Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,508,883 A→G 12.9% V92A (GTG→GCG)  PP_2203 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,508,8830AG12.9% 74.0 / 3.8 31V92A (GTG→GCG) PP_2203hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (15/12);  new base G (2/2);  total (17/14)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.42e-01

CACGGTGTCCTGGCCATCCTGGGTCACCCCGATGACCTGGTAGGCATCCTTGTGGTCACCCATCTCCATGCCGGGCGAGCCCATGGGCATGCCCGGCACCGCCAGGCCCTTGAGGTCGTTGCGTTTGGCCAGCAGGCGCACCTGCTCGGCCGGCACGTGGCCTTCGACGAACTTGCCGCCGATCACCCCGGTGTGGCACGACGCAAGGCGTGGCGCCACGCCGAGGCGTTGCTTGACGGCGCTCATGTTTGGCTCGACGTGGTCGTTGACGG  >  NC_002947/2508744‑2509015
                                                                                                                                           |                                                                                                                                    
cACGGTGTCCTGGCCATCCTGGGTCACCCCGATGACCTGGTAGGCATCCTTGTGGTCACCCATCTCCATGCCGGGCGAGCCCATGGGCATGCCCGGCACCGCCAGGCCCTTGAGGTCGTTGCGTTTGGCCAGCAGGCGCACCTGCTcggc                                                                                                                            >  2:341834/1‑150 (MQ=255)
         cTGGCCATCCTGGGTCACCCCGATGACCTGGTAGGCATCCTTGTGGTCACCCATCTCCATGCCGGGCGAGCCCATGGGCATGCCCTGCACCGCCAGGCCCTTGAGGTCGTTGCGTTTGGCCAGCAGGCGCACCTGCTCGGCCGGCACGTg                                                                                                                   >  2:150882/1‑150 (MQ=255)
         cTGGCCATCCTGGGTCACCCCGATGACATGGTAGGCATCATTGTGGTAGCTCATCTACGTCGCTAGCTAGCCCATGGGCATGCCCGGCACCGCCAGGCCCTTGAGGTCGTTGCGTTTGGCCAGCAGGCGCACCTGCTCGGCCGGCACGTg                                                                                                                   >  2:439842/1‑150 (MQ=255)
          tGGCCATCCTGGGTCACCCCGATGACCTGGTAGGCATCCTTGTGGTCACCCATCTCCATGCCGGGCGAGCCCATGGGCATGCCCGGCACCGCCAGGCCCTTGAGGTCGTTGCGTTTGGCCAGCAGGCGCACCTGCTCGGCCGGCACGTgg                                                                                                                  <  2:460653/150‑1 (MQ=255)
             ccATCCTGGGTCACCCCGATGACCTGGTAGGCATCCTTGTGGTCACCCATCTCCATGCCGGGCGAGCCCATGGGCATGCCCGGCACCGCCAGGCCCTTGAGGTCGTTGCGTTTGGCCAGCAGGCGCACCTGCTCGGCCGGCACGTGGCCt                                                                                                               >  2:195885/1‑150 (MQ=255)
                             cGATGACCTGGTAGGCATCCTTGTGGTCACCCATCTCCATGCCGGGCGAGCCCATGGGCATGCCCGGCACCGCCAGGCCCTTGAGGTCGTTGCGTTTGGCCAGCAGGCGCACCTGCTCGGCCGGCACGTGGCCTTCGACGAACTTgccgc                                                                                               >  1:307056/1‑150 (MQ=255)
                                      ggTAGGCATCCTTGTGGTCACCCATCTCCATGCCGGGCGAGCCCATGGGCATGCCCTGCACCGCCAGGCCCTTGAGGTCGTTGCGTTTGGCCAGCAGGCGCACCTGCTCGGCCGGCACGTGGCCTTCGACGAACTTGCCGCCGATCAccc                                                                                      <  1:150882/150‑1 (MQ=255)
                                                 ttGTGGTCACCCATCTCCATGCCGGGCGAGCCCATGGGCATGCCCGGCACCGCCAGGCCCTTGAGGTCGTTGCGTTTGGCCAGCAGGCGCACCTGCTCGGCCGGCACGTGGCCTTCGACGAACTTGCCGCCGATCACCCCGGTGTGGCac                                                                           >  1:221419/1‑150 (MQ=255)
                                                       tCACCCATCTCCATGCCGGGCGAGCCCATGGGCATGCCCGGCACCGCCAGGCCCTTGAGGTCGTTGCGTTTGGCCAGCAGGCGCACCTGCTCGGCCGGCACGTGGCCTTCGACGAACTTGCCGCCGATCACCCCGGTGTGGCACGACGCa                                                                     >  2:135664/1‑150 (MQ=255)
                                                             aTCTCCATGCCGGGCGAGCCCATGGGCATGCCCGGCACCGCCAGGCCCTTGAGGTCGTTGCGTTTGGCCAGCAGGCGCACCTGCTCGGCCGGCACGTGGCCTTCGACGAACTTGCCGCCGATCACCCCGGTGTGGCac                                                                           <  1:122511/138‑1 (MQ=255)
                                                             aTCTCCATGCCGGGCGAGCCCATGGGCATGCCCGGCACCGCCAGGCCCTTGAGGTCGTTGCGTTTGGCCAGCAGGCGCACCTGCTCGGCCGGCACGTGGCCTTCGACGAACTTGCCGCCGATCACCCCGGTGTGGCac                                                                           >  2:122511/1‑138 (MQ=255)
                                                                  cATGCCGGGCGAGCCCATGGGCATGCCCGGCACCGCCAGGCCCTTGAGGTCGTTGCGTTTGGCCAGCAGGCGCACCTGCTCGGCCGGCACGTGGCCTTCGACGAACTTGCCGCCGATCACCCCGGTGTGGCACGACGCAAGGCGTGgcgc                                                          >  2:278037/1‑150 (MQ=255)
                                                                  cATGCCGGGCGAGCCCATGGGCATGCCCGGCACCGCCAGGCCCTTGAGGTCGTTGCGTTTGGCCAGCAGGCGCACCTGCTCGGCCGGCACGTGGCCTTCGACGAACTTGCCGCCGATCACCCCGGTGTGGCACGACGCAAAGCGttctgc                                                          <  2:307056/150‑6 (MQ=255)
                                                                        gggCGAGCCCATGGGCATGCCCGGCACCGCCAGGCCCTTGAGGTCGTTGCGTTTGGCCAGCAGGCGCACCTGCTCGGCCGGCACGTGGCCTTCGACGAACTTGCCGCCGATCACCCCGGTGTGGCACGACGCAAGGCGTGGCGCCACGcc                                                    <  1:135664/150‑1 (MQ=255)
                                                                               cccATGGGCATGCCCGGCACCGCCAGGCCCTTGAGGTCGTTGCGTTTGGCCAGCAGGCGCACCTGCTCGGCCGGCACGTGGCCTTCGACGAACTTGCCGCCGATCACCCCGGTGTGGCACGACGCAAGGCGTGGCGCCACGCCGAGGCGt                                             <  1:439842/150‑1 (MQ=255)
                                                                                ccATGGGCATGCCCGGCACCGCCAGGCCCTTGAGGTCGTTGCGTTTGGCCAGCAGGCGCACCTGc                                                                                                                                 >  2:9301/1‑65 (MQ=255)
                                                                                ccATGGGCATGCCCGGCACCGCCAGGCCCTTGAGGTCGTTGCGTTTGGCCAGCAGGCGCACCTGc                                                                                                                                 <  1:9301/65‑1 (MQ=255)
                                                                                      gCATGCCCGGCACCGCCAGGCCCTTGAGGTCGTTGCGTTTGGCCAGCAGGCGCACCTGCTCGGCCGGCACGTGGCCTTCGACGAACTTGCCGCCGATCACCCCGGTGTGGCACGACGCAAGGCGTGGCGCCACGCCGAGGCGTTGCTTGa                                      <  2:221419/150‑1 (MQ=255)
                                                                                        aTGCCCGGCACCGCCAGGCCCTTGAGGTCGTTGCGTTTGGCCAGCAGGCGCACCTGCTCGGCCGGCACGTGACCTTCGACGAACTTGCCGCCGATCACCCCGAGGTGGCACGACGCAAGGCGTGGCGACACGCCGAGGCGTTGCTTGACg                                    >  2:222727/1‑150 (MQ=255)
                                                                                            ccGGCACCGCCAGGCCCTTGAGGTCGTTGCGTTTGGCCAGCAGGCGCACCTGCTCGGCCGGCACGTGGCCTTCGACGAACTTGCCGCCGATCAccc                                                                                      <  2:245114/96‑1 (MQ=255)
                                                                                            ccGGCACCGCCAGGCCCTTGAGGTCGTTGCGTTTGGCCAGCAGGCGCACCTGCTCGGCCGGCACGTGGCCTTCGACGAACTTGCCGCCGATCAccc                                                                                      >  1:245114/1‑96 (MQ=255)
                                                                                               gCACCGCCAGGCCCTTGAGGTCGTTGCGTTTGGCCAGCAGGCGCACCTGCTCGGCCGGCACGTGGCCTTCGACGAACTTGCCGCCGATCACCCCGGTGTGGCACGACGCAAGGCGTGgc                                                            <  1:440714/119‑1 (MQ=255)
                                                                                               gCACCGCCAGGCCCTTGAGGTCGTTGCGTTTGGCCAGCAGGCGCACCTGCTCGGCCGGCACGTGGCCTTCGACGAACTTGCCGCCGATCACCCCGGTGTGGCACGACGCAAGGCGTGgc                                                            >  2:440714/1‑119 (MQ=255)
                                                                                                            cTTGAGGTCGTTGCGTTTGGCCAGCAGGCGCACCTGCTCGGCCGGCACGTGGCCTTCGACGAACTTGCCGCCGATCACCCCGGTGTGGCACGACGCAAGGCGTGGCGCCACGCCGAGGCGTTGCTTGACGGCGCTCATGTTTGGCTCGAc                >  1:462327/1‑150 (MQ=255)
                                                                                                               gAGGTCGTTGCGTTTGGCCAGCAGGCGCACCTGCTCGGCCGGCACGTGGCCTTCGACGAACTTGCCGCCGATCACCCCGGTGTGGCACGACGCAAGGCGTGGCGCCACGCCGAGGCGTTGCTTGACGGCGCTCATGTTTGGCTCGACGTg             >  2:233854/1‑150 (MQ=255)
                                                                                                                  gTCGTTGCGTTTGGCCAGCAGGCGCACCTGCTCGGCCGGCACGTGGCCTTCGACGAACTTGCCGCCGATCACCCCGGTGTGGCACGACGCAAGGCGTGGCGCCACGCCGAGGCGTTGCTTGACGGCGCTCATGTTTGGCTCGACGTGGTc          <  1:233854/150‑1 (MQ=255)
                                                                                                                          ggttGGCCAGCAGGCCCACCTGCTCGGCCGGCACGTGGCCTTCGACGAACTTGCCGCCGATCCCCCCGGTGTGGCACGACGCAAGGCGTGGCGCCACGCCGAGGCGTTGCTTGACGGCGCTCATGTTTGGCTCGACGTGGTCGTTGACgg  <  1:341834/148‑1 (MQ=255)
                                                                                                                             ggccgagcagGTGCGCCTGCT‑GGCCGGCACGTGGCCTTCGACGAACTTGCCGCCGATCACCCCGGTGTGGCACGACGCAAGGCGTGGCGCCACGCCGAGGCGTTGCt                                         >  1:356449/6‑107 (MQ=255)
                                                                                                                             ggccgagcagGTGCGCCTGCT‑GGCCGGCACGTGGCCTTCGACGAACTTGCCGCCGATCACCCCGGTGTGGCACGACGCAAGGCGTGGCGCCACGCCGAGGCGTTGCt                                         <  2:356449/102‑1 (MQ=255)
                                                                                                                              gccgagcagGTGCGCCTGCTCGGCCGGCACGTGGCCTTCGACGAACTTGCCGCCGATCACCCCGGTGTGGCACGACGCAAGGCGTGGCGCCACGCCGAg                                                 >  2:466415/5‑99 (MQ=255)
                                                                                                                              gccgagcagGTGCGCCTGCTCGGCCGGCACGTGGCCTTCGACGAACTTGCCGCCGATCACCCCGGTGTGGCACGACGCAAGGCGTGGCGCCACGCCGAg                                                 <  1:466415/95‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                    
CACGGTGTCCTGGCCATCCTGGGTCACCCCGATGACCTGGTAGGCATCCTTGTGGTCACCCATCTCCATGCCGGGCGAGCCCATGGGCATGCCCGGCACCGCCAGGCCCTTGAGGTCGTTGCGTTTGGCCAGCAGGCGCACCTGCTCGGCCGGCACGTGGCCTTCGACGAACTTGCCGCCGATCACCCCGGTGTGGCACGACGCAAGGCGTGGCGCCACGCCGAGGCGTTGCTTGACGGCGCTCATGTTTGGCTCGACGTGGTCGTTGACGG  >  NC_002947/2508744‑2509015

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑