=255) gcgcgcTGTATCGCTTGCTGGCATTCAATGCCAGCAACCACGGCGTCGACCTGACCAACGAAGAGCTGCTGACCAAGCTTGCCGCCCATCTGGATGTGCAGTTCATCGCTGCCGAGCCCGGTAAGCTACAACAGATCATCCTTGAGGGTg > 1:212722/1‑150 (MQ=255) ttCAATGCCAGCAACCACGGCGTCGACCTGACCAACGAAGAGCTGCTGACCAAGCTTGCCGCCCATCTGGATGTGCAGTTCATCGCTGCCGAGCCCGGTAAGCTACAACAGATCATCCTTGAGGGTGAGGACGTCAGCAACGTCATCCGt > 2:78366/1‑150 (MQ=255) | GGCAAGTCGTGAATTCAATGGCACCGGTTATCACCATCGACGGACCAAGCGGCTCGGGCAAGGGCACGGTCGCCGGGCTGATTGCCCGCGAGCTGGGCTGGAAGCTGCTGGACTCCGGCGCGCTGTATCGCTTGCTGGCATTCAATGCCAGCAACCACGGCGTCGACCTGACCAACGAAGAGCTGCTGACCAAGCTTGCCGCCCATCTGGATGTGCAGTTCATCGCTGCCGAGCCCGGTAAGCTACAACAGATCATCCTTGAGGGTGAGGACGTCAGCAACGTCATCCGT > NC_002947/1978609‑1978898 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |