| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 2,144,374 | G→T | 21.4% | G227V (GGC→GTC) | lpxK → | tetraacyldisaccharide 4'‑kinase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 2,144,374 | 0 | G | T | 21.4% | 52.8 / 7.8 | 28 | G227V (GGC→GTC) | lpxK | tetraacyldisaccharide 4'‑kinase |
| Reads supporting (aligned to +/- strand): ref base G (9/13); new base T (3/3); total (12/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.39e-01 | |||||||||||
GGGCCGTTGCGTGAGCCGGCCGACCGCTTGCGTGAGGCTGACGCGGTGCTGTTCAACGGCGCCAGCGAAGACCGCACCGATGGTTTCGGTTTCTGCCTGCAGCCGTCGGCCCTGGTCAACGTGCGCACTGGCGAGCGC‑CGTGCGCTCGACCATTTCCCCGCCGGCCAGCGCCTGCATGCGGTGGCCGGTATCGGCAACCCGCAACGTTTCTTCAACACCCTGCTGGGGCTAAACTGGCAGCCGGTGCCGCATCCCTTCGCCGACCATGCGCAGTTCA > NC_002947/2144244‑2144520 | gggCCGTTGCGTGAGCCGGCCGACCGCTTGCGTGAGGCTGACGCGGTGCTGTTCAACGGCGCCAGCGAAGACCGCACCGATGGTTTCGGTTTCTGCCTGCAGCCGTCGGCCCTGGTCAACGTGCGCACTGGCGAGCGC‑CGTGCGCTCGAc < 2:16659/150‑1 (MQ=255) gCGTGAGGCTGACGCGGTGCTGTTCAACGGCGCCAGCGAAGACCGCACCGATGGTTTCGGTTTCTGCCTGCAGCCGTCGGCCCTGGTCAACGTGCGCACTGGCGAGCGC‑CGTGCGCTCGACCATTTCCCCGCCGGCCAGCGCCTGCATGc < 2:150943/150‑1 (MQ=255) gTGAGGCTGACGCGGTGCTGTTCAACGGCGCCAGCGAAGACCGCACCGATGGTTTCGGTTTCTGCCTGCAGCCGTCGGCCCTGGTCAACGTGCGCACTGGCGAGCGC‑CGTGCGCTCGACCATTTCCCCGCCGGCCAGCGCCTGCATGCgg < 1:130553/150‑1 (MQ=255) tGAGGCTGACGCGGTGCCGTTCAACGGCGCCAGCGAAGACCGCACCGATGGTTTCGGTTTCTGCCTCCAGCCGTCGGCCCTGGTCAACGTGCGCACTGGCGATCGC‑CGTACGCTCGACCATTTCCCCGCCGGCCAGTCCCTGCATGCgtg > 2:130553/1‑148 (MQ=255) gcgGTGCTGTTCAACGGCGCCAGCGAAGACCGCACCGATGGTTTCGGTTTCTGCCTGCAGCCGTCGGCCCTGGTCAACGTGCGCACTGGCGAGTGC‑CGTGCGCTCGACCATTTCCCCGCCGGCCAGCGCCTGCATGCGGTGGCCGGTATc > 2:41539/1‑150 (MQ=255) gtgTGCTGTTCAACGGCGCCAGCGAAGACCGCACCGATGGTTTCGGTTTCTGCCTGCAGCCGTCGGCCCTGGTCAACGTGCGCACTGGCGAGCGC‑CGTGCGCTCGACCATTTCCCCGCCGGCCAGCGCCTGCATGCGGTGGCCGGTATCg < 1:381893/148‑1 (MQ=255) gTTCAACGGCGCCAGCGAAGACCGCACCGATGGTTTCGGTTTCTGCCTGCAGCCGTCGGCCCTGGTCAACGTGCGCACTGGCGAGCGC‑CGTGCGCTCGACCATTTcccc > 1:141400/1‑109 (MQ=255) gTTCAACGGCGCCAGCGAAGACCGCACCGATGGTTTCGGTTTCTGCCTGCAGCCGTCGGCCCTGGTCAACGTGCGCACTGGCGAGCGC‑CGTGCGCTCGACCATTTcccc < 2:141400/109‑1 (MQ=255) gTTCAACGGCGCCAGCGAAGACCGCACCGATGGTTTCGGTTTCTGCCTGCAGCCGTCGGCCCTGGTCAACGTGCGCACTGGCGAGCGC‑CGTGCGCTCGACCATTTCCCCGCCGGCCAGCGCCTGCATGCGGTGGCCGGTATCGGCAAccc > 2:398033/1‑150 (MQ=255) ttCAACGGCGCCAGCGAAGACCGCACCGATGGTTTCGGTTTCTGCCTGCAGCCGTCGGCCCTGGTCAACGTGCGCACTGGCGAGCGC‑CGTGCGCTCGACCATTTCCCCACCGGCCACCGCATGCAGGCGCTGGCCggcgg > 1:239310/1‑137 (MQ=255) ttCAACGGCGCCAGCGAAGACCGCACCGATGGTTTCGGTTTCTGCCTGCAGCCGTCGGCCCTGGTCAACGTGCGCACTGGCGAGCGC‑CGTGCGCTCGACCATTTCCCCACCGGCCACCGCATGCAGGCGCTGGCCggcgg < 2:239310/140‑4 (MQ=255) aCGGCGCCAGCGAAGACCGCACCGATGGTTTCGGTTTCTGCCTGCAGCCGTCGGCCCTGGTCAACGTGCGCACTGGCGAgcgcacg < 2:89279/86‑4 (MQ=255) aCGGCGCCAGCGAAGACCGCACCGATGGTTTCGGTTTCTGCCTGCAGCCGTCGGCCCTGGTCAACGTGCGCACTGGCGAgcgcacg > 1:89279/1‑83 (MQ=255) ttttcTGCCTGCAGCCGTCGGCCCTGGTCAACGTGCGCACTGGCGAGCGC‑CGTGCGCTCGACCATTTCCCCGCCGGCCAGCGCCTGCATGCGGTGGCCGGTATCGGCAACCCGCAACGTTTCTTCAACACCCTGCTGGGGCTAAACTGGc < 2:334098/149‑1 (MQ=255) cctgcAGCCGTCGGCCCTGGTCAACGTGCGCACTGGCGAGCGC‑CGTGCGCTCGACCATTTCCCCGCCGGCCAGCGCCTGCATGCGGTGGCCGGTATCGGCAACCCGCAACGTTTCTTCAACACCCTGCTGGGGCTAAACTGGCAGCCGGt > 2:108851/1‑150 (MQ=255) gTCGGCCCTGGTCAACGTGCGCACTGGCGAGCGC‑CGTGCGCTCGACCATTTCCCCGCCGGCCAGCGCCTGCATGCGGTGGCCGGTATCGGCAACCCGCAACGTTTCTTCAACACCCTGCTGGGGCTAAACTGGCAGCCGGTGCCGCATcc < 1:397789/150‑1 (MQ=255) cccTGGTCAACGTGCGCACTGGCGAGCGC‑CGTGCGCTCGACCATTTCCCCGCCGGCCAGCGCCTGCATGCGGTGGCCGGTATCGGCAACCCGCAACGTTTCTTCAACACCCTGCTGGGGCTAAACTGGCAGCCGGTGCCGCATCCCTTCg > 1:94472/1‑150 (MQ=255) ccTGGTCAACGTGCGCACTGGCGAGCGC‑CGTGCGCTCGACCATTTCCCCGCCGGCCAGCGCCTGCATGCGGTGGCCGGTATCGGCAACCCGCAACGTTTCTTCAACACCCTGCTGGGGCTAAACTGGCAGCCGGAGCCGCATCCCTTCGc > 2:117512/1‑150 (MQ=255) tggccggcggggaaatgGTCGAGCGC‑ACGGCGCTCGCCCATTTCCCCGCCGGCCAGCGCCTGCATGCGGTGGCCGGTATCGGCAACCCGCAACGTTTCTTCAACACCCTGCTgggg > 2:265089‑M1/18‑116 (MQ=255) tggccggcggggaaatgGTCGAGCGC‑ACGGCGCTCGCCCATTTCCCCGCCGGCCAGCGCCTGCATGCGGTGGCCGGTATCGGCAACCCGCAACGTTTCTTCAACACCCTGCTgggg < 1:265089‑M1/99‑1 (MQ=255) ggcggggaaatgGTCGAGCGC‑ACGGCGCTCGCCCATTTCCCCGCCGGCCAGCGCCTGCATGCGGTGGCCGGTATCGGCAACCCGCAACGTTTCTTCAACACCCTGCTgggg > 2:360595‑M1/13‑111 (MQ=255) ggcggggaaatgGTCGAGCGC‑ACGGCGCTCGCCCATTTCCCCGCCGGCCAGCGCCTGCATGCGGTGGCCGGTATCGGCAACCCGCAACGTTTCTTCAACACCCTGCTgggg < 1:360595‑M1/99‑1 (MQ=255) cGTGCGCACTGGCGAGCGC‑CGTGCGCTCGACCATTTCCCCGCCGGCCAGCGCCTGCATGCGGTGGCCGGTATCGGCAACCCGCAACGTTTCTTCAACACCCTGCTGGGGCTAAACTGGCAGCCGGTGCCGCATCCCTTCGCCGACCATgc < 1:117512/150‑1 (MQ=255) cGTGCGCACTGGCGAGCGC‑CGTGCGCTCGACCATTTCCCCGCCGGCCAGCGCCTGCATGCGGTGGCCGGTATCGGCAACCCGCAACGTTTCTTCAACACCCTGCTGGGGCTAAACTGGCAGCAGGTGCCGCATCCCTTCGCCGACCATgc < 1:68577/150‑1 (MQ=255) gcgcACTGGCGAGCGC‑CGTGCGCTCGACCATTTCCCCGCCGGCCAGCGCCTGCATGCGGTGGCCGGTATCGGCAACCCGCAACGTTTCTTCAACACCCTGCTGGGGCTAAACTGGCAGCCGGTGCCGCATCCCTTCGCCGACCATGCGCa < 1:398033/150‑1 (MQ=255) atgGTCGAGCGCACG‑GCGCTCGCCCATTTCCCCGCCGGCCAGCGCCTGCATGCGGTGGCCGGTATCGGCAACCCGCAACGTTTCTTCAACACCCTGCTgg > 1:479897‑M1/4‑100 (MQ=255) atgGTCGAGCGCACG‑GCGCTCGCCCATTTCCCCGCCGGCCAGCGCCTGCATGCGGTGGCCGGTATCGGCAACCCGCAACGTTTCTTCAACACCCTGCTgg < 2:479897‑M1/97‑1 (MQ=255) cTGGCGAGCGC‑CGTGCGCTCGACCATTTCCCCGCCGGCCAGCGCCTGCATGCGGTGGCCGGTATCGGCAACCCGCAACGTTTCTTCAACACCCTGCTGGGGCTAAACTGGCAGCCGGTGCCGCATCCCTTCGCCGACCATGCGCAGTTCa < 2:73725/150‑1 (MQ=255) | GGGCCGTTGCGTGAGCCGGCCGACCGCTTGCGTGAGGCTGACGCGGTGCTGTTCAACGGCGCCAGCGAAGACCGCACCGATGGTTTCGGTTTCTGCCTGCAGCCGTCGGCCCTGGTCAACGTGCGCACTGGCGAGCGC‑CGTGCGCTCGACCATTTCCCCGCCGGCCAGCGCCTGCATGCGGTGGCCGGTATCGGCAACCCGCAACGTTTCTTCAACACCCTGCTGGGGCTAAACTGGCAGCCGGTGCCGCATCCCTTCGCCGACCATGCGCAGTTCA > NC_002947/2144244‑2144520 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |